Incidental Mutation 'R5637:Gpr158'
| ID |
440406 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Gpr158
|
| Ensembl Gene |
ENSMUSG00000045967 |
| Gene Name |
G protein-coupled receptor 158 |
| Synonyms |
5330427M13Rik |
| Accession Numbers |
|
| Essential gene? |
Non essential
(E-score: 0.000)
|
| Stock # |
R5637 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
2 |
| Chromosomal Location |
21372378-21835355 bp(+) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to G
at 21788083 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Isoleucine to Valine
at position 575
(I575V)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000049708
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000055946]
|
| AlphaFold |
Q8C419 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000055946
AA Change: I575V
PolyPhen 2
Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)
|
| SMART Domains |
Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: I575V
| Domain | Start | End | E-Value | Type |
|---|
|
signal peptide
|
1 |
20 |
N/A |
INTRINSIC |
|
low complexity region
|
110 |
125 |
N/A |
INTRINSIC |
|
SCOP:d1edmb_
|
313 |
359 |
5e-4 |
SMART |
|
Blast:EGF
|
318 |
365 |
2e-27 |
BLAST |
|
Pfam:7tm_3
|
426 |
669 |
1.2e-35 |
PFAM |
|
low complexity region
|
840 |
863 |
N/A |
INTRINSIC |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000155760
|
| Coding Region Coverage |
- 1x: 99.2%
- 3x: 98.5%
- 10x: 96.9%
- 20x: 94.2%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 63 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acmsd |
T |
C |
1: 127,694,050 (GRCm39) |
F327L |
probably damaging |
Het |
| Adgrl3 |
C |
A |
5: 81,841,391 (GRCm39) |
S824Y |
probably damaging |
Het |
| Arfip2 |
A |
G |
7: 105,286,370 (GRCm39) |
M144T |
probably damaging |
Het |
| Arhgap32 |
A |
G |
9: 32,158,502 (GRCm39) |
N179S |
probably damaging |
Het |
| Ash2l |
T |
C |
8: 26,317,339 (GRCm39) |
Y249C |
probably damaging |
Het |
| Ccndbp1 |
A |
G |
2: 120,842,165 (GRCm39) |
T141A |
probably benign |
Het |
| Cd177 |
T |
A |
7: 24,455,748 (GRCm39) |
H258L |
probably benign |
Het |
| Celsr3 |
T |
C |
9: 108,714,332 (GRCm39) |
W1956R |
probably damaging |
Het |
| Cep295 |
T |
C |
9: 15,245,108 (GRCm39) |
|
probably null |
Het |
| Cngb1 |
T |
C |
8: 95,984,549 (GRCm39) |
H420R |
probably damaging |
Het |
| Cobl |
C |
T |
11: 12,246,531 (GRCm39) |
|
probably benign |
Het |
| Cobll1 |
T |
C |
2: 64,956,247 (GRCm39) |
D337G |
possibly damaging |
Het |
| Dmrta1 |
A |
G |
4: 89,577,068 (GRCm39) |
N175D |
probably benign |
Het |
| Dnah7b |
G |
T |
1: 46,395,674 (GRCm39) |
V3859L |
possibly damaging |
Het |
| Dnah7c |
A |
G |
1: 46,799,521 (GRCm39) |
|
probably null |
Het |
| Dusp4 |
C |
A |
8: 35,284,451 (GRCm39) |
H255Q |
probably damaging |
Het |
| Evx1 |
T |
C |
6: 52,292,751 (GRCm39) |
V134A |
possibly damaging |
Het |
| F12 |
T |
C |
13: 55,570,228 (GRCm39) |
K93E |
possibly damaging |
Het |
| Fam186a |
A |
C |
15: 99,839,628 (GRCm39) |
H2205Q |
possibly damaging |
Het |
| Fcgbpl1 |
A |
G |
7: 27,852,277 (GRCm39) |
N1267D |
probably benign |
Het |
| Frem2 |
A |
G |
3: 53,560,358 (GRCm39) |
I1383T |
probably damaging |
Het |
| Gm11595 |
G |
A |
11: 99,663,381 (GRCm39) |
R100C |
unknown |
Het |
| Hdc |
A |
G |
2: 126,458,109 (GRCm39) |
V71A |
probably benign |
Het |
| Helb |
G |
A |
10: 119,941,353 (GRCm39) |
T445M |
probably benign |
Het |
| Inppl1 |
A |
T |
7: 101,478,055 (GRCm39) |
S652R |
probably benign |
Het |
| Itpripl1 |
T |
C |
2: 126,984,044 (GRCm39) |
D26G |
probably damaging |
Het |
| Klc1 |
T |
A |
12: 111,740,842 (GRCm39) |
L106H |
probably damaging |
Het |
| Klhl25 |
T |
A |
7: 75,515,540 (GRCm39) |
|
probably null |
Het |
| Krt6a |
T |
C |
15: 101,600,714 (GRCm39) |
D318G |
probably benign |
Het |
| Lrp2 |
T |
C |
2: 69,302,762 (GRCm39) |
N2989S |
probably damaging |
Het |
| Lta4h |
A |
G |
10: 93,304,731 (GRCm39) |
|
probably null |
Het |
| Man1c1 |
A |
G |
4: 134,318,735 (GRCm39) |
S251P |
probably damaging |
Het |
| Mapre2 |
T |
C |
18: 23,886,919 (GRCm39) |
|
probably benign |
Het |
| Mfap3l |
A |
T |
8: 61,109,821 (GRCm39) |
I66F |
probably damaging |
Het |
| Mvk |
A |
G |
5: 114,594,003 (GRCm39) |
E286G |
possibly damaging |
Het |
| Nos1ap |
T |
A |
1: 170,176,968 (GRCm39) |
K145M |
probably damaging |
Het |
| Or14a260 |
G |
A |
7: 85,984,812 (GRCm39) |
T264I |
probably benign |
Het |
| Or4f52 |
T |
A |
2: 111,061,456 (GRCm39) |
K227N |
probably benign |
Het |
| Or7d11 |
A |
T |
9: 19,966,279 (GRCm39) |
V160D |
possibly damaging |
Het |
| Pcdh9 |
A |
G |
14: 94,123,198 (GRCm39) |
F991L |
possibly damaging |
Het |
| Pcdha9 |
T |
C |
18: 37,131,426 (GRCm39) |
V165A |
probably benign |
Het |
| Pcsk6 |
A |
G |
7: 65,618,745 (GRCm39) |
H437R |
probably damaging |
Het |
| Pfas |
C |
T |
11: 68,884,149 (GRCm39) |
V589M |
probably damaging |
Het |
| Prpf40a |
A |
G |
2: 53,046,746 (GRCm39) |
V288A |
possibly damaging |
Het |
| Rnf183 |
T |
C |
4: 62,346,387 (GRCm39) |
D137G |
probably benign |
Het |
| Rsph6a |
A |
G |
7: 18,788,820 (GRCm39) |
S51G |
probably benign |
Het |
| Scap |
G |
A |
9: 110,210,640 (GRCm39) |
A991T |
possibly damaging |
Het |
| Sdk2 |
C |
A |
11: 113,724,005 (GRCm39) |
V1222F |
probably damaging |
Het |
| Sdr16c6 |
T |
A |
4: 4,063,232 (GRCm39) |
N181I |
possibly damaging |
Het |
| Semp2l2a |
A |
T |
8: 13,887,713 (GRCm39) |
M126K |
possibly damaging |
Het |
| Serpinb7 |
A |
T |
1: 107,356,037 (GRCm39) |
D20V |
probably damaging |
Het |
| Sh3bp2 |
C |
A |
5: 34,718,392 (GRCm39) |
R531S |
possibly damaging |
Het |
| Skint8 |
C |
A |
4: 111,807,390 (GRCm39) |
L359M |
probably damaging |
Het |
| Sox2 |
A |
G |
3: 34,704,677 (GRCm39) |
N38S |
probably benign |
Het |
| Spg7 |
C |
A |
8: 123,821,314 (GRCm39) |
Q680K |
possibly damaging |
Het |
| Styk1 |
T |
C |
6: 131,277,381 (GRCm39) |
E331G |
possibly damaging |
Het |
| Tkfc |
G |
A |
19: 10,571,897 (GRCm39) |
R380W |
probably benign |
Het |
| Trib3 |
A |
G |
2: 152,180,410 (GRCm39) |
F261S |
probably damaging |
Het |
| Ubr1 |
T |
A |
2: 120,793,998 (GRCm39) |
Q62L |
possibly damaging |
Het |
| Vmn2r54 |
T |
C |
7: 12,349,296 (GRCm39) |
Y762C |
probably benign |
Het |
| Vmn2r97 |
T |
A |
17: 19,167,628 (GRCm39) |
Y627* |
probably null |
Het |
| Zfp12 |
A |
G |
5: 143,231,451 (GRCm39) |
K625E |
probably damaging |
Het |
| Zw10 |
T |
C |
9: 48,968,950 (GRCm39) |
V38A |
probably damaging |
Het |
|
| Other mutations in Gpr158 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00340:Gpr158
|
APN |
2 |
21,373,494 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00469:Gpr158
|
APN |
2 |
21,751,606 (GRCm39) |
splice site |
probably benign |
|
|
IGL00706:Gpr158
|
APN |
2 |
21,751,584 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL00780:Gpr158
|
APN |
2 |
21,831,629 (GRCm39) |
nonsense |
probably null |
|
|
IGL00885:Gpr158
|
APN |
2 |
21,653,832 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL01339:Gpr158
|
APN |
2 |
21,373,842 (GRCm39) |
missense |
possibly damaging |
0.73 |
|
IGL01368:Gpr158
|
APN |
2 |
21,831,909 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02141:Gpr158
|
APN |
2 |
21,788,101 (GRCm39) |
missense |
probably damaging |
0.99 |
|
IGL02455:Gpr158
|
APN |
2 |
21,373,511 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL02554:Gpr158
|
APN |
2 |
21,831,407 (GRCm39) |
missense |
probably benign |
|
|
IGL02681:Gpr158
|
APN |
2 |
21,820,441 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL02752:Gpr158
|
APN |
2 |
21,831,638 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
IGL02756:Gpr158
|
APN |
2 |
21,831,890 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
IGL03181:Gpr158
|
APN |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
0.02 |
|
IGL03258:Gpr158
|
APN |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
IGL03386:Gpr158
|
APN |
2 |
21,831,057 (GRCm39) |
missense |
probably damaging |
1.00 |
|
PIT4810001:Gpr158
|
UTSW |
2 |
21,831,682 (GRCm39) |
missense |
probably benign |
0.01 |
|
R0071:Gpr158
|
UTSW |
2 |
21,815,479 (GRCm39) |
missense |
probably benign |
0.08 |
|
R0081:Gpr158
|
UTSW |
2 |
21,831,528 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0528:Gpr158
|
UTSW |
2 |
21,830,019 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0560:Gpr158
|
UTSW |
2 |
21,830,085 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0603:Gpr158
|
UTSW |
2 |
21,820,480 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R1560:Gpr158
|
UTSW |
2 |
21,831,125 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1561:Gpr158
|
UTSW |
2 |
21,820,505 (GRCm39) |
splice site |
probably null |
|
|
R1609:Gpr158
|
UTSW |
2 |
21,788,104 (GRCm39) |
missense |
possibly damaging |
0.61 |
|
R1741:Gpr158
|
UTSW |
2 |
21,832,359 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1827:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R1854:Gpr158
|
UTSW |
2 |
21,373,935 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1871:Gpr158
|
UTSW |
2 |
21,820,426 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2151:Gpr158
|
UTSW |
2 |
21,832,325 (GRCm39) |
missense |
possibly damaging |
0.82 |
|
R2273:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R2275:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R3004:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R3151:Gpr158
|
UTSW |
2 |
21,581,771 (GRCm39) |
missense |
possibly damaging |
0.68 |
|
R3943:Gpr158
|
UTSW |
2 |
21,373,370 (GRCm39) |
missense |
possibly damaging |
0.65 |
|
R4238:Gpr158
|
UTSW |
2 |
21,373,362 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4379:Gpr158
|
UTSW |
2 |
21,830,025 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4381:Gpr158
|
UTSW |
2 |
21,832,403 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4464:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4467:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4496:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4506:Gpr158
|
UTSW |
2 |
21,831,810 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R4530:Gpr158
|
UTSW |
2 |
21,373,811 (GRCm39) |
missense |
probably benign |
0.03 |
|
R4646:Gpr158
|
UTSW |
2 |
21,831,864 (GRCm39) |
missense |
probably benign |
|
|
R4798:Gpr158
|
UTSW |
2 |
21,787,993 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R4882:Gpr158
|
UTSW |
2 |
21,830,059 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R4943:Gpr158
|
UTSW |
2 |
21,831,968 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5334:Gpr158
|
UTSW |
2 |
21,832,316 (GRCm39) |
missense |
probably benign |
0.01 |
|
R5560:Gpr158
|
UTSW |
2 |
21,831,101 (GRCm39) |
missense |
possibly damaging |
0.67 |
|
R5600:Gpr158
|
UTSW |
2 |
21,832,046 (GRCm39) |
missense |
probably benign |
|
|
R5701:Gpr158
|
UTSW |
2 |
21,751,520 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5744:Gpr158
|
UTSW |
2 |
21,373,331 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5911:Gpr158
|
UTSW |
2 |
21,373,932 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R5991:Gpr158
|
UTSW |
2 |
21,373,319 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R6200:Gpr158
|
UTSW |
2 |
21,404,227 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R6306:Gpr158
|
UTSW |
2 |
21,820,422 (GRCm39) |
missense |
possibly damaging |
0.84 |
|
R6324:Gpr158
|
UTSW |
2 |
21,815,365 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6384:Gpr158
|
UTSW |
2 |
21,831,099 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6698:Gpr158
|
UTSW |
2 |
21,831,921 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R6997:Gpr158
|
UTSW |
2 |
21,653,802 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R7086:Gpr158
|
UTSW |
2 |
21,831,386 (GRCm39) |
missense |
probably benign |
0.01 |
|
R7175:Gpr158
|
UTSW |
2 |
21,373,113 (GRCm39) |
missense |
probably benign |
0.13 |
|
R7197:Gpr158
|
UTSW |
2 |
21,815,412 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7293:Gpr158
|
UTSW |
2 |
21,581,750 (GRCm39) |
missense |
possibly damaging |
0.47 |
|
R7427:Gpr158
|
UTSW |
2 |
21,832,129 (GRCm39) |
missense |
probably benign |
|
|
R7515:Gpr158
|
UTSW |
2 |
21,373,092 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7730:Gpr158
|
UTSW |
2 |
21,831,158 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8122:Gpr158
|
UTSW |
2 |
21,831,674 (GRCm39) |
missense |
probably benign |
|
|
R8311:Gpr158
|
UTSW |
2 |
21,373,701 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8754:Gpr158
|
UTSW |
2 |
21,581,693 (GRCm39) |
missense |
probably benign |
0.00 |
|
R8782:Gpr158
|
UTSW |
2 |
21,404,149 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8792:Gpr158
|
UTSW |
2 |
21,558,137 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8842:Gpr158
|
UTSW |
2 |
21,581,751 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R9009:Gpr158
|
UTSW |
2 |
21,581,760 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9102:Gpr158
|
UTSW |
2 |
21,830,078 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9150:Gpr158
|
UTSW |
2 |
21,831,251 (GRCm39) |
missense |
probably benign |
0.17 |
|
R9254:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9317:Gpr158
|
UTSW |
2 |
21,832,037 (GRCm39) |
missense |
probably benign |
|
|
R9379:Gpr158
|
UTSW |
2 |
21,373,042 (GRCm39) |
start gained |
probably benign |
|
|
R9428:Gpr158
|
UTSW |
2 |
21,787,972 (GRCm39) |
missense |
probably benign |
|
|
R9497:Gpr158
|
UTSW |
2 |
21,831,825 (GRCm39) |
missense |
probably benign |
0.00 |
|
R9667:Gpr158
|
UTSW |
2 |
21,830,054 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9681:Gpr158
|
UTSW |
2 |
21,831,315 (GRCm39) |
missense |
probably damaging |
0.99 |
|
X0062:Gpr158
|
UTSW |
2 |
21,831,180 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1176:Gpr158
|
UTSW |
2 |
21,815,501 (GRCm39) |
critical splice donor site |
probably null |
|
|
Z1177:Gpr158
|
UTSW |
2 |
21,832,083 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
| Predicted Primers |
PCR Primer
(F):5'- AATCCCATACATGACTGGTGG -3'
(R):5'- CTGCACATAGATGTTGCCTTTC -3'
Sequencing Primer
(F):5'- GTCGGGTCATGAGGATGC -3'
(R):5'- ATGAGTTTTTGTTTGCATTCCAC -3'
|
| Posted On |
2016-11-08 |
Incidental Mutation