Mutagenetix > Incidental Mutation

Incidental Mutation 'R5640:Vmn2r107'

440637

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r107

Ensembl Gene

ENSMUSG00000056910

Gene Name

vomeronasal 2, receptor 107

Synonyms

V2r6

MMRRC Submission

043289-MU

Accession Numbers

Genbank: NM_001104569; MGI: 1316664

Essential gene?

Probably non essential (E-score: 0.085) question?

Stock #

R5640 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20345425-20375772 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20375164 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 660 (T660A)

Ref Sequence

ENSEMBL: ENSMUSP00000048706 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000042090]

AlphaFold

E9PZJ7

Predicted Effect

probably damaging
Transcript: ENSMUST00000042090
AA Change: T660A

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000048706
Gene: ENSMUSG00000056910
AA Change: T660A

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	466	3.6e-40	PFAM
Pfam:NCD3G	509	562	5.1e-21	PFAM
Pfam:7tm_3	593	830	8e-51	PFAM

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.5%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 62 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ace	A	G	11: 105,970,685	Y220C	probably damaging	Het
Actl6a	A	G	3: 32,718,050	T170A	probably damaging	Het
Adamts8	T	C	9: 30,956,500	V540A	probably benign	Het
Aga	T	C	8: 53,511,884	L27P	probably damaging	Het
Agmat	T	A	4: 141,755,823	H189Q	probably damaging	Het
Alx3	C	A	3: 107,600,661	T162K	probably damaging	Het
Armc2	T	A	10: 42,011,898	I30L	possibly damaging	Het
Arntl	C	T	7: 113,308,681	P530L	probably damaging	Het
Atp10d	T	G	5: 72,247,209	Y487D	probably damaging	Het
Atp13a4	C	T	16: 29,415,831	M908I	probably damaging	Het
B4galt2	T	G	4: 117,873,998	N322T	probably benign	Het
Brdt	A	T	5: 107,359,308	K525*	probably null	Het
Ces3a	T	A	8: 105,051,745	M246K	probably benign	Het
Chd9	T	A	8: 91,036,562	H2338Q	probably damaging	Het
Cyp3a16	T	A	5: 145,452,823	D244V	possibly damaging	Het
Dhrs9	C	A	2: 69,394,478	A170E	probably damaging	Het
Dlg5	T	C	14: 24,170,461	N550D	probably damaging	Het
Dnah8	C	T	17: 30,803,108	T3894I	probably damaging	Het
Dpys	T	C	15: 39,842,066	H217R	probably damaging	Het
Dpysl2	C	T	14: 66,834,368	V108I	probably benign	Het
Eprs	A	G	1: 185,374,184	T199A	probably benign	Het
Fads1	A	G	19: 10,186,403	D183G	probably damaging	Het
Fam110b	T	G	4: 5,798,689	Y36D	probably damaging	Het
Fbxl21	G	A	13: 56,537,381	D407N	probably benign	Het
Fuca2	G	A	10: 13,507,430		probably null	Het
Gm8251	T	C	1: 44,061,927	I4V	probably benign	Het
Gnas	C	A	2: 174,284,971	R100S	probably benign	Het
Gse1	A	G	8: 120,562,677	H90R	possibly damaging	Het
Hoxc10	G	T	15: 102,967,267	C137F	probably benign	Het
Ipp	T	G	4: 116,520,689	L252R	possibly damaging	Het
Jmjd1c	T	A	10: 67,226,078	S1403R	probably benign	Het
Kif19a	A	G	11: 114,779,215	M79V	probably benign	Het
Lipo4	T	C	19: 33,501,586	T285A	possibly damaging	Het
Lrrc66	A	T	5: 73,608,634	D355E	probably benign	Het
Lrsam1	T	A	2: 32,945,852	Q301L	probably benign	Het
Med6	C	T	12: 81,581,854	R138Q	probably damaging	Het
Nlrc5	A	G	8: 94,475,793	T174A	probably benign	Het
Nrbp1	C	T	5: 31,249,585	R322W	possibly damaging	Het
Olfr1256	T	A	2: 89,835,938	E2D	probably benign	Het
Olfr1338	T	A	4: 118,753,789	T250S	probably benign	Het
Olfr739	C	A	14: 50,424,654	A45D	probably benign	Het
Pde3a	A	G	6: 141,483,915	E734G	probably damaging	Het
Pnpla7	C	T	2: 25,003,001	T167I	possibly damaging	Het
Pop7	T	C	5: 137,502,059	N4S	possibly damaging	Het
Ppm1h	C	A	10: 122,782,278	P114Q	probably benign	Het
Prdm16	T	C	4: 154,341,910	T473A	probably benign	Het
Prdm6	T	C	18: 53,536,741		probably null	Het
Prkdc	T	G	16: 15,829,769	W3686G	possibly damaging	Het
Ptchd4	C	A	17: 42,503,135	H642Q	possibly damaging	Het
Rad1	A	G	15: 10,495,923	Y228C	possibly damaging	Het
Rgs22	G	A	15: 36,106,955	T56I	probably benign	Het
Rnf135	C	A	11: 80,193,907	H169N	probably benign	Het
Rnft1	C	T	11: 86,486,493	Q128*	probably null	Het
Sez6	T	C	11: 77,973,759		probably benign	Het
Sh3rf3	A	G	10: 58,813,947	S125G	probably benign	Het
Sik2	T	C	9: 50,915,506	E295G	possibly damaging	Het
Themis	A	G	10: 28,863,376	Q614R	probably damaging	Het
Thsd7b	T	A	1: 130,116,671	C1129*	probably null	Het
Tmem68	A	T	4: 3,569,512	F59L	probably benign	Het
Tmem8	C	T	17: 26,118,872	T410I	possibly damaging	Het
Vwa5b2	G	T	16: 20,597,542	C484F	probably damaging	Het
Zfp418	T	A	7: 7,181,981	C314*	probably null	Het

Other mutations in Vmn2r107

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01402:Vmn2r107	APN	17	20375747	missense	probably damaging	0.98
IGL01768:Vmn2r107	APN	17	20345606	missense	probably benign	0.32
IGL02086:Vmn2r107	APN	17	20357800	missense	probably benign	0.00
IGL02136:Vmn2r107	APN	17	20374906	missense	probably benign	0.02
IGL02266:Vmn2r107	APN	17	20356777	missense	probably damaging	1.00
IGL02285:Vmn2r107	APN	17	20375561	missense	probably damaging	1.00
IGL02724:Vmn2r107	APN	17	20356744	missense	possibly damaging	0.49
IGL02998:Vmn2r107	APN	17	20357755	missense	probably damaging	0.99
IGL03089:Vmn2r107	APN	17	20375712	missense	probably benign	0.05
IGL03284:Vmn2r107	APN	17	20356911	missense	probably benign	0.07
IGL03307:Vmn2r107	APN	17	20356776	missense	probably benign	0.09
IGL03399:Vmn2r107	APN	17	20357958	splice site	probably benign
3-1:Vmn2r107	UTSW	17	20345504	missense	probably benign
BB006:Vmn2r107	UTSW	17	20345444	missense	probably null	0.96
BB016:Vmn2r107	UTSW	17	20345444	missense	probably null	0.96
R0285:Vmn2r107	UTSW	17	20345611	missense	probably benign	0.00
R0455:Vmn2r107	UTSW	17	20374823	splice site	probably benign
R0497:Vmn2r107	UTSW	17	20375132	missense	probably damaging	1.00
R0506:Vmn2r107	UTSW	17	20357759	missense	probably benign
R0621:Vmn2r107	UTSW	17	20374990	missense	probably benign	0.01
R0667:Vmn2r107	UTSW	17	20355654	missense	possibly damaging	0.91
R1118:Vmn2r107	UTSW	17	20356598	missense	probably benign	0.03
R1204:Vmn2r107	UTSW	17	20357769	missense	probably benign
R1237:Vmn2r107	UTSW	17	20356685	nonsense	probably null
R1485:Vmn2r107	UTSW	17	20374847	missense	possibly damaging	0.95
R1783:Vmn2r107	UTSW	17	20356513	missense	possibly damaging	0.51
R1873:Vmn2r107	UTSW	17	20345578	missense	probably benign	0.10
R1974:Vmn2r107	UTSW	17	20355617	splice site	probably null
R2009:Vmn2r107	UTSW	17	20375467	missense	probably benign	0.01
R2029:Vmn2r107	UTSW	17	20375287	missense	probably benign	0.01
R2164:Vmn2r107	UTSW	17	20375642	missense	probably damaging	1.00
R2269:Vmn2r107	UTSW	17	20375555	missense	possibly damaging	0.58
R3087:Vmn2r107	UTSW	17	20360345	missense	probably benign	0.03
R3740:Vmn2r107	UTSW	17	20374889	missense	probably benign	0.00
R3961:Vmn2r107	UTSW	17	20375455	missense	probably damaging	1.00
R4031:Vmn2r107	UTSW	17	20375221	missense	probably benign	0.00
R4270:Vmn2r107	UTSW	17	20355779	missense	probably benign
R4963:Vmn2r107	UTSW	17	20375141	missense	probably damaging	1.00
R5121:Vmn2r107	UTSW	17	20355753	missense	probably benign	0.01
R6007:Vmn2r107	UTSW	17	20375054	missense	probably benign	0.19
R6238:Vmn2r107	UTSW	17	20345587	missense	probably benign	0.43
R6298:Vmn2r107	UTSW	17	20355782	missense	probably benign	0.00
R6467:Vmn2r107	UTSW	17	20375677	missense	probably damaging	0.99
R6726:Vmn2r107	UTSW	17	20375375	missense	probably damaging	0.96
R6782:Vmn2r107	UTSW	17	20356879	missense	probably damaging	1.00
R7299:Vmn2r107	UTSW	17	20345616	missense	probably benign	0.01
R7301:Vmn2r107	UTSW	17	20345616	missense	probably benign	0.01
R7375:Vmn2r107	UTSW	17	20355876	missense	probably benign
R7448:Vmn2r107	UTSW	17	20375732	missense	probably benign	0.00
R7495:Vmn2r107	UTSW	17	20375009	missense	possibly damaging	0.71
R7589:Vmn2r107	UTSW	17	20375372	missense	probably benign	0.05
R7594:Vmn2r107	UTSW	17	20360373	missense	probably benign	0.03
R7678:Vmn2r107	UTSW	17	20356639	missense	probably benign	0.01
R7929:Vmn2r107	UTSW	17	20345444	missense	probably null	0.96
R7974:Vmn2r107	UTSW	17	20357008	missense	probably benign	0.00
R8040:Vmn2r107	UTSW	17	20375546	missense	probably damaging	1.00
R8263:Vmn2r107	UTSW	17	20360352	missense	probably damaging	1.00
R8426:Vmn2r107	UTSW	17	20356977	missense	possibly damaging	0.91
R9175:Vmn2r107	UTSW	17	20356789	missense	possibly damaging	0.79
R9537:Vmn2r107	UTSW	17	20374887	missense	probably benign	0.00
R9642:Vmn2r107	UTSW	17	20360399	missense	probably damaging	1.00
R9711:Vmn2r107	UTSW	17	20357000	missense	probably damaging	1.00
X0022:Vmn2r107	UTSW	17	20356968	missense	possibly damaging	0.85

Predicted Primers

PCR Primer
(F):5'- CTCTAGCCAGCATAGCTTTGTG -3'
(R):5'- GGTGGAGATATTGCCATCCATATTC -3'

Sequencing Primer
(F):5'- GCACTAACTGCCTTTGTTATTGG -3'
(R):5'- CCATATTCCACAAAGAAGAAGTTGG -3'

Posted On

2016-11-08