Mutagenetix > Incidental Mutation

Incidental Mutation 'R5642:Vps13d'

440736

Institutional Source

Beutler Lab

Gene Symbol

Vps13d

Ensembl Gene

ENSMUSG00000020220

Gene Name

vacuolar protein sorting 13D

Synonyms

MMRRC Submission

043290-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5642 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

144699192-144921575 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 144896872 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glutamic Acid at position 343 (D343E)

Ref Sequence

ENSEMBL: ENSMUSP00000020441 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000020441] [ENSMUST00000036579]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000020441
AA Change: D343E

PolyPhen 2 Score 0.658 (Sensitivity: 0.87; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000020441
Gene: ENSMUSG00000020220
AA Change: D343E

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	118	1.8e-37	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
coiled coil region	665	685	N/A	INTRINSIC
low complexity region	765	781	N/A	INTRINSIC
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2866	2884	N/A	INTRINSIC
low complexity region	2973	2983	N/A	INTRINSIC
Pfam:DUF1162	3246	3530	1.1e-110	PFAM
low complexity region	3797	3810	N/A	INTRINSIC
low complexity region	3913	3921	N/A	INTRINSIC
low complexity region	4119	4132	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000036579
AA Change: D349E

PolyPhen 2 Score 0.144 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000043240
Gene: ENSMUSG00000020220
AA Change: D349E

Domain	Start	End	E-Value	Type
Pfam:Chorein_N	2	116	3.5e-35	PFAM
Pfam:VPS13	131	353	9.6e-57	PFAM
low complexity region	407	423	N/A	INTRINSIC
low complexity region	534	555	N/A	INTRINSIC
Pfam:VPS13_mid_rpt	608	896	4.3e-35	PFAM
low complexity region	1316	1329	N/A	INTRINSIC
low complexity region	1590	1603	N/A	INTRINSIC
Blast:IL1	1605	1726	2e-6	BLAST
low complexity region	1868	1883	N/A	INTRINSIC
low complexity region	2128	2141	N/A	INTRINSIC
UBA	2632	2669	3.73e-5	SMART
low complexity region	2674	2684	N/A	INTRINSIC
low complexity region	2707	2718	N/A	INTRINSIC
low complexity region	2891	2909	N/A	INTRINSIC
low complexity region	2998	3008	N/A	INTRINSIC
Pfam:SHR-BD	3271	3555	4.2e-86	PFAM
low complexity region	3822	3835	N/A	INTRINSIC
low complexity region	3938	3946	N/A	INTRINSIC
Pfam:VPS13_C	3978	4126	4.8e-24	PFAM
low complexity region	4144	4157	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein belonging to the vacuolar-protein-sorting-13 gene family. In yeast, vacuolar-protein-sorting-13 proteins are involved in trafficking of membrane proteins between the trans-Golgi network and the prevacuolar compartment. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode distinct isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a transgenic gene disruption exhibit embryonic lethality at E7. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc1	A	G	16: 14,261,319 (GRCm39)	E699G	probably damaging	Het
Ap4e1	T	A	2: 126,906,899 (GRCm39)	V1053D	possibly damaging	Het
Apobec1	T	A	6: 122,558,456 (GRCm39)	I100F	probably damaging	Het
Atp6v1g3	G	T	1: 138,211,480 (GRCm39)	K53N	probably damaging	Het
Bag3	C	T	7: 128,147,830 (GRCm39)	R482W	probably damaging	Het
Brme1	C	T	8: 84,894,114 (GRCm39)	T427I	probably damaging	Het
Cacna1i	A	G	15: 80,279,279 (GRCm39)	T2007A	possibly damaging	Het
Cd44	T	C	2: 102,731,687 (GRCm39)	D2G	probably damaging	Het
Cdadc1	T	A	14: 59,827,372 (GRCm39)	I100F	possibly damaging	Het
Cdh16	A	G	8: 105,344,677 (GRCm39)	F485L	probably damaging	Het
Cdhr5	T	A	7: 140,849,110 (GRCm39)	K817*	probably null	Het
Cfap46	G	T	7: 139,258,493 (GRCm39)	P260Q	probably damaging	Het
Clec11a	C	T	7: 43,955,832 (GRCm39)	E72K	possibly damaging	Het
Cnr2	C	A	4: 135,644,076 (GRCm39)	N51K	probably damaging	Het
Col3a1	T	A	1: 45,370,872 (GRCm39)		probably benign	Het
Cxcr1	G	A	1: 74,230,987 (GRCm39)	T345M	probably damaging	Het
Cyp2s1	T	C	7: 25,515,744 (GRCm39)		probably null	Het
Dalrd3	C	T	9: 108,449,489 (GRCm39)	T474M	probably damaging	Het
Ddit4	C	T	10: 59,787,327 (GRCm39)	S3N	probably benign	Het
Ddx41	T	C	13: 55,683,708 (GRCm39)	K108E	possibly damaging	Het
Dnaaf10	A	G	11: 17,177,263 (GRCm39)	N207S	possibly damaging	Het
Ece2	A	T	16: 20,462,477 (GRCm39)	H732L	probably benign	Het
Etv3	T	G	3: 87,443,322 (GRCm39)	L302R	possibly damaging	Het
Fam135b	A	G	15: 71,333,985 (GRCm39)	S1070P	probably damaging	Het
Fhip1b	A	T	7: 105,039,089 (GRCm39)	I50N	probably damaging	Het
Gm11567	T	A	11: 99,770,437 (GRCm39)	I125N	unknown	Het
Grm3	A	G	5: 9,620,536 (GRCm39)	L236P	probably benign	Het
Hip1	T	A	5: 135,461,939 (GRCm39)	R97*	probably null	Het
Hoxa5	T	C	6: 52,181,197 (GRCm39)	Y45C	probably damaging	Het
Ighg1	T	A	12: 113,292,654 (GRCm39)	H305L	probably damaging	Het
Inpp5b	T	A	4: 124,676,229 (GRCm39)	C362S	probably benign	Het
Kif1c	A	G	11: 70,599,273 (GRCm39)	K391E	probably benign	Het
Klf9	T	C	19: 23,119,246 (GRCm39)	V43A	probably benign	Het
Krt28	T	A	11: 99,265,320 (GRCm39)	I116F	probably damaging	Het
Lct	A	T	1: 128,222,969 (GRCm39)	D1439E	probably damaging	Het
Liph	G	A	16: 21,784,745 (GRCm39)	T284M	possibly damaging	Het
Lrrc75b	T	C	10: 75,393,055 (GRCm39)	K98R	possibly damaging	Het
Lypd4	T	C	7: 24,564,604 (GRCm39)	Q178R	probably benign	Het
Map1a	T	A	2: 121,136,524 (GRCm39)	S2209T	probably damaging	Het
Map3k21	C	T	8: 126,665,563 (GRCm39)	T584I	probably benign	Het
Map3k6	T	C	4: 132,972,855 (GRCm39)	V338A	probably damaging	Het
Mapk8ip3	A	T	17: 25,122,285 (GRCm39)	V699E	possibly damaging	Het
Mtarc1	A	C	1: 184,543,116 (GRCm39)	S71A	probably damaging	Het
Nckap1l	T	C	15: 103,363,452 (GRCm39)	S53P	probably benign	Het
Nt5e	T	C	9: 88,209,740 (GRCm39)	M1T	probably null	Het
Nudt22	T	C	19: 6,972,896 (GRCm39)	H64R	probably damaging	Het
Or1o11	A	T	17: 37,756,663 (GRCm39)	T84S	probably damaging	Het
Or2t44	A	T	11: 58,677,654 (GRCm39)	Y198F	probably damaging	Het
Or2w3	T	C	11: 58,557,225 (GRCm39)	I280T	possibly damaging	Het
Or52b1	A	T	7: 104,978,772 (GRCm39)	V209D	probably damaging	Het
Or5ac16	A	G	16: 59,022,369 (GRCm39)	L140P	probably damaging	Het
Or8k17	T	C	2: 86,066,276 (GRCm39)	N294S	probably damaging	Het
Otogl	T	C	10: 107,722,413 (GRCm39)	I314V	probably benign	Het
Pan2	G	T	10: 128,143,969 (GRCm39)	E106D	probably benign	Het
Papss1	T	A	3: 131,337,565 (GRCm39)	Y554*	probably null	Het
Pcnx1	T	C	12: 81,941,803 (GRCm39)	V67A	possibly damaging	Het
Pdpk1	A	C	17: 24,325,829 (GRCm39)	Y122*	probably null	Het
Pkd1l1	A	T	11: 8,829,202 (GRCm39)	N1463K	probably damaging	Het
Ptgfrn	C	A	3: 100,950,678 (GRCm39)	M878I	probably damaging	Het
Ranbp3	G	A	17: 57,017,703 (GRCm39)	G453E	probably benign	Het
Rapgef2	T	C	3: 79,002,157 (GRCm39)	D261G	probably damaging	Het
Reep2	A	G	18: 34,979,271 (GRCm39)	S199G	probably benign	Het
Rnpep	G	A	1: 135,205,259 (GRCm39)	T202I	probably damaging	Het
Sass6	T	A	3: 116,401,145 (GRCm39)		probably null	Het
Sema3e	A	G	5: 14,212,257 (GRCm39)	D111G	probably damaging	Het
Slc29a4	A	G	5: 142,697,727 (GRCm39)	E60G	probably damaging	Het
Spata31g1	T	C	4: 42,971,831 (GRCm39)	L388P	possibly damaging	Het
Spata31h1	T	A	10: 82,120,317 (GRCm39)	Q4231L	probably damaging	Het
Sptlc3	T	C	2: 139,388,328 (GRCm39)	Y107H	probably damaging	Het
Stx6	T	C	1: 155,073,925 (GRCm39)	I245T	probably benign	Het
Syne2	T	C	12: 75,965,306 (GRCm39)	S774P	probably damaging	Het
Tbc1d16	T	A	11: 119,049,617 (GRCm39)	Q293L	probably damaging	Het
Tbc1d30	T	C	10: 121,132,692 (GRCm39)	D224G	probably damaging	Het
Tbc1d32	T	A	10: 56,026,973 (GRCm39)	N759Y	possibly damaging	Het
Tdrd12	G	T	7: 35,210,725 (GRCm39)	A166E	probably damaging	Het
Tex14	A	G	11: 87,405,046 (GRCm39)	R653G	probably benign	Het
Them6	A	T	15: 74,593,654 (GRCm39)	R171W	probably null	Het
Tln2	T	C	9: 67,203,640 (GRCm39)	T489A	probably benign	Het
Tmem87a	A	G	2: 120,234,427 (GRCm39)	F39L	probably benign	Het
Toporsl	T	A	4: 52,611,515 (GRCm39)	C469*	probably null	Het
Tpr	T	C	1: 150,299,569 (GRCm39)	S1147P	probably damaging	Het
Trp53bp1	T	C	2: 121,067,143 (GRCm39)	M528V	probably benign	Het
Trpm6	T	A	19: 18,807,571 (GRCm39)	C1039S	probably damaging	Het
Ttc16	T	A	2: 32,665,348 (GRCm39)	S5C	probably damaging	Het
Ttn	T	C	2: 76,617,412 (GRCm39)	Y14607C	probably damaging	Het
Usp6nl	T	C	2: 6,435,275 (GRCm39)	F345L	probably damaging	Het
Vmn1r205	C	T	13: 22,776,206 (GRCm39)	G299R	probably benign	Het
Vmn2r74	G	A	7: 85,606,588 (GRCm39)	H253Y	probably benign	Het
Vwf	A	G	6: 125,580,381 (GRCm39)	E543G		Het
Zfat	A	T	15: 68,052,765 (GRCm39)	V343E	probably damaging	Het
Zfp316	T	C	5: 143,249,846 (GRCm39)	E139G	unknown	Het
Zfp943	T	A	17: 22,211,813 (GRCm39)	C300S	probably damaging	Het
Zkscan16	A	G	4: 58,957,748 (GRCm39)	K677E	probably benign	Het

Other mutations in Vps13d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Vps13d	APN	4	144,895,110 (GRCm39)	missense	probably damaging	0.98
IGL00484:Vps13d	APN	4	144,853,145 (GRCm39)	missense	probably benign	0.04
IGL00591:Vps13d	APN	4	144,917,129 (GRCm39)	missense	possibly damaging	0.95
IGL00816:Vps13d	APN	4	144,882,564 (GRCm39)	missense	probably benign	0.00
IGL00835:Vps13d	APN	4	144,887,222 (GRCm39)	missense	probably damaging	0.97
IGL00847:Vps13d	APN	4	144,811,978 (GRCm39)	missense	probably benign	0.26
IGL01084:Vps13d	APN	4	144,881,525 (GRCm39)	missense	probably benign	0.00
IGL01116:Vps13d	APN	4	144,699,320 (GRCm39)	unclassified	probably benign
IGL01150:Vps13d	APN	4	144,875,845 (GRCm39)	missense	probably benign
IGL01329:Vps13d	APN	4	144,882,776 (GRCm39)	missense	possibly damaging	0.69
IGL01338:Vps13d	APN	4	144,814,892 (GRCm39)	missense	probably damaging	1.00
IGL01583:Vps13d	APN	4	144,771,658 (GRCm39)	missense	probably damaging	1.00
IGL01598:Vps13d	APN	4	144,743,471 (GRCm39)	missense	probably benign	0.21
IGL01620:Vps13d	APN	4	144,821,437 (GRCm39)	missense	possibly damaging	0.70
IGL01636:Vps13d	APN	4	144,801,618 (GRCm39)	missense	probably damaging	1.00
IGL01723:Vps13d	APN	4	144,899,715 (GRCm39)	missense	possibly damaging	0.84
IGL01895:Vps13d	APN	4	144,882,836 (GRCm39)	missense	possibly damaging	0.57
IGL01981:Vps13d	APN	4	144,813,317 (GRCm39)	missense	probably damaging	0.99
IGL02192:Vps13d	APN	4	144,875,428 (GRCm39)	missense	probably benign	0.02
IGL02197:Vps13d	APN	4	144,854,879 (GRCm39)	missense	probably benign	0.01
IGL02209:Vps13d	APN	4	144,882,671 (GRCm39)	missense	probably damaging	0.97
IGL02219:Vps13d	APN	4	144,894,716 (GRCm39)	missense	probably benign	0.00
IGL02377:Vps13d	APN	4	144,882,934 (GRCm39)	missense	probably damaging	1.00
IGL02404:Vps13d	APN	4	144,875,305 (GRCm39)	missense	probably damaging	1.00
IGL02552:Vps13d	APN	4	144,899,707 (GRCm39)	missense	possibly damaging	0.46
IGL02651:Vps13d	APN	4	144,891,129 (GRCm39)	missense	probably benign	0.02
IGL02708:Vps13d	APN	4	144,854,850 (GRCm39)	missense	probably benign	0.12
IGL02811:Vps13d	APN	4	144,858,335 (GRCm39)	missense	possibly damaging	0.55
IGL02821:Vps13d	APN	4	144,875,332 (GRCm39)	missense	probably damaging	0.98
IGL02838:Vps13d	APN	4	144,801,595 (GRCm39)	missense	probably benign	0.31
IGL02968:Vps13d	APN	4	144,849,068 (GRCm39)	missense	probably benign	0.32
IGL03176:Vps13d	APN	4	144,801,533 (GRCm39)	missense	probably benign	0.16
IGL03352:Vps13d	APN	4	144,894,072 (GRCm39)	missense	possibly damaging	0.49
IGL03374:Vps13d	APN	4	144,835,145 (GRCm39)	missense	possibly damaging	0.70
IGL03375:Vps13d	APN	4	144,818,517 (GRCm39)	missense	probably damaging	1.00
IGL03383:Vps13d	APN	4	144,894,889 (GRCm39)	critical splice acceptor site	probably null
IGL03411:Vps13d	APN	4	144,875,894 (GRCm39)	missense	probably damaging	1.00
broken	UTSW	4	144,813,305 (GRCm39)	missense
demotion	UTSW	4	144,865,183 (GRCm39)	missense
BB008:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
BB018:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
PIT4283001:Vps13d	UTSW	4	144,835,158 (GRCm39)	missense
PIT4434001:Vps13d	UTSW	4	144,881,817 (GRCm39)	missense
R0069:Vps13d	UTSW	4	144,789,133 (GRCm39)	missense	probably benign	0.09
R0069:Vps13d	UTSW	4	144,789,133 (GRCm39)	missense	probably benign	0.09
R0076:Vps13d	UTSW	4	144,891,264 (GRCm39)	splice site	probably benign
R0211:Vps13d	UTSW	4	144,841,348 (GRCm39)	missense	probably benign	0.08
R0219:Vps13d	UTSW	4	144,832,479 (GRCm39)	missense	probably benign	0.01
R0284:Vps13d	UTSW	4	144,871,372 (GRCm39)	missense	probably benign	0.01
R0345:Vps13d	UTSW	4	144,844,195 (GRCm39)	missense	possibly damaging	0.81
R0400:Vps13d	UTSW	4	144,792,397 (GRCm39)	missense	probably benign	0.00
R0417:Vps13d	UTSW	4	144,703,130 (GRCm39)	missense	probably benign	0.19
R0538:Vps13d	UTSW	4	144,771,665 (GRCm39)	missense	probably damaging	1.00
R0560:Vps13d	UTSW	4	144,780,760 (GRCm39)	missense	probably damaging	1.00
R0627:Vps13d	UTSW	4	144,813,754 (GRCm39)	missense	probably damaging	1.00
R0707:Vps13d	UTSW	4	144,882,502 (GRCm39)	missense	probably damaging	1.00
R0782:Vps13d	UTSW	4	144,853,195 (GRCm39)	splice site	probably benign
R0925:Vps13d	UTSW	4	144,883,121 (GRCm39)	missense	probably damaging	1.00
R0993:Vps13d	UTSW	4	144,844,262 (GRCm39)	nonsense	probably null
R1135:Vps13d	UTSW	4	144,882,159 (GRCm39)	missense	probably benign	0.01
R1165:Vps13d	UTSW	4	144,853,041 (GRCm39)	missense	probably benign
R1263:Vps13d	UTSW	4	144,896,918 (GRCm39)	missense	probably benign	0.01
R1397:Vps13d	UTSW	4	144,867,904 (GRCm39)	missense	probably damaging	1.00
R1398:Vps13d	UTSW	4	144,826,553 (GRCm39)	missense	probably null
R1521:Vps13d	UTSW	4	144,832,431 (GRCm39)	missense	probably benign	0.00
R1522:Vps13d	UTSW	4	144,824,742 (GRCm39)	splice site	probably null
R1725:Vps13d	UTSW	4	144,869,830 (GRCm39)	missense	possibly damaging	0.90
R1759:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R1826:Vps13d	UTSW	4	144,881,573 (GRCm39)	missense	probably damaging	0.96
R1900:Vps13d	UTSW	4	144,853,176 (GRCm39)	missense	probably benign	0.23
R1943:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R1955:Vps13d	UTSW	4	144,882,713 (GRCm39)	missense	probably damaging	1.00
R2008:Vps13d	UTSW	4	144,881,813 (GRCm39)	missense	probably benign	0.00
R2013:Vps13d	UTSW	4	144,835,078 (GRCm39)	missense	probably damaging	0.99
R2014:Vps13d	UTSW	4	144,835,078 (GRCm39)	missense	probably damaging	0.99
R2038:Vps13d	UTSW	4	144,907,685 (GRCm39)	critical splice donor site	probably null
R2108:Vps13d	UTSW	4	144,801,617 (GRCm39)	missense	probably damaging	0.99
R2130:Vps13d	UTSW	4	144,882,671 (GRCm39)	missense	probably benign	0.17
R2134:Vps13d	UTSW	4	144,874,909 (GRCm39)	missense	probably benign	0.00
R2168:Vps13d	UTSW	4	144,813,893 (GRCm39)	splice site	probably benign
R2220:Vps13d	UTSW	4	144,904,890 (GRCm39)	missense	probably damaging	1.00
R2240:Vps13d	UTSW	4	144,837,465 (GRCm39)	missense	possibly damaging	0.70
R2332:Vps13d	UTSW	4	144,875,256 (GRCm39)	missense	probably benign
R2357:Vps13d	UTSW	4	144,801,547 (GRCm39)	frame shift	probably null
R2365:Vps13d	UTSW	4	144,813,894 (GRCm39)	splice site	probably benign
R2571:Vps13d	UTSW	4	144,875,706 (GRCm39)	missense	probably benign	0.20
R3149:Vps13d	UTSW	4	144,853,147 (GRCm39)	missense	possibly damaging	0.70
R3150:Vps13d	UTSW	4	144,813,360 (GRCm39)	missense	probably damaging	0.98
R3547:Vps13d	UTSW	4	144,801,545 (GRCm39)	missense	probably damaging	0.99
R3716:Vps13d	UTSW	4	144,802,296 (GRCm39)	missense	probably damaging	1.00
R3718:Vps13d	UTSW	4	144,802,296 (GRCm39)	missense	probably damaging	1.00
R3725:Vps13d	UTSW	4	144,842,218 (GRCm39)	splice site	probably benign
R3794:Vps13d	UTSW	4	144,812,007 (GRCm39)	splice site	probably benign
R3875:Vps13d	UTSW	4	144,917,114 (GRCm39)	missense	probably damaging	1.00
R3948:Vps13d	UTSW	4	144,867,910 (GRCm39)	missense	probably damaging	1.00
R3953:Vps13d	UTSW	4	144,875,450 (GRCm39)	missense	probably damaging	1.00
R4021:Vps13d	UTSW	4	144,801,631 (GRCm39)	missense	possibly damaging	0.90
R4323:Vps13d	UTSW	4	144,879,348 (GRCm39)	missense	probably benign	0.28
R4346:Vps13d	UTSW	4	144,799,099 (GRCm39)	intron	probably benign
R4509:Vps13d	UTSW	4	144,789,172 (GRCm39)	missense	probably damaging	1.00
R4613:Vps13d	UTSW	4	144,858,225 (GRCm39)	missense	possibly damaging	0.95
R4657:Vps13d	UTSW	4	144,801,412 (GRCm39)	missense	probably damaging	1.00
R4680:Vps13d	UTSW	4	144,835,080 (GRCm39)	missense	possibly damaging	0.94
R4688:Vps13d	UTSW	4	144,904,782 (GRCm39)	missense	probably benign
R4797:Vps13d	UTSW	4	144,780,725 (GRCm39)	missense	probably damaging	1.00
R4798:Vps13d	UTSW	4	144,904,626 (GRCm39)	missense	probably damaging	0.98
R4817:Vps13d	UTSW	4	144,795,735 (GRCm39)	missense	probably damaging	1.00
R4839:Vps13d	UTSW	4	144,812,000 (GRCm39)	missense	possibly damaging	0.95
R4860:Vps13d	UTSW	4	144,813,731 (GRCm39)	missense	probably benign
R4860:Vps13d	UTSW	4	144,813,731 (GRCm39)	missense	probably benign
R4869:Vps13d	UTSW	4	144,854,612 (GRCm39)	missense	probably damaging	1.00
R4904:Vps13d	UTSW	4	144,882,015 (GRCm39)	missense	probably damaging	1.00
R4912:Vps13d	UTSW	4	144,882,427 (GRCm39)	missense	probably benign
R4916:Vps13d	UTSW	4	144,709,963 (GRCm39)	missense	probably damaging	1.00
R4976:Vps13d	UTSW	4	144,832,468 (GRCm39)	missense	possibly damaging	0.82
R5029:Vps13d	UTSW	4	144,882,852 (GRCm39)	missense	probably benign	0.02
R5049:Vps13d	UTSW	4	144,813,336 (GRCm39)	missense	probably damaging	1.00
R5077:Vps13d	UTSW	4	144,814,811 (GRCm39)	missense	probably damaging	0.98
R5119:Vps13d	UTSW	4	144,832,468 (GRCm39)	missense	possibly damaging	0.82
R5227:Vps13d	UTSW	4	144,907,777 (GRCm39)	splice site	probably null
R5291:Vps13d	UTSW	4	144,789,139 (GRCm39)	missense	probably damaging	0.99
R5344:Vps13d	UTSW	4	144,904,904 (GRCm39)	missense	probably damaging	0.98
R5348:Vps13d	UTSW	4	144,792,459 (GRCm39)	missense	probably damaging	0.99
R5478:Vps13d	UTSW	4	144,894,120 (GRCm39)	missense	probably damaging	0.99
R5632:Vps13d	UTSW	4	144,801,452 (GRCm39)	missense	probably damaging	0.99
R5712:Vps13d	UTSW	4	144,813,743 (GRCm39)	missense	probably benign	0.07
R5747:Vps13d	UTSW	4	144,894,853 (GRCm39)	missense	probably benign	0.00
R5752:Vps13d	UTSW	4	144,875,540 (GRCm39)	missense	probably benign	0.06
R5804:Vps13d	UTSW	4	144,826,640 (GRCm39)	missense	probably benign	0.03
R5917:Vps13d	UTSW	4	144,826,580 (GRCm39)	missense	probably damaging	0.96
R5932:Vps13d	UTSW	4	144,771,611 (GRCm39)	missense	possibly damaging	0.71
R5940:Vps13d	UTSW	4	144,801,545 (GRCm39)	missense	probably benign	0.09
R5978:Vps13d	UTSW	4	144,849,181 (GRCm39)	missense	probably benign
R6031:Vps13d	UTSW	4	144,895,079 (GRCm39)	missense	probably benign	0.01
R6031:Vps13d	UTSW	4	144,895,079 (GRCm39)	missense	probably benign	0.01
R6143:Vps13d	UTSW	4	144,875,135 (GRCm39)	missense	possibly damaging	0.95
R6174:Vps13d	UTSW	4	144,701,763 (GRCm39)	nonsense	probably null
R6191:Vps13d	UTSW	4	144,875,918 (GRCm39)	missense	probably damaging	1.00
R6198:Vps13d	UTSW	4	144,875,560 (GRCm39)	missense	probably benign	0.28
R6374:Vps13d	UTSW	4	144,849,251 (GRCm39)	missense	probably damaging	1.00
R6379:Vps13d	UTSW	4	144,814,828 (GRCm39)	missense	probably benign
R6388:Vps13d	UTSW	4	144,882,144 (GRCm39)	missense	probably benign	0.06
R6418:Vps13d	UTSW	4	144,818,850 (GRCm39)	missense	probably damaging	0.98
R6466:Vps13d	UTSW	4	144,784,065 (GRCm39)	missense	possibly damaging	0.47
R6602:Vps13d	UTSW	4	144,830,234 (GRCm39)	intron	probably benign
R6604:Vps13d	UTSW	4	144,907,694 (GRCm39)	missense	probably damaging	1.00
R7051:Vps13d	UTSW	4	144,889,914 (GRCm39)	missense	probably benign	0.00
R7052:Vps13d	UTSW	4	144,889,914 (GRCm39)	missense	probably benign	0.00
R7103:Vps13d	UTSW	4	144,842,062 (GRCm39)	missense
R7231:Vps13d	UTSW	4	144,784,032 (GRCm39)	missense
R7246:Vps13d	UTSW	4	144,882,620 (GRCm39)	missense
R7339:Vps13d	UTSW	4	144,847,938 (GRCm39)	missense
R7409:Vps13d	UTSW	4	144,867,824 (GRCm39)	missense
R7419:Vps13d	UTSW	4	144,842,073 (GRCm39)	missense
R7424:Vps13d	UTSW	4	144,875,317 (GRCm39)	missense
R7439:Vps13d	UTSW	4	144,832,426 (GRCm39)	missense
R7440:Vps13d	UTSW	4	144,854,981 (GRCm39)	missense
R7528:Vps13d	UTSW	4	144,818,492 (GRCm39)	missense
R7547:Vps13d	UTSW	4	144,784,108 (GRCm39)	missense
R7558:Vps13d	UTSW	4	144,881,150 (GRCm39)	missense
R7699:Vps13d	UTSW	4	144,811,975 (GRCm39)	missense
R7729:Vps13d	UTSW	4	144,801,622 (GRCm39)	missense
R7789:Vps13d	UTSW	4	144,826,635 (GRCm39)	missense
R7813:Vps13d	UTSW	4	144,904,633 (GRCm39)	nonsense	probably null
R7834:Vps13d	UTSW	4	144,835,143 (GRCm39)	missense
R7840:Vps13d	UTSW	4	144,830,246 (GRCm39)	missense
R7880:Vps13d	UTSW	4	144,907,684 (GRCm39)	critical splice donor site	probably null
R7912:Vps13d	UTSW	4	144,899,697 (GRCm39)	missense
R7915:Vps13d	UTSW	4	144,813,389 (GRCm39)	missense
R7931:Vps13d	UTSW	4	144,822,854 (GRCm39)	nonsense	probably null
R8021:Vps13d	UTSW	4	144,875,245 (GRCm39)	missense
R8048:Vps13d	UTSW	4	144,882,137 (GRCm39)	missense
R8057:Vps13d	UTSW	4	144,701,753 (GRCm39)	missense
R8063:Vps13d	UTSW	4	144,841,327 (GRCm39)	missense
R8131:Vps13d	UTSW	4	144,882,707 (GRCm39)	missense
R8190:Vps13d	UTSW	4	144,879,321 (GRCm39)	missense
R8226:Vps13d	UTSW	4	144,875,860 (GRCm39)	missense
R8241:Vps13d	UTSW	4	144,875,047 (GRCm39)	missense
R8254:Vps13d	UTSW	4	144,709,882 (GRCm39)	splice site	probably benign
R8305:Vps13d	UTSW	4	144,818,858 (GRCm39)	missense
R8415:Vps13d	UTSW	4	144,818,549 (GRCm39)	missense
R8460:Vps13d	UTSW	4	144,897,009 (GRCm39)	intron	probably benign
R8487:Vps13d	UTSW	4	144,881,817 (GRCm39)	missense	probably benign	0.11
R8543:Vps13d	UTSW	4	144,743,353 (GRCm39)	nonsense	probably null
R8679:Vps13d	UTSW	4	144,811,977 (GRCm39)	missense
R8716:Vps13d	UTSW	4	144,802,348 (GRCm39)	missense
R8749:Vps13d	UTSW	4	144,865,183 (GRCm39)	missense
R8772:Vps13d	UTSW	4	144,801,602 (GRCm39)	missense
R8788:Vps13d	UTSW	4	144,813,305 (GRCm39)	missense
R8789:Vps13d	UTSW	4	144,795,743 (GRCm39)	missense
R8836:Vps13d	UTSW	4	144,882,648 (GRCm39)	missense
R8874:Vps13d	UTSW	4	144,881,772 (GRCm39)	missense
R8918:Vps13d	UTSW	4	144,772,873 (GRCm39)	missense
R9129:Vps13d	UTSW	4	144,898,249 (GRCm39)	missense
R9220:Vps13d	UTSW	4	144,783,058 (GRCm39)	missense
R9233:Vps13d	UTSW	4	144,879,344 (GRCm39)	missense
R9234:Vps13d	UTSW	4	144,875,792 (GRCm39)	missense
R9256:Vps13d	UTSW	4	144,882,374 (GRCm39)	missense
R9350:Vps13d	UTSW	4	144,882,333 (GRCm39)	missense
R9398:Vps13d	UTSW	4	144,896,956 (GRCm39)	nonsense	probably null
R9415:Vps13d	UTSW	4	144,796,527 (GRCm39)	missense
R9438:Vps13d	UTSW	4	144,858,314 (GRCm39)	missense
R9469:Vps13d	UTSW	4	144,780,691 (GRCm39)	missense
R9487:Vps13d	UTSW	4	144,807,869 (GRCm39)	critical splice donor site	probably null
R9524:Vps13d	UTSW	4	144,822,814 (GRCm39)	missense
R9616:Vps13d	UTSW	4	144,824,701 (GRCm39)	missense
R9655:Vps13d	UTSW	4	144,813,305 (GRCm39)	missense
R9709:Vps13d	UTSW	4	144,875,915 (GRCm39)	missense
R9767:Vps13d	UTSW	4	144,879,306 (GRCm39)	missense
R9773:Vps13d	UTSW	4	144,818,619 (GRCm39)	missense
R9779:Vps13d	UTSW	4	144,798,972 (GRCm39)	missense
R9796:Vps13d	UTSW	4	144,854,505 (GRCm39)	critical splice donor site	probably null
X0021:Vps13d	UTSW	4	144,881,595 (GRCm39)	missense	probably damaging	0.99
Z1176:Vps13d	UTSW	4	144,833,637 (GRCm39)	missense
Z1177:Vps13d	UTSW	4	144,904,866 (GRCm39)	missense
Z1177:Vps13d	UTSW	4	144,881,478 (GRCm39)	missense

Predicted Primers

PCR Primer
(F):5'- TCAGTTGTTTCTTCAACCTGTGAG -3'
(R):5'- AAGGCAAGCAGTGTTCCAGG -3'

Sequencing Primer
(F):5'- TGCAATGCCAGGACTGTG -3'
(R):5'- CCAGGTTGTCTTGGATGAAGAAC -3'

Posted On

2016-11-08