Mutagenetix > Incidental Mutation

Incidental Mutation 'R5642:Tln2'

440757

Institutional Source

Beutler Lab

Gene Symbol

Tln2

Ensembl Gene

ENSMUSG00000052698

Gene Name

talin 2

Synonyms

MMRRC Submission

043290-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.185) question?

Stock #

R5642 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

67124369-67466985 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 67203640 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 489 (T489A)

Ref Sequence

ENSEMBL: ENSMUSP00000149474 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039662] [ENSMUST00000040025] [ENSMUST00000215267] [ENSMUST00000215784] [ENSMUST00000217550]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000039662
AA Change: T1575A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000035272
Gene: ENSMUSG00000052698
AA Change: T1575A

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	3.4e-59	PFAM
Pfam:I_LWEQ	661	765	1.9e-10	PFAM
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	2.6e-67	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2384	2531	2.5e-52	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000040025
AA Change: T1575A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000039633
Gene: ENSMUSG00000052698
AA Change: T1575A

Domain	Start	End	E-Value	Type
B41	84	316	1.29e-66	SMART
IRS	311	404	6.31e-17	SMART
Pfam:Talin_middle	494	655	8.5e-78	PFAM
low complexity region	674	693	N/A	INTRINSIC
internal_repeat_2	703	763	2.05e-7	PROSPERO
low complexity region	770	778	N/A	INTRINSIC
PDB:2L7A\|A	797	901	3e-44	PDB
low complexity region	902	918	N/A	INTRINSIC
low complexity region	923	946	N/A	INTRINSIC
internal_repeat_4	973	1033	7.18e-6	PROSPERO
internal_repeat_3	1083	1210	3.53e-6	PROSPERO
internal_repeat_4	1138	1198	7.18e-6	PROSPERO
low complexity region	1313	1325	N/A	INTRINSIC
low complexity region	1330	1343	N/A	INTRINSIC
internal_repeat_1	1482	1554	2.88e-9	PROSPERO
internal_repeat_2	1491	1551	2.05e-7	PROSPERO
low complexity region	1679	1690	N/A	INTRINSIC
Pfam:VBS	1850	1974	9.9e-72	PFAM
internal_repeat_3	2010	2138	3.53e-6	PROSPERO
low complexity region	2309	2325	N/A	INTRINSIC
low complexity region	2349	2359	N/A	INTRINSIC
Pfam:I_LWEQ	2383	2533	4.3e-51	PFAM

Predicted Effect

unknown
Transcript: ENSMUST00000214859
AA Change: T29A

Predicted Effect

probably benign
Transcript: ENSMUST00000215267
AA Change: T485A

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

Predicted Effect

probably benign
Transcript: ENSMUST00000215784
AA Change: T1577A

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

Predicted Effect

probably benign
Transcript: ENSMUST00000217550
AA Change: T489A

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.1%
20x: 94.8%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein related to talin 1, a cytoskeletal protein that plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit abnormal muscle morphology. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 93 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcc1	A	G	16: 14,261,319 (GRCm39)	E699G	probably damaging	Het
Ap4e1	T	A	2: 126,906,899 (GRCm39)	V1053D	possibly damaging	Het
Apobec1	T	A	6: 122,558,456 (GRCm39)	I100F	probably damaging	Het
Atp6v1g3	G	T	1: 138,211,480 (GRCm39)	K53N	probably damaging	Het
Bag3	C	T	7: 128,147,830 (GRCm39)	R482W	probably damaging	Het
Brme1	C	T	8: 84,894,114 (GRCm39)	T427I	probably damaging	Het
Cacna1i	A	G	15: 80,279,279 (GRCm39)	T2007A	possibly damaging	Het
Cd44	T	C	2: 102,731,687 (GRCm39)	D2G	probably damaging	Het
Cdadc1	T	A	14: 59,827,372 (GRCm39)	I100F	possibly damaging	Het
Cdh16	A	G	8: 105,344,677 (GRCm39)	F485L	probably damaging	Het
Cdhr5	T	A	7: 140,849,110 (GRCm39)	K817*	probably null	Het
Cfap46	G	T	7: 139,258,493 (GRCm39)	P260Q	probably damaging	Het
Clec11a	C	T	7: 43,955,832 (GRCm39)	E72K	possibly damaging	Het
Cnr2	C	A	4: 135,644,076 (GRCm39)	N51K	probably damaging	Het
Col3a1	T	A	1: 45,370,872 (GRCm39)		probably benign	Het
Cxcr1	G	A	1: 74,230,987 (GRCm39)	T345M	probably damaging	Het
Cyp2s1	T	C	7: 25,515,744 (GRCm39)		probably null	Het
Dalrd3	C	T	9: 108,449,489 (GRCm39)	T474M	probably damaging	Het
Ddit4	C	T	10: 59,787,327 (GRCm39)	S3N	probably benign	Het
Ddx41	T	C	13: 55,683,708 (GRCm39)	K108E	possibly damaging	Het
Dnaaf10	A	G	11: 17,177,263 (GRCm39)	N207S	possibly damaging	Het
Ece2	A	T	16: 20,462,477 (GRCm39)	H732L	probably benign	Het
Etv3	T	G	3: 87,443,322 (GRCm39)	L302R	possibly damaging	Het
Fam135b	A	G	15: 71,333,985 (GRCm39)	S1070P	probably damaging	Het
Fhip1b	A	T	7: 105,039,089 (GRCm39)	I50N	probably damaging	Het
Gm11567	T	A	11: 99,770,437 (GRCm39)	I125N	unknown	Het
Grm3	A	G	5: 9,620,536 (GRCm39)	L236P	probably benign	Het
Hip1	T	A	5: 135,461,939 (GRCm39)	R97*	probably null	Het
Hoxa5	T	C	6: 52,181,197 (GRCm39)	Y45C	probably damaging	Het
Ighg1	T	A	12: 113,292,654 (GRCm39)	H305L	probably damaging	Het
Inpp5b	T	A	4: 124,676,229 (GRCm39)	C362S	probably benign	Het
Kif1c	A	G	11: 70,599,273 (GRCm39)	K391E	probably benign	Het
Klf9	T	C	19: 23,119,246 (GRCm39)	V43A	probably benign	Het
Krt28	T	A	11: 99,265,320 (GRCm39)	I116F	probably damaging	Het
Lct	A	T	1: 128,222,969 (GRCm39)	D1439E	probably damaging	Het
Liph	G	A	16: 21,784,745 (GRCm39)	T284M	possibly damaging	Het
Lrrc75b	T	C	10: 75,393,055 (GRCm39)	K98R	possibly damaging	Het
Lypd4	T	C	7: 24,564,604 (GRCm39)	Q178R	probably benign	Het
Map1a	T	A	2: 121,136,524 (GRCm39)	S2209T	probably damaging	Het
Map3k21	C	T	8: 126,665,563 (GRCm39)	T584I	probably benign	Het
Map3k6	T	C	4: 132,972,855 (GRCm39)	V338A	probably damaging	Het
Mapk8ip3	A	T	17: 25,122,285 (GRCm39)	V699E	possibly damaging	Het
Mtarc1	A	C	1: 184,543,116 (GRCm39)	S71A	probably damaging	Het
Nckap1l	T	C	15: 103,363,452 (GRCm39)	S53P	probably benign	Het
Nt5e	T	C	9: 88,209,740 (GRCm39)	M1T	probably null	Het
Nudt22	T	C	19: 6,972,896 (GRCm39)	H64R	probably damaging	Het
Or1o11	A	T	17: 37,756,663 (GRCm39)	T84S	probably damaging	Het
Or2t44	A	T	11: 58,677,654 (GRCm39)	Y198F	probably damaging	Het
Or2w3	T	C	11: 58,557,225 (GRCm39)	I280T	possibly damaging	Het
Or52b1	A	T	7: 104,978,772 (GRCm39)	V209D	probably damaging	Het
Or5ac16	A	G	16: 59,022,369 (GRCm39)	L140P	probably damaging	Het
Or8k17	T	C	2: 86,066,276 (GRCm39)	N294S	probably damaging	Het
Otogl	T	C	10: 107,722,413 (GRCm39)	I314V	probably benign	Het
Pan2	G	T	10: 128,143,969 (GRCm39)	E106D	probably benign	Het
Papss1	T	A	3: 131,337,565 (GRCm39)	Y554*	probably null	Het
Pcnx1	T	C	12: 81,941,803 (GRCm39)	V67A	possibly damaging	Het
Pdpk1	A	C	17: 24,325,829 (GRCm39)	Y122*	probably null	Het
Pkd1l1	A	T	11: 8,829,202 (GRCm39)	N1463K	probably damaging	Het
Ptgfrn	C	A	3: 100,950,678 (GRCm39)	M878I	probably damaging	Het
Ranbp3	G	A	17: 57,017,703 (GRCm39)	G453E	probably benign	Het
Rapgef2	T	C	3: 79,002,157 (GRCm39)	D261G	probably damaging	Het
Reep2	A	G	18: 34,979,271 (GRCm39)	S199G	probably benign	Het
Rnpep	G	A	1: 135,205,259 (GRCm39)	T202I	probably damaging	Het
Sass6	T	A	3: 116,401,145 (GRCm39)		probably null	Het
Sema3e	A	G	5: 14,212,257 (GRCm39)	D111G	probably damaging	Het
Slc29a4	A	G	5: 142,697,727 (GRCm39)	E60G	probably damaging	Het
Spata31g1	T	C	4: 42,971,831 (GRCm39)	L388P	possibly damaging	Het
Spata31h1	T	A	10: 82,120,317 (GRCm39)	Q4231L	probably damaging	Het
Sptlc3	T	C	2: 139,388,328 (GRCm39)	Y107H	probably damaging	Het
Stx6	T	C	1: 155,073,925 (GRCm39)	I245T	probably benign	Het
Syne2	T	C	12: 75,965,306 (GRCm39)	S774P	probably damaging	Het
Tbc1d16	T	A	11: 119,049,617 (GRCm39)	Q293L	probably damaging	Het
Tbc1d30	T	C	10: 121,132,692 (GRCm39)	D224G	probably damaging	Het
Tbc1d32	T	A	10: 56,026,973 (GRCm39)	N759Y	possibly damaging	Het
Tdrd12	G	T	7: 35,210,725 (GRCm39)	A166E	probably damaging	Het
Tex14	A	G	11: 87,405,046 (GRCm39)	R653G	probably benign	Het
Them6	A	T	15: 74,593,654 (GRCm39)	R171W	probably null	Het
Tmem87a	A	G	2: 120,234,427 (GRCm39)	F39L	probably benign	Het
Toporsl	T	A	4: 52,611,515 (GRCm39)	C469*	probably null	Het
Tpr	T	C	1: 150,299,569 (GRCm39)	S1147P	probably damaging	Het
Trp53bp1	T	C	2: 121,067,143 (GRCm39)	M528V	probably benign	Het
Trpm6	T	A	19: 18,807,571 (GRCm39)	C1039S	probably damaging	Het
Ttc16	T	A	2: 32,665,348 (GRCm39)	S5C	probably damaging	Het
Ttn	T	C	2: 76,617,412 (GRCm39)	Y14607C	probably damaging	Het
Usp6nl	T	C	2: 6,435,275 (GRCm39)	F345L	probably damaging	Het
Vmn1r205	C	T	13: 22,776,206 (GRCm39)	G299R	probably benign	Het
Vmn2r74	G	A	7: 85,606,588 (GRCm39)	H253Y	probably benign	Het
Vps13d	A	T	4: 144,896,872 (GRCm39)	D343E	possibly damaging	Het
Vwf	A	G	6: 125,580,381 (GRCm39)	E543G		Het
Zfat	A	T	15: 68,052,765 (GRCm39)	V343E	probably damaging	Het
Zfp316	T	C	5: 143,249,846 (GRCm39)	E139G	unknown	Het
Zfp943	T	A	17: 22,211,813 (GRCm39)	C300S	probably damaging	Het
Zkscan16	A	G	4: 58,957,748 (GRCm39)	K677E	probably benign	Het

Other mutations in Tln2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00468:Tln2	APN	9	67,251,469 (GRCm39)	missense	possibly damaging	0.59
IGL01110:Tln2	APN	9	67,157,864 (GRCm39)	nonsense	probably null
IGL01112:Tln2	APN	9	67,219,093 (GRCm39)	missense	probably damaging	1.00
IGL01307:Tln2	APN	9	67,302,749 (GRCm39)	missense	probably benign	0.25
IGL01374:Tln2	APN	9	67,169,205 (GRCm39)	missense	probably damaging	1.00
IGL01625:Tln2	APN	9	67,277,905 (GRCm39)	missense	probably damaging	1.00
IGL01865:Tln2	APN	9	67,157,896 (GRCm39)	nonsense	probably null
IGL01999:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02002:Tln2	APN	9	67,263,980 (GRCm39)	missense	probably damaging	0.98
IGL02005:Tln2	APN	9	67,299,787 (GRCm39)	missense	possibly damaging	0.81
IGL02015:Tln2	APN	9	67,268,721 (GRCm39)	splice site	probably benign
IGL02368:Tln2	APN	9	67,148,092 (GRCm39)	splice site	probably benign
IGL02444:Tln2	APN	9	67,165,874 (GRCm39)	splice site	probably benign
IGL02646:Tln2	APN	9	67,163,278 (GRCm39)	missense	probably benign	0.43
IGL02744:Tln2	APN	9	67,136,658 (GRCm39)	nonsense	probably null
IGL02869:Tln2	APN	9	67,128,807 (GRCm39)	splice site	probably benign
IGL02930:Tln2	APN	9	67,300,944 (GRCm39)	nonsense	probably null
IGL03100:Tln2	APN	9	67,203,019 (GRCm39)	missense	probably damaging	1.00
IGL03326:Tln2	APN	9	67,241,539 (GRCm39)	missense	possibly damaging	0.67
Harrier	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
Marsh	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
BB008:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
BB018:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0047:Tln2	UTSW	9	67,147,954 (GRCm39)	splice site	probably benign
R0107:Tln2	UTSW	9	67,277,988 (GRCm39)	missense	probably damaging	1.00
R0494:Tln2	UTSW	9	67,262,479 (GRCm39)	missense	probably benign	0.22
R0884:Tln2	UTSW	9	67,278,015 (GRCm39)	missense	probably damaging	1.00
R0947:Tln2	UTSW	9	67,203,095 (GRCm39)	missense	probably benign	0.08
R0989:Tln2	UTSW	9	67,136,736 (GRCm39)	missense	probably damaging	1.00
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1195:Tln2	UTSW	9	67,165,848 (GRCm39)	missense	probably damaging	0.96
R1486:Tln2	UTSW	9	67,219,121 (GRCm39)	missense	probably damaging	1.00
R1527:Tln2	UTSW	9	67,179,950 (GRCm39)	missense	possibly damaging	0.95
R1584:Tln2	UTSW	9	67,203,696 (GRCm39)	missense	probably damaging	1.00
R1636:Tln2	UTSW	9	67,213,814 (GRCm39)	missense	probably damaging	1.00
R1656:Tln2	UTSW	9	67,134,389 (GRCm39)	missense	possibly damaging	0.81
R1707:Tln2	UTSW	9	67,283,089 (GRCm39)	missense	probably benign	0.00
R1749:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1751:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1761:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1767:Tln2	UTSW	9	67,193,796 (GRCm39)	missense	probably benign	0.01
R1815:Tln2	UTSW	9	67,136,705 (GRCm39)	missense	probably damaging	1.00
R1840:Tln2	UTSW	9	67,249,325 (GRCm39)	missense	probably damaging	1.00
R1847:Tln2	UTSW	9	67,269,969 (GRCm39)	nonsense	probably null
R1964:Tln2	UTSW	9	67,249,417 (GRCm39)	missense	probably benign	0.00
R1968:Tln2	UTSW	9	67,163,183 (GRCm39)	missense	probably damaging	1.00
R2036:Tln2	UTSW	9	67,179,986 (GRCm39)	missense	possibly damaging	0.76
R2038:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R2152:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2153:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2154:Tln2	UTSW	9	67,209,842 (GRCm39)	missense	probably damaging	1.00
R2191:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2192:Tln2	UTSW	9	67,262,503 (GRCm39)	missense	probably damaging	1.00
R2201:Tln2	UTSW	9	67,283,039 (GRCm39)	missense	probably damaging	1.00
R3116:Tln2	UTSW	9	67,262,421 (GRCm39)	missense	probably benign	0.10
R3151:Tln2	UTSW	9	67,237,829 (GRCm39)	critical splice donor site	probably null
R3795:Tln2	UTSW	9	67,163,197 (GRCm39)	missense	probably damaging	0.97
R3953:Tln2	UTSW	9	67,277,911 (GRCm39)	missense	probably damaging	1.00
R4450:Tln2	UTSW	9	67,251,347 (GRCm39)	critical splice donor site	probably null
R4685:Tln2	UTSW	9	67,209,854 (GRCm39)	missense	probably damaging	1.00
R4688:Tln2	UTSW	9	67,304,935 (GRCm39)	start codon destroyed	probably benign	0.01
R4696:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4697:Tln2	UTSW	9	67,302,743 (GRCm39)	missense	probably damaging	1.00
R4700:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4701:Tln2	UTSW	9	67,253,809 (GRCm39)	missense	probably benign	0.03
R4741:Tln2	UTSW	9	67,293,837 (GRCm39)	critical splice donor site	probably null
R4806:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4807:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4808:Tln2	UTSW	9	67,239,015 (GRCm39)	missense	probably benign	0.29
R4967:Tln2	UTSW	9	67,262,407 (GRCm39)	missense	probably damaging	0.97
R5061:Tln2	UTSW	9	67,261,750 (GRCm39)	missense	probably benign
R5092:Tln2	UTSW	9	67,163,310 (GRCm39)	missense	probably benign	0.13
R5093:Tln2	UTSW	9	67,241,596 (GRCm39)	missense	probably benign	0.44
R5126:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R5204:Tln2	UTSW	9	67,261,764 (GRCm39)	missense	probably benign	0.00
R5236:Tln2	UTSW	9	67,273,205 (GRCm39)	missense	probably damaging	0.99
R5287:Tln2	UTSW	9	67,149,641 (GRCm39)	missense	probably damaging	1.00
R5568:Tln2	UTSW	9	67,219,147 (GRCm39)	missense	probably damaging	1.00
R5571:Tln2	UTSW	9	67,241,602 (GRCm39)	missense	possibly damaging	0.88
R5711:Tln2	UTSW	9	67,299,829 (GRCm39)	missense	probably benign	0.00
R5776:Tln2	UTSW	9	67,165,532 (GRCm39)	missense	probably damaging	1.00
R5791:Tln2	UTSW	9	67,293,887 (GRCm39)	missense	probably damaging	0.98
R5866:Tln2	UTSW	9	67,174,150 (GRCm39)	missense	probably damaging	1.00
R5888:Tln2	UTSW	9	67,136,685 (GRCm39)	missense	probably damaging	1.00
R5902:Tln2	UTSW	9	67,269,999 (GRCm39)	missense	probably benign	0.02
R6106:Tln2	UTSW	9	67,230,302 (GRCm39)	missense	probably damaging	0.99
R6175:Tln2	UTSW	9	67,131,363 (GRCm39)	missense	probably damaging	1.00
R6385:Tln2	UTSW	9	67,185,411 (GRCm39)	missense	probably benign	0.45
R6430:Tln2	UTSW	9	67,179,947 (GRCm39)	missense	probably damaging	1.00
R6441:Tln2	UTSW	9	67,179,971 (GRCm39)	missense	probably damaging	1.00
R6738:Tln2	UTSW	9	67,293,946 (GRCm39)	missense	possibly damaging	0.91
R6776:Tln2	UTSW	9	67,170,187 (GRCm39)	missense	probably damaging	1.00
R6794:Tln2	UTSW	9	67,193,840 (GRCm39)	missense	probably benign	0.07
R6850:Tln2	UTSW	9	67,165,817 (GRCm39)	missense	probably damaging	1.00
R6907:Tln2	UTSW	9	67,304,917 (GRCm39)	missense	probably damaging	0.98
R6909:Tln2	UTSW	9	67,299,814 (GRCm39)	missense	probably damaging	0.97
R6951:Tln2	UTSW	9	67,165,767 (GRCm39)	missense	probably damaging	0.97
R7015:Tln2	UTSW	9	67,269,929 (GRCm39)	missense	possibly damaging	0.55
R7051:Tln2	UTSW	9	67,253,699 (GRCm39)	missense	probably benign	0.00
R7246:Tln2	UTSW	9	67,170,261 (GRCm39)	missense	probably damaging	1.00
R7292:Tln2	UTSW	9	67,253,743 (GRCm39)	missense	probably benign
R7753:Tln2	UTSW	9	67,302,755 (GRCm39)	missense	probably damaging	1.00
R7868:Tln2	UTSW	9	67,255,508 (GRCm39)	missense	probably damaging	1.00
R7931:Tln2	UTSW	9	67,165,742 (GRCm39)	critical splice donor site	probably null
R8023:Tln2	UTSW	9	67,131,346 (GRCm39)	missense	probably damaging	1.00
R8081:Tln2	UTSW	9	67,264,029 (GRCm39)	missense	probably damaging	1.00
R8164:Tln2	UTSW	9	67,226,702 (GRCm39)	missense	probably benign	0.31
R8192:Tln2	UTSW	9	67,253,811 (GRCm39)	nonsense	probably null
R8495:Tln2	UTSW	9	67,261,749 (GRCm39)	missense	probably benign	0.01
R8734:Tln2	UTSW	9	67,179,936 (GRCm39)	missense	probably benign	0.19
R8739:Tln2	UTSW	9	67,165,555 (GRCm39)	missense	probably damaging	1.00
R8757:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8759:Tln2	UTSW	9	67,274,500 (GRCm39)	missense	probably damaging	1.00
R8770:Tln2	UTSW	9	67,230,304 (GRCm39)	missense	probably benign
R8781:Tln2	UTSW	9	67,163,233 (GRCm39)	missense	probably damaging	1.00
R8812:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8814:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8816:Tln2	UTSW	9	67,128,799 (GRCm39)	missense	probably damaging	1.00
R8816:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8833:Tln2	UTSW	9	67,128,693 (GRCm39)	missense	possibly damaging	0.75
R8835:Tln2	UTSW	9	67,304,975 (GRCm39)	splice site	probably benign
R8837:Tln2	UTSW	9	67,157,866 (GRCm39)	missense	probably damaging	0.99
R8843:Tln2	UTSW	9	67,302,827 (GRCm39)	missense	probably damaging	1.00
R8864:Tln2	UTSW	9	67,237,834 (GRCm39)	nonsense	probably null
R8867:Tln2	UTSW	9	67,237,832 (GRCm39)	missense	probably damaging	0.98
R8921:Tln2	UTSW	9	67,174,105 (GRCm39)	missense	probably damaging	0.99
R9080:Tln2	UTSW	9	67,253,843 (GRCm39)	missense	probably damaging	1.00
R9083:Tln2	UTSW	9	67,269,927 (GRCm39)	missense	probably damaging	0.96
R9150:Tln2	UTSW	9	67,128,778 (GRCm39)	missense	probably damaging	1.00
R9287:Tln2	UTSW	9	67,277,980 (GRCm39)	missense	probably benign	0.20
R9330:Tln2	UTSW	9	67,229,213 (GRCm39)	missense	possibly damaging	0.61
R9343:Tln2	UTSW	9	67,230,353 (GRCm39)	missense	probably benign	0.10
R9355:Tln2	UTSW	9	67,262,529 (GRCm39)	missense	possibly damaging	0.46
R9383:Tln2	UTSW	9	67,278,043 (GRCm39)	missense	probably benign	0.17
R9386:Tln2	UTSW	9	67,273,249 (GRCm39)	missense	possibly damaging	0.78
R9407:Tln2	UTSW	9	67,136,732 (GRCm39)	missense	probably damaging	1.00
R9483:Tln2	UTSW	9	67,299,769 (GRCm39)	missense	probably damaging	1.00
R9523:Tln2	UTSW	9	67,165,766 (GRCm39)	missense	probably damaging	0.99
R9642:Tln2	UTSW	9	67,157,826 (GRCm39)	missense	probably benign	0.02
R9703:Tln2	UTSW	9	67,293,938 (GRCm39)	missense	probably damaging	1.00
X0027:Tln2	UTSW	9	67,284,135 (GRCm39)	missense	probably damaging	1.00
X0064:Tln2	UTSW	9	67,255,420 (GRCm39)	missense	probably damaging	1.00
X0067:Tln2	UTSW	9	67,277,973 (GRCm39)	missense	probably damaging	1.00
Z1176:Tln2	UTSW	9	67,253,767 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- ACTGTGCACCAAGTACCATC -3'
(R):5'- GAACTACCTTCCCTTGCTGG -3'

Sequencing Primer
(F):5'- CCATCCACAGTAACAGAGTTATGTG -3'
(R):5'- CCTTGCTGGGAGGCGATTG -3'

Posted On

2016-11-08