|Institutional Source||Beutler Lab|
|Gene Name||myosin IXb|
|Is this an essential gene?||Possibly essential (E-score: 0.718)|
|Stock #||R0103 (G1)|
|Chromosomal Location||71272714-71360713 bp(+) (GRCm38)|
|Type of Mutation||splice site|
|DNA Base Change (assembly)||C to T at 71323849 bp (GRCm38)|
|Amino Acid Change|
|Ref Sequence||ENSEMBL: ENSMUSP00000148316 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000071935] [ENSMUST00000168839] [ENSMUST00000170242] [ENSMUST00000212935]|
|AlphaFold||no structure available at present|
|Coding Region Coverage||
|Validation Efficiency||99% (84/85)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). The protein has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. The protein complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. The protein also has rho-GTPase activity. Polymorphisms in this gene are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
PHENOTYPE: Homozygous null mutants breed normal, but shows defect in macrophage motility and chemotaxis. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Myo9b||
(F):5'- TCCTCTGGAAATCAGACAGGCAGC -3'
(R):5'- TGCCTCATTTTGCAGCCAAGAAAC -3'
(F):5'- ggcatgaaggttgtatgttcaag -3'
(R):5'- TTTTGCAGCCAAGAAACAAGGG -3'