Mutagenetix > Incidental Mutation

Incidental Mutation 'R5677:Zfp850'

442793

Institutional Source

Beutler Lab

Gene Symbol

Zfp850

Ensembl Gene

ENSMUSG00000096916

Gene Name

zinc finger protein 850

Synonyms

C130069I09Rik, Gm4636

MMRRC Submission

043316-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.067) question?

Stock #

R5677 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

27984854-28014115 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 27989088 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 565 (Y565C)

Ref Sequence

ENSEMBL: ENSMUSP00000141063 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099111] [ENSMUST00000180024] [ENSMUST00000180502]

AlphaFold

J3QPC5

Predicted Effect

probably benign
Transcript: ENSMUST00000099111

Predicted Effect

probably damaging
Transcript: ENSMUST00000180024
AA Change: Y565C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000137192
Gene: ENSMUSG00000096916
AA Change: Y565C

Domain	Start	End	E-Value	Type
KRAB	14	75	1.56e-34	SMART
ZnF_C2H2	172	194	7.18e1	SMART
ZnF_C2H2	200	222	3.63e-3	SMART
ZnF_C2H2	228	250	8.94e-3	SMART
ZnF_C2H2	256	278	7.49e-5	SMART
ZnF_C2H2	313	335	1.01e-1	SMART
ZnF_C2H2	341	363	4.4e-2	SMART
ZnF_C2H2	369	391	7.37e-4	SMART
ZnF_C2H2	397	419	8.47e-4	SMART
ZnF_C2H2	425	447	1.92e-2	SMART
ZnF_C2H2	453	475	2.99e-4	SMART
ZnF_C2H2	481	503	7.78e-3	SMART
ZnF_C2H2	509	531	1.95e-3	SMART
ZnF_C2H2	537	559	1.92e-2	SMART
ZnF_C2H2	565	587	2.99e-4	SMART
ZnF_C2H2	593	615	1.79e-2	SMART
ZnF_C2H2	621	643	7.37e-4	SMART
ZnF_C2H2	649	671	4.4e-2	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000180502
AA Change: Y565C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000141063
Gene: ENSMUSG00000096916
AA Change: Y565C

Domain	Start	End	E-Value	Type
KRAB	14	75	6.5e-37	SMART
ZnF_C2H2	172	194	3e-1	SMART
ZnF_C2H2	200	222	1.5e-5	SMART
ZnF_C2H2	228	250	3.8e-5	SMART
ZnF_C2H2	256	274	2.5e-1	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057J18Rik	C	T	10: 28,986,229 (GRCm38)	V22I	probably benign	Het
4930430A15Rik	T	C	2: 111,211,565 (GRCm38)	T342A	probably benign	Het
4931423N10Rik	T	A	2: 23,212,718 (GRCm38)	L156Q	probably damaging	Het
Abca8a	A	T	11: 110,038,399 (GRCm38)	V1296D	possibly damaging	Het
Abca8b	C	T	11: 109,940,861 (GRCm38)	S1328N	probably damaging	Het
Adcy1	G	A	11: 7,161,914 (GRCm38)	M926I	probably damaging	Het
Aff4	G	T	11: 53,400,275 (GRCm38)	M687I	possibly damaging	Het
Agbl2	T	A	2: 90,807,978 (GRCm38)	Y636N	possibly damaging	Het
Agtr1a	A	T	13: 30,381,584 (GRCm38)	I211F	probably damaging	Het
Alkbh8	T	G	9: 3,385,147 (GRCm38)	S480A	possibly damaging	Het
Ankrd13b	A	T	11: 77,477,544 (GRCm38)	V84E	probably damaging	Het
Ap3b1	C	T	13: 94,528,196 (GRCm38)	T881I	unknown	Het
Apbb1	A	C	7: 105,559,246 (GRCm38)	D617E	probably damaging	Het
Apobec4	A	G	1: 152,757,282 (GRCm38)	R354G	probably benign	Het
BC003331	G	A	1: 150,374,837 (GRCm38)	L319F	probably damaging	Het
Brdt	T	G	5: 107,348,617 (GRCm38)	C198W	possibly damaging	Het
Cacna1b	G	T	2: 24,679,358 (GRCm38)	H851Q	possibly damaging	Het
Car2	G	T	3: 14,898,055 (GRCm38)	V217F	possibly damaging	Het
Ccdc24	A	C	4: 117,869,880 (GRCm38)		probably benign	Het
Chodl	C	A	16: 78,941,315 (GRCm38)	A57E	probably damaging	Het
Clgn	G	T	8: 83,409,538 (GRCm38)	C185F	probably damaging	Het
Cltc	G	T	11: 86,705,242 (GRCm38)	N1223K	probably damaging	Het
Cnot10	T	C	9: 114,629,093 (GRCm38)	N115S	probably damaging	Het
Col12a1	T	C	9: 79,699,321 (GRCm38)	R607G	probably damaging	Het
Cpd	A	G	11: 76,799,825 (GRCm38)	V835A	probably benign	Het
Csmd3	G	C	15: 48,622,051 (GRCm38)	L153V	probably damaging	Het
Ctr9	A	G	7: 111,044,002 (GRCm38)	H527R	probably benign	Het
Cwc22	G	A	2: 77,929,443 (GRCm38)	R87W	probably damaging	Het
D930020B18Rik	T	A	10: 121,669,201 (GRCm38)	N107K	probably benign	Het
Dgkg	C	A	16: 22,570,171 (GRCm38)	V418L	probably benign	Het
Dhx40	A	G	11: 86,800,963 (GRCm38)		probably null	Het
Diaph1	A	T	18: 37,855,951 (GRCm38)	M910K	probably damaging	Het
Diras2	T	A	13: 52,507,675 (GRCm38)	M199L	possibly damaging	Het
Dnah8	G	A	17: 30,748,568 (GRCm38)	D2585N	probably benign	Het
Dock5	T	A	14: 67,777,603 (GRCm38)	Q1302H	probably benign	Het
Dync1i2	T	C	2: 71,228,623 (GRCm38)	S90P	probably benign	Het
E2f8	A	T	7: 48,867,195 (GRCm38)	V812E	probably damaging	Het
Egfem1	C	A	3: 29,690,174 (GRCm38)	Q521K	probably damaging	Het
Fbxl18	A	T	5: 142,878,720 (GRCm38)	C699*	probably null	Het
Fgf3	C	T	7: 144,838,783 (GRCm38)	R26*	probably null	Het
Fpr-rs7	T	G	17: 20,114,103 (GRCm38)	I42L	probably benign	Het
Gm13101	A	T	4: 143,965,138 (GRCm38)	D338E	possibly damaging	Het
Gm3159	T	A	14: 4,398,582 (GRCm38)	M91K	probably damaging	Het
Gprin3	A	G	6: 59,353,892 (GRCm38)	S477P	possibly damaging	Het
Grm8	A	T	6: 27,761,204 (GRCm38)		probably null	Het
Hepacam2	A	T	6: 3,466,142 (GRCm38)	D420E	probably damaging	Het
Hmcn1	A	T	1: 150,609,778 (GRCm38)	W4358R	probably benign	Het
Ifna6	G	A	4: 88,827,719 (GRCm38)	A102T	probably benign	Het
Ighv2-2	T	C	12: 113,588,522 (GRCm38)	Q32R	probably benign	Het
Igkv1-131	T	A	6: 67,766,258 (GRCm38)	Q47L	possibly damaging	Het
Il16	T	C	7: 83,674,553 (GRCm38)	E263G	probably damaging	Het
Kansl1	A	T	11: 104,335,148 (GRCm38)	C981S	probably benign	Het
Lrp1	A	T	10: 127,574,429 (GRCm38)	F1483I	probably damaging	Het
Ltf	T	C	9: 111,020,912 (GRCm38)	M1T	probably null	Het
Ly75	C	T	2: 60,299,082 (GRCm38)	R1653H	probably benign	Het
Macrod2	T	C	2: 142,176,667 (GRCm38)	F240S	probably damaging	Het
Man1c1	T	C	4: 134,569,060 (GRCm38)	E433G	probably damaging	Het
Mansc4	A	T	6: 147,081,549 (GRCm38)	M130K	probably benign	Het
Mccc1	C	T	3: 35,990,048 (GRCm38)		probably null	Het
Mink1	G	T	11: 70,605,165 (GRCm38)	R75L	possibly damaging	Het
Mst1	A	G	9: 108,081,286 (GRCm38)	D65G	probably damaging	Het
Myo9b	C	T	8: 71,343,686 (GRCm38)	A857V	probably damaging	Het
Ndufa4	A	G	6: 11,900,575 (GRCm38)	V70A	probably benign	Het
Npat	T	A	9: 53,555,100 (GRCm38)	S230T	probably benign	Het
Nr1d1	A	G	11: 98,771,308 (GRCm38)	Y167H	probably damaging	Het
Oca2	A	G	7: 56,414,462 (GRCm38)	D735G	probably damaging	Het
Olfr13	T	C	6: 43,174,331 (GRCm38)	V115A	probably benign	Het
Olfr1359	C	T	13: 21,703,223 (GRCm38)	T74I	probably benign	Het
Olfr1383	A	G	11: 49,523,944 (GRCm38)	T74A	probably damaging	Het
Olfr835	T	A	9: 19,035,558 (GRCm38)	I145N	possibly damaging	Het
Otop1	A	G	5: 38,300,163 (GRCm38)	Y422C	probably damaging	Het
Pde4dip	G	A	3: 97,841,648 (GRCm38)	R126*	probably null	Het
Pdp2	C	T	8: 104,594,688 (GRCm38)	P390S	probably damaging	Het
Pds5b	A	T	5: 150,716,461 (GRCm38)	T14S	possibly damaging	Het
Pfkp	T	C	13: 6,588,595 (GRCm38)	E580G	probably damaging	Het
Piezo2	A	T	18: 63,117,696 (GRCm38)	L212Q	possibly damaging	Het
Piezo2	G	C	18: 63,117,697 (GRCm38)	L444V	probably benign	Het
Pla2g4d	T	A	2: 120,278,948 (GRCm38)	T207S	possibly damaging	Het
Plk2	C	A	13: 110,399,057 (GRCm38)	T471K	possibly damaging	Het
Ppp1r3g	G	A	13: 35,969,262 (GRCm38)	E222K	probably damaging	Het
Prkcg	A	G	7: 3,323,458 (GRCm38)	D480G	probably damaging	Het
Pxdc1	T	A	13: 34,652,195 (GRCm38)	T81S	probably benign	Het
Rnf150	A	T	8: 83,003,599 (GRCm38)	K253*	probably null	Het
Rsg1	C	T	4: 141,219,866 (GRCm38)	P186L	probably benign	Het
Sae1	T	C	7: 16,370,462 (GRCm38)		probably null	Het
Scin	C	T	12: 40,063,259 (GRCm38)	D538N	probably damaging	Het
Serpinb3c	C	A	1: 107,271,803 (GRCm38)	K329N	probably damaging	Het
Sgo2b	T	A	8: 63,926,974 (GRCm38)	K941N	possibly damaging	Het
Six1	C	T	12: 73,046,284 (GRCm38)	S48N	possibly damaging	Het
Slc39a8	G	A	3: 135,884,688 (GRCm38)	G381R	probably damaging	Het
Slc9b1	A	G	3: 135,357,559 (GRCm38)	K35E	unknown	Het
Srek1	C	T	13: 103,759,244 (GRCm38)	A274T	probably damaging	Het
Steap2	A	T	5: 5,677,497 (GRCm38)	Y279*	probably null	Het
Svil	T	A	18: 5,046,823 (GRCm38)	L110*	probably null	Het
Syncrip	T	C	9: 88,456,709 (GRCm38)		probably benign	Het
Tcf20	A	G	15: 82,853,242 (GRCm38)	I1336T	probably benign	Het
Tecpr1	T	A	5: 144,218,633 (GRCm38)	K36*	probably null	Het
Tenm2	A	G	11: 36,141,683 (GRCm38)	V670A	probably damaging	Het
Thbd	G	A	2: 148,407,366 (GRCm38)	T194I	probably damaging	Het
Tm9sf2	A	T	14: 122,151,962 (GRCm38)		probably null	Het
Tmtc4	A	T	14: 122,950,499 (GRCm38)	I225N	probably damaging	Het
Tpp1	C	T	7: 105,747,536 (GRCm38)	V425M	probably damaging	Het
Trbc2	T	A	6: 41,547,812 (GRCm38)	Y144*	probably null	Het
Trps1	T	C	15: 50,846,108 (GRCm38)	D282G	probably damaging	Het
Tsc22d4	T	A	5: 137,747,142 (GRCm38)	S9R	probably damaging	Het
Upp1	T	A	11: 9,136,025 (GRCm38)	D287E	probably benign	Het
Uso1	A	C	5: 92,201,299 (GRCm38)	Q916H	probably damaging	Het
Uty	T	A	Y: 1,134,902 (GRCm38)	Y884F	probably damaging	Het
Vmn1r222	T	C	13: 23,232,780 (GRCm38)	R88G	probably damaging	Het
Vmn1r79	T	C	7: 12,177,001 (GRCm38)	V270A	possibly damaging	Het
Zbtb22	T	C	17: 33,917,735 (GRCm38)	S285P	probably benign	Het
Zfp385a	A	G	15: 103,318,065 (GRCm38)	V82A	probably damaging	Het
Zfp59	T	C	7: 27,854,169 (GRCm38)	F349L	probably benign	Het
Zfp780b	T	G	7: 27,962,799 (GRCm38)	H777P	probably benign	Het
Zfp82	T	C	7: 30,057,124 (GRCm38)	T178A	probably benign	Het
Zfp957	A	G	14: 79,212,767 (GRCm38)	Y531H	probably damaging	Het

Other mutations in Zfp850

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02278:Zfp850	APN	7	28,008,397 (GRCm38)	missense	probably damaging	0.96
R0610:Zfp850	UTSW	7	27,989,394 (GRCm38)	missense	probably damaging	0.99
R0690:Zfp850	UTSW	7	27,985,217 (GRCm38)	missense	possibly damaging	0.67
R0711:Zfp850	UTSW	7	27,990,273 (GRCm38)	missense	probably benign	0.00
R1310:Zfp850	UTSW	7	27,989,459 (GRCm38)	missense	probably benign	0.40
R1594:Zfp850	UTSW	7	27,989,391 (GRCm38)	missense	probably benign	0.00
R1771:Zfp850	UTSW	7	27,985,275 (GRCm38)	nonsense	probably null
R2189:Zfp850	UTSW	7	27,989,055 (GRCm38)	missense	probably benign	0.02
R2192:Zfp850	UTSW	7	27,985,195 (GRCm38)	missense	probably damaging	1.00
R2417:Zfp850	UTSW	7	27,989,183 (GRCm38)	missense	possibly damaging	0.54
R4321:Zfp850	UTSW	7	27,989,400 (GRCm38)	missense	probably damaging	0.99
R4770:Zfp850	UTSW	7	27,984,986 (GRCm38)	splice site	probably null
R4970:Zfp850	UTSW	7	27,990,233 (GRCm38)	nonsense	probably null
R5112:Zfp850	UTSW	7	27,990,233 (GRCm38)	nonsense	probably null
R5166:Zfp850	UTSW	7	27,990,356 (GRCm38)	nonsense	probably null
R5303:Zfp850	UTSW	7	28,008,413 (GRCm38)	missense	probably damaging	1.00
R5315:Zfp850	UTSW	7	27,990,318 (GRCm38)	missense	probably benign	0.02
R5496:Zfp850	UTSW	7	28,007,346 (GRCm38)	missense	probably damaging	0.98
R5547:Zfp850	UTSW	7	27,989,419 (GRCm38)	missense	probably damaging	1.00
R5927:Zfp850	UTSW	7	27,990,195 (GRCm38)	missense	probably benign	0.17
R6654:Zfp850	UTSW	7	27,985,215 (GRCm38)	nonsense	probably null
R6950:Zfp850	UTSW	7	27,990,514 (GRCm38)	missense	possibly damaging	0.86
R6987:Zfp850	UTSW	7	27,990,001 (GRCm38)	missense	probably damaging	1.00
R6990:Zfp850	UTSW	7	27,990,376 (GRCm38)	missense	probably benign	0.09
R7640:Zfp850	UTSW	7	27,989,209 (GRCm38)	missense	probably benign	0.05
R7856:Zfp850	UTSW	7	27,990,474 (GRCm38)	missense	probably benign	0.02
R9498:Zfp850	UTSW	7	27,989,850 (GRCm38)	missense	possibly damaging	0.88
R9506:Zfp850	UTSW	7	27,989,562 (GRCm38)	missense	possibly damaging	0.82
Z1186:Zfp850	UTSW	7	27,990,279 (GRCm38)	missense	probably benign	0.00
Z1186:Zfp850	UTSW	7	27,989,124 (GRCm38)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ATCTCTGGTGGCGAATAAGATG -3'
(R):5'- AGCCTTCAGACTTCAGTCCC -3'

Sequencing Primer
(F):5'- CCAGTGTGAATACTCAGGTGCTC -3'
(R):5'- CAACATCAGAGAATTCATACTGGTG -3'

Posted On

2016-11-09