Mutagenetix > Incidental Mutation

Incidental Mutation 'R5677:Diaph1'

442860

Institutional Source

Beutler Lab

Gene Symbol

Diaph1

Ensembl Gene

ENSMUSG00000024456

Gene Name

diaphanous related formin 1

Synonyms

Drf1, Dia1, D18Wsu154e, mDia1, Diap1, p140mDia

MMRRC Submission

043316-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5677 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

37843601-37935476 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 37855951 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Lysine at position 910 (M910K)

Ref Sequence

ENSEMBL: ENSMUSP00000111297 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025337] [ENSMUST00000080033] [ENSMUST00000115629] [ENSMUST00000115631] [ENSMUST00000115634]

AlphaFold

O08808

PDB Structure

Crystal structure of the core FH2 domain of mouse mDia1 [X-RAY DIFFRACTION]
Crystal structure of mDIA1 GBD-FH3 in complex with RhoC-GMPPNP [X-RAY DIFFRACTION]
Crystal structure of the N-terminal mDia1 Armadillo Repeat Region and Dimerisation Domain in complex with the mDia1 autoregulatory domain (DAD) [X-RAY DIFFRACTION]
CRYSTAL STRUCTURE OF THE DIMERIC REGULATORY DOMAIN OF MOUSE DIAPHANEOUS-RELATED FORMIN (DRF), MDIA1 [X-RAY DIFFRACTION]
Crystal structure of the autoinhibitory switch in Formin mDia1; the DID/DAD complex [X-RAY DIFFRACTION]
Mouse Profilin IIa in complex with a double repeat from the FH1 domain of mDia1 [X-RAY DIFFRACTION]
Crystal structure of MDIA1-TSH GBD-FH3 in complex with CDC42-GMPPNP [X-RAY DIFFRACTION]
Crystal structure of complex between amino and carboxy terminal fragments of mDia1 [X-RAY DIFFRACTION]
Autoinhibited Formin mDia1 Structure [X-RAY DIFFRACTION]

Predicted Effect

probably damaging
Transcript: ENSMUST00000025337
AA Change: M919K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000025337
Gene: ENSMUSG00000024456
AA Change: M919K

Domain	Start	End	E-Value	Type
low complexity region	5	13	N/A	INTRINSIC
Drf_GBD	84	268	1.07e-57	SMART
Drf_FH3	274	466	2.06e-68	SMART
coiled coil region	471	571	N/A	INTRINSIC
Pfam:Drf_FH1	609	756	6.1e-43	PFAM
FH2	761	1206	2.46e-182	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000080033
AA Change: M910K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000078942
Gene: ENSMUSG00000024456
AA Change: M910K

Domain	Start	End	E-Value	Type
low complexity region	5	13	N/A	INTRINSIC
Drf_GBD	75	259	1.07e-57	SMART
Drf_FH3	265	457	2.06e-68	SMART
coiled coil region	462	562	N/A	INTRINSIC
Pfam:Drf_FH1	589	747	7.9e-52	PFAM
FH2	752	1197	3.73e-182	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000115629
AA Change: M875K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000111292
Gene: ENSMUSG00000024456
AA Change: M875K

Domain	Start	End	E-Value	Type
Drf_GBD	40	224	1.07e-57	SMART
Drf_FH3	230	422	2.06e-68	SMART
coiled coil region	427	527	N/A	INTRINSIC
Pfam:Drf_FH1	554	712	7.6e-52	PFAM
FH2	717	1162	3.73e-182	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000115631
AA Change: M875K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000111294
Gene: ENSMUSG00000024456
AA Change: M875K

Domain	Start	End	E-Value	Type
Drf_GBD	40	224	1.07e-57	SMART
Drf_FH3	230	422	2.06e-68	SMART
coiled coil region	427	527	N/A	INTRINSIC
Pfam:Drf_FH1	554	712	1.1e-51	PFAM
FH2	717	1162	2.46e-182	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000115634
AA Change: M910K

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000111297
Gene: ENSMUSG00000024456
AA Change: M910K

Domain	Start	End	E-Value	Type
low complexity region	5	13	N/A	INTRINSIC
Drf_GBD	75	259	1.07e-57	SMART
Drf_FH3	265	457	2.06e-68	SMART
coiled coil region	462	562	N/A	INTRINSIC
Pfam:Drf_FH1	589	747	9.4e-52	PFAM
FH2	752	1197	2.46e-182	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000124822

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127346

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000183927

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.5%
20x: 95.9%

Validation Efficiency

MGI Phenotype

FUNCTION: This gene encodes a member of the formin family of proteins that play important roles in cytoskeletal rearragnement by nucleation of actin filaments. Mice lacking the encoded protein develop age-dependent myeloproliferative defects resembling human myeloproliferative syndrome and myelodysplastic syndromes. Trafficking of T lymphocytes to secondary lymphoid organs and egression of thymocytes from the thymus are impaired in these animals. Lack of the encoded protein in T lymphocytes and thymocytes also reduces chemotaxis. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2016]
PHENOTYPE: Mice homozygous for a null allele exhibit abnormal hematopoiesis, bone marrow cell morphology, spleen morphology, skin physiology, skull morphology, and postnatal growth. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 116 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310057J18Rik	C	T	10: 28,986,229 (GRCm38)	V22I	probably benign	Het
4930430A15Rik	T	C	2: 111,211,565 (GRCm38)	T342A	probably benign	Het
4931423N10Rik	T	A	2: 23,212,718 (GRCm38)	L156Q	probably damaging	Het
Abca8a	A	T	11: 110,038,399 (GRCm38)	V1296D	possibly damaging	Het
Abca8b	C	T	11: 109,940,861 (GRCm38)	S1328N	probably damaging	Het
Adcy1	G	A	11: 7,161,914 (GRCm38)	M926I	probably damaging	Het
Aff4	G	T	11: 53,400,275 (GRCm38)	M687I	possibly damaging	Het
Agbl2	T	A	2: 90,807,978 (GRCm38)	Y636N	possibly damaging	Het
Agtr1a	A	T	13: 30,381,584 (GRCm38)	I211F	probably damaging	Het
Alkbh8	T	G	9: 3,385,147 (GRCm38)	S480A	possibly damaging	Het
Ankrd13b	A	T	11: 77,477,544 (GRCm38)	V84E	probably damaging	Het
Ap3b1	C	T	13: 94,528,196 (GRCm38)	T881I	unknown	Het
Apbb1	A	C	7: 105,559,246 (GRCm38)	D617E	probably damaging	Het
Apobec4	A	G	1: 152,757,282 (GRCm38)	R354G	probably benign	Het
BC003331	G	A	1: 150,374,837 (GRCm38)	L319F	probably damaging	Het
Brdt	T	G	5: 107,348,617 (GRCm38)	C198W	possibly damaging	Het
Cacna1b	G	T	2: 24,679,358 (GRCm38)	H851Q	possibly damaging	Het
Car2	G	T	3: 14,898,055 (GRCm38)	V217F	possibly damaging	Het
Ccdc24	A	C	4: 117,869,880 (GRCm38)		probably benign	Het
Chodl	C	A	16: 78,941,315 (GRCm38)	A57E	probably damaging	Het
Clgn	G	T	8: 83,409,538 (GRCm38)	C185F	probably damaging	Het
Cltc	G	T	11: 86,705,242 (GRCm38)	N1223K	probably damaging	Het
Cnot10	T	C	9: 114,629,093 (GRCm38)	N115S	probably damaging	Het
Col12a1	T	C	9: 79,699,321 (GRCm38)	R607G	probably damaging	Het
Cpd	A	G	11: 76,799,825 (GRCm38)	V835A	probably benign	Het
Csmd3	G	C	15: 48,622,051 (GRCm38)	L153V	probably damaging	Het
Ctr9	A	G	7: 111,044,002 (GRCm38)	H527R	probably benign	Het
Cwc22	G	A	2: 77,929,443 (GRCm38)	R87W	probably damaging	Het
D930020B18Rik	T	A	10: 121,669,201 (GRCm38)	N107K	probably benign	Het
Dgkg	C	A	16: 22,570,171 (GRCm38)	V418L	probably benign	Het
Dhx40	A	G	11: 86,800,963 (GRCm38)		probably null	Het
Diras2	T	A	13: 52,507,675 (GRCm38)	M199L	possibly damaging	Het
Dnah8	G	A	17: 30,748,568 (GRCm38)	D2585N	probably benign	Het
Dock5	T	A	14: 67,777,603 (GRCm38)	Q1302H	probably benign	Het
Dync1i2	T	C	2: 71,228,623 (GRCm38)	S90P	probably benign	Het
E2f8	A	T	7: 48,867,195 (GRCm38)	V812E	probably damaging	Het
Egfem1	C	A	3: 29,690,174 (GRCm38)	Q521K	probably damaging	Het
Fbxl18	A	T	5: 142,878,720 (GRCm38)	C699*	probably null	Het
Fgf3	C	T	7: 144,838,783 (GRCm38)	R26*	probably null	Het
Fpr-rs7	T	G	17: 20,114,103 (GRCm38)	I42L	probably benign	Het
Gm13101	A	T	4: 143,965,138 (GRCm38)	D338E	possibly damaging	Het
Gm3159	T	A	14: 4,398,582 (GRCm38)	M91K	probably damaging	Het
Gprin3	A	G	6: 59,353,892 (GRCm38)	S477P	possibly damaging	Het
Grm8	A	T	6: 27,761,204 (GRCm38)		probably null	Het
Hepacam2	A	T	6: 3,466,142 (GRCm38)	D420E	probably damaging	Het
Hmcn1	A	T	1: 150,609,778 (GRCm38)	W4358R	probably benign	Het
Ifna6	G	A	4: 88,827,719 (GRCm38)	A102T	probably benign	Het
Ighv2-2	T	C	12: 113,588,522 (GRCm38)	Q32R	probably benign	Het
Igkv1-131	T	A	6: 67,766,258 (GRCm38)	Q47L	possibly damaging	Het
Il16	T	C	7: 83,674,553 (GRCm38)	E263G	probably damaging	Het
Kansl1	A	T	11: 104,335,148 (GRCm38)	C981S	probably benign	Het
Lrp1	A	T	10: 127,574,429 (GRCm38)	F1483I	probably damaging	Het
Ltf	T	C	9: 111,020,912 (GRCm38)	M1T	probably null	Het
Ly75	C	T	2: 60,299,082 (GRCm38)	R1653H	probably benign	Het
Macrod2	T	C	2: 142,176,667 (GRCm38)	F240S	probably damaging	Het
Man1c1	T	C	4: 134,569,060 (GRCm38)	E433G	probably damaging	Het
Mansc4	A	T	6: 147,081,549 (GRCm38)	M130K	probably benign	Het
Mccc1	C	T	3: 35,990,048 (GRCm38)		probably null	Het
Mink1	G	T	11: 70,605,165 (GRCm38)	R75L	possibly damaging	Het
Mst1	A	G	9: 108,081,286 (GRCm38)	D65G	probably damaging	Het
Myo9b	C	T	8: 71,343,686 (GRCm38)	A857V	probably damaging	Het
Ndufa4	A	G	6: 11,900,575 (GRCm38)	V70A	probably benign	Het
Npat	T	A	9: 53,555,100 (GRCm38)	S230T	probably benign	Het
Nr1d1	A	G	11: 98,771,308 (GRCm38)	Y167H	probably damaging	Het
Oca2	A	G	7: 56,414,462 (GRCm38)	D735G	probably damaging	Het
Olfr13	T	C	6: 43,174,331 (GRCm38)	V115A	probably benign	Het
Olfr1359	C	T	13: 21,703,223 (GRCm38)	T74I	probably benign	Het
Olfr1383	A	G	11: 49,523,944 (GRCm38)	T74A	probably damaging	Het
Olfr835	T	A	9: 19,035,558 (GRCm38)	I145N	possibly damaging	Het
Otop1	A	G	5: 38,300,163 (GRCm38)	Y422C	probably damaging	Het
Pde4dip	G	A	3: 97,841,648 (GRCm38)	R126*	probably null	Het
Pdp2	C	T	8: 104,594,688 (GRCm38)	P390S	probably damaging	Het
Pds5b	A	T	5: 150,716,461 (GRCm38)	T14S	possibly damaging	Het
Pfkp	T	C	13: 6,588,595 (GRCm38)	E580G	probably damaging	Het
Piezo2	G	C	18: 63,117,697 (GRCm38)	L444V	probably benign	Het
Piezo2	A	T	18: 63,117,696 (GRCm38)	L212Q	possibly damaging	Het
Pla2g4d	T	A	2: 120,278,948 (GRCm38)	T207S	possibly damaging	Het
Plk2	C	A	13: 110,399,057 (GRCm38)	T471K	possibly damaging	Het
Ppp1r3g	G	A	13: 35,969,262 (GRCm38)	E222K	probably damaging	Het
Prkcg	A	G	7: 3,323,458 (GRCm38)	D480G	probably damaging	Het
Pxdc1	T	A	13: 34,652,195 (GRCm38)	T81S	probably benign	Het
Rnf150	A	T	8: 83,003,599 (GRCm38)	K253*	probably null	Het
Rsg1	C	T	4: 141,219,866 (GRCm38)	P186L	probably benign	Het
Sae1	T	C	7: 16,370,462 (GRCm38)		probably null	Het
Scin	C	T	12: 40,063,259 (GRCm38)	D538N	probably damaging	Het
Serpinb3c	C	A	1: 107,271,803 (GRCm38)	K329N	probably damaging	Het
Sgo2b	T	A	8: 63,926,974 (GRCm38)	K941N	possibly damaging	Het
Six1	C	T	12: 73,046,284 (GRCm38)	S48N	possibly damaging	Het
Slc39a8	G	A	3: 135,884,688 (GRCm38)	G381R	probably damaging	Het
Slc9b1	A	G	3: 135,357,559 (GRCm38)	K35E	unknown	Het
Srek1	C	T	13: 103,759,244 (GRCm38)	A274T	probably damaging	Het
Steap2	A	T	5: 5,677,497 (GRCm38)	Y279*	probably null	Het
Svil	T	A	18: 5,046,823 (GRCm38)	L110*	probably null	Het
Syncrip	T	C	9: 88,456,709 (GRCm38)		probably benign	Het
Tcf20	A	G	15: 82,853,242 (GRCm38)	I1336T	probably benign	Het
Tecpr1	T	A	5: 144,218,633 (GRCm38)	K36*	probably null	Het
Tenm2	A	G	11: 36,141,683 (GRCm38)	V670A	probably damaging	Het
Thbd	G	A	2: 148,407,366 (GRCm38)	T194I	probably damaging	Het
Tm9sf2	A	T	14: 122,151,962 (GRCm38)		probably null	Het
Tmtc4	A	T	14: 122,950,499 (GRCm38)	I225N	probably damaging	Het
Tpp1	C	T	7: 105,747,536 (GRCm38)	V425M	probably damaging	Het
Trbc2	T	A	6: 41,547,812 (GRCm38)	Y144*	probably null	Het
Trps1	T	C	15: 50,846,108 (GRCm38)	D282G	probably damaging	Het
Tsc22d4	T	A	5: 137,747,142 (GRCm38)	S9R	probably damaging	Het
Upp1	T	A	11: 9,136,025 (GRCm38)	D287E	probably benign	Het
Uso1	A	C	5: 92,201,299 (GRCm38)	Q916H	probably damaging	Het
Uty	T	A	Y: 1,134,902 (GRCm38)	Y884F	probably damaging	Het
Vmn1r222	T	C	13: 23,232,780 (GRCm38)	R88G	probably damaging	Het
Vmn1r79	T	C	7: 12,177,001 (GRCm38)	V270A	possibly damaging	Het
Zbtb22	T	C	17: 33,917,735 (GRCm38)	S285P	probably benign	Het
Zfp385a	A	G	15: 103,318,065 (GRCm38)	V82A	probably damaging	Het
Zfp59	T	C	7: 27,854,169 (GRCm38)	F349L	probably benign	Het
Zfp780b	T	G	7: 27,962,799 (GRCm38)	H777P	probably benign	Het
Zfp82	T	C	7: 30,057,124 (GRCm38)	T178A	probably benign	Het
Zfp850	T	C	7: 27,989,088 (GRCm38)	Y565C	probably damaging	Het
Zfp957	A	G	14: 79,212,767 (GRCm38)	Y531H	probably damaging	Het

Other mutations in Diaph1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00518:Diaph1	APN	18	37,893,348 (GRCm38)	critical splice donor site	probably null
IGL01432:Diaph1	APN	18	37,897,504 (GRCm38)	missense	unknown
IGL01646:Diaph1	APN	18	37,893,416 (GRCm38)	critical splice acceptor site	probably null
IGL01676:Diaph1	APN	18	37,856,188 (GRCm38)	nonsense	probably null
IGL01731:Diaph1	APN	18	37,853,709 (GRCm38)	critical splice acceptor site	probably benign
IGL01921:Diaph1	APN	18	37,856,208 (GRCm38)	missense	possibly damaging	0.73
IGL02200:Diaph1	APN	18	37,890,682 (GRCm38)	missense	unknown
IGL02258:Diaph1	APN	18	37,853,330 (GRCm38)	missense	probably damaging	0.99
IGL02325:Diaph1	APN	18	37,853,600 (GRCm38)	missense	probably damaging	1.00
IGL03304:Diaph1	APN	18	37,854,573 (GRCm38)	missense	possibly damaging	0.47
albatross	UTSW	18	37,853,679 (GRCm38)	nonsense	probably null
cucamonga	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
damselfly	UTSW	18	37,897,550 (GRCm38)	nonsense	probably null
devastator	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
fishnets	UTSW	18	37,895,300 (GRCm38)	critical splice acceptor site	probably null
Guangzhou	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
saran	UTSW	18	37,855,804 (GRCm38)	missense	probably damaging	1.00
seethrough	UTSW	18	37,889,769 (GRCm38)	missense	probably damaging	1.00
sheer	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably benign
R0137:Diaph1	UTSW	18	37,891,849 (GRCm38)	missense	unknown
R0446:Diaph1	UTSW	18	37,853,590 (GRCm38)	missense	possibly damaging	0.94
R0523:Diaph1	UTSW	18	37,856,500 (GRCm38)	missense	possibly damaging	0.56
R1433:Diaph1	UTSW	18	37,905,134 (GRCm38)	missense	unknown
R1532:Diaph1	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
R1534:Diaph1	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
R1535:Diaph1	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
R1536:Diaph1	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
R1537:Diaph1	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably null
R1611:Diaph1	UTSW	18	37,900,702 (GRCm38)	missense	unknown
R1756:Diaph1	UTSW	18	37,854,573 (GRCm38)	missense	possibly damaging	0.47
R1771:Diaph1	UTSW	18	37,891,018 (GRCm38)	missense	unknown
R1812:Diaph1	UTSW	18	37,891,018 (GRCm38)	missense	unknown
R2121:Diaph1	UTSW	18	37,896,389 (GRCm38)	missense	unknown
R3710:Diaph1	UTSW	18	37,845,484 (GRCm38)	missense	probably damaging	1.00
R3891:Diaph1	UTSW	18	37,900,638 (GRCm38)	splice site	probably benign
R3892:Diaph1	UTSW	18	37,900,638 (GRCm38)	splice site	probably benign
R4077:Diaph1	UTSW	18	37,853,583 (GRCm38)	missense	possibly damaging	0.68
R4079:Diaph1	UTSW	18	37,853,583 (GRCm38)	missense	possibly damaging	0.68
R4771:Diaph1	UTSW	18	37,853,551 (GRCm38)	missense	probably damaging	1.00
R4815:Diaph1	UTSW	18	37,895,203 (GRCm38)	missense	unknown
R5242:Diaph1	UTSW	18	37,851,635 (GRCm38)	missense	probably damaging	1.00
R5294:Diaph1	UTSW	18	37,897,580 (GRCm38)	missense	unknown
R5294:Diaph1	UTSW	18	37,897,550 (GRCm38)	nonsense	probably null
R5349:Diaph1	UTSW	18	37,891,072 (GRCm38)	missense	unknown
R5427:Diaph1	UTSW	18	37,890,595 (GRCm38)	missense	unknown
R5623:Diaph1	UTSW	18	37,896,093 (GRCm38)	critical splice donor site	probably benign
R5730:Diaph1	UTSW	18	37,903,776 (GRCm38)	missense	unknown
R5767:Diaph1	UTSW	18	37,853,355 (GRCm38)	missense	probably damaging	1.00
R5925:Diaph1	UTSW	18	37,891,935 (GRCm38)	missense	unknown
R6151:Diaph1	UTSW	18	37,853,353 (GRCm38)	missense	probably damaging	1.00
R6823:Diaph1	UTSW	18	37,876,383 (GRCm38)	splice site	probably null
R6876:Diaph1	UTSW	18	37,896,373 (GRCm38)	missense	unknown
R6925:Diaph1	UTSW	18	37,853,679 (GRCm38)	nonsense	probably null
R6983:Diaph1	UTSW	18	37,889,769 (GRCm38)	missense	probably damaging	1.00
R7073:Diaph1	UTSW	18	37,889,814 (GRCm38)	critical splice acceptor site	probably null
R7248:Diaph1	UTSW	18	37,889,776 (GRCm38)	missense	probably benign	0.26
R7400:Diaph1	UTSW	18	37,854,502 (GRCm38)	missense	probably damaging	1.00
R7497:Diaph1	UTSW	18	37,895,300 (GRCm38)	critical splice acceptor site	probably null
R7544:Diaph1	UTSW	18	37,893,269 (GRCm38)	splice site	probably null
R7703:Diaph1	UTSW	18	37,890,809 (GRCm38)	missense	unknown
R7834:Diaph1	UTSW	18	37,853,709 (GRCm38)	critical splice acceptor site	probably benign
R8073:Diaph1	UTSW	18	37,891,797 (GRCm38)	missense	unknown
R8378:Diaph1	UTSW	18	37,891,953 (GRCm38)	missense	unknown
R8847:Diaph1	UTSW	18	37,854,537 (GRCm38)	missense	possibly damaging	0.71
R8947:Diaph1	UTSW	18	37,853,701 (GRCm38)	missense	probably damaging	1.00
R8990:Diaph1	UTSW	18	37,855,804 (GRCm38)	missense	probably damaging	1.00
R9059:Diaph1	UTSW	18	37,889,745 (GRCm38)	missense	possibly damaging	0.53
R9189:Diaph1	UTSW	18	37,891,109 (GRCm38)	missense	unknown
R9297:Diaph1	UTSW	18	37,889,775 (GRCm38)	missense	probably benign	0.26
R9438:Diaph1	UTSW	18	37,893,390 (GRCm38)	missense	unknown
R9439:Diaph1	UTSW	18	37,896,359 (GRCm38)	critical splice donor site	probably null
R9538:Diaph1	UTSW	18	37,853,417 (GRCm38)	missense	probably damaging	1.00
R9596:Diaph1	UTSW	18	37,891,058 (GRCm38)	missense	unknown
R9752:Diaph1	UTSW	18	37,903,071 (GRCm38)	missense	unknown
R9762:Diaph1	UTSW	18	37,854,536 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TTTCCGACCAGCAGTGTGAG -3'
(R):5'- CTGGCTGAGTCAGAGCAGTTTG -3'

Sequencing Primer
(F):5'- ACCAGCAGTGTGAGCTCCAG -3'
(R):5'- TAAGGCCTGGAGCAGTCACTC -3'

Posted On

2016-11-09