Mutagenetix > Incidental Mutation

Incidental Mutation 'R5683:Vmn2r104'

443204

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r104

Ensembl Gene

ENSMUSG00000090315

Gene Name

vomeronasal 2, receptor 104

Synonyms

V2r7

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.124) question?

Stock #

R5683 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20029425-20048205 bp(-) (GRCm38)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 20040719 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Lysine to Stop codon at position 481 (K481*)

Ref Sequence

ENSEMBL: ENSMUSP00000129895 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000168050]

AlphaFold

E9Q2J5

Predicted Effect

probably null
Transcript: ENSMUST00000168050
AA Change: K481*

SMART Domains

Protein: ENSMUSP00000129895
Gene: ENSMUSG00000090315
AA Change: K481*

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	85	457	4e-38	PFAM
Pfam:NCD3G	512	565	2.1e-20	PFAM
Pfam:7tm_3	598	833	1.7e-52	PFAM

Coding Region Coverage

1x: 99.6%
3x: 98.8%
10x: 97.0%
20x: 94.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 45 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933427D14Rik	A	C	11: 72,202,440 (GRCm38)	M22R	probably benign	Het
Acad11	A	G	9: 104,084,283 (GRCm38)	E397G	probably damaging	Het
Actrt3	A	T	3: 30,598,278 (GRCm38)	D222E	probably benign	Het
Akap11	A	T	14: 78,512,578 (GRCm38)	S790T	probably damaging	Het
Arhgap5	T	A	12: 52,519,586 (GRCm38)	D1113E	probably benign	Het
Arl2	C	A	19: 6,134,764 (GRCm38)	R153L	probably benign	Het
B3glct	T	A	5: 149,696,437 (GRCm38)	M19K	probably benign	Het
Ccdc138	C	A	10: 58,540,819 (GRCm38)	Q425K	probably damaging	Het
Ccdc178	T	A	18: 22,130,122 (GRCm38)	K143N	probably benign	Het
Cd200r4	T	A	16: 44,832,948 (GRCm38)	I73K	probably benign	Het
Chaf1b	A	G	16: 93,887,142 (GRCm38)	K94E	possibly damaging	Het
Cmtm2a	T	C	8: 104,293,044 (GRCm38)		probably null	Het
Dnhd1	G	A	7: 105,703,209 (GRCm38)	R2523Q	probably damaging	Het
Fnbp4	C	A	2: 90,752,862 (GRCm38)	N277K	probably damaging	Het
Htr7	C	A	19: 35,969,871 (GRCm38)	A248S	probably damaging	Het
Itsn1	T	C	16: 91,905,380 (GRCm38)	Y37H	probably benign	Het
Kdm8	A	G	7: 125,455,173 (GRCm38)	Y16C	possibly damaging	Het
Kif1b	A	T	4: 149,222,261 (GRCm38)	Y881N	probably damaging	Het
Lhcgr	C	T	17: 88,772,019 (GRCm38)	V80I	probably benign	Het
Lrriq1	T	A	10: 103,173,375 (GRCm38)	L1082F	probably damaging	Het
Met	A	G	6: 17,571,744 (GRCm38)	Y1354C	probably damaging	Het
Nbea	A	G	3: 55,628,586 (GRCm38)	L2859P	possibly damaging	Het
Ndufaf5	T	A	2: 140,202,923 (GRCm38)	M279K	possibly damaging	Het
Nlrp4e	T	C	7: 23,353,272 (GRCm38)	I872T	probably damaging	Het
Npnt	A	T	3: 132,906,840 (GRCm38)		probably null	Het
Nsd1	G	A	13: 55,246,148 (GRCm38)	V521I	probably benign	Het
Olfr138	G	A	17: 38,275,546 (GRCm38)	M258I	possibly damaging	Het
Pak6	T	A	2: 118,693,912 (GRCm38)	Y469N	probably damaging	Het
Parp4	C	T	14: 56,647,429 (GRCm38)	R1322*	probably null	Het
Pax6	T	C	2: 105,685,907 (GRCm38)	Y177H	probably benign	Het
Pcdhb4	A	T	18: 37,308,989 (GRCm38)	T451S	probably benign	Het
Pcdhgb5	A	T	18: 37,731,854 (GRCm38)	D234V	probably damaging	Het
Pramef6	A	G	4: 143,895,853 (GRCm38)	S311P	probably damaging	Het
Ralb	G	A	1: 119,475,956 (GRCm38)	A147V	possibly damaging	Het
Rgs11	A	G	17: 26,205,181 (GRCm38)	K196E	probably benign	Het
Rnft1	A	T	11: 86,491,790 (GRCm38)	T280S	probably benign	Het
Slco4c1	G	T	1: 96,867,834 (GRCm38)	H166Q	probably damaging	Het
Sycp3	T	C	10: 88,472,935 (GRCm38)	S248P	probably damaging	Het
Tab2	A	G	10: 7,919,112 (GRCm38)		probably null	Het
Tgm6	T	C	2: 130,138,955 (GRCm38)	M224T	probably damaging	Het
Topbp1	A	T	9: 103,312,804 (GRCm38)	E193V	possibly damaging	Het
Trim36	T	C	18: 46,169,292 (GRCm38)	Y551C	probably damaging	Het
Ttc17	T	C	2: 94,362,521 (GRCm38)	Y628C	probably damaging	Het
Vmn1r180	A	C	7: 23,953,210 (GRCm38)	D266A	possibly damaging	Het
Zc3hav1	A	G	6: 38,307,237 (GRCm38)	V981A	probably damaging	Het

Other mutations in Vmn2r104

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00427:Vmn2r104	APN	17	20,038,239 (GRCm38)	missense	probably damaging	0.98
IGL01098:Vmn2r104	APN	17	20,048,096 (GRCm38)	missense	probably benign	0.27
IGL01333:Vmn2r104	APN	17	20,042,793 (GRCm38)	missense	probably benign	0.17
IGL01527:Vmn2r104	APN	17	20,042,896 (GRCm38)	missense	possibly damaging	0.82
IGL01773:Vmn2r104	APN	17	20,040,668 (GRCm38)	missense	probably benign	0.10
IGL01939:Vmn2r104	APN	17	20,029,925 (GRCm38)	missense	probably damaging	0.99
IGL02121:Vmn2r104	APN	17	20,041,794 (GRCm38)	nonsense	probably null
IGL02305:Vmn2r104	APN	17	20,042,856 (GRCm38)	missense	probably benign	0.09
IGL02374:Vmn2r104	APN	17	20,042,786 (GRCm38)	missense	probably benign	0.34
IGL03260:Vmn2r104	APN	17	20,042,821 (GRCm38)	missense	probably benign	0.05
IGL03366:Vmn2r104	APN	17	20,029,604 (GRCm38)	missense	probably damaging	1.00
R0091:Vmn2r104	UTSW	17	20,041,813 (GRCm38)	missense	possibly damaging	0.79
R0125:Vmn2r104	UTSW	17	20,029,807 (GRCm38)	missense	probably damaging	0.98
R0257:Vmn2r104	UTSW	17	20,029,627 (GRCm38)	missense	probably damaging	1.00
R0381:Vmn2r104	UTSW	17	20,048,002 (GRCm38)	nonsense	probably null
R0709:Vmn2r104	UTSW	17	20,042,904 (GRCm38)	missense	probably damaging	1.00
R0786:Vmn2r104	UTSW	17	20,042,725 (GRCm38)	missense	probably benign
R1575:Vmn2r104	UTSW	17	20,042,215 (GRCm38)	missense	probably damaging	1.00
R1827:Vmn2r104	UTSW	17	20,042,235 (GRCm38)	missense	probably damaging	0.97
R1932:Vmn2r104	UTSW	17	20,040,769 (GRCm38)	missense	probably damaging	1.00
R1956:Vmn2r104	UTSW	17	20,042,051 (GRCm38)	missense	probably damaging	0.98
R2203:Vmn2r104	UTSW	17	20,029,821 (GRCm38)	missense	probably benign	0.05
R2205:Vmn2r104	UTSW	17	20,029,821 (GRCm38)	missense	probably benign	0.05
R2859:Vmn2r104	UTSW	17	20,048,193 (GRCm38)	missense	possibly damaging	0.82
R3701:Vmn2r104	UTSW	17	20,029,556 (GRCm38)	missense	probably damaging	1.00
R3834:Vmn2r104	UTSW	17	20,029,921 (GRCm38)	missense	probably benign	0.02
R4151:Vmn2r104	UTSW	17	20,029,885 (GRCm38)	missense	probably damaging	1.00
R4470:Vmn2r104	UTSW	17	20,042,241 (GRCm38)	missense	probably damaging	1.00
R4625:Vmn2r104	UTSW	17	20,048,181 (GRCm38)	missense	probably benign	0.00
R4754:Vmn2r104	UTSW	17	20,040,768 (GRCm38)	nonsense	probably null
R4911:Vmn2r104	UTSW	17	20,030,026 (GRCm38)	missense	probably benign	0.00
R5270:Vmn2r104	UTSW	17	20,038,266 (GRCm38)	missense	probably damaging	1.00
R5279:Vmn2r104	UTSW	17	20,041,884 (GRCm38)	missense	probably benign	0.07
R5311:Vmn2r104	UTSW	17	20,029,901 (GRCm38)	missense	probably damaging	1.00
R5370:Vmn2r104	UTSW	17	20,030,188 (GRCm38)	missense	probably damaging	0.97
R5461:Vmn2r104	UTSW	17	20,030,081 (GRCm38)	missense	probably damaging	1.00
R5795:Vmn2r104	UTSW	17	20,030,110 (GRCm38)	missense	probably benign	0.02
R5795:Vmn2r104	UTSW	17	20,030,282 (GRCm38)	missense	possibly damaging	0.89
R5970:Vmn2r104	UTSW	17	20,029,471 (GRCm38)	missense	probably benign	0.01
R5983:Vmn2r104	UTSW	17	20,041,708 (GRCm38)	missense	probably damaging	1.00
R5992:Vmn2r104	UTSW	17	20,029,485 (GRCm38)	missense	probably damaging	1.00
R6066:Vmn2r104	UTSW	17	20,038,311 (GRCm38)	missense	possibly damaging	0.69
R6156:Vmn2r104	UTSW	17	20,041,647 (GRCm38)	missense	probably damaging	1.00
R6182:Vmn2r104	UTSW	17	20,030,245 (GRCm38)	missense	probably benign	0.16
R6245:Vmn2r104	UTSW	17	20,041,567 (GRCm38)	missense	possibly damaging	0.69
R6333:Vmn2r104	UTSW	17	20,029,586 (GRCm38)	missense	probably benign	0.30
R6573:Vmn2r104	UTSW	17	20,042,225 (GRCm38)	missense	probably damaging	1.00
R7101:Vmn2r104	UTSW	17	20,030,096 (GRCm38)	missense	possibly damaging	0.65
R7123:Vmn2r104	UTSW	17	20,040,826 (GRCm38)	missense	probably benign	0.12
R7485:Vmn2r104	UTSW	17	20,029,475 (GRCm38)	missense	probably benign	0.01
R7514:Vmn2r104	UTSW	17	20,029,529 (GRCm38)	missense	probably damaging	1.00
R7634:Vmn2r104	UTSW	17	20,041,709 (GRCm38)	missense	possibly damaging	0.48
R7958:Vmn2r104	UTSW	17	20,042,726 (GRCm38)	missense	probably benign
R8031:Vmn2r104	UTSW	17	20,042,786 (GRCm38)	missense	probably benign	0.34
R8094:Vmn2r104	UTSW	17	20,030,221 (GRCm38)	missense	possibly damaging	0.77
R8191:Vmn2r104	UTSW	17	20,030,203 (GRCm38)	missense	possibly damaging	0.89
R8308:Vmn2r104	UTSW	17	20,040,778 (GRCm38)	missense	possibly damaging	0.55
R8691:Vmn2r104	UTSW	17	20,041,848 (GRCm38)	missense	probably damaging	0.98
R8795:Vmn2r104	UTSW	17	20,042,726 (GRCm38)	missense	probably benign
R8900:Vmn2r104	UTSW	17	20,041,662 (GRCm38)	missense	probably damaging	0.99
R8913:Vmn2r104	UTSW	17	20,029,706 (GRCm38)	missense	probably damaging	1.00
R9180:Vmn2r104	UTSW	17	20,042,825 (GRCm38)	missense	probably benign	0.00
R9199:Vmn2r104	UTSW	17	20,041,835 (GRCm38)	missense	probably damaging	0.99
R9282:Vmn2r104	UTSW	17	20,040,836 (GRCm38)	missense	probably damaging	1.00
R9303:Vmn2r104	UTSW	17	20,048,177 (GRCm38)	missense	possibly damaging	0.90
R9305:Vmn2r104	UTSW	17	20,048,177 (GRCm38)	missense	possibly damaging	0.90
R9322:Vmn2r104	UTSW	17	20,042,825 (GRCm38)	missense	probably benign	0.00
R9325:Vmn2r104	UTSW	17	20,048,171 (GRCm38)	missense	possibly damaging	0.95
R9414:Vmn2r104	UTSW	17	20,029,988 (GRCm38)	missense	probably damaging	0.99
R9785:Vmn2r104	UTSW	17	20,048,147 (GRCm38)	missense	probably benign
RF007:Vmn2r104	UTSW	17	20,048,040 (GRCm38)	missense	probably benign	0.36
Z1177:Vmn2r104	UTSW	17	20,029,789 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CGAGTATACTAATGTTGCCTTATGG -3'
(R):5'- TCTGCACATTGGGCACAAG -3'

Sequencing Primer
(F):5'- GTTGCCTTATGGAATTTTATACTG -3'
(R):5'- CCTGAAGGACATTGAAGTGA -3'

Posted On

2016-11-09