Mutagenetix > Incidental Mutation

Incidental Mutation 'R5686:Vmn2r90'

443391

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r90

Ensembl Gene

ENSMUSG00000092120

Gene Name

vomeronasal 2, receptor 90

Synonyms

EG626942

MMRRC Submission

043319-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.116) question?

Stock #

R5686 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

17703747-17735156 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 17713450 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Phenylalanine at position 424 (Y424F)

Ref Sequence

ENSEMBL: ENSMUSP00000126650 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000169805] [ENSMUST00000231659] [ENSMUST00000232078] [ENSMUST00000232113]

AlphaFold

E9PXJ8

Predicted Effect

probably benign
Transcript: ENSMUST00000169805
AA Change: Y424F

PolyPhen 2 Score 0.019 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000126650
Gene: ENSMUSG00000092120
AA Change: Y424F

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.8e-43	PFAM
Pfam:NCD3G	514	567	3e-22	PFAM
Pfam:7tm_3	600	835	9.8e-54	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000231659

Predicted Effect

probably benign
Transcript: ENSMUST00000232078

Predicted Effect

probably benign
Transcript: ENSMUST00000232113

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.7%
10x: 97.2%
20x: 95.1%

Validation Efficiency

97% (73/75)

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2210408I21Rik	A	G	13: 77,303,314	E839G	possibly damaging	Het
2410141K09Rik	T	C	13: 66,431,663	R254G	probably benign	Het
4932438A13Rik	T	A	3: 36,917,660	F514Y	probably benign	Het
Adgrf3	T	A	5: 30,197,306	T575S	probably damaging	Het
Akap9	T	A	5: 3,971,926	C1158S	probably benign	Het
Arhgap39	A	G	15: 76,726,633	F926L	probably damaging	Het
BC035947	G	T	1: 78,497,930	T655K	probably benign	Het
Bcas1	T	C	2: 170,406,810	T64A	probably benign	Het
Brca2	A	G	5: 150,540,904	K1378E	probably benign	Het
Card6	A	T	15: 5,100,953	N320K	probably damaging	Het
Ccdc3	A	T	2: 5,138,060	I43F	probably damaging	Het
Cd200r1	T	C	16: 44,790,164	S212P	probably damaging	Het
Cdh8	T	C	8: 99,033,222	I632V	probably benign	Het
Col25a1	A	G	3: 130,564,154	E477G	probably damaging	Het
Cpne5	A	T	17: 29,184,017	C215S	possibly damaging	Het
Crim1	T	A	17: 78,374,083	S989T	possibly damaging	Het
Dnhd1	G	A	7: 105,703,209	R2523Q	probably damaging	Het
Dync1h1	T	A	12: 110,616,404	N340K	probably benign	Het
Eif4e2	G	A	1: 87,226,238		probably null	Het
Ephb6	G	T	6: 41,619,704	R895L	possibly damaging	Het
Esrrg	T	A	1: 188,150,198	H217Q	probably benign	Het
Fgl1	T	A	8: 41,200,557	K100*	probably null	Het
Flt4	T	C	11: 49,630,603	V450A	probably benign	Het
G6pc2	A	T	2: 69,220,784	I74L	probably benign	Het
Gabrr1	T	C	4: 33,161,684	M336T	probably damaging	Het
Gli1	T	A	10: 127,337,436	T118S	probably benign	Het
Gm5435	A	T	12: 82,496,026		noncoding transcript	Het
Got1l1	A	G	8: 27,198,059	L314P	probably damaging	Het
Hk3	T	A	13: 55,006,813	I740F	probably damaging	Het
Htr7	C	A	19: 35,969,871	A248S	probably damaging	Het
Igsf9b	A	G	9: 27,324,179	T508A	probably damaging	Het
Il16	T	A	7: 83,648,728	N431I	probably benign	Het
Lax1	G	A	1: 133,680,176	P276S	probably damaging	Het
Lrp2	A	T	2: 69,511,061	V925E	possibly damaging	Het
Lrp3	T	A	7: 35,203,485	T479S	possibly damaging	Het
Metrn	G	A	17: 25,795,217	R212C	probably damaging	Het
Mlip	A	C	9: 77,347,693		probably null	Het
Mmp24	C	T	2: 155,799,777	T175I	probably damaging	Het
N6amt1	A	T	16: 87,354,335	D28V	probably damaging	Het
Olfr1289	G	A	2: 111,484,143	G238R	probably damaging	Het
Olfr215	T	C	6: 116,582,929	T6A	probably benign	Het
Olfr380	A	T	11: 73,453,851	Y120*	probably null	Het
Olfr472	A	G	7: 107,902,912	H65R	probably damaging	Het
Olfr490	G	T	7: 108,286,742	A128E	probably damaging	Het
Olfr870	T	A	9: 20,170,969	I201F	possibly damaging	Het
Pcdh17	T	A	14: 84,532,993	N970K	probably damaging	Het
Pdzrn3	T	C	6: 101,151,428	Y759C	probably damaging	Het
Pkd2l2	T	C	18: 34,425,237	L323P	probably damaging	Het
Psg21	G	T	7: 18,652,258		probably benign	Het
Rest	T	C	5: 77,281,726	V664A	probably benign	Het
Sco2	G	A	15: 89,371,972	R160*	probably null	Het
Sfswap	T	A	5: 129,514,818	S300T	probably damaging	Het
Slc5a10	A	T	11: 61,678,566	M329K	probably benign	Het
Slco1a5	G	A	6: 142,236,307	P564S	probably damaging	Het
Stk38	A	G	17: 28,982,129	F191S	probably damaging	Het
Svep1	T	A	4: 58,072,826	Y2161F	possibly damaging	Het
Tada2a	G	A	11: 84,079,602	T441M	possibly damaging	Het
Tg	T	A	15: 66,688,889	N1033K	probably benign	Het
Thoc3	A	T	13: 54,467,873	I126N	probably damaging	Het
Tnc	T	C	4: 64,007,730		probably null	Het
Tnc	A	T	4: 64,008,795	D831E	possibly damaging	Het
Uhmk1	A	T	1: 170,211,218	V100E	probably damaging	Het
Usp43	G	A	11: 67,921,916		probably benign	Het
Vps33a	C	T	5: 123,547,001		probably null	Het
Xirp2	A	T	2: 67,482,298	K37I	probably damaging	Het
Zfp106	A	T	2: 120,533,507		probably null	Het
Zfp748	A	T	13: 67,542,528	C204*	probably null	Het

Other mutations in Vmn2r90

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00092:Vmn2r90	APN	17	17733496	missense	probably benign	0.00
IGL01330:Vmn2r90	APN	17	17733280	missense	probably benign	0.00
IGL01989:Vmn2r90	APN	17	17713232	nonsense	probably null
IGL02080:Vmn2r90	APN	17	17712858	missense	probably damaging	1.00
IGL02112:Vmn2r90	APN	17	17712203	missense	probably damaging	0.98
IGL02123:Vmn2r90	APN	17	17733482	missense	probably benign	0.01
IGL02749:Vmn2r90	APN	17	17726860	makesense	probably null
IGL03114:Vmn2r90	APN	17	17733509	missense	probably damaging	1.00
R0131:Vmn2r90	UTSW	17	17712249	missense	probably benign	0.00
R0131:Vmn2r90	UTSW	17	17712249	missense	probably benign	0.00
R0132:Vmn2r90	UTSW	17	17712249	missense	probably benign	0.00
R0184:Vmn2r90	UTSW	17	17726877	nonsense	probably null
R0379:Vmn2r90	UTSW	17	17728139	missense	probably damaging	1.00
R1022:Vmn2r90	UTSW	17	17728138	missense	probably damaging	1.00
R1024:Vmn2r90	UTSW	17	17728138	missense	probably damaging	1.00
R1897:Vmn2r90	UTSW	17	17733304	missense	probably damaging	1.00
R1993:Vmn2r90	UTSW	17	17713263	missense	probably damaging	1.00
R2472:Vmn2r90	UTSW	17	17728146	missense	probably damaging	1.00
R2508:Vmn2r90	UTSW	17	17733967	missense	probably damaging	1.00
R2679:Vmn2r90	UTSW	17	17712869	missense	possibly damaging	0.46
R3409:Vmn2r90	UTSW	17	17733376	missense	probably benign	0.00
R4693:Vmn2r90	UTSW	17	17733694	missense	possibly damaging	0.91
R4797:Vmn2r90	UTSW	17	17712305	missense	probably damaging	1.00
R5258:Vmn2r90	UTSW	17	17712852	missense	probably benign	0.12
R5358:Vmn2r90	UTSW	17	17704150	critical splice donor site	probably null
R5445:Vmn2r90	UTSW	17	17734124	missense	probably benign	0.04
R5446:Vmn2r90	UTSW	17	17712202	missense	probably damaging	0.99
R5567:Vmn2r90	UTSW	17	17712074	missense	probably damaging	1.00
R5680:Vmn2r90	UTSW	17	17726772	missense	possibly damaging	0.93
R5751:Vmn2r90	UTSW	17	17733866	missense	probably damaging	0.96
R6156:Vmn2r90	UTSW	17	17733344	missense	probably benign	0.01
R6185:Vmn2r90	UTSW	17	17733382	missense	probably damaging	1.00
R6450:Vmn2r90	UTSW	17	17733236	missense	possibly damaging	0.47
R6707:Vmn2r90	UTSW	17	17728102	missense	probably damaging	1.00
R6802:Vmn2r90	UTSW	17	17712089	missense	probably damaging	1.00
R6913:Vmn2r90	UTSW	17	17704061	missense	probably damaging	1.00
R7070:Vmn2r90	UTSW	17	17704051	missense	probably damaging	0.98
R7237:Vmn2r90	UTSW	17	17703987	missense	possibly damaging	0.92
R7503:Vmn2r90	UTSW	17	17713248	missense	not run
R7698:Vmn2r90	UTSW	17	17733334	missense	probably benign
R7943:Vmn2r90	UTSW	17	17712304	missense	probably damaging	1.00
R8072:Vmn2r90	UTSW	17	17726880	missense	probably damaging	1.00
R8128:Vmn2r90	UTSW	17	17733893	missense	probably damaging	1.00
R8295:Vmn2r90	UTSW	17	17728096	missense	probably benign	0.03
R8345:Vmn2r90	UTSW	17	17712865	nonsense	probably null
R8682:Vmn2r90	UTSW	17	17712082	missense	possibly damaging	0.95
R8716:Vmn2r90	UTSW	17	17704081	missense	probably damaging	0.98
R9041:Vmn2r90	UTSW	17	17734024	missense	probably benign	0.00
R9412:Vmn2r90	UTSW	17	17733951	missense	probably damaging	1.00
R9607:Vmn2r90	UTSW	17	17733376	missense	possibly damaging	0.88
R9705:Vmn2r90	UTSW	17	17712777	missense	possibly damaging	0.91
Z1088:Vmn2r90	UTSW	17	17733617	missense	probably damaging	1.00
Z1176:Vmn2r90	UTSW	17	17712817	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCATGAAGAGATGGCTGAATTTAC -3'
(R):5'- TTGCATTGACCTCAAGACAGGG -3'

Sequencing Primer
(F):5'- CATTTTCTGAGTTTGATTGCCAAC -3'
(R):5'- CATTGACCTCAAGACAGGGAAATAAG -3'

Posted On

2016-11-09