Mutagenetix > Incidental Mutation

Incidental Mutation 'R5662:Or1e25'

444147

Institutional Source

Beutler Lab

Gene Symbol

Or1e25

Ensembl Gene

ENSMUSG00000060335

Gene Name

olfactory receptor family 1 subfamily E member 25

Synonyms

GA_x6K02T2P1NL-3739520-3740032, Olfr384, Olfr386, GA_x6K02T2P1NL-3773152-3774090, MOR135-5

MMRRC Submission

043305-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.165) question?

Stock #

R5662 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

73493408-73494346 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 73494005 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Phenylalanine to Leucine at position 200 (F200L)

Ref Sequence

ENSEMBL: ENSMUSP00000148997 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000072993] [ENSMUST00000214228]

AlphaFold

Q7TRX8

Predicted Effect

probably benign
Transcript: ENSMUST00000072993
AA Change: F200L

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000072758
Gene: ENSMUSG00000060335
AA Change: F200L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.5e-60	PFAM
Pfam:7TM_GPCR_Srsx	35	305	5.6e-9	PFAM
Pfam:7tm_1	41	290	1.4e-25	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214228
AA Change: F200L

PolyPhen 2 Score 0.029 (Sensitivity: 0.95; Specificity: 0.82)

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.5%
20x: 95.8%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 49 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acan	T	A	7: 78,749,855 (GRCm39)	V1542D	possibly damaging	Het
Acsm4	T	G	7: 119,294,023 (GRCm39)	N131K	possibly damaging	Het
Ankrd54	A	G	15: 78,946,814 (GRCm39)	S62P	possibly damaging	Het
Ccdc186	A	G	19: 56,781,920 (GRCm39)	I753T	probably benign	Het
Cers2	C	T	3: 95,228,295 (GRCm39)	R112C	probably damaging	Het
Col6a4	C	G	9: 105,945,200 (GRCm39)	R971S	probably damaging	Het
Ctsf	T	C	19: 4,906,606 (GRCm39)	S178P	probably damaging	Het
Dennd4c	C	T	4: 86,713,525 (GRCm39)	T492I	probably benign	Het
Dhx8	A	G	11: 101,657,584 (GRCm39)	K1212E	possibly damaging	Het
Dnah8	A	G	17: 30,956,307 (GRCm39)	T2096A	probably damaging	Het
Ephb4	A	T	5: 137,370,457 (GRCm39)	I886F	probably damaging	Het
Eprs1	T	G	1: 185,126,622 (GRCm39)	N519K	possibly damaging	Het
Fam3c	T	C	6: 22,355,061 (GRCm39)		probably benign	Het
Galnt17	G	A	5: 131,114,844 (GRCm39)	R219C	probably damaging	Het
Gm10645	A	G	8: 83,892,486 (GRCm39)		probably benign	Het
Gpr132	G	A	12: 112,816,416 (GRCm39)	R137C	probably damaging	Het
Hydin	T	G	8: 111,307,341 (GRCm39)	S3907A	probably benign	Het
Ighv1-49	A	T	12: 115,019,027 (GRCm39)	V56E	probably damaging	Het
Igtp	A	G	11: 58,097,105 (GRCm39)	D92G	probably damaging	Het
Itga1	C	T	13: 115,122,707 (GRCm39)	V750I	probably benign	Het
Katnip	A	G	7: 125,441,875 (GRCm39)	R595G	probably benign	Het
Kif3c	T	A	12: 3,417,031 (GRCm39)	Y351N	probably damaging	Het
Klhdc1	T	A	12: 69,329,939 (GRCm39)	I356N	probably benign	Het
Lrrc34	T	C	3: 30,685,473 (GRCm39)	Y292C	probably benign	Het
Ly75	G	A	2: 60,182,725 (GRCm39)	T526M	probably damaging	Het
Mroh4	A	T	15: 74,497,277 (GRCm39)	D181E	possibly damaging	Het
Nutm1	T	C	2: 112,079,645 (GRCm39)	N757D	probably benign	Het
Or1m1	A	G	9: 18,666,896 (GRCm39)	F12L	probably damaging	Het
Or6c215	T	C	10: 129,638,176 (GRCm39)	T73A	possibly damaging	Het
Or9s18	T	C	13: 65,300,067 (GRCm39)	F10L	possibly damaging	Het
Paxbp1	G	A	16: 90,834,285 (GRCm39)	T167M	probably benign	Het
Pnpla7	A	G	2: 24,942,396 (GRCm39)	D1238G	probably damaging	Het
Pom121l2	T	C	13: 22,166,358 (GRCm39)	S210P	probably benign	Het
Ripor3	T	G	2: 167,835,476 (GRCm39)	N165T	probably benign	Het
Serpine3	T	G	14: 62,908,291 (GRCm39)	S106R	probably benign	Het
Slc25a11	G	A	11: 70,536,245 (GRCm39)	R158*	probably null	Het
Slc26a6	G	A	9: 108,736,538 (GRCm39)	V506M	possibly damaging	Het
Slc45a2	A	G	15: 11,022,169 (GRCm39)	K304E	probably benign	Het
Slc4a4	C	T	5: 89,176,103 (GRCm39)	L25F	probably damaging	Het
Smarcb1	T	C	10: 75,740,404 (GRCm39)	N267S	possibly damaging	Het
Spink6	C	A	18: 44,207,481 (GRCm39)	Q24K	possibly damaging	Het
Srebf2	T	C	15: 82,079,204 (GRCm39)	S811P	probably benign	Het
Ssc4d	A	G	5: 135,989,748 (GRCm39)	*587R	probably null	Het
Sult2a8	C	T	7: 14,161,765 (GRCm39)	R27H	probably benign	Het
Sun2	C	T	15: 79,623,069 (GRCm39)	A90T	probably benign	Het
Ubr7	T	C	12: 102,734,526 (GRCm39)	S267P	probably benign	Het
Wdr55	G	A	18: 36,893,448 (GRCm39)	V37M	possibly damaging	Het
Xdh	C	T	17: 74,248,110 (GRCm39)	G45S	probably damaging	Het
Zfp638	T	A	6: 83,920,111 (GRCm39)	S570T	probably damaging	Het

Other mutations in Or1e25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01476:Or1e25	APN	11	73,494,056 (GRCm39)	missense	probably damaging	1.00
IGL01767:Or1e25	APN	11	73,493,858 (GRCm39)	missense	probably benign
IGL02296:Or1e25	APN	11	73,493,532 (GRCm39)	missense	probably damaging	1.00
IGL02327:Or1e25	APN	11	73,493,981 (GRCm39)	missense	probably damaging	1.00
IGL02740:Or1e25	APN	11	73,493,657 (GRCm39)	missense	probably benign	0.41
H8562:Or1e25	UTSW	11	73,494,273 (GRCm39)	missense	probably damaging	1.00
R0594:Or1e25	UTSW	11	73,494,218 (GRCm39)	missense	probably benign	0.03
R2038:Or1e25	UTSW	11	73,494,239 (GRCm39)	missense	probably damaging	1.00
R2127:Or1e25	UTSW	11	73,493,631 (GRCm39)	missense	possibly damaging	0.78
R2844:Or1e25	UTSW	11	73,494,209 (GRCm39)	missense	probably benign	0.12
R2846:Or1e25	UTSW	11	73,494,209 (GRCm39)	missense	probably benign	0.12
R3877:Or1e25	UTSW	11	73,493,979 (GRCm39)	missense	probably damaging	1.00
R4193:Or1e25	UTSW	11	73,494,243 (GRCm39)	missense	probably damaging	1.00
R4433:Or1e25	UTSW	11	73,493,712 (GRCm39)	missense	probably damaging	1.00
R4824:Or1e25	UTSW	11	73,493,426 (GRCm39)	missense	possibly damaging	0.61
R4851:Or1e25	UTSW	11	73,493,883 (GRCm39)	missense	probably damaging	1.00
R5285:Or1e25	UTSW	11	73,493,767 (GRCm39)	nonsense	probably null
R5326:Or1e25	UTSW	11	73,494,030 (GRCm39)	missense	possibly damaging	0.94
R5542:Or1e25	UTSW	11	73,494,030 (GRCm39)	missense	possibly damaging	0.94
R6489:Or1e25	UTSW	11	73,494,265 (GRCm39)	missense	probably damaging	1.00
R6770:Or1e25	UTSW	11	73,493,804 (GRCm39)	missense	probably benign	0.04
R7131:Or1e25	UTSW	11	73,493,562 (GRCm39)	missense	possibly damaging	0.78
R7313:Or1e25	UTSW	11	73,493,810 (GRCm39)	missense	probably damaging	0.99
R7754:Or1e25	UTSW	11	73,494,332 (GRCm39)	nonsense	probably null
R8393:Or1e25	UTSW	11	73,494,261 (GRCm39)	missense	probably damaging	1.00
R9190:Or1e25	UTSW	11	73,493,877 (GRCm39)	missense	probably benign	0.36
R9628:Or1e25	UTSW	11	73,493,864 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCTATGTGGCCATCTGCTTC -3'
(R):5'- TAGCTGATGGGCATAAGTAGACAC -3'

Sequencing Primer
(F):5'- TCTTCATTACACCAGCATCATGAG -3'
(R):5'- CCAATAATTGTCCCATAGAACAGTG -3'

Posted On

2016-11-09