Mutagenetix > Incidental Mutation

Incidental Mutation 'R5664:Myo9b'

444270

Institutional Source

Beutler Lab

Gene Symbol

Myo9b

Ensembl Gene

ENSMUSG00000004677

Gene Name

myosin IXb

Synonyms

MMRRC Submission

043307-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.770) question?

Stock #

R5664 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

71272714-71360713 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 71359882 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 2099 (D2099G)

Ref Sequence

ENSEMBL: ENSMUSP00000129220 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000071935] [ENSMUST00000168839] [ENSMUST00000170242] [ENSMUST00000212935]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000071935

SMART Domains

Protein: ENSMUSP00000071827
Gene: ENSMUSG00000004677

Domain	Start	End	E-Value	Type
RA	15	114	3.7e-30	SMART
MYSc	140	954	N/A	SMART
IQ	955	977	1.2e-3	SMART
IQ	978	1000	1.6e-5	SMART
IQ	1001	1022	4.3e-5	SMART
IQ	1023	1045	8.4e-5	SMART
low complexity region	1050	1064	N/A	INTRINSIC
low complexity region	1127	1145	N/A	INTRINSIC
low complexity region	1211	1222	N/A	INTRINSIC
low complexity region	1232	1246	N/A	INTRINSIC
Blast:MYSc	1247	1323	3e-19	BLAST
low complexity region	1348	1359	N/A	INTRINSIC
coiled coil region	1563	1590	N/A	INTRINSIC
C1	1591	1639	1.7e-14	SMART
RhoGAP	1668	1843	4.7e-71	SMART
coiled coil region	1901	1925	N/A	INTRINSIC
low complexity region	1940	1952	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000168839

SMART Domains

Protein: ENSMUSP00000131635
Gene: ENSMUSG00000004677

Domain	Start	End	E-Value	Type
RA	15	114	5.79e-28	SMART
MYSc	140	954	N/A	SMART
IQ	955	977	2.46e-1	SMART
IQ	978	1000	3.35e-3	SMART
IQ	1001	1022	8.84e-3	SMART
IQ	1023	1045	1.77e-2	SMART
low complexity region	1050	1064	N/A	INTRINSIC
low complexity region	1127	1145	N/A	INTRINSIC
low complexity region	1211	1222	N/A	INTRINSIC
low complexity region	1234	1257	N/A	INTRINSIC
Blast:MYSc	1258	1334	3e-19	BLAST
low complexity region	1361	1372	N/A	INTRINSIC
low complexity region	1581	1601	N/A	INTRINSIC
C1	1605	1653	3.58e-12	SMART
RhoGAP	1682	1857	7.78e-69	SMART
coiled coil region	1915	1939	N/A	INTRINSIC
low complexity region	1954	1966	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000170242
AA Change: D2099G

PolyPhen 2 Score 0.134 (Sensitivity: 0.92; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000129220
Gene: ENSMUSG00000004677
AA Change: D2099G

Domain	Start	End	E-Value	Type
RA	15	114	5.79e-28	SMART
MYSc	140	954	N/A	SMART
IQ	955	977	2.46e-1	SMART
IQ	978	1000	3.35e-3	SMART
IQ	1001	1022	8.84e-3	SMART
IQ	1023	1045	1.77e-2	SMART
low complexity region	1050	1064	N/A	INTRINSIC
low complexity region	1127	1145	N/A	INTRINSIC
low complexity region	1211	1222	N/A	INTRINSIC
low complexity region	1234	1257	N/A	INTRINSIC
Blast:MYSc	1258	1334	3e-19	BLAST
low complexity region	1361	1372	N/A	INTRINSIC
low complexity region	1581	1601	N/A	INTRINSIC
C1	1605	1653	3.58e-12	SMART
RhoGAP	1682	1857	7.78e-69	SMART
coiled coil region	1931	1955	N/A	INTRINSIC
low complexity region	1970	1982	N/A	INTRINSIC
low complexity region	1992	2003	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000212412

Predicted Effect

probably benign
Transcript: ENSMUST00000212935

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.6%
20x: 96.0%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the myosin family of actin-based molecular motor heavy chain proteins. The protein represents an unconventional myosin; it should not be confused with the conventional non-muscle myosin-9 (MYH9). The protein has four IQ motifs located in the neck domain that bind calmodulin, which serves as a light chain. The protein complex has a single-headed structure and exhibits processive movement on actin filaments toward the minus-end. The protein also has rho-GTPase activity. Polymorphisms in this gene are associated with celiac disease and ulcerative colitis susceptibility. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
PHENOTYPE: Homozygous null mutants breed normal, but shows defect in macrophage motility and chemotaxis. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610030E20Rik	T	A	6: 72,348,994 (GRCm38)		probably null	Het
1810032O08Rik	A	T	11: 116,672,664 (GRCm38)	T27S	possibly damaging	Het
4930432K21Rik	A	T	8: 84,166,659 (GRCm38)	I152F	probably benign	Het
9430015G10Rik	T	A	4: 156,123,559 (GRCm38)	L112H	probably damaging	Het
Acaca	T	C	11: 84,243,384 (GRCm38)	L441P	probably damaging	Het
Ank3	G	A	10: 70,002,565 (GRCm38)	R1566K	possibly damaging	Het
Arsj	A	T	3: 126,438,657 (GRCm38)	I351F	probably damaging	Het
Atp6v1b2	A	G	8: 69,107,620 (GRCm38)	T373A	probably damaging	Het
Atr	C	A	9: 95,905,813 (GRCm38)	N1486K	probably benign	Het
Avl9	T	C	6: 56,753,839 (GRCm38)	S583P	probably damaging	Het
Bptf	A	T	11: 107,073,699 (GRCm38)	D1493E	probably benign	Het
C2cd2l	T	C	9: 44,313,772 (GRCm38)	E548G	probably damaging	Het
Capn3	G	A	2: 120,477,025 (GRCm38)	R15Q	probably benign	Het
Ccl3	A	G	11: 83,649,213 (GRCm38)	F22S	probably benign	Het
Clcf1	T	C	19: 4,222,096 (GRCm38)	F69S	probably damaging	Het
Col13a1	T	C	10: 61,851,116 (GRCm38)	E170G	probably damaging	Het
Dhx29	T	A	13: 112,946,879 (GRCm38)	F489L	probably damaging	Het
Dhx8	A	T	11: 101,740,751 (GRCm38)	N390I	probably damaging	Het
Dkk1	T	A	19: 30,548,789 (GRCm38)	Y135F	probably benign	Het
Edil3	G	T	13: 89,319,713 (GRCm38)	V446F	probably damaging	Het
Epha5	T	A	5: 84,331,866 (GRCm38)	E93V	probably damaging	Het
Epsti1	C	T	14: 77,963,664 (GRCm38)	T196I	possibly damaging	Het
Fras1	T	C	5: 96,728,535 (GRCm38)	S2376P	possibly damaging	Het
Frem2	A	G	3: 53,652,490 (GRCm38)	V1532A	probably benign	Het
Fsip2	T	A	2: 82,988,095 (GRCm38)	M4724K	probably benign	Het
Gcat	T	A	15: 79,043,073 (GRCm38)	L238Q	probably damaging	Het
Gimap6	T	C	6: 48,702,275 (GRCm38)	K276E	probably benign	Het
Gjb5	T	A	4: 127,355,929 (GRCm38)	I141F	probably benign	Het
Glt6d1	T	C	2: 25,814,180 (GRCm38)	I7V	probably benign	Het
Gm37596	A	G	3: 93,692,687 (GRCm38)	F125S	probably benign	Het
Gm5771	C	T	6: 41,394,671 (GRCm38)	P17L	probably benign	Het
Gm6169	C	A	13: 97,099,121 (GRCm38)	L39F	probably damaging	Het
Gtf2h5	G	A	17: 6,084,524 (GRCm38)	G30R	probably damaging	Het
Herc6	C	A	6: 57,618,684 (GRCm38)	T449K	probably benign	Het
Hpn	A	T	7: 31,099,262 (GRCm38)	Y132N	probably damaging	Het
Hpx	A	T	7: 105,595,148 (GRCm38)	M190K	probably benign	Het
Inf2	A	G	12: 112,611,728 (GRCm38)	H1151R	unknown	Het
Krt74	A	G	15: 101,760,579 (GRCm38)		noncoding transcript	Het
Loxl3	G	A	6: 83,049,882 (GRCm38)	S564N	probably benign	Het
Map7	T	A	10: 20,267,359 (GRCm38)	V418E	unknown	Het
Mrpl37	T	C	4: 107,064,391 (GRCm38)	N214D	probably benign	Het
Mthfr	T	C	4: 148,055,466 (GRCm38)	Y656H	probably damaging	Het
Nktr	T	A	9: 121,749,417 (GRCm38)	C825*	probably null	Het
Nomo1	A	G	7: 46,076,157 (GRCm38)	E1029G	probably benign	Het
Nup133	T	C	8: 123,906,281 (GRCm38)	D1037G	probably benign	Het
Olfr1271	A	G	2: 90,265,615 (GRCm38)	F272L	probably damaging	Het
Olfr153	T	C	2: 87,532,834 (GRCm38)	L267P	probably benign	Het
Pcdhb14	T	A	18: 37,448,996 (GRCm38)	V385D	possibly damaging	Het
Pik3c2g	T	C	6: 139,737,007 (GRCm38)	L38P	probably damaging	Het
Pkd1	A	T	17: 24,569,371 (GRCm38)	D701V	probably damaging	Het
Pnpla6	A	G	8: 3,537,478 (GRCm38)	T1070A	probably damaging	Het
Ppl	T	C	16: 5,106,055 (GRCm38)	D185G	probably benign	Het
Prune1	A	T	3: 95,258,178 (GRCm38)	L261Q	probably damaging	Het
Qser1	A	T	2: 104,778,196 (GRCm38)	L1444I	probably damaging	Het
Serpina6	A	C	12: 103,654,467 (GRCm38)	C8G	probably damaging	Het
Sla2	A	T	2: 156,874,999 (GRCm38)	D180E	probably benign	Het
Slc4a4	C	T	5: 89,028,244 (GRCm38)	L25F	probably damaging	Het
Tbx3	A	G	5: 119,678,731 (GRCm38)	K311R	possibly damaging	Het
Thbs2	A	T	17: 14,689,837 (GRCm38)	C167S	probably damaging	Het
Trak1	T	A	9: 121,472,307 (GRCm38)	C710S	possibly damaging	Het
Tsks	G	A	7: 44,953,784 (GRCm38)	E337K	probably damaging	Het
Vcpip1	A	G	1: 9,746,379 (GRCm38)	I593T	probably damaging	Het
Vmn2r118	A	G	17: 55,592,765 (GRCm38)	I713T	possibly damaging	Het
Vmn2r23	C	T	6: 123,713,074 (GRCm38)	T303M	probably damaging	Het
Vmn2r68	A	T	7: 85,233,770 (GRCm38)	M258K	probably benign	Het
Vmn2r76	A	G	7: 86,245,994 (GRCm38)		probably null	Het
Wap	A	G	11: 6,638,609 (GRCm38)	I5T	possibly damaging	Het
Zfp235	A	C	7: 24,142,151 (GRCm38)	H665P	probably damaging	Het

Other mutations in Myo9b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00163:Myo9b	APN	8	71,348,735 (GRCm38)	missense	probably benign
IGL01020:Myo9b	APN	8	71,352,000 (GRCm38)	missense	probably benign
IGL01479:Myo9b	APN	8	71,359,342 (GRCm38)	missense	probably damaging	1.00
IGL01704:Myo9b	APN	8	71,359,642 (GRCm38)	missense	probably damaging	0.98
IGL01761:Myo9b	APN	8	71,349,152 (GRCm38)	missense	probably damaging	0.96
IGL01766:Myo9b	APN	8	71,290,517 (GRCm38)	missense	probably damaging	1.00
IGL01834:Myo9b	APN	8	71,355,257 (GRCm38)	missense	possibly damaging	0.93
IGL01834:Myo9b	APN	8	71,356,318 (GRCm38)	missense	probably damaging	1.00
IGL01838:Myo9b	APN	8	71,334,390 (GRCm38)	missense	probably damaging	0.99
IGL02318:Myo9b	APN	8	71,354,124 (GRCm38)	missense	probably damaging	0.98
IGL02333:Myo9b	APN	8	71,358,993 (GRCm38)	missense	possibly damaging	0.65
IGL02340:Myo9b	APN	8	71,291,045 (GRCm38)	missense	probably damaging	1.00
IGL02514:Myo9b	APN	8	71,291,006 (GRCm38)	missense	probably damaging	1.00
IGL02593:Myo9b	APN	8	71,290,773 (GRCm38)	missense	probably damaging	1.00
IGL03075:Myo9b	APN	8	71,354,527 (GRCm38)	missense	probably damaging	1.00
IGL03332:Myo9b	APN	8	71,348,774 (GRCm38)	missense	possibly damaging	0.78
avantgarde	UTSW	8	71,344,162 (GRCm38)	missense	probably damaging	1.00
Freaky	UTSW	8	71,290,819 (GRCm38)	missense	probably damaging	1.00
iconoclastic	UTSW	8	71,290,475 (GRCm38)	missense	probably benign	0.37
unconventional	UTSW	8	71,348,597 (GRCm38)	missense	probably benign	0.00
PIT4418001:Myo9b	UTSW	8	71,322,947 (GRCm38)	missense	probably damaging	1.00
PIT4651001:Myo9b	UTSW	8	71,342,812 (GRCm38)	missense	possibly damaging	0.83
R0023:Myo9b	UTSW	8	71,333,768 (GRCm38)	missense	probably damaging	1.00
R0103:Myo9b	UTSW	8	71,323,849 (GRCm38)	splice site	probably benign
R0103:Myo9b	UTSW	8	71,323,849 (GRCm38)	splice site	probably benign
R0144:Myo9b	UTSW	8	71,346,043 (GRCm38)	missense	probably damaging	1.00
R0207:Myo9b	UTSW	8	71,355,225 (GRCm38)	splice site	probably benign
R0226:Myo9b	UTSW	8	71,353,832 (GRCm38)	missense	probably damaging	1.00
R0227:Myo9b	UTSW	8	71,344,162 (GRCm38)	missense	probably damaging	1.00
R0244:Myo9b	UTSW	8	71,321,813 (GRCm38)	missense	probably damaging	1.00
R0277:Myo9b	UTSW	8	71,355,952 (GRCm38)	splice site	probably benign
R0362:Myo9b	UTSW	8	71,347,770 (GRCm38)	missense	probably damaging	1.00
R0689:Myo9b	UTSW	8	71,330,756 (GRCm38)	missense	probably damaging	1.00
R0844:Myo9b	UTSW	8	71,290,475 (GRCm38)	missense	probably benign	0.37
R1051:Myo9b	UTSW	8	71,355,822 (GRCm38)	missense	probably damaging	1.00
R1469:Myo9b	UTSW	8	71,291,036 (GRCm38)	missense	probably damaging	1.00
R1469:Myo9b	UTSW	8	71,291,036 (GRCm38)	missense	probably damaging	1.00
R1526:Myo9b	UTSW	8	71,355,764 (GRCm38)	missense	probably damaging	1.00
R1544:Myo9b	UTSW	8	71,290,976 (GRCm38)	missense	probably damaging	1.00
R1565:Myo9b	UTSW	8	71,315,192 (GRCm38)	missense	possibly damaging	0.46
R1645:Myo9b	UTSW	8	71,322,978 (GRCm38)	missense	probably damaging	1.00
R1745:Myo9b	UTSW	8	71,354,047 (GRCm38)	missense	probably damaging	1.00
R1820:Myo9b	UTSW	8	71,333,358 (GRCm38)	missense	probably damaging	1.00
R2037:Myo9b	UTSW	8	71,290,866 (GRCm38)	missense	probably damaging	1.00
R2050:Myo9b	UTSW	8	71,290,550 (GRCm38)	missense	probably damaging	1.00
R2056:Myo9b	UTSW	8	71,359,690 (GRCm38)	missense	possibly damaging	0.78
R2129:Myo9b	UTSW	8	71,333,699 (GRCm38)	missense	probably damaging	1.00
R2423:Myo9b	UTSW	8	71,327,940 (GRCm38)	missense	probably damaging	1.00
R2869:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2869:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2871:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2871:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2872:Myo9b	UTSW	8	71,290,966 (GRCm38)	missense	probably benign	0.01
R2872:Myo9b	UTSW	8	71,290,966 (GRCm38)	missense	probably benign	0.01
R2873:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2874:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2920:Myo9b	UTSW	8	71,325,857 (GRCm38)	missense	probably damaging	0.98
R2926:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2939:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R2940:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R3033:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R3040:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R3689:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R3691:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R3735:Myo9b	UTSW	8	71,348,597 (GRCm38)	missense	probably benign	0.00
R4194:Myo9b	UTSW	8	71,359,624 (GRCm38)	missense	possibly damaging	0.71
R4258:Myo9b	UTSW	8	71,355,765 (GRCm38)	missense	probably damaging	1.00
R4457:Myo9b	UTSW	8	71,290,999 (GRCm38)	missense	probably damaging	1.00
R4478:Myo9b	UTSW	8	71,291,081 (GRCm38)	missense	probably damaging	1.00
R4496:Myo9b	UTSW	8	71,334,337 (GRCm38)	missense	probably benign	0.01
R4544:Myo9b	UTSW	8	71,327,941 (GRCm38)	missense	probably damaging	1.00
R4580:Myo9b	UTSW	8	71,315,135 (GRCm38)	missense	probably damaging	1.00
R4736:Myo9b	UTSW	8	71,356,592 (GRCm38)	missense	probably damaging	1.00
R5068:Myo9b	UTSW	8	71,349,055 (GRCm38)	missense	probably damaging	1.00
R5124:Myo9b	UTSW	8	71,355,839 (GRCm38)	missense	probably damaging	1.00
R5194:Myo9b	UTSW	8	71,349,089 (GRCm38)	missense	probably benign	0.01
R5296:Myo9b	UTSW	8	71,333,388 (GRCm38)	missense	possibly damaging	0.69
R5528:Myo9b	UTSW	8	71,323,274 (GRCm38)	missense	probably benign	0.06
R5677:Myo9b	UTSW	8	71,343,686 (GRCm38)	missense	probably damaging	1.00
R5680:Myo9b	UTSW	8	71,290,372 (GRCm38)	missense	probably benign	0.00
R5982:Myo9b	UTSW	8	71,348,396 (GRCm38)	missense	probably benign	0.05
R6344:Myo9b	UTSW	8	71,327,914 (GRCm38)	missense	probably damaging	1.00
R6352:Myo9b	UTSW	8	71,348,411 (GRCm38)	missense	probably benign
R6352:Myo9b	UTSW	8	71,348,410 (GRCm38)	missense	probably benign	0.16
R6411:Myo9b	UTSW	8	71,322,955 (GRCm38)	nonsense	probably null
R6425:Myo9b	UTSW	8	71,333,628 (GRCm38)	missense	probably damaging	1.00
R6505:Myo9b	UTSW	8	71,355,857 (GRCm38)	missense	possibly damaging	0.88
R6743:Myo9b	UTSW	8	71,352,159 (GRCm38)	splice site	probably null
R6811:Myo9b	UTSW	8	71,356,578 (GRCm38)	missense	probably damaging	1.00
R6813:Myo9b	UTSW	8	71,323,305 (GRCm38)	missense	probably damaging	1.00
R6954:Myo9b	UTSW	8	71,290,819 (GRCm38)	missense	probably damaging	1.00
R7124:Myo9b	UTSW	8	71,333,701 (GRCm38)	nonsense	probably null
R7255:Myo9b	UTSW	8	71,290,891 (GRCm38)	missense	probably damaging	1.00
R7293:Myo9b	UTSW	8	71,325,905 (GRCm38)	missense	probably benign	0.00
R7342:Myo9b	UTSW	8	71,355,774 (GRCm38)	missense	probably damaging	1.00
R7451:Myo9b	UTSW	8	71,352,188 (GRCm38)	missense	probably benign	0.28
R7482:Myo9b	UTSW	8	71,342,798 (GRCm38)	missense	probably benign	0.00
R7508:Myo9b	UTSW	8	71,354,801 (GRCm38)	missense	probably benign	0.00
R7957:Myo9b	UTSW	8	71,354,761 (GRCm38)	missense	probably benign	0.12
R8062:Myo9b	UTSW	8	71,321,813 (GRCm38)	missense	probably damaging	0.99
R8108:Myo9b	UTSW	8	71,348,342 (GRCm38)	missense	probably damaging	0.99
R8197:Myo9b	UTSW	8	71,290,963 (GRCm38)	missense	probably damaging	1.00
R8274:Myo9b	UTSW	8	71,359,836 (GRCm38)	missense	probably benign	0.00
R8686:Myo9b	UTSW	8	71,334,322 (GRCm38)	missense	probably benign	0.01
R8731:Myo9b	UTSW	8	71,353,842 (GRCm38)	critical splice donor site	probably null
R8924:Myo9b	UTSW	8	71,349,031 (GRCm38)	missense	probably benign
R9056:Myo9b	UTSW	8	71,352,262 (GRCm38)	missense	probably benign	0.17
R9117:Myo9b	UTSW	8	71,347,807 (GRCm38)	missense	probably benign	0.03
R9151:Myo9b	UTSW	8	71,355,227 (GRCm38)	splice site	probably benign
R9315:Myo9b	UTSW	8	71,349,167 (GRCm38)	missense	possibly damaging	0.54
R9332:Myo9b	UTSW	8	71,359,602 (GRCm38)	missense	probably benign	0.07
R9364:Myo9b	UTSW	8	71,355,839 (GRCm38)	missense	probably damaging	1.00
R9569:Myo9b	UTSW	8	71,358,985 (GRCm38)	missense	probably benign
R9581:Myo9b	UTSW	8	71,359,899 (GRCm38)	missense	probably benign	0.19
R9600:Myo9b	UTSW	8	71,290,431 (GRCm38)	missense	possibly damaging	0.80
X0066:Myo9b	UTSW	8	71,323,898 (GRCm38)	missense	probably damaging	1.00
Z1177:Myo9b	UTSW	8	71,290,709 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACGGTCAGAGTGAAGACACC -3'
(R):5'- CTTCTCAGAAAGTGAAGGCCAG -3'

Sequencing Primer
(F):5'- TGAAGACACCTCGGAGGACC -3'
(R):5'- AGTGAAGGCCAGCTCCACAG -3'

Posted On

2016-11-09