Mutagenetix > Incidental Mutation

Incidental Mutation 'R5667:Ifna6'

444464

Institutional Source

Beutler Lab

Gene Symbol

Ifna6

Ensembl Gene

ENSMUSG00000101252

Gene Name

interferon alpha 6

Synonyms

Ifa6, Ifna8

MMRRC Submission

043310-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R5667 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

88745653-88746222 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 88745906 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamine to Leucine at position 85 (Q85L)

Ref Sequence

ENSEMBL: ENSMUSP00000100777 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000105146]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000105146
AA Change: Q85L

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000100777
Gene: ENSMUSG00000101252
AA Change: Q85L

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
IFabd	58	175	5.8e-71	SMART

Meta Mutation Damage Score

0.5324

Coding Region Coverage

1x: 99.4%
3x: 98.8%
10x: 97.6%
20x: 96.0%

Validation Efficiency

96% (54/56)

MGI Phenotype

PHENOTYPE: Mice heterozygous for reporter allele are viable and fertile, and show normal production of interferon-alpha in response to viral infection. [provided by MGI curators]

Allele List at MGI

All alleles(1) : Targeted(1)

Other mutations in this stock

Total: 53 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2700049A03Rik	A	T	12: 71,211,321 (GRCm39)	E685V	possibly damaging	Het
2700049A03Rik	G	T	12: 71,211,320 (GRCm39)	E685*	probably null	Het
Adamts16	A	T	13: 70,984,494 (GRCm39)	Y56*	probably null	Het
Ak2	T	A	4: 128,902,040 (GRCm39)	F238I	probably damaging	Het
Akap8l	T	C	17: 32,557,266 (GRCm39)	Y115C	probably damaging	Het
Alms1	A	G	6: 85,673,753 (GRCm39)	D3116G	probably damaging	Het
Arhgap24	T	G	5: 102,994,037 (GRCm39)		probably null	Het
Arhgap45	A	G	10: 79,861,310 (GRCm39)	E491G	probably damaging	Het
Atp8b1	C	T	18: 64,714,994 (GRCm39)	C86Y	probably damaging	Het
Atxn1	T	C	13: 45,710,853 (GRCm39)	K693R	probably benign	Het
Bltp1	A	T	3: 36,971,826 (GRCm39)	T520S	probably benign	Het
Btbd10	T	C	7: 112,931,931 (GRCm39)	K165R	probably damaging	Het
Capn11	C	T	17: 45,950,600 (GRCm39)	R293Q	possibly damaging	Het
Chordc1	T	G	9: 18,206,628 (GRCm39)	F33V	probably damaging	Het
Clca4b	G	A	3: 144,627,624 (GRCm39)	T449I	probably benign	Het
Clip4	A	C	17: 72,096,878 (GRCm39)	M1L	probably damaging	Het
Cyfip1	C	T	7: 55,523,478 (GRCm39)	T90I	probably benign	Het
Cyp4v3	G	T	8: 45,761,572 (GRCm39)	T417K	possibly damaging	Het
Exoc3l4	T	C	12: 111,389,851 (GRCm39)	I142T	probably damaging	Het
Flnb	T	A	14: 7,890,843 (GRCm38)	I575N	probably benign	Het
Foxa1	A	T	12: 57,589,081 (GRCm39)	S380T	probably benign	Het
Foxi2	A	T	7: 135,012,668 (GRCm39)		probably null	Het
Gad1-ps	A	T	10: 99,280,395 (GRCm39)		noncoding transcript	Het
Gpa33	A	G	1: 165,974,360 (GRCm39)	T66A	possibly damaging	Het
Gpr45	A	G	1: 43,072,218 (GRCm39)	Y287C	probably damaging	Het
H2-Eb1	C	A	17: 34,533,229 (GRCm39)	Y150*	probably null	Het
Hsd17b8	T	C	17: 34,245,435 (GRCm39)	D233G	probably null	Het
Ivns1abp	G	T	1: 151,229,760 (GRCm39)	L149F	probably benign	Het
Kank4	A	G	4: 98,653,698 (GRCm39)		probably null	Het
Katnip	T	A	7: 125,442,627 (GRCm39)		probably null	Het
Lama2	A	T	10: 27,066,540 (GRCm39)	C1114S	probably damaging	Het
Lmbr1l	A	C	15: 98,805,489 (GRCm39)	D337E	possibly damaging	Het
Lrrn3	A	C	12: 41,502,297 (GRCm39)	S673R	possibly damaging	Het
Ly75	T	C	2: 60,138,655 (GRCm39)	D1404G	probably damaging	Het
Muc4	A	G	16: 32,575,011 (GRCm39)	T1199A	probably benign	Het
Mycn	A	T	12: 12,990,045 (GRCm39)	M117K	possibly damaging	Het
Nalcn	A	G	14: 123,532,818 (GRCm39)	I1314T	probably damaging	Het
Nod1	T	C	6: 54,910,561 (GRCm39)	T869A	probably benign	Het
Or2y16	T	C	11: 49,335,140 (GRCm39)	V154A	probably benign	Het
Or4k39	C	T	2: 111,238,818 (GRCm39)		noncoding transcript	Het
Plekhg2	A	T	7: 28,067,064 (GRCm39)	I356N	probably damaging	Het
Ptpru	T	A	4: 131,547,501 (GRCm39)	Y112F	possibly damaging	Het
Rpl36al	G	A	12: 69,229,897 (GRCm39)	P5L	possibly damaging	Het
Ryr2	A	G	13: 11,774,722 (GRCm39)	W1145R	probably damaging	Het
Semp2l2b	T	A	10: 21,942,742 (GRCm39)	T413S	possibly damaging	Het
Slc11a2	A	G	15: 100,301,169 (GRCm39)	Y295H	probably damaging	Het
Slc22a6	A	G	19: 8,599,148 (GRCm39)		probably null	Het
Styxl1	A	G	5: 135,785,977 (GRCm39)		probably null	Het
Tmc6	A	G	11: 117,666,441 (GRCm39)	S288P	possibly damaging	Het
Trpv4	A	G	5: 114,772,617 (GRCm39)	L371P	probably damaging	Het
Uqcc4	G	A	17: 25,403,963 (GRCm39)	S101N	probably damaging	Het
Usp8	A	G	2: 126,584,345 (GRCm39)	D518G	probably benign	Het
Washc4	T	C	10: 83,405,892 (GRCm39)	S463P	probably damaging	Het

Other mutations in Ifna6

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL03403:Ifna6	APN	4	88,745,695 (GRCm39)	missense	possibly damaging	0.69
R0336:Ifna6	UTSW	4	88,746,178 (GRCm39)	missense	probably damaging	0.97
R0521:Ifna6	UTSW	4	88,745,887 (GRCm39)	missense	probably benign	0.30
R2863:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R2863:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R2865:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R2865:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R4171:Ifna6	UTSW	4	88,746,038 (GRCm39)	missense	probably benign	0.06
R4534:Ifna6	UTSW	4	88,746,099 (GRCm39)	missense	probably benign	0.36
R4534:Ifna6	UTSW	4	88,746,086 (GRCm39)	missense	probably benign
R4992:Ifna6	UTSW	4	88,745,777 (GRCm39)	missense	probably benign	0.00
R5671:Ifna6	UTSW	4	88,745,906 (GRCm39)	missense	probably damaging	1.00
R5677:Ifna6	UTSW	4	88,745,956 (GRCm39)	missense	probably benign	0.00
R6487:Ifna6	UTSW	4	88,745,743 (GRCm39)	missense	probably damaging	1.00
R6751:Ifna6	UTSW	4	88,745,987 (GRCm39)	missense	probably damaging	1.00
R7424:Ifna6	UTSW	4	88,746,044 (GRCm39)	missense	possibly damaging	0.53
R7711:Ifna6	UTSW	4	88,745,971 (GRCm39)	missense	probably benign	0.06
R8850:Ifna6	UTSW	4	88,746,222 (GRCm39)	makesense	probably null

Predicted Primers

PCR Primer
(F):5'- ATGTGACCTGCCTCAGACTC -3'
(R):5'- CTCTCTCAGGTACACAGTGATC -3'

Sequencing Primer
(F):5'- TGTGACCTGCCTCAGACTCATAAC -3'
(R):5'- ATCCTGTGGAAGTATGTCCTCACAG -3'

Posted On

2016-11-09