Mutagenetix > Incidental Mutation

Incidental Mutation 'R5759:Zfp1010'

445175

Institutional Source

Beutler Lab

Gene Symbol

Zfp1010

Ensembl Gene

ENSMUSG00000078869

Gene Name

zinc finger protein 1010

Synonyms

Gm14409

MMRRC Submission

043361-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R5759 (G1)

Quality Score

200

Status

Not validated

Chromosome

Chromosomal Location

176956447-176958829 bp(-) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

A to T at 176956765 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 244 (C244*)

Ref Sequence

ENSEMBL: ENSMUSP00000104591 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000108963]

AlphaFold

A2ARR8

Predicted Effect

probably null
Transcript: ENSMUST00000108963
AA Change: C244*

SMART Domains

Protein: ENSMUSP00000104591
Gene: ENSMUSG00000078869
AA Change: C244*

Domain	Start	End	E-Value	Type
Blast:KRAB	1	34	3e-14	BLAST
ZnF_C2H2	46	65	1.31e2	SMART
ZnF_C2H2	71	93	8.47e-4	SMART
ZnF_C2H2	99	121	5.99e-4	SMART
ZnF_C2H2	127	149	5.59e-4	SMART
ZnF_C2H2	155	177	2.53e-2	SMART
ZnF_C2H2	183	205	1.72e-4	SMART
ZnF_C2H2	211	233	2.12e-4	SMART
ZnF_C2H2	239	261	1.58e-3	SMART
ZnF_C2H2	267	289	1.03e-2	SMART

Coding Region Coverage

1x: 99.5%
3x: 98.8%
10x: 97.2%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	T	C	3: 137,774,419 (GRCm39)	S1203P	probably benign	Het
Adamts1	A	G	16: 85,594,936 (GRCm39)	V341A	possibly damaging	Het
Arf2	G	A	11: 103,874,459 (GRCm39)	G144S	probably benign	Het
Atr	A	G	9: 95,756,455 (GRCm39)	N862D	probably benign	Het
Cep295nl	T	C	11: 118,224,472 (GRCm39)	H124R	possibly damaging	Het
Chd6	C	T	2: 160,825,682 (GRCm39)	V1141I	possibly damaging	Het
Chst5	C	A	8: 112,616,842 (GRCm39)	K259N	probably benign	Het
Dchs1	T	A	7: 105,413,383 (GRCm39)	D1144V	probably damaging	Het
Dmxl2	T	C	9: 54,282,792 (GRCm39)	Y2795C	probably damaging	Het
Dnah7b	A	G	1: 46,316,280 (GRCm39)	N3131S	probably damaging	Het
Dnah7c	G	A	1: 46,654,527 (GRCm39)	G1436R	probably damaging	Het
Emp2	T	A	16: 10,102,374 (GRCm39)	Y146F	probably damaging	Het
Exoc6	C	T	19: 37,562,189 (GRCm39)	Q148*	probably null	Het
Fam13b	T	C	18: 34,630,488 (GRCm39)	D90G	probably damaging	Het
Fam186b	T	G	15: 99,177,598 (GRCm39)	Y576S	probably benign	Het
Fras1	T	C	5: 96,857,775 (GRCm39)	V2023A	probably benign	Het
Grm5	A	C	7: 87,675,808 (GRCm39)	M441L	probably damaging	Het
Hhatl	A	G	9: 121,617,343 (GRCm39)	Y297H	probably damaging	Het
Ifi30	A	G	8: 71,219,188 (GRCm39)		probably benign	Het
Ing2	G	T	8: 48,122,040 (GRCm39)	N169K	possibly damaging	Het
Kat6a	T	G	8: 23,428,028 (GRCm39)	S1128A	probably benign	Het
Madd	T	C	2: 90,992,420 (GRCm39)	E1041G	possibly damaging	Het
Mcm3	CT	CTT	1: 20,878,972 (GRCm39)		probably null	Het
Mfsd5	G	A	15: 102,189,513 (GRCm39)	G295D	possibly damaging	Het
Minar1	A	T	9: 89,483,125 (GRCm39)	N757K	probably benign	Het
Mmp20	T	A	9: 7,628,378 (GRCm39)		probably null	Het
Mybphl	T	C	3: 108,282,070 (GRCm39)	V100A	probably benign	Het
Ndfip2	T	A	14: 105,539,750 (GRCm39)		probably null	Het
Or7e170	A	G	9: 19,795,484 (GRCm39)	V39A	probably benign	Het
Or7e175	T	A	9: 20,049,228 (GRCm39)	V272E	probably benign	Het
Or8b1c	T	A	9: 38,384,831 (GRCm39)	S263T	possibly damaging	Het
Phf19	T	G	2: 34,787,135 (GRCm39)	D443A	probably damaging	Het
Piezo1	A	G	8: 123,234,394 (GRCm39)	V84A	probably damaging	Het
Septin9	A	G	11: 117,243,094 (GRCm39)	I94V	probably benign	Het
Slc35a1	C	A	4: 34,675,032 (GRCm39)	V132L	probably benign	Het
Sntg1	G	A	1: 8,484,494 (GRCm39)	S442L	probably benign	Het
Tcf25	A	G	8: 124,108,196 (GRCm39)	T84A	probably benign	Het
Tchhl1	C	G	3: 93,378,863 (GRCm39)	S522R	probably damaging	Het
Tmem106b	A	G	6: 13,075,041 (GRCm39)	E76G	probably damaging	Het
Tnk2	C	T	16: 32,499,482 (GRCm39)	P932S	probably benign	Het
Trib1	G	A	15: 59,526,350 (GRCm39)	V307I	probably benign	Het
Trib3	A	T	2: 152,185,215 (GRCm39)	D11E	probably benign	Het
Trim34b	T	C	7: 103,980,640 (GRCm39)	S243P	possibly damaging	Het
Vmn2r50	A	G	7: 9,781,905 (GRCm39)	I280T	probably damaging	Het
Zfp445	G	T	9: 122,682,211 (GRCm39)	Q577K	probably benign	Het
Zfp599	T	C	9: 22,160,957 (GRCm39)	K403E	probably damaging	Het

Other mutations in Zfp1010

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R1549:Zfp1010	UTSW	2	176,957,378 (GRCm39)	missense	probably damaging	1.00
R2125:Zfp1010	UTSW	2	176,957,195 (GRCm39)	missense	probably damaging	1.00
R3833:Zfp1010	UTSW	2	176,956,998 (GRCm39)	missense	possibly damaging	0.64
R4438:Zfp1010	UTSW	2	176,956,889 (GRCm39)	missense	possibly damaging	0.90
R4906:Zfp1010	UTSW	2	176,957,223 (GRCm39)	missense	possibly damaging	0.90
R5083:Zfp1010	UTSW	2	176,957,364 (GRCm39)	missense	probably damaging	0.99
R5802:Zfp1010	UTSW	2	176,957,049 (GRCm39)	missense	possibly damaging	0.95
R7026:Zfp1010	UTSW	2	176,957,361 (GRCm39)	missense	probably benign	0.02
R7761:Zfp1010	UTSW	2	176,957,310 (GRCm39)	missense	possibly damaging	0.66
R7993:Zfp1010	UTSW	2	176,957,015 (GRCm39)	missense	probably damaging	1.00
R8870:Zfp1010	UTSW	2	176,957,312 (GRCm39)	missense	probably benign	0.07
R8918:Zfp1010	UTSW	2	176,958,551 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ACTGCTTCCTTCAAAGGGTTTAC -3'
(R):5'- TCACAAAGCAGTCATCTCCGAATA -3'

Sequencing Primer
(F):5'- TCAAAGGGTTTACCACCTTGG -3'
(R):5'- GTGGTAAAGCCTTTGCACATAGC -3'

Posted On

2016-11-21