Mutagenetix > Incidental Mutation

Incidental Mutation 'R5771:Lypd3'

445359

Institutional Source

Beutler Lab

Gene Symbol

Lypd3

Ensembl Gene

ENSMUSG00000057454

Gene Name

Ly6/Plaur domain containing 3

Synonyms

2310061G07Rik, C4.4A

MMRRC Submission

043371-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.176) question?

Stock #

R5771 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

24636550-24641118 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 24640362 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 285 (S285P)

Ref Sequence

ENSEMBL: ENSMUSP00000079543 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080718]

AlphaFold

Q91YK8

Predicted Effect

probably benign
Transcript: ENSMUST00000080718
AA Change: S285P

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000079543
Gene: ENSMUSG00000057454
AA Change: S285P

Domain	Start	End	E-Value	Type
signal peptide	1	32	N/A	INTRINSIC
LU	33	128	7.26e-26	SMART
Pfam:UPAR_LY6	142	224	1.3e-16	PFAM
low complexity region	234	255	N/A	INTRINSIC
low complexity region	258	278	N/A	INTRINSIC
low complexity region	303	320	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000180699

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.8%

Validation Efficiency

95% (87/92)

MGI Phenotype

PHENOTYPE: Homozygous null mice show no overt epidermal phenotype and have normal squamous epithelia morphology but are lighter and leaner than controls. Males display delayed wound healing whereas females show reduced food intake and a lower incidence of invasive lesions in a BBN-induced bladder cancer model. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 82 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	T	A	11: 9,291,411	N1091K	probably damaging	Het
Adam3	C	T	8: 24,707,411	V335I	probably benign	Het
AI504432	G	A	3: 107,048,983		noncoding transcript	Het
Apcdd1	A	G	18: 62,936,956	Y98C	probably damaging	Het
Aqp9	C	T	9: 71,122,864	A214T	probably damaging	Het
Atp8b4	T	C	2: 126,378,744	D578G	probably benign	Het
Bcdin3d	T	C	15: 99,470,836	M161V	probably benign	Het
Bicral	C	T	17: 46,825,358	V309I	possibly damaging	Het
Ccdc88b	C	T	19: 6,853,835	A580T	probably benign	Het
Cdhr2	T	A	13: 54,726,695	V860E	probably damaging	Het
Clhc1	A	T	11: 29,563,854	N302Y	possibly damaging	Het
Cnnm2	T	A	19: 46,856,995		probably null	Het
Crebbp	T	C	16: 4,119,772	N709S	probably benign	Het
Ctnnal1	T	C	4: 56,826,328	S512G	probably benign	Het
Dgcr8	C	T	16: 18,272,768	V581I	probably benign	Het
Dnah7c	G	A	1: 46,639,665	V1790I	probably benign	Het
Efl1	A	G	7: 82,692,524	K452E	probably benign	Het
Epb42	A	C	2: 121,021,820	V564G	probably damaging	Het
Etv4	G	A	11: 101,771,456	P335S	probably damaging	Het
Fbxw9	T	A	8: 85,064,572		probably null	Het
Fcrls	A	T	3: 87,263,468	L3Q	probably damaging	Het
Frmd8	C	A	19: 5,869,450	A190S	probably benign	Het
Gcm2	C	T	13: 41,103,515	D253N	probably benign	Het
Gfm1	T	C	3: 67,435,562	V143A	probably benign	Het
Gigyf2	T	C	1: 87,446,328	M1226T	possibly damaging	Het
Gm10110	C	T	14: 89,897,239		noncoding transcript	Het
Gm11595	G	A	11: 99,772,555	R100C	unknown	Het
Gm13757	A	T	2: 88,446,308	V210D	possibly damaging	Het
Gm20939	T	G	17: 94,874,339	C55W	possibly damaging	Het
Gm5407	T	A	16: 49,296,866		noncoding transcript	Het
Golga4	A	G	9: 118,558,283	E1491G	probably damaging	Het
Hdac11	T	A	6: 91,169,382		probably null	Het
Hs3st3b1	C	T	11: 63,889,272	S343N	probably benign	Het
Igkv12-44	G	A	6: 69,815,091	P4S	probably benign	Het
Igkv1-35	C	T	6: 70,011,247	V22I	probably benign	Het
Kcns2	A	T	15: 34,838,922	T144S	probably benign	Het
Khdc1c	T	C	1: 21,369,042	V84A	possibly damaging	Het
Kif4-ps	T	A	12: 101,149,285		noncoding transcript	Het
Lamc2	T	G	1: 153,141,594	Y549S	probably benign	Het
Lect2	T	A	13: 56,548,341	I8F	probably benign	Het
Loxl3	T	A	6: 83,035,799		probably null	Het
Lrwd1	A	T	5: 136,123,662	I545N	possibly damaging	Het
Ltbp3	T	C	19: 5,747,544	C437R	probably damaging	Het
Mast2	T	A	4: 116,333,425	H83L	possibly damaging	Het
Mfsd4b4	A	G	10: 39,892,639	F153L	probably benign	Het
Ms4a4b	T	A	19: 11,461,242		probably null	Het
Nbr1	T	A	11: 101,559,538	L41Q	probably damaging	Het
Nlrp4a	G	A	7: 26,453,389	C628Y	probably damaging	Het
Nup160	C	T	2: 90,723,396	R1157C	probably damaging	Het
Obscn	T	A	11: 59,000,707	I7000F	unknown	Het
Olfr1287	T	A	2: 111,450,061		probably null	Het
Olfr1484	T	G	19: 13,585,508	V25G	probably damaging	Het
Olfr194	TGAAGAAGAA	TGAAGAA	16: 59,119,972		probably benign	Het
Olfr229	A	T	9: 39,910,250	Y149F	probably damaging	Het
Pdilt	T	C	7: 119,494,994	K345E	probably damaging	Het
Ppp1r12b	A	T	1: 134,773,424		probably null	Het
Ppp2r1b	A	T	9: 50,866,832	Q219L	probably damaging	Het
Prim2	A	G	1: 33,454,151	S485P	unknown	Het
Prkdc	A	G	16: 15,664,233	D380G	probably damaging	Het
Psmg1	A	G	16: 95,982,169	L243S	probably damaging	Het
Rapgef5	T	A	12: 117,721,326	V270D	probably benign	Het
Rnft1	C	T	11: 86,493,206	R311*	probably null	Het
Scaper	T	A	9: 55,816,791	I628L	probably damaging	Het
Serpina3j	C	A	12: 104,314,926	H119Q	possibly damaging	Het
Slamf6	G	A	1: 171,917,774	V23I	probably damaging	Het
Slc12a5	T	A	2: 164,973,768	M45K	possibly damaging	Het
Slc25a11	A	C	11: 70,646,190	V13G	probably damaging	Het
Slc7a14	T	A	3: 31,238,707	Y143F	probably damaging	Het
Spryd3	A	G	15: 102,116,907		probably benign	Het
Stard13	C	A	5: 151,190,011	L28F	probably damaging	Het
Susd2	C	T	10: 75,638,019	A581T	probably damaging	Het
Taf2	A	G	15: 55,059,939	V314A	probably benign	Het
Tas2r106	T	C	6: 131,678,123	N255S	possibly damaging	Het
Tbxa2r	T	A	10: 81,332,941	W155R	probably damaging	Het
Tcf3	A	G	10: 80,421,616		probably benign	Het
Ticam1	TCACACA	TCACA	17: 56,270,629		probably null	Het
Vmn2r55	T	C	7: 12,671,032	D148G	probably damaging	Het
Vmn2r77	A	G	7: 86,812,027	N854D	probably benign	Het
Vstm4	A	T	14: 32,904,569	H64L	probably benign	Het
Wdr92	A	G	11: 17,224,638	T169A	probably benign	Het
Wnk2	T	C	13: 49,102,800	T282A	probably damaging	Het
Zscan4c	A	T	7: 11,009,647	D391V	probably benign	Het

Other mutations in Lypd3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01129:Lypd3	APN	7	24640593	missense	probably benign	0.00
IGL01443:Lypd3	APN	7	24636638	missense	probably benign	0.03
R0200:Lypd3	UTSW	7	24640231	missense	probably damaging	1.00
R0726:Lypd3	UTSW	7	24638544	nonsense	probably null
R1706:Lypd3	UTSW	7	24640330	missense	probably benign	0.00
R5714:Lypd3	UTSW	7	24639069	missense	possibly damaging	0.86
R6137:Lypd3	UTSW	7	24640494	missense	probably benign	0.00
R6908:Lypd3	UTSW	7	24638433	missense	probably damaging	1.00
R6932:Lypd3	UTSW	7	24638433	missense	probably damaging	1.00
R6935:Lypd3	UTSW	7	24638433	missense	probably damaging	1.00
R7632:Lypd3	UTSW	7	24638440	missense	possibly damaging	0.60
R8769:Lypd3	UTSW	7	24638507	missense	probably damaging	0.99
R9663:Lypd3	UTSW	7	24638924	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCCAGGATTCACACTCAGCG -3'
(R):5'- AGCCTCCTTTAGCTGGGATCTG -3'

Sequencing Primer
(F):5'- GATTCACACTCAGCGGCTCTTG -3'
(R):5'- CTTTAGCTGGGATCTGGGCCC -3'

Posted On

2016-11-21