Mutagenetix > Incidental Mutation

Incidental Mutation 'R5745:Vmn2r45'

445747

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r45

Ensembl Gene

ENSMUSG00000090662

Gene Name

vomeronasal 2, receptor 45

Synonyms

MMRRC Submission

043198-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.065) question?

Stock #

R5745 (G1)

Quality Score

126

Status

Not validated

Chromosome

Chromosomal Location

8474468-8491958 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 8486074 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 405 (I405L)

Ref Sequence

ENSEMBL: ENSMUSP00000129466 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164845]

AlphaFold

L7N2B5

Predicted Effect

probably benign
Transcript: ENSMUST00000164845
AA Change: I405L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000129466
Gene: ENSMUSG00000090662
AA Change: I405L

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	154	469	4.5e-26	PFAM
Pfam:NCD3G	512	565	6.4e-21	PFAM
Pfam:7tm_3	598	833	2.1e-54	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110082I17Rik	G	A	5: 139,349,828 (GRCm39)	R74W	probably damaging	Het
4933407L21Rik	T	G	1: 85,858,995 (GRCm39)		probably null	Het
Adcy8	A	T	15: 64,792,320 (GRCm39)	I212N	possibly damaging	Het
Cobll1	T	C	2: 64,928,801 (GRCm39)	T879A	probably damaging	Het
Copb2	T	C	9: 98,456,164 (GRCm39)	S233P	probably damaging	Het
Cpa5	T	A	6: 30,630,436 (GRCm39)	M330K	probably damaging	Het
Dgcr8	A	T	16: 18,098,307 (GRCm39)	N361K	probably benign	Het
Dmxl1	A	G	18: 49,979,653 (GRCm39)	E96G	probably benign	Het
Dock8	T	A	19: 25,107,761 (GRCm39)	N830K	probably benign	Het
Ephb1	C	T	9: 102,072,633 (GRCm39)	D49N	probably benign	Het
Fer1l6	A	G	15: 58,443,238 (GRCm39)	I514V	probably benign	Het
Fpr1	A	G	17: 18,097,344 (GRCm39)	I215T	probably benign	Het
Hectd4	G	A	5: 121,491,565 (GRCm39)	V3668M	possibly damaging	Het
Ighv3-4	T	A	12: 114,217,388 (GRCm39)	I68L	probably benign	Het
Intu	A	G	3: 40,647,402 (GRCm39)		probably null	Het
Kel	C	T	6: 41,675,961 (GRCm39)	G243E	probably damaging	Het
Mycbp2	A	C	14: 103,393,889 (GRCm39)	S2781A	possibly damaging	Het
Myom2	T	A	8: 15,172,705 (GRCm39)	S1211T	probably benign	Het
Nrp1	A	T	8: 129,194,929 (GRCm39)	I462F	probably benign	Het
Or10d3	T	C	9: 39,461,987 (GRCm39)	Y60C	probably damaging	Het
Or6c5c	T	A	10: 129,299,307 (GRCm39)	I254N	probably damaging	Het
Pcsk1	A	C	13: 75,280,079 (GRCm39)	S635R	probably benign	Het
Pms1	A	T	1: 53,246,861 (GRCm39)	Y280*	probably null	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,127 (GRCm39)		probably benign	Het
Sema3b	C	A	9: 107,478,628 (GRCm39)	A356S	probably damaging	Het
Shoc2	C	A	19: 54,018,323 (GRCm39)	T485K	probably benign	Het
Slc7a7	G	A	14: 54,615,292 (GRCm39)	S235L	possibly damaging	Het
Smcr8	A	T	11: 60,674,977 (GRCm39)	T918S	probably benign	Het
Tafa1	G	A	6: 96,626,146 (GRCm39)	R128Q	probably damaging	Het
Tle3	C	A	9: 61,322,133 (GRCm39)	F719L	probably damaging	Het
Vmn2r57	A	T	7: 41,097,895 (GRCm39)	H57Q	possibly damaging	Het

Other mutations in Vmn2r45

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01287:Vmn2r45	APN	7	8,488,622 (GRCm39)	missense	probably benign	0.00
IGL01322:Vmn2r45	APN	7	8,484,332 (GRCm39)	missense	possibly damaging	0.49
IGL01402:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01404:Vmn2r45	APN	7	8,484,467 (GRCm39)	missense	probably damaging	0.98
IGL01529:Vmn2r45	APN	7	8,486,493 (GRCm39)	missense	probably benign
IGL01596:Vmn2r45	APN	7	8,486,272 (GRCm39)	missense	probably damaging	0.98
IGL01646:Vmn2r45	APN	7	8,486,337 (GRCm39)	missense	probably benign	0.18
IGL01819:Vmn2r45	APN	7	8,488,556 (GRCm39)	missense	probably benign	0.00
IGL02096:Vmn2r45	APN	7	8,484,443 (GRCm39)	missense	probably damaging	0.99
IGL02130:Vmn2r45	APN	7	8,486,556 (GRCm39)	missense	possibly damaging	0.46
IGL02409:Vmn2r45	APN	7	8,488,727 (GRCm39)	missense	probably benign	0.03
IGL02517:Vmn2r45	APN	7	8,486,185 (GRCm39)	nonsense	probably null
IGL02633:Vmn2r45	APN	7	8,488,728 (GRCm39)	missense	probably benign	0.01
IGL02940:Vmn2r45	APN	7	8,475,369 (GRCm39)	missense	probably damaging	0.99
IGL03281:Vmn2r45	APN	7	8,486,603 (GRCm39)	missense	probably damaging	1.00
IGL03358:Vmn2r45	APN	7	8,474,715 (GRCm39)	missense	probably damaging	1.00
BB004:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
BB014:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R0382:Vmn2r45	UTSW	7	8,486,098 (GRCm39)	missense	probably benign	0.06
R0532:Vmn2r45	UTSW	7	8,474,820 (GRCm39)	missense	probably damaging	0.99
R0932:Vmn2r45	UTSW	7	8,478,380 (GRCm39)	missense	probably damaging	1.00
R1125:Vmn2r45	UTSW	7	8,488,542 (GRCm39)	missense	probably benign	0.00
R1580:Vmn2r45	UTSW	7	8,474,746 (GRCm39)	missense	possibly damaging	0.95
R1817:Vmn2r45	UTSW	7	8,475,372 (GRCm39)	missense	probably damaging	1.00
R1996:Vmn2r45	UTSW	7	8,475,024 (GRCm39)	missense	probably damaging	1.00
R2050:Vmn2r45	UTSW	7	8,475,021 (GRCm39)	missense	probably damaging	1.00
R2284:Vmn2r45	UTSW	7	8,488,765 (GRCm39)	missense	probably benign	0.02
R4019:Vmn2r45	UTSW	7	8,474,580 (GRCm39)	nonsense	probably null
R4227:Vmn2r45	UTSW	7	8,486,277 (GRCm39)	missense	probably damaging	0.98
R4381:Vmn2r45	UTSW	7	8,474,912 (GRCm39)	nonsense	probably null
R4618:Vmn2r45	UTSW	7	8,486,436 (GRCm39)	missense	probably benign	0.00
R4624:Vmn2r45	UTSW	7	8,484,341 (GRCm39)	missense	probably damaging	1.00
R4704:Vmn2r45	UTSW	7	8,486,535 (GRCm39)	nonsense	probably null
R4735:Vmn2r45	UTSW	7	8,486,472 (GRCm39)	missense	probably damaging	1.00
R4868:Vmn2r45	UTSW	7	8,484,480 (GRCm39)	missense	probably benign	0.06
R4983:Vmn2r45	UTSW	7	8,486,116 (GRCm39)	missense	probably damaging	0.99
R5246:Vmn2r45	UTSW	7	8,486,251 (GRCm39)	missense	probably benign	0.00
R5430:Vmn2r45	UTSW	7	8,486,333 (GRCm39)	nonsense	probably null
R5504:Vmn2r45	UTSW	7	8,486,176 (GRCm39)	missense	probably benign	0.03
R5511:Vmn2r45	UTSW	7	8,474,832 (GRCm39)	missense	probably benign	0.19
R5814:Vmn2r45	UTSW	7	8,474,475 (GRCm39)	missense	probably benign	0.00
R6223:Vmn2r45	UTSW	7	8,486,301 (GRCm39)	missense	probably benign
R6267:Vmn2r45	UTSW	7	8,475,207 (GRCm39)	missense	probably benign	0.26
R6623:Vmn2r45	UTSW	7	8,474,500 (GRCm39)	missense	probably benign	0.09
R6999:Vmn2r45	UTSW	7	8,486,219 (GRCm39)	missense	probably benign
R7242:Vmn2r45	UTSW	7	8,488,612 (GRCm39)	nonsense	probably null
R7491:Vmn2r45	UTSW	7	8,484,342 (GRCm39)	missense	probably benign	0.27
R7620:Vmn2r45	UTSW	7	8,486,222 (GRCm39)	nonsense	probably null
R7719:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7720:Vmn2r45	UTSW	7	8,486,460 (GRCm39)	missense	probably damaging	0.99
R7853:Vmn2r45	UTSW	7	8,485,987 (GRCm39)	missense	possibly damaging	0.90
R7873:Vmn2r45	UTSW	7	8,486,074 (GRCm39)	missense	probably benign	0.00
R7882:Vmn2r45	UTSW	7	8,486,409 (GRCm39)	missense	possibly damaging	0.82
R7927:Vmn2r45	UTSW	7	8,486,513 (GRCm39)	missense	probably benign
R8684:Vmn2r45	UTSW	7	8,486,511 (GRCm39)	missense	probably damaging	0.99
R8780:Vmn2r45	UTSW	7	8,484,371 (GRCm39)	missense	possibly damaging	0.94
R8811:Vmn2r45	UTSW	7	8,474,881 (GRCm39)	missense	probably damaging	1.00
R8893:Vmn2r45	UTSW	7	8,488,619 (GRCm39)	missense	probably damaging	1.00
R9043:Vmn2r45	UTSW	7	8,474,904 (GRCm39)	missense	possibly damaging	0.92
R9208:Vmn2r45	UTSW	7	8,486,298 (GRCm39)	missense	probably damaging	1.00
R9327:Vmn2r45	UTSW	7	8,474,532 (GRCm39)	missense	probably damaging	1.00
R9586:Vmn2r45	UTSW	7	8,486,050 (GRCm39)	missense	probably benign	0.01
R9599:Vmn2r45	UTSW	7	8,478,457 (GRCm39)	critical splice acceptor site	probably null
U24488:Vmn2r45	UTSW	7	8,475,361 (GRCm39)	missense	probably damaging	1.00
Z1088:Vmn2r45	UTSW	7	8,474,484 (GRCm39)	missense	probably benign	0.16

Predicted Primers

PCR Primer
(F):5'- TTGTGTGCTCTGTATAAAACCACAC -3'
(R):5'- TGGTTCCATCTCAGAAACACAG -3'

Sequencing Primer
(F):5'- ACCACACATTTTCATTGCTAATCTG -3'
(R):5'- TGAAGACTCAGCATCTACC -3'

Posted On

2016-11-21