Mutagenetix > Incidental Mutation

Incidental Mutation 'R5745:Vmn2r57'

445748

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r57

Ensembl Gene

ENSMUSG00000066537

Gene Name

vomeronasal 2, receptor 57

Synonyms

EG269902

MMRRC Submission

043198-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.113) question?

Stock #

R5745 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

41399732-41448641 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 41448471 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Histidine to Glutamine at position 57 (H57Q)

Ref Sequence

ENSEMBL: ENSMUSP00000125817 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094532] [ENSMUST00000165029]

AlphaFold

L7N269

Predicted Effect

probably benign
Transcript: ENSMUST00000094532

Predicted Effect

possibly damaging
Transcript: ENSMUST00000165029
AA Change: H57Q

PolyPhen 2 Score 0.508 (Sensitivity: 0.88; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000125817
Gene: ENSMUSG00000066537
AA Change: H57Q

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	471	1.4e-44	PFAM
Pfam:NCD3G	514	567	2.7e-23	PFAM
Pfam:7tm_3	600	835	1.8e-52	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 31 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
3110082I17Rik	G	A	5: 139,364,073 (GRCm38)	R74W	probably damaging	Het
4933407L21Rik	T	G	1: 85,931,274 (GRCm38)		probably null	Het
Adcy8	A	T	15: 64,920,471 (GRCm38)	I212N	possibly damaging	Het
Cobll1	T	C	2: 65,098,457 (GRCm38)	T879A	probably damaging	Het
Copb2	T	C	9: 98,574,111 (GRCm38)	S233P	probably damaging	Het
Cpa5	T	A	6: 30,630,437 (GRCm38)	M330K	probably damaging	Het
Dgcr8	A	T	16: 18,280,443 (GRCm38)	N361K	probably benign	Het
Dmxl1	A	G	18: 49,846,586 (GRCm38)	E96G	probably benign	Het
Dock8	T	A	19: 25,130,397 (GRCm38)	N830K	probably benign	Het
Ephb1	C	T	9: 102,195,434 (GRCm38)	D49N	probably benign	Het
Fer1l6	A	G	15: 58,571,389 (GRCm38)	I514V	probably benign	Het
Fpr1	A	G	17: 17,877,082 (GRCm38)	I215T	probably benign	Het
Hectd4	G	A	5: 121,353,502 (GRCm38)	V3668M	possibly damaging	Het
Ighv3-4	T	A	12: 114,253,768 (GRCm38)	I68L	probably benign	Het
Intu	A	G	3: 40,692,972 (GRCm38)		probably null	Het
Kel	C	T	6: 41,699,027 (GRCm38)	G243E	probably damaging	Het
Mycbp2	A	C	14: 103,156,453 (GRCm38)	S2781A	possibly damaging	Het
Myom2	T	A	8: 15,122,705 (GRCm38)	S1211T	probably benign	Het
Nrp1	A	T	8: 128,468,448 (GRCm38)	I462F	probably benign	Het
Or10d3	T	C	9: 39,550,691 (GRCm38)	Y60C	probably damaging	Het
Or6c5c	T	A	10: 129,463,438 (GRCm38)	I254N	probably damaging	Het
Pcsk1	A	C	13: 75,131,960 (GRCm38)	S635R	probably benign	Het
Pms1	A	T	1: 53,207,702 (GRCm38)	Y280*	probably null	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,579,920 (GRCm38)		probably benign	Het
Sema3b	C	A	9: 107,601,429 (GRCm38)	A356S	probably damaging	Het
Shoc2	C	A	19: 54,029,892 (GRCm38)	T485K	probably benign	Het
Slc7a7	G	A	14: 54,377,835 (GRCm38)	S235L	possibly damaging	Het
Smcr8	A	T	11: 60,784,151 (GRCm38)	T918S	probably benign	Het
Tafa1	G	A	6: 96,649,185 (GRCm38)	R128Q	probably damaging	Het
Tle3	C	A	9: 61,414,851 (GRCm38)	F719L	probably damaging	Het
Vmn2r45	T	A	7: 8,483,075 (GRCm38)	I405L	probably benign	Het

Other mutations in Vmn2r57

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00159:Vmn2r57	APN	7	41,428,785 (GRCm38)	missense	probably benign
IGL01108:Vmn2r57	APN	7	41,427,584 (GRCm38)	missense	probably benign	0.01
IGL01112:Vmn2r57	APN	7	41,425,043 (GRCm38)	missense	probably damaging	1.00
IGL01516:Vmn2r57	APN	7	41,399,946 (GRCm38)	missense	probably damaging	1.00
IGL01880:Vmn2r57	APN	7	41,400,195 (GRCm38)	missense	possibly damaging	0.73
IGL02117:Vmn2r57	APN	7	41,400,450 (GRCm38)	missense	probably benign	0.00
IGL02500:Vmn2r57	APN	7	41,428,226 (GRCm38)	missense	probably benign
IGL02801:Vmn2r57	APN	7	41,448,632 (GRCm38)	missense	probably benign	0.13
IGL02993:Vmn2r57	APN	7	41,428,074 (GRCm38)	missense	probably benign	0.04
IGL02996:Vmn2r57	APN	7	41,399,741 (GRCm38)	missense	probably benign	0.02
R0008:Vmn2r57	UTSW	7	41,400,652 (GRCm38)	missense	probably damaging	1.00
R0032:Vmn2r57	UTSW	7	41,399,733 (GRCm38)	splice site	probably null
R0305:Vmn2r57	UTSW	7	41,427,543 (GRCm38)	missense	probably benign	0.00
R0469:Vmn2r57	UTSW	7	41,427,792 (GRCm38)	missense	possibly damaging	0.58
R0510:Vmn2r57	UTSW	7	41,427,792 (GRCm38)	missense	possibly damaging	0.58
R0847:Vmn2r57	UTSW	7	41,428,801 (GRCm38)	missense	probably benign	0.00
R1025:Vmn2r57	UTSW	7	41,427,804 (GRCm38)	missense	probably benign	0.24
R1081:Vmn2r57	UTSW	7	41,428,211 (GRCm38)	missense	possibly damaging	0.47
R1479:Vmn2r57	UTSW	7	41,427,830 (GRCm38)	missense	possibly damaging	0.45
R1579:Vmn2r57	UTSW	7	41,400,124 (GRCm38)	missense	probably benign	0.38
R1764:Vmn2r57	UTSW	7	41,400,643 (GRCm38)	missense	probably damaging	1.00
R1848:Vmn2r57	UTSW	7	41,428,107 (GRCm38)	missense	probably damaging	1.00
R2006:Vmn2r57	UTSW	7	41,448,577 (GRCm38)	missense	probably benign	0.00
R2197:Vmn2r57	UTSW	7	41,428,825 (GRCm38)	critical splice acceptor site	probably null
R2242:Vmn2r57	UTSW	7	41,428,074 (GRCm38)	missense	probably benign	0.00
R2394:Vmn2r57	UTSW	7	41,400,195 (GRCm38)	missense	possibly damaging	0.73
R3937:Vmn2r57	UTSW	7	41,428,130 (GRCm38)	missense	probably damaging	0.97
R4193:Vmn2r57	UTSW	7	41,428,239 (GRCm38)	missense	probably benign
R4423:Vmn2r57	UTSW	7	41,426,640 (GRCm38)	missense	probably damaging	1.00
R4865:Vmn2r57	UTSW	7	41,400,468 (GRCm38)	missense	probably damaging	1.00
R4947:Vmn2r57	UTSW	7	41,400,495 (GRCm38)	missense	probably damaging	1.00
R5042:Vmn2r57	UTSW	7	41,428,662 (GRCm38)	missense	probably benign	0.06
R5084:Vmn2r57	UTSW	7	41,426,550 (GRCm38)	critical splice donor site	probably null
R5177:Vmn2r57	UTSW	7	41,400,240 (GRCm38)	missense	probably benign	0.31
R5192:Vmn2r57	UTSW	7	41,427,939 (GRCm38)	missense	probably damaging	0.96
R5289:Vmn2r57	UTSW	7	41,399,974 (GRCm38)	missense	probably damaging	0.99
R6051:Vmn2r57	UTSW	7	41,448,472 (GRCm38)	missense	probably benign	0.00
R6155:Vmn2r57	UTSW	7	41,428,690 (GRCm38)	missense	probably benign	0.14
R6248:Vmn2r57	UTSW	7	41,399,860 (GRCm38)	missense	probably benign
R6381:Vmn2r57	UTSW	7	41,428,818 (GRCm38)	missense	probably benign	0.08
R7019:Vmn2r57	UTSW	7	41,428,665 (GRCm38)	missense	probably damaging	1.00
R7126:Vmn2r57	UTSW	7	41,399,794 (GRCm38)	missense	possibly damaging	0.93
R7146:Vmn2r57	UTSW	7	41,448,471 (GRCm38)	missense	possibly damaging	0.51
R7215:Vmn2r57	UTSW	7	41,400,286 (GRCm38)	missense	probably benign	0.00
R7432:Vmn2r57	UTSW	7	41,426,724 (GRCm38)	missense	probably benign	0.01
R7633:Vmn2r57	UTSW	7	41,425,089 (GRCm38)	missense	possibly damaging	0.76
R7811:Vmn2r57	UTSW	7	41,425,015 (GRCm38)	nonsense	probably null
R8025:Vmn2r57	UTSW	7	41,426,759 (GRCm38)	missense	probably benign	0.00
R8332:Vmn2r57	UTSW	7	41,400,253 (GRCm38)	missense	probably benign	0.01
R8345:Vmn2r57	UTSW	7	41,427,544 (GRCm38)	missense	possibly damaging	0.81
R8360:Vmn2r57	UTSW	7	41,400,216 (GRCm38)	missense	probably damaging	1.00
R8738:Vmn2r57	UTSW	7	41,427,596 (GRCm38)	missense	probably benign	0.00
R8758:Vmn2r57	UTSW	7	41,428,739 (GRCm38)	missense	probably damaging	1.00
R8955:Vmn2r57	UTSW	7	41,400,147 (GRCm38)	missense	possibly damaging	0.64
R8985:Vmn2r57	UTSW	7	41,399,835 (GRCm38)	missense	probably benign
R9108:Vmn2r57	UTSW	7	41,428,768 (GRCm38)	missense	possibly damaging	0.87
R9160:Vmn2r57	UTSW	7	41,426,735 (GRCm38)	missense	possibly damaging	0.48
R9354:Vmn2r57	UTSW	7	41,400,239 (GRCm38)	missense	probably benign	0.01
R9566:Vmn2r57	UTSW	7	41,427,665 (GRCm38)	missense	probably benign	0.32
R9633:Vmn2r57	UTSW	7	41,426,582 (GRCm38)	missense	probably benign	0.00
X0026:Vmn2r57	UTSW	7	41,428,561 (GRCm38)	missense	possibly damaging	0.91
X0026:Vmn2r57	UTSW	7	41,428,125 (GRCm38)	missense	probably benign	0.03
X0065:Vmn2r57	UTSW	7	41,427,971 (GRCm38)	missense	probably benign	0.09
Z1176:Vmn2r57	UTSW	7	41,400,498 (GRCm38)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- GCTCAGAAGTACCAGATCATACTTAG -3'
(R):5'- ATCCATCAGTCTTATGGATGGGC -3'

Sequencing Primer
(F):5'- TCAATTCATCTGGCATTACAAGC -3'
(R):5'- CCATCAGTCTTATGGATGGGCATATG -3'

Posted On

2016-11-21