Mutagenetix > Incidental Mutation

Incidental Mutation 'R5746:Zfp146'

445784

Institutional Source

Beutler Lab

Gene Symbol

Zfp146

Ensembl Gene

ENSMUSG00000037029

Gene Name

zinc finger protein 146

Synonyms

OZF

MMRRC Submission

043199-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.190) question?

Stock #

R5746 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

29860694-29869175 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 29861818 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 75 (S75G)

Ref Sequence

ENSEMBL: ENSMUSP00000058588 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000062181]

AlphaFold

Q8BQN6

Predicted Effect

probably benign
Transcript: ENSMUST00000062181
AA Change: S75G

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000058588
Gene: ENSMUSG00000037029
AA Change: S75G

Domain	Start	End	E-Value	Type
ZnF_C2H2	16	38	1.01e-1	SMART
ZnF_C2H2	44	66	5.5e-3	SMART
ZnF_C2H2	72	94	3.44e-4	SMART
ZnF_C2H2	100	122	2.27e-4	SMART
ZnF_C2H2	128	150	1.98e-4	SMART
ZnF_C2H2	156	178	3.89e-3	SMART
ZnF_C2H2	184	206	6.99e-5	SMART
ZnF_C2H2	212	234	1.6e-4	SMART
ZnF_C2H2	240	262	5.5e-3	SMART
ZnF_C2H2	268	290	1.82e-3	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000136508

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 28 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adora3	A	G	3: 105,815,126 (GRCm39)	E292G	possibly damaging	Het
Ank1	C	T	8: 23,606,612 (GRCm39)	T1187I	probably damaging	Het
Ankrd16	T	A	2: 11,789,178 (GRCm39)	I255K	probably damaging	Het
Atp6ap1l	T	C	13: 91,031,698 (GRCm39)	N328S	probably benign	Het
Cbln3	A	G	14: 56,120,601 (GRCm39)	S144P	probably damaging	Het
Cdh12	T	A	15: 21,358,810 (GRCm39)	L78Q	probably null	Het
Cpsf1	C	T	15: 76,484,037 (GRCm39)	R761H	probably benign	Het
Cyp2d10	T	G	15: 82,289,472 (GRCm39)	E213D	probably benign	Het
Gba2	T	C	4: 43,568,465 (GRCm39)		probably null	Het
Glb1l2	G	A	9: 26,708,086 (GRCm39)	A29V	probably benign	Het
Gm11149	C	T	9: 49,457,494 (GRCm39)	G393S	probably damaging	Het
Igkv6-29	C	A	6: 70,115,584 (GRCm39)	G70V	possibly damaging	Het
Kcnma1	T	C	14: 23,544,635 (GRCm39)	N574S	probably damaging	Het
Kdm2b	A	G	5: 123,017,427 (GRCm39)	V1099A	probably damaging	Het
Kel	C	T	6: 41,675,961 (GRCm39)	G243E	probably damaging	Het
Ly6g	A	T	15: 75,028,596 (GRCm39)	N19Y	possibly damaging	Het
Nexn	TCTTCCTTC	TCTTC	3: 151,948,513 (GRCm39)		probably benign	Het
Nod2	T	C	8: 89,390,970 (GRCm39)	S411P	probably damaging	Het
Plekha6	G	C	1: 133,200,045 (GRCm39)	R208P	possibly damaging	Het
Ptpn14	G	A	1: 189,578,610 (GRCm39)		probably null	Het
Smg6	G	A	11: 75,030,113 (GRCm39)	G211E	probably damaging	Het
Stx16	G	A	2: 173,935,292 (GRCm39)	G156R	probably damaging	Het
Tbc1d15	T	C	10: 115,046,184 (GRCm39)	S440G	probably damaging	Het
Tpm2	A	G	4: 43,519,731 (GRCm39)	V85A	possibly damaging	Het
Ttf1	G	T	2: 28,955,754 (GRCm39)	A373S	probably damaging	Het
Vmn2r11	T	A	5: 109,201,560 (GRCm39)	T315S	probably benign	Het
Zcchc14	T	C	8: 122,331,378 (GRCm39)		probably benign	Het
Zfp994	A	G	17: 22,420,254 (GRCm39)	Y232H	probably damaging	Het

Other mutations in Zfp146

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02170:Zfp146	APN	7	29,861,905 (GRCm39)	missense	possibly damaging	0.51
IGL02485:Zfp146	APN	7	29,862,015 (GRCm39)	missense	probably benign
IGL02796:Zfp146	UTSW	7	29,861,983 (GRCm39)	missense	probably benign	0.01
R2043:Zfp146	UTSW	7	29,861,664 (GRCm39)	missense	possibly damaging	0.92
R4166:Zfp146	UTSW	7	29,861,865 (GRCm39)	missense	possibly damaging	0.50
R4385:Zfp146	UTSW	7	29,861,847 (GRCm39)	missense	probably benign
R5270:Zfp146	UTSW	7	29,861,900 (GRCm39)	missense	probably benign	0.03
R5271:Zfp146	UTSW	7	29,861,900 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- CATGCACAATCAGCGACGTG -3'
(R):5'- AAGCCCCTTTGCCTGTAAGG -3'

Sequencing Primer
(F):5'- CAGGTACTTTTTCTGGCCAAAAGC -3'
(R):5'- GTATGTGGGAAACTCTTCAGCCAC -3'

Posted On

2016-11-21