Mutagenetix > Incidental Mutation

Incidental Mutation 'R5791:Zfp14'

446973

Institutional Source

Beutler Lab

Gene Symbol

Zfp14

Ensembl Gene

ENSMUSG00000053985

Gene Name

zinc finger protein 14

Synonyms

4732429I09Rik, Zfp-14, Krox-9

MMRRC Submission

043207-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5791 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

29735784-29750805 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 29737687 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Proline at position 433 (S433P)

Ref Sequence

ENSEMBL: ENSMUSP00000146824 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077787] [ENSMUST00000207072] [ENSMUST00000207873]

AlphaFold

P10755

Predicted Effect

probably damaging
Transcript: ENSMUST00000077787
AA Change: S433P

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000076960
Gene: ENSMUSG00000053985
AA Change: S433P

Domain	Start	End	E-Value	Type
KRAB	8	69	2.39e-21	SMART
ZnF_C2H2	140	162	8.47e-4	SMART
ZnF_C2H2	168	190	5.9e-3	SMART
ZnF_C2H2	196	218	2.75e-3	SMART
ZnF_C2H2	224	246	6.42e-4	SMART
ZnF_C2H2	252	274	1.03e-2	SMART
ZnF_C2H2	280	302	5.5e-3	SMART
ZnF_C2H2	308	330	2.02e-1	SMART
ZnF_C2H2	336	358	8.6e-5	SMART
ZnF_C2H2	364	386	4.17e-3	SMART
ZnF_C2H2	392	414	2.57e-3	SMART
ZnF_C2H2	420	442	9.44e-2	SMART
ZnF_C2H2	448	470	1.03e-2	SMART
ZnF_C2H2	476	498	5.9e-3	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000207072
AA Change: S433P

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

Predicted Effect

probably damaging
Transcript: ENSMUST00000207873
AA Change: S433P

PolyPhen 2 Score 0.992 (Sensitivity: 0.70; Specificity: 0.97)

Coding Region Coverage

1x: 99.3%
3x: 98.8%
10x: 97.6%
20x: 96.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam25	G	C	8: 41,207,257 (GRCm39)	Q174H	probably benign	Het
Adipor2	A	T	6: 119,338,866 (GRCm39)	M129K	possibly damaging	Het
Arhgap29	T	A	3: 121,807,894 (GRCm39)	M616K	probably damaging	Het
Calcrl	A	T	2: 84,181,609 (GRCm39)	F180I	probably damaging	Het
Cdh4	T	C	2: 179,537,560 (GRCm39)	V864A	probably damaging	Het
Cep78	A	G	19: 15,938,436 (GRCm39)	F504S	probably benign	Het
Coasy	A	G	11: 100,975,211 (GRCm39)		probably null	Het
Dnah3	T	C	7: 119,530,696 (GRCm39)	N751S	probably benign	Het
Ecpas	C	T	4: 58,814,027 (GRCm39)	E1360K	possibly damaging	Het
Ecpas	T	A	4: 58,822,111 (GRCm39)	D1152V	probably damaging	Het
Eea1	T	A	10: 95,855,857 (GRCm39)	N631K	probably benign	Het
Fam149b	A	G	14: 20,401,394 (GRCm39)	K27R	probably damaging	Het
Fbxw26	A	G	9: 109,574,221 (GRCm39)	W42R	probably damaging	Het
Gas6	A	G	8: 13,520,217 (GRCm39)		probably null	Het
Gfral	C	T	9: 76,104,328 (GRCm39)	R228Q	probably benign	Het
Gm11595	G	A	11: 99,663,381 (GRCm39)	R100C	unknown	Het
Gmppa	T	C	1: 75,418,899 (GRCm39)	V324A	possibly damaging	Het
Gstm2	C	T	3: 107,891,444 (GRCm39)		probably null	Het
Kcng3	A	G	17: 83,895,639 (GRCm39)	S276P	probably benign	Het
Lrrd1	T	C	5: 3,901,254 (GRCm39)	S520P	probably benign	Het
Lrrn2	A	G	1: 132,865,505 (GRCm39)	N190S	probably benign	Het
Lrwd1	G	C	5: 136,159,887 (GRCm39)	A392G	probably benign	Het
Mab21l2	A	G	3: 86,454,044 (GRCm39)	Y319H	probably damaging	Het
Ndufv3	A	G	17: 31,746,382 (GRCm39)	N91D	probably benign	Het
Nfatc2	T	A	2: 168,378,313 (GRCm39)	M451L	probably benign	Het
Or10g9b	A	T	9: 39,918,030 (GRCm39)	S72T	probably damaging	Het
Pcdhgb2	G	T	18: 37,825,393 (GRCm39)	V795F	possibly damaging	Het
Pdcd11	T	A	19: 47,099,430 (GRCm39)	M843K	possibly damaging	Het
Pla2g4c	T	A	7: 13,073,617 (GRCm39)	N221K	probably benign	Het
Ppox	T	C	1: 171,104,885 (GRCm39)	Y422C	probably damaging	Het
Retreg3	A	G	11: 100,991,769 (GRCm39)	S55P	probably damaging	Het
Rnf103	C	A	6: 71,485,909 (GRCm39)	T180K	probably damaging	Het
Tbl3	A	T	17: 24,923,408 (GRCm39)	L307H	probably damaging	Het
Tex26	A	T	5: 149,363,240 (GRCm39)		probably null	Het
Tln2	A	T	9: 67,293,887 (GRCm39)	I247K	probably damaging	Het
Txlnb	T	C	10: 17,674,876 (GRCm39)	S10P	probably benign	Het
Vwde	C	A	6: 13,195,985 (GRCm39)	E347*	probably null	Het
Wasf1	G	A	10: 40,802,570 (GRCm39)	R75Q	probably damaging	Het
Zfp647	G	T	15: 76,802,206 (GRCm39)	A2E	unknown	Het

Other mutations in Zfp14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00790:Zfp14	APN	7	29,738,312 (GRCm39)	nonsense	probably null
IGL01018:Zfp14	APN	7	29,737,526 (GRCm39)	missense	probably damaging	1.00
IGL01060:Zfp14	APN	7	29,737,510 (GRCm39)	missense	probably damaging	1.00
IGL03223:Zfp14	APN	7	29,737,858 (GRCm39)	missense	probably damaging	1.00
R0052:Zfp14	UTSW	7	29,737,753 (GRCm39)	missense	probably damaging	0.99
R0052:Zfp14	UTSW	7	29,737,753 (GRCm39)	missense	probably damaging	0.99
R1860:Zfp14	UTSW	7	29,738,116 (GRCm39)	missense	probably damaging	1.00
R2852:Zfp14	UTSW	7	29,738,596 (GRCm39)	missense	probably benign	0.01
R2887:Zfp14	UTSW	7	29,738,190 (GRCm39)	missense	probably damaging	0.98
R4585:Zfp14	UTSW	7	29,738,341 (GRCm39)	missense	probably damaging	0.99
R4586:Zfp14	UTSW	7	29,738,341 (GRCm39)	missense	probably damaging	0.99
R4625:Zfp14	UTSW	7	29,738,020 (GRCm39)	nonsense	probably null
R4988:Zfp14	UTSW	7	29,737,482 (GRCm39)	missense	probably benign
R6709:Zfp14	UTSW	7	29,737,557 (GRCm39)	missense	probably damaging	1.00
R7799:Zfp14	UTSW	7	29,738,368 (GRCm39)	missense	possibly damaging	0.87
R8375:Zfp14	UTSW	7	29,738,579 (GRCm39)	missense	possibly damaging	0.87
R8784:Zfp14	UTSW	7	29,742,961 (GRCm39)	missense	probably damaging	1.00
R8997:Zfp14	UTSW	7	29,737,600 (GRCm39)	missense	probably damaging	1.00
R9721:Zfp14	UTSW	7	29,738,609 (GRCm39)	missense	probably benign	0.00
X0017:Zfp14	UTSW	7	29,738,082 (GRCm39)	missense	probably damaging	0.98
Z1186:Zfp14	UTSW	7	29,738,577 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- GAGCTTGTCAGGTTCCACTGTG -3'
(R):5'- ACCTTCAGTAGCTACTCCCAG -3'

Sequencing Primer
(F):5'- CCACTGTGGATCTTCTGGTGC -3'
(R):5'- GCTACTCCCAGCTGATTTCAC -3'

Posted On

2016-12-15