Mutagenetix > Incidental Mutation

Incidental Mutation 'R5785:Vmn2r3'

447951

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r3

Ensembl Gene

ENSMUSG00000091572

Gene Name

vomeronasal 2, receptor 3

Synonyms

EG637004

MMRRC Submission

043206-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5785 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

64166225-64197130 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 64166444 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 896 (T896S)

Ref Sequence

ENSEMBL: ENSMUSP00000134891 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170244] [ENSMUST00000176328]

AlphaFold

H3BJ88

Predicted Effect

possibly damaging
Transcript: ENSMUST00000170244
AA Change: T868S

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000126165
Gene: ENSMUSG00000091572
AA Change: T868S

Domain	Start	End	E-Value	Type
Pfam:ANF_receptor	64	479	4e-64	PFAM
Pfam:NCD3G	521	574	1.1e-17	PFAM
Pfam:7tm_3	605	842	2.9e-75	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000176328
AA Change: T896S

PolyPhen 2 Score 0.895 (Sensitivity: 0.82; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000134891
Gene: ENSMUSG00000091572
AA Change: T896S

Domain	Start	End	E-Value	Type
signal peptide	1	22	N/A	INTRINSIC
Pfam:ANF_receptor	92	507	9.5e-66	PFAM
Pfam:NCD3G	549	602	8.8e-17	PFAM
Pfam:7tm_3	635	869	8.5e-48	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.4%
20x: 95.6%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 38 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A4gnt	A	T	9: 99,502,725 (GRCm39)	N295I	probably damaging	Het
Abcg3	G	A	5: 105,116,036 (GRCm39)	A266V	probably damaging	Het
Apbb1	C	A	7: 105,216,922 (GRCm39)	D254Y	probably damaging	Het
Arhgap39	T	C	15: 76,621,618 (GRCm39)	M328V	probably benign	Het
Bub1b	T	A	2: 118,440,325 (GRCm39)	V143D	probably damaging	Het
Celsr3	A	G	9: 108,704,996 (GRCm39)	D493G	probably damaging	Het
Cers4	T	A	8: 4,566,992 (GRCm39)		probably null	Het
Clec4d	A	G	6: 123,251,729 (GRCm39)	R204G	probably benign	Het
Cngb1	C	A	8: 95,980,823 (GRCm39)	R910L	possibly damaging	Het
Coro2a	A	T	4: 46,564,691 (GRCm39)	N18K	probably benign	Het
Cwf19l1	A	G	19: 44,110,380 (GRCm39)	F290S	probably damaging	Het
Esp18	C	T	17: 39,720,839 (GRCm39)	T28I	probably damaging	Het
Hdac10	A	T	15: 89,011,148 (GRCm39)	F205Y	probably benign	Het
Hhip	T	A	8: 80,724,821 (GRCm39)	H317L	possibly damaging	Het
Il36b	G	A	2: 24,044,661 (GRCm39)	M20I	probably benign	Het
Ilf3	T	A	9: 21,306,168 (GRCm39)	N276K	probably damaging	Het
Jchain	T	C	5: 88,670,376 (GRCm39)	N81S	probably benign	Het
Mei4	A	G	9: 81,907,600 (GRCm39)	Y211C	probably damaging	Het
Mx2	A	T	16: 97,339,904 (GRCm39)	E20V	possibly damaging	Het
Ndufa10	A	G	1: 92,388,096 (GRCm39)		probably null	Het
Nodal	A	G	10: 61,259,456 (GRCm39)	T298A	probably damaging	Het
Nvl	T	C	1: 180,966,863 (GRCm39)	Y47C	probably damaging	Het
Onecut1	C	T	9: 74,770,674 (GRCm39)	R366C	probably damaging	Het
Or9q1	T	C	19: 13,804,983 (GRCm39)	Y259C	probably damaging	Het
Plekha6	G	C	1: 133,200,045 (GRCm39)	R208P	possibly damaging	Het
Ppp5c	A	G	7: 16,761,616 (GRCm39)		probably null	Het
Prag1	A	G	8: 36,570,641 (GRCm39)	E408G	probably benign	Het
Ptpn21	T	C	12: 98,648,809 (GRCm39)	N949S	probably damaging	Het
Scn7a	A	C	2: 66,527,912 (GRCm39)	N859K	possibly damaging	Het
Smarca4	T	C	9: 21,597,322 (GRCm39)	I1362T	probably damaging	Het
Syt12	T	C	19: 4,501,022 (GRCm39)	R343G	possibly damaging	Het
Topbp1	T	C	9: 103,200,727 (GRCm39)	S587P	probably benign	Het
Trim80	C	T	11: 115,337,301 (GRCm39)	Q388*	probably null	Het
Tsc2	T	C	17: 24,818,861 (GRCm39)		probably null	Het
Vmn2r130	T	A	17: 23,280,461 (GRCm39)	S41T	probably benign	Het
Xirp2	A	G	2: 67,340,006 (GRCm39)	E749G	probably damaging	Het
Zbtb24	G	A	10: 41,327,849 (GRCm39)	G245E	probably benign	Het
Zfp507	A	G	7: 35,487,167 (GRCm39)	V767A	probably benign	Het

Other mutations in Vmn2r3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01019:Vmn2r3	APN	3	64,167,304 (GRCm39)	missense	probably damaging	1.00
IGL01468:Vmn2r3	APN	3	64,182,382 (GRCm39)	missense	possibly damaging	0.57
IGL02032:Vmn2r3	APN	3	64,182,476 (GRCm39)	missense	possibly damaging	0.95
IGL02405:Vmn2r3	APN	3	64,178,620 (GRCm39)	splice site	probably benign
IGL02640:Vmn2r3	APN	3	64,194,816 (GRCm39)	missense	probably benign
IGL02719:Vmn2r3	APN	3	64,183,031 (GRCm39)	missense	probably damaging	1.00
IGL02746:Vmn2r3	APN	3	64,167,239 (GRCm39)	missense	possibly damaging	0.74
IGL02952:Vmn2r3	APN	3	64,186,256 (GRCm39)	missense	probably damaging	1.00
IGL03390:Vmn2r3	APN	3	64,182,767 (GRCm39)	missense	possibly damaging	0.55
G1citation:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R0023:Vmn2r3	UTSW	3	64,182,787 (GRCm39)	missense	probably damaging	0.99
R0433:Vmn2r3	UTSW	3	64,183,054 (GRCm39)	missense	possibly damaging	0.51
R0647:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R1071:Vmn2r3	UTSW	3	64,182,697 (GRCm39)	missense	possibly damaging	0.79
R1536:Vmn2r3	UTSW	3	64,182,538 (GRCm39)	missense	probably damaging	1.00
R1806:Vmn2r3	UTSW	3	64,194,810 (GRCm39)	missense	possibly damaging	0.73
R1806:Vmn2r3	UTSW	3	64,182,893 (GRCm39)	missense	probably benign	0.03
R1852:Vmn2r3	UTSW	3	64,166,815 (GRCm39)	missense	probably damaging	1.00
R1868:Vmn2r3	UTSW	3	64,166,537 (GRCm39)	missense	probably damaging	1.00
R2072:Vmn2r3	UTSW	3	64,182,493 (GRCm39)	missense	possibly damaging	0.87
R2240:Vmn2r3	UTSW	3	64,166,483 (GRCm39)	missense	probably benign	0.44
R2446:Vmn2r3	UTSW	3	64,182,733 (GRCm39)	missense	probably damaging	0.98
R4133:Vmn2r3	UTSW	3	64,183,138 (GRCm39)	missense	probably damaging	0.99
R4159:Vmn2r3	UTSW	3	64,194,850 (GRCm39)	nonsense	probably null
R4494:Vmn2r3	UTSW	3	64,182,692 (GRCm39)	missense	probably damaging	1.00
R4860:Vmn2r3	UTSW	3	64,183,022 (GRCm39)	missense	probably benign	0.00
R4895:Vmn2r3	UTSW	3	64,167,182 (GRCm39)	missense	probably benign	0.00
R4912:Vmn2r3	UTSW	3	64,166,618 (GRCm39)	missense	probably damaging	1.00
R5018:Vmn2r3	UTSW	3	64,178,774 (GRCm39)	missense	probably benign
R5033:Vmn2r3	UTSW	3	64,167,220 (GRCm39)	missense	probably benign	0.09
R5126:Vmn2r3	UTSW	3	64,166,740 (GRCm39)	missense	probably damaging	1.00
R5148:Vmn2r3	UTSW	3	64,186,247 (GRCm39)	missense	probably damaging	1.00
R5414:Vmn2r3	UTSW	3	64,166,978 (GRCm39)	nonsense	probably null
R5905:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R5992:Vmn2r3	UTSW	3	64,167,068 (GRCm39)	missense	probably damaging	1.00
R6028:Vmn2r3	UTSW	3	64,182,698 (GRCm39)	missense	probably benign	0.19
R6331:Vmn2r3	UTSW	3	64,186,182 (GRCm39)	missense	probably damaging	1.00
R6378:Vmn2r3	UTSW	3	64,182,517 (GRCm39)	missense	probably damaging	1.00
R6775:Vmn2r3	UTSW	3	64,183,039 (GRCm39)	missense	possibly damaging	0.88
R6822:Vmn2r3	UTSW	3	64,194,876 (GRCm39)	missense	probably benign	0.30
R6826:Vmn2r3	UTSW	3	64,182,327 (GRCm39)	nonsense	probably null
R6886:Vmn2r3	UTSW	3	64,166,927 (GRCm39)	missense	probably damaging	1.00
R6971:Vmn2r3	UTSW	3	64,166,668 (GRCm39)	missense	probably damaging	0.99
R7154:Vmn2r3	UTSW	3	64,194,732 (GRCm39)	missense	probably benign	0.02
R7192:Vmn2r3	UTSW	3	64,167,364 (GRCm39)	missense	probably benign	0.24
R7282:Vmn2r3	UTSW	3	64,168,825 (GRCm39)	missense	possibly damaging	0.90
R7472:Vmn2r3	UTSW	3	64,182,953 (GRCm39)	missense	probably benign	0.00
R7563:Vmn2r3	UTSW	3	64,182,770 (GRCm39)	missense	possibly damaging	0.60
R7726:Vmn2r3	UTSW	3	64,182,939 (GRCm39)	nonsense	probably null
R7966:Vmn2r3	UTSW	3	64,186,235 (GRCm39)	missense	probably damaging	0.99
R8025:Vmn2r3	UTSW	3	64,182,871 (GRCm39)	missense	possibly damaging	0.91
R8050:Vmn2r3	UTSW	3	64,178,714 (GRCm39)	missense	probably damaging	0.99
R8300:Vmn2r3	UTSW	3	64,182,347 (GRCm39)	missense	probably benign	0.00
R8402:Vmn2r3	UTSW	3	64,178,617 (GRCm39)	splice site	probably benign
R8486:Vmn2r3	UTSW	3	64,186,370 (GRCm39)	missense	probably damaging	1.00
R8523:Vmn2r3	UTSW	3	64,182,311 (GRCm39)	missense	probably benign	0.03
R8678:Vmn2r3	UTSW	3	64,166,896 (GRCm39)	missense	possibly damaging	0.76
R8885:Vmn2r3	UTSW	3	64,182,383 (GRCm39)	missense	probably benign	0.00
R8886:Vmn2r3	UTSW	3	64,194,892 (GRCm39)	missense	possibly damaging	0.47
R8905:Vmn2r3	UTSW	3	64,166,695 (GRCm39)	missense	probably damaging	0.99
R8937:Vmn2r3	UTSW	3	64,166,673 (GRCm39)	missense	probably damaging	1.00
R8955:Vmn2r3	UTSW	3	64,168,803 (GRCm39)	missense	possibly damaging	0.56
R9172:Vmn2r3	UTSW	3	64,186,403 (GRCm39)	missense	possibly damaging	0.79
R9485:Vmn2r3	UTSW	3	64,183,046 (GRCm39)	missense	probably damaging	1.00
R9575:Vmn2r3	UTSW	3	64,178,735 (GRCm39)	missense	probably benign	0.01
R9618:Vmn2r3	UTSW	3	64,178,724 (GRCm39)	missense	probably damaging	0.98
X0022:Vmn2r3	UTSW	3	64,182,389 (GRCm39)	missense	probably damaging	1.00
X0022:Vmn2r3	UTSW	3	64,178,669 (GRCm39)	missense	possibly damaging	0.62

Predicted Primers

PCR Primer
(F):5'- GGAATGCAACATCCCTTTGAG -3'
(R):5'- GGCAAATTCAAAGTGGCTGTG -3'

Sequencing Primer
(F):5'- TGAGCTTTTCCTTATCATTTCAGAG -3'
(R):5'- CAAAGTGGCTGTGGAAATATTTGCC -3'

Posted On

2016-12-15