|Institutional Source||Beutler Lab|
|Gene Name||dickkopf WNT signaling pathway inhibitor 4|
|Is this an essential gene?||Non essential (E-score: 0.000)|
|Stock #||R5788 (G1)|
|Chromosomal Location||22624043-22627547 bp(+) (GRCm38)|
|Type of Mutation||missense|
|DNA Base Change (assembly)||T to C at 22625331 bp|
|Amino Acid Change||Cysteine to Arginine at position 66 (C66R)|
|Ref Sequence||ENSEMBL: ENSMUSP00000033936 (fasta)|
|Gene Model||predicted gene model for transcript(s): [ENSMUST00000033936] [ENSMUST00000033938]|
|Predicted Effect||probably damaging
AA Change: C66R
PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
AA Change: C66R
|Predicted Effect||probably benign
|Meta Mutation Damage Score||0.9067|
|Coding Region Coverage||
|Validation Efficiency||98% (62/63)|
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a conditional ready allele are phenotypically normal. [provided by MGI curators]
|Allele List at MGI|
|Other mutations in this stock||
|Other mutations in Dkk4||
(F):5'- AGCTTGATGCACTCTTTTCAGGAG -3'
(R):5'- TCCCAAACATCCTAGCCTGG -3'
(F):5'- TGGCAATCCCACTGTCATTAGAG -3'
(R):5'- TGGCCCCCATGGTCAGTG -3'