Mutagenetix > Incidental Mutation

Incidental Mutation 'R0546:Or14j7'

44876

Institutional Source

Beutler Lab

Gene Symbol

Or14j7

Ensembl Gene

ENSMUSG00000059030

Gene Name

olfactory receptor family 14 subfamily J member 7

Synonyms

MOR218-13, GA_x6K02T2PSCP-2374126-2375048, Olfr128

MMRRC Submission

038738-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R0546 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

38234459-38235385 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

T to A at 38235229 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Cysteine to Stop codon at position 257 (C257*)

Ref Sequence

ENSEMBL: ENSMUSP00000151065 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000080231] [ENSMUST00000216440]

AlphaFold

Q7TRJ1

Predicted Effect

probably null
Transcript: ENSMUST00000080231
AA Change: C257*

SMART Domains

Protein: ENSMUSP00000079121
Gene: ENSMUSG00000059030
AA Change: C257*

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.3e-46	PFAM
Pfam:7TM_GPCR_Srsx	35	305	1.1e-5	PFAM
Pfam:7tm_1	41	290	1.6e-21	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000216440
AA Change: C257*

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.1%
20x: 95.0%

Validation Efficiency

99% (74/75)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 75 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aass	A	G	6: 23,077,076 (GRCm39)		probably null	Het
Actn1	C	T	12: 80,225,208 (GRCm39)	R418Q	probably benign	Het
Adam39	G	A	8: 41,279,468 (GRCm39)	V620M	probably damaging	Het
Agr3	C	A	12: 35,978,329 (GRCm39)	T14K	probably benign	Het
Alpk2	T	C	18: 65,439,788 (GRCm39)	D1002G	probably benign	Het
Amz2	T	A	11: 109,324,780 (GRCm39)	N221K	probably benign	Het
Aox4	A	G	1: 58,289,333 (GRCm39)	E752G	probably damaging	Het
Ap2a1	C	T	7: 44,554,132 (GRCm39)	G500S	probably damaging	Het
Car5b	G	A	X: 162,762,297 (GRCm39)	R282C	probably damaging	Het
Ccdc112	A	G	18: 46,424,139 (GRCm39)	S200P	possibly damaging	Het
Ccdc18	A	T	5: 108,322,830 (GRCm39)	E643D	probably benign	Het
Ccdc180	A	T	4: 45,904,597 (GRCm39)	T398S	possibly damaging	Het
Cnp	A	G	11: 100,471,549 (GRCm39)	Y397C	probably damaging	Het
Cpa4	T	C	6: 30,580,962 (GRCm39)	W184R	probably damaging	Het
Crebbp	A	T	16: 3,903,671 (GRCm39)	I1856N	probably damaging	Het
Ctrl	A	G	8: 106,658,966 (GRCm39)	I200T	probably damaging	Het
Cyfip1	C	T	7: 55,572,564 (GRCm39)	R934*	probably null	Het
Dennd5a	A	G	7: 109,520,633 (GRCm39)	V408A	probably benign	Het
Dhfr	G	A	13: 92,504,692 (GRCm39)		probably null	Het
Dnajc6	A	T	4: 101,492,388 (GRCm39)	N740Y	probably damaging	Het
Fam110a	T	C	2: 151,812,732 (GRCm39)	T13A	probably benign	Het
Fars2	G	T	13: 36,388,569 (GRCm39)	K19N	probably benign	Het
Fer1l6	A	T	15: 58,430,257 (GRCm39)		probably null	Het
Gabra1	T	A	11: 42,053,428 (GRCm39)	T69S	probably damaging	Het
Galnt18	T	G	7: 111,107,348 (GRCm39)	N475T	probably damaging	Het
Gbp4	T	C	5: 105,268,836 (GRCm39)	Y439C	probably damaging	Het
Gpatch2l	A	G	12: 86,335,622 (GRCm39)	*409W	probably null	Het
Hip1r	T	C	5: 124,137,114 (GRCm39)	V658A	possibly damaging	Het
Hspg2	A	G	4: 137,229,605 (GRCm39)	D73G	probably benign	Het
Ifitm2	A	G	7: 140,535,656 (GRCm39)	V58A	possibly damaging	Het
Ift172	T	C	5: 31,414,945 (GRCm39)	D1359G	probably benign	Het
Ing1	A	G	8: 11,607,031 (GRCm39)	D41G	probably damaging	Het
Itgal	C	T	7: 126,909,486 (GRCm39)	T446I	probably benign	Het
Itgav	G	T	2: 83,633,586 (GRCm39)	M978I	probably benign	Het
Jazf1	A	G	6: 52,754,681 (GRCm39)	Y132H	possibly damaging	Het
Lgr4	T	A	2: 109,829,766 (GRCm39)	N211K	probably damaging	Het
Mgat4d	A	G	8: 84,082,350 (GRCm39)	N100S	possibly damaging	Het
Mrgprb3	T	C	7: 48,293,263 (GRCm39)	Y96C	probably damaging	Het
Myh11	A	C	16: 14,023,492 (GRCm39)	L1562R	probably damaging	Het
Mylk	G	C	16: 34,699,845 (GRCm39)	E403Q	possibly damaging	Het
Myo15a	A	G	11: 60,397,139 (GRCm39)	Y2667C	probably damaging	Het
Or52ae7	A	G	7: 103,119,907 (GRCm39)	I220M	possibly damaging	Het
Or6c70	G	A	10: 129,710,407 (GRCm39)	T73I	possibly damaging	Het
Or8k37	T	A	2: 86,469,573 (GRCm39)	T160S	possibly damaging	Het
Or8k38	C	T	2: 86,488,235 (GRCm39)	C189Y	possibly damaging	Het
Or9m2	T	A	2: 87,820,816 (GRCm39)	Y120*	probably null	Het
Paox	G	T	7: 139,711,591 (GRCm39)	G148W	probably damaging	Het
Pkd1	T	C	17: 24,799,112 (GRCm39)	V2777A	probably benign	Het
Plod2	T	A	9: 92,477,388 (GRCm39)	V360E	probably damaging	Het
Prune2	T	C	19: 16,998,030 (GRCm39)		probably benign	Het
Sbds	G	T	5: 130,282,919 (GRCm39)	A3D	possibly damaging	Het
Sec23a	T	C	12: 59,031,953 (GRCm39)	T426A	probably benign	Het
Sec31a	T	C	5: 100,551,929 (GRCm39)	Y148C	probably damaging	Het
Shprh	T	A	10: 11,059,631 (GRCm39)		probably benign	Het
Slc16a7	A	G	10: 125,066,742 (GRCm39)	V299A	probably benign	Het
Smg8	T	C	11: 86,974,439 (GRCm39)	Y174C	possibly damaging	Het
Snx22	T	A	9: 65,976,059 (GRCm39)	Y58F	probably damaging	Het
Snx25	A	G	8: 46,556,667 (GRCm39)	Y308H	probably benign	Het
St3gal2	A	G	8: 111,696,738 (GRCm39)		probably null	Het
Stab1	C	T	14: 30,861,507 (GRCm39)	R2500H	possibly damaging	Het
Steap4	T	C	5: 8,025,870 (GRCm39)	S144P	probably damaging	Het
Stfa3	T	A	16: 36,272,619 (GRCm39)		probably benign	Het
Tmprss9	C	A	10: 80,735,157 (GRCm39)	Q1095K	probably benign	Het
Top2a	A	G	11: 98,890,052 (GRCm39)	V1217A	possibly damaging	Het
Trhr2	A	G	8: 123,085,228 (GRCm39)		probably null	Het
Trim7	A	G	11: 48,736,336 (GRCm39)	E23G	probably damaging	Het
Trpv3	A	T	11: 73,188,013 (GRCm39)	E788V	probably damaging	Het
Ttn	A	G	2: 76,575,863 (GRCm39)	I25010T	probably damaging	Het
Ube2ql1	T	C	13: 69,887,419 (GRCm39)	H14R	unknown	Het
Uggt1	C	T	1: 36,235,052 (GRCm39)	R419H	probably benign	Het
Xpo4	A	G	14: 57,850,731 (GRCm39)	V391A	probably benign	Het
Zfhx3	A	G	8: 109,520,819 (GRCm39)	D647G	probably damaging	Het
Zfp354c	A	T	11: 50,706,457 (GRCm39)	M206K	probably benign	Het
Zfp804a	A	G	2: 82,089,264 (GRCm39)	N1031S	possibly damaging	Het
Zfp868	A	G	8: 70,064,882 (GRCm39)	V151A	probably benign	Het

Other mutations in Or14j7

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01922:Or14j7	APN	17	38,234,850 (GRCm39)	missense	possibly damaging	0.73
IGL01940:Or14j7	APN	17	38,235,177 (GRCm39)	missense	probably damaging	1.00
R0427:Or14j7	UTSW	17	38,234,520 (GRCm39)	missense	probably benign	0.00
R0462:Or14j7	UTSW	17	38,234,667 (GRCm39)	missense	probably damaging	1.00
R0760:Or14j7	UTSW	17	38,235,005 (GRCm39)	nonsense	probably null
R0940:Or14j7	UTSW	17	38,234,591 (GRCm39)	missense	probably damaging	0.98
R1898:Or14j7	UTSW	17	38,234,516 (GRCm39)	missense	possibly damaging	0.91
R1935:Or14j7	UTSW	17	38,234,993 (GRCm39)	missense	probably damaging	1.00
R2060:Or14j7	UTSW	17	38,234,771 (GRCm39)	missense	probably benign	0.00
R4680:Or14j7	UTSW	17	38,234,813 (GRCm39)	missense	probably damaging	1.00
R5165:Or14j7	UTSW	17	38,235,252 (GRCm39)	missense	probably benign	0.14
R5526:Or14j7	UTSW	17	38,235,383 (GRCm39)	makesense	probably null
R5594:Or14j7	UTSW	17	38,234,502 (GRCm39)	missense	probably benign	0.00
R5751:Or14j7	UTSW	17	38,234,861 (GRCm39)	missense	probably benign	0.44
R6330:Or14j7	UTSW	17	38,234,685 (GRCm39)	missense	possibly damaging	0.77
R7123:Or14j7	UTSW	17	38,234,567 (GRCm39)	missense	probably benign	0.33
R7354:Or14j7	UTSW	17	38,235,284 (GRCm39)	missense	probably benign	0.00
R7827:Or14j7	UTSW	17	38,234,568 (GRCm39)	nonsense	probably null
R8258:Or14j7	UTSW	17	38,234,847 (GRCm39)	missense	probably damaging	1.00
R8259:Or14j7	UTSW	17	38,234,847 (GRCm39)	missense	probably damaging	1.00
R8686:Or14j7	UTSW	17	38,235,168 (GRCm39)	missense	probably benign	0.00
R8787:Or14j7	UTSW	17	38,235,075 (GRCm39)	missense	probably benign	0.06
R9036:Or14j7	UTSW	17	38,235,168 (GRCm39)	missense	probably benign	0.03
R9278:Or14j7	UTSW	17	38,235,275 (GRCm39)	missense	probably benign
R9761:Or14j7	UTSW	17	38,234,934 (GRCm39)	missense	probably benign	0.03
Z1177:Or14j7	UTSW	17	38,234,618 (GRCm39)	missense	probably benign	0.02

Predicted Primers

PCR Primer
(F):5'- TGCTGTAATGGGATTTGCCTGCTTTA -3'
(R):5'- GTGTGGCAACAGACTGGAAGAGTTAG -3'

Sequencing Primer
(F):5'- ATGGGGATTGCCTTCTCCTATG -3'
(R):5'- aggagacagaggtaggcag -3'

Posted On

2013-06-11