Mutagenetix > Incidental Mutation

Incidental Mutation 'R5808:Tmem167b'

448827

Institutional Source

Beutler Lab

Gene Symbol

Tmem167b

Ensembl Gene

ENSMUSG00000068732

Gene Name

transmembrane protein 167B

Synonyms

2010200O16Rik

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.275) question?

Stock #

R5808 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

108463741-108469782 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 108467559 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Histidine at position 29 (R29H)

Ref Sequence

ENSEMBL: ENSMUSP00000088035 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000090546] [ENSMUST00000106622]

AlphaFold

Q80X45

Predicted Effect

probably benign
Transcript: ENSMUST00000090546
AA Change: R29H

PolyPhen 2 Score 0.267 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000088035
Gene: ENSMUSG00000068732
AA Change: R29H

Domain	Start	End	E-Value	Type
Pfam:DUF1242	10	46	7.6e-14	PFAM
transmembrane domain	50	72	N/A	INTRINSIC

Predicted Effect

silent
Transcript: ENSMUST00000106622

SMART Domains

Protein: ENSMUSP00000102233
Gene: ENSMUSG00000068732

Domain	Start	End	E-Value	Type
signal peptide	1	39	N/A	INTRINSIC
low complexity region	58	78	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000199996

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.2%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 58 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2610028H24Rik	C	T	10: 76,286,482 (GRCm39)	R6W	probably damaging	Het
Ahnak	T	A	19: 8,987,599 (GRCm39)	V2961E	possibly damaging	Het
Aldh1a7	A	T	19: 20,685,561 (GRCm39)	Y330N	possibly damaging	Het
Bag6	A	G	17: 35,365,298 (GRCm39)	Y1041C	probably damaging	Het
Baz1b	T	A	5: 135,250,812 (GRCm39)	V866E	probably benign	Het
Bmpr2	A	T	1: 59,906,560 (GRCm39)	Y551F	probably benign	Het
Cage1	A	G	13: 38,206,301 (GRCm39)		probably benign	Het
Caskin2	G	T	11: 115,692,589 (GRCm39)	P732Q	probably damaging	Het
Cbfa2t2	T	C	2: 154,359,746 (GRCm39)		probably null	Het
Ccdc162	A	T	10: 41,531,500 (GRCm39)	M502K	possibly damaging	Het
Cfap157	T	C	2: 32,670,657 (GRCm39)	D197G	probably damaging	Het
CN725425	T	A	15: 91,129,847 (GRCm39)	S237T	probably benign	Het
Defb40	T	C	8: 19,025,095 (GRCm39)	H37R	probably benign	Het
Dis3l2	A	C	1: 86,977,360 (GRCm39)	S836R	possibly damaging	Het
Dnah14	T	C	1: 181,568,724 (GRCm39)	I2818T	possibly damaging	Het
Dpysl2	T	A	14: 67,102,621 (GRCm39)		probably null	Het
Dsc1	A	T	18: 20,219,886 (GRCm39)	C761*	probably null	Het
Dsg1b	A	T	18: 20,541,782 (GRCm39)	D763V	probably damaging	Het
Eif2ak1	C	T	5: 143,820,812 (GRCm39)	P251L	probably benign	Het
Epha6	A	T	16: 59,503,105 (GRCm39)	I934K	probably damaging	Het
Fbxo40	T	C	16: 36,790,744 (GRCm39)	E122G	probably damaging	Het
Filip1	C	T	9: 79,725,983 (GRCm39)	G879R	possibly damaging	Het
Frem2	A	T	3: 53,559,984 (GRCm39)	F1508I	probably damaging	Het
Gja1	A	G	10: 56,264,594 (GRCm39)	N318D	probably benign	Het
Gm11564	T	C	11: 99,705,867 (GRCm39)	S188G	unknown	Het
Hlcs	A	G	16: 94,063,491 (GRCm39)	V523A	probably benign	Het
Kif1a	C	A	1: 92,970,420 (GRCm39)	E1041D	probably damaging	Het
Lamtor1	A	G	7: 101,559,289 (GRCm39)	Y81C	possibly damaging	Het
Loxl1	A	G	9: 58,201,732 (GRCm39)	L453S	probably damaging	Het
Mcf2l	A	G	8: 13,043,937 (GRCm39)	M1V	probably null	Het
Megf6	A	G	4: 154,352,119 (GRCm39)	Q1208R	probably benign	Het
Morc2a	T	A	11: 3,633,781 (GRCm39)	I631N	probably benign	Het
Muc6	T	C	7: 141,226,360 (GRCm39)		probably benign	Het
Mybpc1	T	C	10: 88,406,428 (GRCm39)	S139G	possibly damaging	Het
Myo18a	T	C	11: 77,720,127 (GRCm39)	F1017L	probably benign	Het
Nup62	A	C	7: 44,479,416 (GRCm39)	Q477P	possibly damaging	Het
P2ry13	T	A	3: 59,117,653 (GRCm39)	I42F	probably benign	Het
Ptprb	G	A	10: 116,175,392 (GRCm39)	R1129K	probably benign	Het
Rexo4	T	A	2: 26,854,197 (GRCm39)	K45I	probably damaging	Het
Rnf213	G	A	11: 119,327,121 (GRCm39)	V1703I	probably benign	Het
Sec23ip	C	A	7: 128,373,908 (GRCm39)	A710E	probably benign	Het
Sfi1	A	ATCTTCCCAAAGCCAGTGC	11: 3,103,384 (GRCm39)		probably benign	Het
Slc41a2	T	C	10: 83,149,362 (GRCm39)	T194A	probably benign	Het
Slc4a10	G	A	2: 62,080,816 (GRCm39)	A348T	probably damaging	Het
Soat2	G	A	15: 102,062,460 (GRCm39)		probably null	Het
Srms	T	C	2: 180,850,548 (GRCm39)	S204G	probably benign	Het
Szt2	G	A	4: 118,229,810 (GRCm39)	R2774C	unknown	Het
Tcf7l2	T	C	19: 55,896,973 (GRCm39)	V182A	probably damaging	Het
Trappc12	T	A	12: 28,796,863 (GRCm39)	D223V	probably damaging	Het
Ttc6	T	A	12: 57,664,397 (GRCm39)	S383R	possibly damaging	Het
Ubr1	C	T	2: 120,791,573 (GRCm39)	R137H	possibly damaging	Het
Vmn2r24	T	A	6: 123,792,597 (GRCm39)	C641*	probably null	Het
Vrk3	A	G	7: 44,409,298 (GRCm39)	D155G	probably damaging	Het
Xxylt1	T	C	16: 30,869,503 (GRCm39)	Y199C	probably damaging	Het
Zbtb46	A	T	2: 181,065,363 (GRCm39)	D262E	probably benign	Het
Zcchc9	A	T	13: 91,948,766 (GRCm39)	S58T	probably benign	Het
Zfp747l1	A	T	7: 126,984,085 (GRCm39)		probably benign	Het
Zfyve16	A	T	13: 92,631,563 (GRCm39)	L1344*	probably null	Het

Other mutations in Tmem167b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01542:Tmem167b	APN	3	108,466,222 (GRCm39)	missense	possibly damaging	0.82
R0847:Tmem167b	UTSW	3	108,467,537 (GRCm39)	missense	probably benign
R5310:Tmem167b	UTSW	3	108,469,415 (GRCm39)	intron	probably benign
R5524:Tmem167b	UTSW	3	108,467,569 (GRCm39)	missense	possibly damaging	0.84
R6093:Tmem167b	UTSW	3	108,469,439 (GRCm39)	start codon destroyed	probably null
R6358:Tmem167b	UTSW	3	108,466,211 (GRCm39)	missense	possibly damaging	0.94
R7716:Tmem167b	UTSW	3	108,466,213 (GRCm39)	missense	probably damaging	1.00
R8922:Tmem167b	UTSW	3	108,467,541 (GRCm39)	missense	probably benign	0.05

Predicted Primers

PCR Primer
(F):5'- CAGGCGGATCATAAACGTTAGTG -3'
(R):5'- TTGGGGAAACAGTGCCAAGATC -3'

Sequencing Primer
(F):5'- GATCATAAACGTTAGTGGAAATTCCG -3'
(R):5'- GTGCCAAGATCATGGACCCTTC -3'

Posted On

2016-12-15