Mutagenetix > Incidental Mutation

Incidental Mutation 'R5819:Mov10'

449208

Institutional Source

Beutler Lab

Gene Symbol

Mov10

Ensembl Gene

ENSMUSG00000002227

Gene Name

Moloney leukemia virus 10

Synonyms

Mov-10

MMRRC Submission

043399-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.381) question?

Stock #

R5819 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

104794836-104818563 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 104801512 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 395 (G395D)

Ref Sequence

ENSEMBL: ENSMUSP00000128246 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000002297] [ENSMUST00000106774] [ENSMUST00000106775] [ENSMUST00000136148] [ENSMUST00000166979] [ENSMUST00000168015]

AlphaFold

P23249

Predicted Effect

probably damaging
Transcript: ENSMUST00000002297
AA Change: G395D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000002297
Gene: ENSMUSG00000002227
AA Change: G395D

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000106774

SMART Domains

Protein: ENSMUSP00000102386
Gene: ENSMUSG00000002227

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000106775
AA Change: G468D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000102387
Gene: ENSMUSG00000002227
AA Change: G468D

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000136148

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000145414

Predicted Effect

probably damaging
Transcript: ENSMUST00000166979
AA Change: G468D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000126897
Gene: ENSMUSG00000002227
AA Change: G468D

Domain	Start	End	E-Value	Type
low complexity region	61	75	N/A	INTRINSIC
low complexity region	370	385	N/A	INTRINSIC
low complexity region	411	426	N/A	INTRINSIC
AAA	590	772	5.72e-3	SMART
low complexity region	1026	1043	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000168015
AA Change: G395D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128246
Gene: ENSMUSG00000002227
AA Change: G395D

Domain	Start	End	E-Value	Type
low complexity region	297	312	N/A	INTRINSIC
low complexity region	338	353	N/A	INTRINSIC
AAA	517	699	5.72e-3	SMART
low complexity region	953	970	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196161

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196211

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.3%
20x: 95.2%

Validation Efficiency

MGI Phenotype

PHENOTYPE: Homozygous knockout is embryonic lethal. Heterozygous knockout leads to reduced dendritic branching of neurons, which affects anxiety- and/or activity-related behavior. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4932438A13Rik	A	T	3: 37,048,600	M4863L	probably benign	Het
Abca4	A	T	3: 122,136,981	I1376F	probably damaging	Het
Aggf1	T	C	13: 95,351,621	N673D	possibly damaging	Het
Avil	T	C	10: 127,009,998	F372S	probably damaging	Het
Bod1l	T	A	5: 41,832,605	E258D	probably benign	Het
Ccdc129	A	T	6: 55,897,891	K275N	probably benign	Het
Chek1	T	A	9: 36,710,405	H420L	probably benign	Het
Cyfip1	A	G	7: 55,879,151	I260M	probably damaging	Het
Dclk1	G	A	3: 55,489,864	V524I	probably damaging	Het
Efr3b	T	C	12: 3,992,965	M102V	probably benign	Het
Erc2	T	A	14: 28,141,369	I517N	probably damaging	Het
Fubp1	T	C	3: 152,220,553	I305T	probably damaging	Het
Galc	T	A	12: 98,216,261	D443V	probably benign	Het
Galnt4	T	A	10: 99,110,030	I539N	probably damaging	Het
Gm17093	G	T	14: 44,521,529	M169I	unknown	Het
Gm4858	A	G	3: 93,073,732	Y19C	probably damaging	Het
Htra1	T	C	7: 130,981,739	F363S	probably damaging	Het
Klhdc8b	G	A	9: 108,451,062	P64S	probably benign	Het
Kmt2c	A	G	5: 25,409,132		probably null	Het
Mettl21c	T	G	1: 44,009,722	K222Q	probably damaging	Het
Mga	A	C	2: 119,941,263	M1535L	possibly damaging	Het
Ms4a10	C	A	19: 10,968,690	A26S	probably benign	Het
Naaladl1	A	G	19: 6,109,654	N372D	possibly damaging	Het
Olfr1275	A	T	2: 111,230,959	I278N	probably damaging	Het
Optc	T	C	1: 133,897,879	D303G	probably damaging	Het
Osmr	C	A	15: 6,815,787	V833L	probably benign	Het
Phf14	A	G	6: 11,997,252		probably null	Het
Pjvk	A	G	2: 76,658,369	I295V	probably benign	Het
Plppr4	T	A	3: 117,325,864	I299L	possibly damaging	Het
Prkg1	A	G	19: 31,585,672	S110P	probably benign	Het
Ptprq	T	A	10: 107,719,883		probably benign	Het
Rarb	T	G	14: 16,443,820	N156T	possibly damaging	Het
Rgl3	A	T	9: 21,981,602		probably null	Het
Ruvbl1	A	C	6: 88,483,115		probably null	Het
S1pr1	G	T	3: 115,712,140	C268*	probably null	Het
Sbk3	T	C	7: 4,969,997	D58G	probably benign	Het
Scgb2b3	T	A	7: 31,360,214	H45L	possibly damaging	Het
Smim8	TTTAATGAAGAGCT	TT	4: 34,771,261		probably benign	Het
Soga3	A	T	10: 29,197,273	M854L	probably benign	Het
Tas2r119	T	C	15: 32,177,306	L6P	probably damaging	Het
Tcp11	A	T	17: 28,069,236	F339L	probably damaging	Het
Tmprss5	T	A	9: 49,114,479		probably null	Het
Trnau1ap	A	G	4: 132,325,210		probably benign	Het
Trp53bp1	G	A	2: 121,208,392	R1397*	probably null	Het
Ubqln3	G	A	7: 104,141,467	P472L	probably benign	Het
Vmn2r13	T	A	5: 109,174,100	M244L	possibly damaging	Het
Zfp777	C	T	6: 48,037,588	E395K	probably damaging	Het
Zfyve27	A	G	19: 42,183,496	S156G	probably benign	Het

Other mutations in Mov10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00498:Mov10	APN	3	104800947	splice site	probably benign
IGL01111:Mov10	APN	3	104801405	missense	possibly damaging	0.71
IGL01315:Mov10	APN	3	104795945	missense	probably damaging	0.98
IGL01463:Mov10	APN	3	104800324	missense	probably damaging	1.00
IGL02114:Mov10	APN	3	104795318	unclassified	probably benign
IGL02354:Mov10	APN	3	104804121	splice site	probably benign
IGL02361:Mov10	APN	3	104804121	splice site	probably benign
IGL02692:Mov10	APN	3	104800803	nonsense	probably null
IGL03104:Mov10	APN	3	104797307	missense	probably damaging	1.00
IGL03121:Mov10	APN	3	104801002	missense	probably benign
P0040:Mov10	UTSW	3	104804679	missense	probably damaging	1.00
R0025:Mov10	UTSW	3	104804603	missense	probably damaging	1.00
R0270:Mov10	UTSW	3	104795405	missense	probably benign	0.09
R0747:Mov10	UTSW	3	104802496	missense	probably benign	0.41
R1434:Mov10	UTSW	3	104795174	missense	probably damaging	1.00
R1482:Mov10	UTSW	3	104804546	missense	probably damaging	0.98
R1594:Mov10	UTSW	3	104795411	missense	probably damaging	1.00
R1656:Mov10	UTSW	3	104799596	missense	probably benign	0.03
R1739:Mov10	UTSW	3	104800282	missense	probably damaging	0.98
R1785:Mov10	UTSW	3	104818116	missense	possibly damaging	0.73
R1786:Mov10	UTSW	3	104818116	missense	possibly damaging	0.73
R1911:Mov10	UTSW	3	104801560	splice site	probably benign
R1962:Mov10	UTSW	3	104796977	missense	probably damaging	1.00
R1993:Mov10	UTSW	3	104799419	missense	probably damaging	1.00
R2095:Mov10	UTSW	3	104801531	missense	probably damaging	1.00
R2138:Mov10	UTSW	3	104804242	missense	probably benign	0.00
R3107:Mov10	UTSW	3	104799724	missense	probably damaging	1.00
R4241:Mov10	UTSW	3	104797276	missense	probably benign	0.45
R4280:Mov10	UTSW	3	104799779	missense	probably damaging	0.98
R4474:Mov10	UTSW	3	104818465	missense	probably damaging	1.00
R5227:Mov10	UTSW	3	104802578	missense	probably benign
R5391:Mov10	UTSW	3	104802533	missense	probably benign	0.12
R5704:Mov10	UTSW	3	104799596	missense	probably benign	0.03
R5842:Mov10	UTSW	3	104799379	splice site	probably benign
R6059:Mov10	UTSW	3	104817950	utr 3 prime	probably benign
R6692:Mov10	UTSW	3	104818044	missense	probably damaging	0.97
R7226:Mov10	UTSW	3	104801012	missense	probably damaging	1.00
R7426:Mov10	UTSW	3	104800052	splice site	probably null
R7633:Mov10	UTSW	3	104797065	missense	possibly damaging	0.93
R7637:Mov10	UTSW	3	104795885	missense	probably benign	0.26
R7869:Mov10	UTSW	3	104804678	missense	probably damaging	1.00
R8684:Mov10	UTSW	3	104804374	missense	probably benign
R9008:Mov10	UTSW	3	104800016	missense	probably benign	0.09
R9127:Mov10	UTSW	3	104804343	nonsense	probably null
R9559:Mov10	UTSW	3	104800961	missense
R9587:Mov10	UTSW	3	104804583	missense	probably benign	0.11
R9602:Mov10	UTSW	3	104800968	missense	probably benign	0.18
R9606:Mov10	UTSW	3	104800348	missense	probably benign	0.00
R9708:Mov10	UTSW	3	104797297	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CTCTGAAAGCAAGACCTCCTATG -3'
(R):5'- ACTGGCCAAGAAGCAGCTAG -3'

Sequencing Primer
(F):5'- AAGACCTCCTATGCCCAGGG -3'
(R):5'- TGGGGACATTGGCACCACTG -3'

Posted On

2016-12-20