Mutagenetix > Incidental Mutation

Incidental Mutation 'R5823:Tex15'

450037

Institutional Source

Beutler Lab

Gene Symbol

Tex15

Ensembl Gene

ENSMUSG00000009628

Gene Name

testis expressed gene 15

Synonyms

2210014E14Rik

MMRRC Submission

043215-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.241) question?

Stock #

R5823 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

33516738-33585582 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 33570934 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 405 (I405V)

Ref Sequence

ENSEMBL: ENSMUSP00000120744 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000009772] [ENSMUST00000124496] [ENSMUST00000124501]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000009772
AA Change: I131V

PolyPhen 2 Score 0.422 (Sensitivity: 0.89; Specificity: 0.90)

SMART Domains

Protein: ENSMUSP00000009772
Gene: ENSMUSG00000009628
AA Change: I131V

Domain	Start	End	E-Value	Type
low complexity region	262	274	N/A	INTRINSIC
low complexity region	302	313	N/A	INTRINSIC
low complexity region	524	536	N/A	INTRINSIC
low complexity region	665	674	N/A	INTRINSIC
low complexity region	713	725	N/A	INTRINSIC
low complexity region	946	961	N/A	INTRINSIC
low complexity region	1497	1508	N/A	INTRINSIC
Pfam:TEX15	1572	1788	1.3e-109	PFAM
Pfam:TEX15	1901	2119	1.1e-16	PFAM
low complexity region	2758	2770	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000124496
AA Change: I405V

PolyPhen 2 Score 0.670 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000120744
Gene: ENSMUSG00000009628
AA Change: I405V

Domain	Start	End	E-Value	Type
Pfam:DUF3715	89	251	1.6e-58	PFAM
low complexity region	536	548	N/A	INTRINSIC
low complexity region	576	587	N/A	INTRINSIC
low complexity region	798	810	N/A	INTRINSIC
low complexity region	939	948	N/A	INTRINSIC
low complexity region	987	999	N/A	INTRINSIC
low complexity region	1220	1235	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000124501

SMART Domains

Protein: ENSMUSP00000138070
Gene: ENSMUSG00000009628

Domain	Start	End	E-Value	Type
Pfam:DUF3715	96	251	2.4e-52	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 92.7%

Validation Efficiency

MGI Phenotype

Strain: 3526165
PHENOTYPE: Male mice are infertile due to arrest of meiosis stemming from failure to repair double-strand breaks. However, female mice are fertile. [provided by MGI curators]

Allele List at MGI

All alleles(4) : Targeted(3) Gene trapped(1)

Other mutations in this stock

Total: 39 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1600015I10Rik	T	A	6: 48,930,552 (GRCm38)	I162N	probably damaging	Het
4930505A04Rik	C	T	11: 30,426,349 (GRCm38)	V173M	probably damaging	Het
Acsl3	G	T	1: 78,688,286 (GRCm38)	R143L	probably benign	Het
Ankrd11	A	T	8: 122,895,790 (GRCm38)	I420K	probably benign	Het
Cwh43	A	G	5: 73,411,870 (GRCm38)	E85G	probably benign	Het
Dnah1	G	T	14: 31,266,418 (GRCm38)	F3442L	possibly damaging	Het
Dock6	T	A	9: 21,804,828 (GRCm38)	N1737I	probably damaging	Het
Fcf1	A	G	12: 84,974,147 (GRCm38)	Y55C	possibly damaging	Het
Fer1l6	T	A	15: 58,590,503 (GRCm38)	Y802*	probably null	Het
Fgf9	T	G	14: 58,083,302 (GRCm38)	S10A	probably damaging	Het
Fhad1	A	G	4: 141,955,306 (GRCm38)	I508T	possibly damaging	Het
Flnc	T	C	6: 29,461,202 (GRCm38)	V2692A	probably damaging	Het
Gm38119	G	T	3: 92,738,073 (GRCm38)	H71Q	unknown	Het
Hcn1	A	T	13: 117,602,852 (GRCm38)	H50L	unknown	Het
Helz2	T	C	2: 181,236,396 (GRCm38)	T870A	possibly damaging	Het
Iqcf4	T	C	9: 106,568,601 (GRCm38)	I116V	probably benign	Het
Lama5	T	A	2: 180,192,492 (GRCm38)	I1383F	probably benign	Het
Lmo2	C	T	2: 103,981,072 (GRCm38)	T150I	probably damaging	Het
Mgat4f	T	A	1: 134,390,917 (GRCm38)	W476R	probably damaging	Het
Myh13	A	G	11: 67,360,468 (GRCm38)	E1391G	probably damaging	Het
Mylk	T	A	16: 34,894,947 (GRCm38)		probably null	Het
Nat10	A	T	2: 103,730,267 (GRCm38)	V731D	probably damaging	Het
Ncapd2	A	G	6: 125,168,700 (GRCm38)	V1328A	probably benign	Het
Nfu1	C	A	6: 87,025,559 (GRCm38)	Q207K	probably damaging	Het
Nipsnap2	T	A	5: 129,739,769 (GRCm38)		probably null	Het
Npc1	T	C	18: 12,191,789 (GRCm38)	K1216E	possibly damaging	Het
Or2r3	C	A	6: 42,471,972 (GRCm38)	V69L	probably benign	Het
Phf3	T	C	1: 30,804,683 (GRCm38)	I1732V	probably damaging	Het
Rasgrp4	G	T	7: 29,137,717 (GRCm38)	R67L	probably benign	Het
Rubcn	T	C	16: 32,849,721 (GRCm38)	D88G	probably damaging	Het
Slc30a2	T	C	4: 134,345,978 (GRCm38)	I112T	probably damaging	Het
Slc35d2	A	G	13: 64,120,605 (GRCm38)	I78T	probably damaging	Het
Slc9a4	T	A	1: 40,619,117 (GRCm38)	M600K	probably damaging	Het
Slfn8	A	G	11: 83,016,736 (GRCm38)	I327T	probably benign	Het
Tas2r118	A	G	6: 23,969,471 (GRCm38)	I197T	probably benign	Het
Thsd7b	T	C	1: 129,678,084 (GRCm38)	S521P	probably benign	Het
Trim36	A	G	18: 46,169,340 (GRCm38)	L535P	probably damaging	Het
Trim63	A	T	4: 134,316,531 (GRCm38)	I102F	probably damaging	Het
Wdr47	G	A	3: 108,643,085 (GRCm38)	V809M	probably damaging	Het

Other mutations in Tex15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00639:Tex15	APN	8	33,575,311 (GRCm38)	missense	probably benign	0.18
IGL00705:Tex15	APN	8	33,581,592 (GRCm38)	missense	probably damaging	1.00
IGL00820:Tex15	APN	8	33,579,006 (GRCm38)	splice site	probably benign
IGL01288:Tex15	APN	8	33,571,384 (GRCm38)	missense	probably benign	0.02
IGL01328:Tex15	APN	8	33,571,396 (GRCm38)	nonsense	probably null
IGL01359:Tex15	APN	8	33,581,898 (GRCm38)	missense	probably damaging	0.99
IGL01603:Tex15	APN	8	33,573,547 (GRCm38)	missense	possibly damaging	0.93
IGL01861:Tex15	APN	8	33,570,689 (GRCm38)	missense	probably damaging	1.00
IGL02052:Tex15	APN	8	33,582,465 (GRCm38)	missense	probably benign	0.28
IGL02560:Tex15	APN	8	33,581,751 (GRCm38)	missense	probably benign	0.00
IGL02677:Tex15	APN	8	33,571,080 (GRCm38)	missense	probably benign	0.03
IGL02739:Tex15	APN	8	33,581,693 (GRCm38)	missense	possibly damaging	0.68
Big_gulp	UTSW	8	33,581,734 (GRCm38)	missense	probably damaging	1.00
P0005:Tex15	UTSW	8	33,570,868 (GRCm38)	missense	probably benign	0.00
P0037:Tex15	UTSW	8	33,581,580 (GRCm38)	missense	probably benign	0.00
PIT4377001:Tex15	UTSW	8	33,571,101 (GRCm38)	missense	probably damaging	1.00
R0056:Tex15	UTSW	8	33,582,027 (GRCm38)	missense	probably benign	0.00
R0056:Tex15	UTSW	8	33,582,027 (GRCm38)	missense	probably benign	0.00
R0058:Tex15	UTSW	8	33,581,502 (GRCm38)	splice site	probably benign
R0058:Tex15	UTSW	8	33,581,502 (GRCm38)	splice site	probably benign
R0595:Tex15	UTSW	8	33,572,617 (GRCm38)	missense	probably damaging	1.00
R0646:Tex15	UTSW	8	33,582,326 (GRCm38)	missense	possibly damaging	0.83
R0688:Tex15	UTSW	8	33,573,500 (GRCm38)	missense	probably damaging	1.00
R0842:Tex15	UTSW	8	33,571,547 (GRCm38)	missense	possibly damaging	0.95
R0987:Tex15	UTSW	8	33,576,847 (GRCm38)	missense	probably damaging	1.00
R1084:Tex15	UTSW	8	33,577,004 (GRCm38)	missense	probably benign	0.28
R1183:Tex15	UTSW	8	33,574,865 (GRCm38)	missense	probably benign	0.35
R1186:Tex15	UTSW	8	33,571,633 (GRCm38)	missense	probably benign	0.19
R1378:Tex15	UTSW	8	33,575,216 (GRCm38)	missense	probably damaging	0.99
R1500:Tex15	UTSW	8	33,575,092 (GRCm38)	missense	probably damaging	0.96
R1508:Tex15	UTSW	8	33,576,852 (GRCm38)	missense	probably damaging	1.00
R1597:Tex15	UTSW	8	33,571,483 (GRCm38)	missense	probably damaging	0.96
R1636:Tex15	UTSW	8	33,576,387 (GRCm38)	nonsense	probably null
R1639:Tex15	UTSW	8	33,570,817 (GRCm38)	missense	possibly damaging	0.94
R1809:Tex15	UTSW	8	33,574,234 (GRCm38)	missense	probably benign
R1843:Tex15	UTSW	8	33,576,654 (GRCm38)	missense	probably benign	0.27
R2029:Tex15	UTSW	8	33,571,274 (GRCm38)	missense	probably damaging	0.99
R2228:Tex15	UTSW	8	33,571,237 (GRCm38)	missense	probably benign	0.05
R2229:Tex15	UTSW	8	33,571,237 (GRCm38)	missense	probably benign	0.05
R2245:Tex15	UTSW	8	33,571,496 (GRCm38)	missense	possibly damaging	0.77
R2246:Tex15	UTSW	8	33,582,512 (GRCm38)	missense	possibly damaging	0.49
R2880:Tex15	UTSW	8	33,574,907 (GRCm38)	nonsense	probably null
R2881:Tex15	UTSW	8	33,574,907 (GRCm38)	nonsense	probably null
R2882:Tex15	UTSW	8	33,574,907 (GRCm38)	nonsense	probably null
R3001:Tex15	UTSW	8	33,574,528 (GRCm38)	missense	probably benign	0.15
R3002:Tex15	UTSW	8	33,574,528 (GRCm38)	missense	probably benign	0.15
R3020:Tex15	UTSW	8	33,576,670 (GRCm38)	missense	probably damaging	1.00
R3084:Tex15	UTSW	8	33,574,885 (GRCm38)	missense	probably benign	0.11
R3085:Tex15	UTSW	8	33,574,885 (GRCm38)	missense	probably benign	0.11
R3701:Tex15	UTSW	8	33,574,166 (GRCm38)	missense	probably benign	0.00
R3702:Tex15	UTSW	8	33,574,166 (GRCm38)	missense	probably benign	0.00
R3752:Tex15	UTSW	8	33,571,415 (GRCm38)	missense	probably benign
R4162:Tex15	UTSW	8	33,581,558 (GRCm38)	missense	probably damaging	1.00
R4231:Tex15	UTSW	8	33,572,137 (GRCm38)	missense	probably damaging	0.99
R4589:Tex15	UTSW	8	33,557,373 (GRCm38)	missense	probably damaging	1.00
R4707:Tex15	UTSW	8	33,582,497 (GRCm38)	missense	probably benign	0.00
R4773:Tex15	UTSW	8	33,582,732 (GRCm38)	missense	probably benign	0.42
R4967:Tex15	UTSW	8	33,574,470 (GRCm38)	missense	probably benign	0.34
R5063:Tex15	UTSW	8	33,582,610 (GRCm38)	missense	possibly damaging	0.59
R5121:Tex15	UTSW	8	33,571,766 (GRCm38)	missense	probably damaging	1.00
R5147:Tex15	UTSW	8	33,572,312 (GRCm38)	nonsense	probably null
R5166:Tex15	UTSW	8	33,576,392 (GRCm38)	missense	probably benign	0.07
R5173:Tex15	UTSW	8	33,571,740 (GRCm38)	missense	possibly damaging	0.73
R5439:Tex15	UTSW	8	33,574,171 (GRCm38)	missense	possibly damaging	0.93
R5537:Tex15	UTSW	8	33,571,613 (GRCm38)	missense	probably damaging	1.00
R5580:Tex15	UTSW	8	33,572,429 (GRCm38)	missense	probably damaging	1.00
R5588:Tex15	UTSW	8	33,577,187 (GRCm38)	missense	probably damaging	1.00
R5696:Tex15	UTSW	8	33,573,192 (GRCm38)	missense	probably benign	0.01
R5734:Tex15	UTSW	8	33,546,336 (GRCm38)	missense	probably benign	0.01
R5756:Tex15	UTSW	8	33,575,833 (GRCm38)	missense	probably benign	0.17
R6126:Tex15	UTSW	8	33,573,563 (GRCm38)	missense	probably benign	0.19
R6129:Tex15	UTSW	8	33,574,130 (GRCm38)	missense	possibly damaging	0.90
R6276:Tex15	UTSW	8	33,577,189 (GRCm38)	missense	possibly damaging	0.93
R6374:Tex15	UTSW	8	33,575,912 (GRCm38)	missense	probably damaging	1.00
R6430:Tex15	UTSW	8	33,571,301 (GRCm38)	missense	probably benign	0.01
R6452:Tex15	UTSW	8	33,572,816 (GRCm38)	missense	probably damaging	1.00
R6471:Tex15	UTSW	8	33,581,734 (GRCm38)	missense	probably damaging	1.00
R6700:Tex15	UTSW	8	33,574,889 (GRCm38)	missense	possibly damaging	0.93
R6918:Tex15	UTSW	8	33,573,184 (GRCm38)	missense	probably benign	0.27
R6958:Tex15	UTSW	8	33,570,871 (GRCm38)	missense	probably benign	0.01
R6970:Tex15	UTSW	8	33,557,428 (GRCm38)	missense	probably benign	0.03
R7059:Tex15	UTSW	8	33,574,730 (GRCm38)	missense	possibly damaging	0.57
R7069:Tex15	UTSW	8	33,570,720 (GRCm38)	missense	probably benign
R7072:Tex15	UTSW	8	33,575,431 (GRCm38)	missense	possibly damaging	0.85
R7212:Tex15	UTSW	8	33,572,995 (GRCm38)	missense	probably damaging	1.00
R7212:Tex15	UTSW	8	33,570,826 (GRCm38)	nonsense	probably null
R7216:Tex15	UTSW	8	33,572,986 (GRCm38)	missense	possibly damaging	0.93
R7219:Tex15	UTSW	8	33,546,240 (GRCm38)	missense	probably benign	0.40
R7313:Tex15	UTSW	8	33,574,817 (GRCm38)	missense	possibly damaging	0.82
R7315:Tex15	UTSW	8	33,581,516 (GRCm38)	missense	probably benign	0.01
R7444:Tex15	UTSW	8	33,576,562 (GRCm38)	missense	possibly damaging	0.92
R7455:Tex15	UTSW	8	33,576,997 (GRCm38)	missense	possibly damaging	0.91
R7643:Tex15	UTSW	8	33,575,120 (GRCm38)	missense	probably damaging	1.00
R7644:Tex15	UTSW	8	33,574,417 (GRCm38)	missense	probably benign	0.01
R7724:Tex15	UTSW	8	33,546,263 (GRCm38)	missense	possibly damaging	0.60
R7779:Tex15	UTSW	8	33,575,281 (GRCm38)	missense	probably damaging	1.00
R7798:Tex15	UTSW	8	33,581,847 (GRCm38)	missense	possibly damaging	0.69
R7816:Tex15	UTSW	8	33,581,655 (GRCm38)	missense	probably benign	0.14
R7820:Tex15	UTSW	8	33,575,062 (GRCm38)	missense	probably damaging	0.98
R8041:Tex15	UTSW	8	33,575,846 (GRCm38)	missense	probably damaging	1.00
R8150:Tex15	UTSW	8	33,573,506 (GRCm38)	missense	probably benign	0.06
R8152:Tex15	UTSW	8	33,572,893 (GRCm38)	missense	possibly damaging	0.82
R8237:Tex15	UTSW	8	33,577,399 (GRCm38)	missense	possibly damaging	0.72
R8250:Tex15	UTSW	8	33,565,205 (GRCm38)	missense	probably null	0.27
R8264:Tex15	UTSW	8	33,582,362 (GRCm38)	missense	probably benign	0.18
R8279:Tex15	UTSW	8	33,571,737 (GRCm38)	missense	probably damaging	0.96
R8353:Tex15	UTSW	8	33,576,871 (GRCm38)	nonsense	probably null
R8388:Tex15	UTSW	8	33,575,209 (GRCm38)	missense	probably benign	0.00
R8432:Tex15	UTSW	8	33,576,544 (GRCm38)	missense	probably damaging	0.99
R8453:Tex15	UTSW	8	33,576,871 (GRCm38)	nonsense	probably null
R8489:Tex15	UTSW	8	33,577,546 (GRCm38)	missense	probably benign	0.02
R8670:Tex15	UTSW	8	33,574,718 (GRCm38)	missense	probably benign	0.19
R8703:Tex15	UTSW	8	33,572,696 (GRCm38)	missense	probably benign	0.00
R8871:Tex15	UTSW	8	33,576,964 (GRCm38)	missense	possibly damaging	0.62
R8945:Tex15	UTSW	8	33,574,696 (GRCm38)	missense	probably benign	0.00
R9104:Tex15	UTSW	8	33,570,922 (GRCm38)	missense	possibly damaging	0.86
R9132:Tex15	UTSW	8	33,577,526 (GRCm38)	missense	possibly damaging	0.84
R9207:Tex15	UTSW	8	33,575,756 (GRCm38)	missense	probably damaging	1.00
R9210:Tex15	UTSW	8	33,574,291 (GRCm38)	missense	possibly damaging	0.91
R9330:Tex15	UTSW	8	33,575,115 (GRCm38)	missense	probably benign	0.01
R9354:Tex15	UTSW	8	33,573,316 (GRCm38)	missense	possibly damaging	0.86
R9365:Tex15	UTSW	8	33,574,536 (GRCm38)	missense	possibly damaging	0.56
R9440:Tex15	UTSW	8	33,582,245 (GRCm38)	missense	possibly damaging	0.90
R9534:Tex15	UTSW	8	33,570,971 (GRCm38)	missense	probably benign	0.45
R9570:Tex15	UTSW	8	33,577,281 (GRCm38)	missense	probably damaging	0.96
R9574:Tex15	UTSW	8	33,574,481 (GRCm38)	missense	probably benign	0.09
R9618:Tex15	UTSW	8	33,572,369 (GRCm38)	missense	probably benign	0.35
R9655:Tex15	UTSW	8	33,576,756 (GRCm38)	nonsense	probably null
R9786:Tex15	UTSW	8	33,572,429 (GRCm38)	missense	probably damaging	1.00
R9798:Tex15	UTSW	8	33,572,693 (GRCm38)	missense	probably damaging	0.98
RF005:Tex15	UTSW	8	33,576,677 (GRCm38)	missense	probably benign	0.05
X0020:Tex15	UTSW	8	33,576,579 (GRCm38)	missense	probably benign	0.03
X0065:Tex15	UTSW	8	33,575,517 (GRCm38)	nonsense	probably null
Z1088:Tex15	UTSW	8	33,571,315 (GRCm38)	missense	possibly damaging	0.89
Z1088:Tex15	UTSW	8	33,574,870 (GRCm38)	missense	probably benign
Z1088:Tex15	UTSW	8	33,571,810 (GRCm38)	missense	possibly damaging	0.68
Z1176:Tex15	UTSW	8	33,574,726 (GRCm38)	missense	possibly damaging	0.84

Predicted Primers

PCR Primer
(F):5'- GAGATGGGTCCTTTCAGCTC -3'
(R):5'- GTCAGCTTTATTTGTGAATCACCAC -3'

Sequencing Primer
(F):5'- AGATGGGTCCTTTCAGCTCAGATAC -3'
(R):5'- CAACCCAAACTCTTATCCAATGG -3'

Posted On

2016-12-20