Mutagenetix > Incidental Mutation

Incidental Mutation 'R5826:Vmn2r73'

450172

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r73

Ensembl Gene

ENSMUSG00000070458

Gene Name

vomeronasal 2, receptor 73

Synonyms

EG620928

MMRRC Submission

043217-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.172) question?

Stock #

R5826 (G1)

Quality Score

220

Status

Not validated

Chromosome

Chromosomal Location

85506755-85525146 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 85524956 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 64 (D64G)

Ref Sequence

ENSEMBL: ENSMUSP00000076687 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000077478]

AlphaFold

D3Z7M3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000077478
AA Change: D64G

PolyPhen 2 Score 0.770 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000076687
Gene: ENSMUSG00000070458
AA Change: D64G

Domain	Start	End	E-Value	Type
signal peptide	1	18	N/A	INTRINSIC
Pfam:ANF_receptor	77	465	1.4e-30	PFAM
Pfam:NCD3G	508	560	5.9e-21	PFAM
Pfam:7tm_3	590	827	4.4e-51	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 95.7%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 42 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca13	A	G	11: 9,632,056 (GRCm39)	H4992R	probably damaging	Het
Acyp2	C	T	11: 30,456,354 (GRCm39)	E98K	possibly damaging	Het
Akr1c20	T	C	13: 4,560,222 (GRCm39)	E152G	probably damaging	Het
Ano4	T	A	10: 88,788,189 (GRCm39)	D877V	probably damaging	Het
Asb18	A	C	1: 89,942,260 (GRCm39)	S14A	probably damaging	Het
Atrnl1	T	A	19: 57,618,724 (GRCm39)	Y147*	probably null	Het
Cbfa2t2	A	G	2: 154,342,375 (GRCm39)	I30M	possibly damaging	Het
Cpd	A	T	11: 76,675,242 (GRCm39)	L1293*	probably null	Het
Csmd2	T	C	4: 128,412,992 (GRCm39)		probably null	Het
Cst9	G	A	2: 148,680,393 (GRCm39)	V120I	possibly damaging	Het
Ddah2	A	G	17: 35,279,664 (GRCm39)	D128G	probably damaging	Het
Defb11	T	C	8: 22,395,510 (GRCm39)	I56V	probably benign	Het
Dnah17	A	T	11: 117,925,193 (GRCm39)	L3880Q	probably damaging	Het
Dnah2	C	T	11: 69,349,746 (GRCm39)	R2399Q	probably benign	Het
Dop1a	A	G	9: 86,389,623 (GRCm39)	T508A	possibly damaging	Het
Ephb2	T	A	4: 136,388,048 (GRCm39)	H685L	probably damaging	Het
Glrb	T	C	3: 80,752,449 (GRCm39)	Y387C	probably damaging	Het
Gucy2e	A	G	11: 69,126,859 (GRCm39)	S205P	possibly damaging	Het
Has2	T	A	15: 56,531,498 (GRCm39)	I406F	probably damaging	Het
Hcrtr2	A	C	9: 76,230,569 (GRCm39)	V73G	probably benign	Het
Hsd17b4	A	T	18: 50,316,239 (GRCm39)	Q622L	probably benign	Het
Nlrp1b	A	T	11: 71,072,022 (GRCm39)	M607K	probably benign	Het
Nol6	T	A	4: 41,122,158 (GRCm39)	D184V	probably benign	Het
Noxa1	T	A	2: 24,976,253 (GRCm39)	Q345L	probably damaging	Het
Nudt6	T	C	3: 37,473,617 (GRCm39)	T35A	probably benign	Het
Phf8-ps	C	A	17: 33,284,288 (GRCm39)	R838I	possibly damaging	Het
Plcg2	T	C	8: 118,337,583 (GRCm39)	V985A	probably benign	Het
Plxnc1	C	T	10: 94,635,335 (GRCm39)		probably null	Het
Prkdc	G	A	16: 15,551,962 (GRCm39)	R2056H	probably benign	Het
Ptpn4	A	T	1: 119,612,246 (GRCm39)	I49N	probably benign	Het
Ralgapa1	T	G	12: 55,723,898 (GRCm39)	S1543R	probably damaging	Het
Rnf135	A	T	11: 80,089,912 (GRCm39)	N416I	probably damaging	Het
Scn5a	A	G	9: 119,350,399 (GRCm39)	L825P	probably damaging	Het
Septin11	A	T	5: 93,287,309 (GRCm39)	N8I	possibly damaging	Het
Slc13a3	T	C	2: 165,250,876 (GRCm39)	I456V	probably benign	Het
Slc16a3	A	G	11: 120,847,756 (GRCm39)	T315A	probably benign	Het
Sun1	T	G	5: 139,231,171 (GRCm39)	F657C	probably damaging	Het
Tmco3	T	C	8: 13,360,314 (GRCm39)	S34P	probably damaging	Het
Tnrc18	G	A	5: 142,759,502 (GRCm39)	P778L	unknown	Het
Ubxn4	A	C	1: 128,194,058 (GRCm39)	K284T	possibly damaging	Het
Usp37	A	T	1: 74,509,785 (GRCm39)	N461K	probably damaging	Het
Vmn2r106	A	T	17: 20,499,133 (GRCm39)	F259L	probably benign	Het

Other mutations in Vmn2r73

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00158:Vmn2r73	APN	7	85,506,795 (GRCm39)	missense
IGL01151:Vmn2r73	APN	7	85,507,086 (GRCm39)	missense	probably damaging	0.99
IGL01408:Vmn2r73	APN	7	85,521,455 (GRCm39)	missense	probably benign	0.45
IGL01505:Vmn2r73	APN	7	85,507,267 (GRCm39)	nonsense	probably null
IGL01731:Vmn2r73	APN	7	85,506,757 (GRCm39)	makesense	probably null
IGL01818:Vmn2r73	APN	7	85,519,109 (GRCm39)	splice site	probably benign
IGL02236:Vmn2r73	APN	7	85,521,902 (GRCm39)	missense	probably benign	0.00
IGL02265:Vmn2r73	APN	7	85,520,847 (GRCm39)	missense	probably benign	0.02
IGL02266:Vmn2r73	APN	7	85,525,007 (GRCm39)	missense	possibly damaging	0.52
IGL02276:Vmn2r73	APN	7	85,518,980 (GRCm39)	critical splice donor site	probably null
IGL02284:Vmn2r73	APN	7	85,507,123 (GRCm39)	missense	possibly damaging	0.92
IGL02380:Vmn2r73	APN	7	85,507,383 (GRCm39)	missense	probably benign	0.00
IGL02466:Vmn2r73	APN	7	85,522,084 (GRCm39)	missense	probably damaging	1.00
IGL03172:Vmn2r73	APN	7	85,507,495 (GRCm39)	missense	probably benign	0.01
IGL03179:Vmn2r73	APN	7	85,519,468 (GRCm39)	missense	probably benign	0.04
G1Funyon:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R0077:Vmn2r73	UTSW	7	85,525,075 (GRCm39)	missense	probably benign	0.00
R0311:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R0413:Vmn2r73	UTSW	7	85,521,087 (GRCm39)	missense	possibly damaging	0.68
R0494:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R1523:Vmn2r73	UTSW	7	85,519,486 (GRCm39)	missense	probably benign	0.02
R1618:Vmn2r73	UTSW	7	85,525,120 (GRCm39)	nonsense	probably null
R1667:Vmn2r73	UTSW	7	85,506,889 (GRCm39)	missense	probably benign	0.00
R1728:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1729:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R1747:Vmn2r73	UTSW	7	85,507,375 (GRCm39)	missense	probably damaging	1.00
R1784:Vmn2r73	UTSW	7	85,507,086 (GRCm39)	missense	probably damaging	1.00
R2152:Vmn2r73	UTSW	7	85,506,936 (GRCm39)	missense	probably benign	0.03
R2395:Vmn2r73	UTSW	7	85,506,975 (GRCm39)	missense	probably damaging	1.00
R2415:Vmn2r73	UTSW	7	85,521,431 (GRCm39)	missense	probably damaging	1.00
R2926:Vmn2r73	UTSW	7	85,520,871 (GRCm39)	missense	probably benign	0.17
R3719:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R3766:Vmn2r73	UTSW	7	85,521,198 (GRCm39)	missense	probably benign
R3838:Vmn2r73	UTSW	7	85,507,258 (GRCm39)	missense	probably benign	0.00
R3890:Vmn2r73	UTSW	7	85,507,144 (GRCm39)	missense	probably benign
R4030:Vmn2r73	UTSW	7	85,521,044 (GRCm39)	missense	possibly damaging	0.92
R4247:Vmn2r73	UTSW	7	85,521,883 (GRCm39)	missense	probably damaging	0.99
R4409:Vmn2r73	UTSW	7	85,520,768 (GRCm39)	missense	probably damaging	0.97
R4427:Vmn2r73	UTSW	7	85,506,981 (GRCm39)	missense	probably damaging	1.00
R4430:Vmn2r73	UTSW	7	85,519,449 (GRCm39)	missense	probably benign
R4552:Vmn2r73	UTSW	7	85,525,055 (GRCm39)	missense	probably benign	0.12
R4779:Vmn2r73	UTSW	7	85,520,923 (GRCm39)	nonsense	probably null
R4906:Vmn2r73	UTSW	7	85,506,829 (GRCm39)	missense	probably damaging	0.99
R4942:Vmn2r73	UTSW	7	85,519,582 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r73	UTSW	7	85,507,155 (GRCm39)	missense	probably damaging	0.98
R5344:Vmn2r73	UTSW	7	85,525,046 (GRCm39)	missense	probably benign	0.00
R5478:Vmn2r73	UTSW	7	85,518,996 (GRCm39)	missense	probably damaging	1.00
R5527:Vmn2r73	UTSW	7	85,507,575 (GRCm39)	missense	possibly damaging	0.65
R5691:Vmn2r73	UTSW	7	85,507,299 (GRCm39)	missense	probably damaging	1.00
R6018:Vmn2r73	UTSW	7	85,521,875 (GRCm39)	missense	possibly damaging	0.48
R6082:Vmn2r73	UTSW	7	85,507,429 (GRCm39)	missense	probably benign	0.00
R6111:Vmn2r73	UTSW	7	85,520,997 (GRCm39)	missense	probably benign
R6233:Vmn2r73	UTSW	7	85,519,099 (GRCm39)	missense	probably benign	0.00
R6278:Vmn2r73	UTSW	7	85,522,140 (GRCm39)	missense	probably benign
R6283:Vmn2r73	UTSW	7	85,521,049 (GRCm39)	missense	probably benign	0.00
R6307:Vmn2r73	UTSW	7	85,506,828 (GRCm39)	missense	probably damaging	1.00
R6413:Vmn2r73	UTSW	7	85,519,544 (GRCm39)	nonsense	probably null
R6782:Vmn2r73	UTSW	7	85,519,563 (GRCm39)	missense	probably benign	0.00
R6884:Vmn2r73	UTSW	7	85,507,213 (GRCm39)	missense	probably benign	0.05
R6921:Vmn2r73	UTSW	7	85,507,446 (GRCm39)	missense	probably benign	0.27
R7169:Vmn2r73	UTSW	7	85,507,663 (GRCm39)	nonsense	probably null
R7206:Vmn2r73	UTSW	7	85,522,075 (GRCm39)	missense	probably benign	0.03
R7311:Vmn2r73	UTSW	7	85,521,192 (GRCm39)	missense	possibly damaging	0.80
R7650:Vmn2r73	UTSW	7	85,521,147 (GRCm39)	missense	probably benign
R7811:Vmn2r73	UTSW	7	85,524,956 (GRCm39)	missense	possibly damaging	0.77
R8263:Vmn2r73	UTSW	7	85,507,619 (GRCm39)	missense	probably benign
R8301:Vmn2r73	UTSW	7	85,507,510 (GRCm39)	missense	probably benign	0.00
R8341:Vmn2r73	UTSW	7	85,507,128 (GRCm39)	missense	probably benign	0.01
R8500:Vmn2r73	UTSW	7	85,519,638 (GRCm39)	missense	probably benign	0.01
R8673:Vmn2r73	UTSW	7	85,521,902 (GRCm39)	missense	probably benign	0.01
R8719:Vmn2r73	UTSW	7	85,521,095 (GRCm39)	missense	probably damaging	0.96
R8783:Vmn2r73	UTSW	7	85,507,668 (GRCm39)	missense	probably damaging	0.96
R8828:Vmn2r73	UTSW	7	85,521,179 (GRCm39)	missense	probably damaging	0.98
R8860:Vmn2r73	UTSW	7	85,522,149 (GRCm39)	splice site	probably benign
R9002:Vmn2r73	UTSW	7	85,507,284 (GRCm39)	missense	probably benign	0.32
R9008:Vmn2r73	UTSW	7	85,521,904 (GRCm39)	missense	probably damaging	0.96
R9159:Vmn2r73	UTSW	7	85,521,931 (GRCm39)	missense	possibly damaging	0.90
R9180:Vmn2r73	UTSW	7	85,507,123 (GRCm39)	missense	probably benign	0.07
R9198:Vmn2r73	UTSW	7	85,518,987 (GRCm39)	missense	probably benign	0.01
R9329:Vmn2r73	UTSW	7	85,519,073 (GRCm39)	missense	probably benign
R9448:Vmn2r73	UTSW	7	85,522,027 (GRCm39)	missense	probably benign	0.00
R9463:Vmn2r73	UTSW	7	85,506,795 (GRCm39)	missense
R9589:Vmn2r73	UTSW	7	85,519,659 (GRCm39)	missense	probably benign	0.03
X0023:Vmn2r73	UTSW	7	85,507,664 (GRCm39)	missense	probably benign	0.31
Z1176:Vmn2r73	UTSW	7	85,521,176 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r73	UTSW	7	85,521,480 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGGTCATTCATTCAGCATTTACC -3'
(R):5'- CAGGCAGATATCTTGTGGACAGG -3'

Sequencing Primer
(F):5'- CAGCATTTACCTAGAAATTGAAACTC -3'
(R):5'- TGTTCTCTTTGATCTTCATTTTCTGG -3'

Posted On

2016-12-20