Mutagenetix > Incidental Mutation

Incidental Mutation 'R5828:Cacna1g'

450282

Institutional Source

Beutler Lab

Gene Symbol

Cacna1g

Ensembl Gene

ENSMUSG00000020866

Gene Name

calcium channel, voltage-dependent, T type, alpha 1G subunit

Synonyms

a1G, Cav3.1d

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.450) question?

Stock #

R5828 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

94299217-94365024 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to G at 94347980 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Arginine at position 703 (S703R)

Ref Sequence

ENSEMBL: ENSMUSP00000103414 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021234] [ENSMUST00000100561] [ENSMUST00000103166] [ENSMUST00000107785] [ENSMUST00000107786] [ENSMUST00000107788] [ENSMUST00000107789] [ENSMUST00000107790] [ENSMUST00000107791] [ENSMUST00000107792] [ENSMUST00000107793]

AlphaFold

Q5SUF7

Predicted Effect

probably benign
Transcript: ENSMUST00000021234
AA Change: S703R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000021234
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	9.2e-66	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	7.9e-46	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	3e-55	PFAM
coiled coil region	1519	1559	N/A	INTRINSIC
low complexity region	1562	1573	N/A	INTRINSIC
Pfam:Ion_trans	1630	1835	3e-49	PFAM
Pfam:PKD_channel	1688	1842	7.8e-11	PFAM
low complexity region	2180	2211	N/A	INTRINSIC
low complexity region	2230	2246	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100561
AA Change: S703R

PolyPhen 2 Score 0.369 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000098127
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6.1e-55	PFAM
coiled coil region	1542	1582	N/A	INTRINSIC
low complexity region	1585	1596	N/A	INTRINSIC
Pfam:Ion_trans	1653	1858	6.2e-49	PFAM
Pfam:PKD_channel	1711	1865	1.4e-10	PFAM
low complexity region	2203	2234	N/A	INTRINSIC
low complexity region	2253	2269	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000103166
AA Change: S703R

PolyPhen 2 Score 0.390 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000099455
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6.1e-55	PFAM
SCOP:d1g2qa_	1545	1589	6e-3	SMART
Pfam:Ion_trans	1646	1851	6.2e-49	PFAM
Pfam:PKD_channel	1704	1858	1.4e-10	PFAM
low complexity region	2196	2227	N/A	INTRINSIC
low complexity region	2246	2262	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107785
AA Change: S703R

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000103414
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.5e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	5.7e-55	PFAM
coiled coil region	1519	1558	N/A	INTRINSIC
Pfam:Ion_trans	1612	1817	5.8e-49	PFAM
Pfam:PKD_channel	1670	1824	1.4e-10	PFAM
low complexity region	2027	2042	N/A	INTRINSIC
low complexity region	2084	2115	N/A	INTRINSIC
low complexity region	2134	2150	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107786
AA Change: S686R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000103415
Gene: ENSMUSG00000020866
AA Change: S686R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	383	4e-60	PFAM
low complexity region	466	490	N/A	INTRINSIC
low complexity region	518	530	N/A	INTRINSIC
Pfam:Ion_trans	760	946	1.6e-45	PFAM
low complexity region	1023	1034	N/A	INTRINSIC
low complexity region	1046	1073	N/A	INTRINSIC
low complexity region	1094	1110	N/A	INTRINSIC
low complexity region	1176	1187	N/A	INTRINSIC
Pfam:Ion_trans	1273	1497	5.9e-55	PFAM
SCOP:d1g2qa_	1505	1549	6e-3	SMART
Pfam:Ion_trans	1606	1811	6e-49	PFAM
Pfam:PKD_channel	1664	1818	1.4e-10	PFAM
low complexity region	2156	2187	N/A	INTRINSIC
low complexity region	2206	2222	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000107788
AA Change: S703R

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000103417
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6e-55	PFAM
coiled coil region	1542	1574	N/A	INTRINSIC
Pfam:Ion_trans	1628	1833	6.1e-49	PFAM
Pfam:PKD_channel	1686	1840	1.4e-10	PFAM
low complexity region	2178	2209	N/A	INTRINSIC
low complexity region	2228	2244	N/A	INTRINSIC

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107789
AA Change: S703R

PolyPhen 2 Score 0.756 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000103418
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	80	406	1.6e-76	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	742	972	3.4e-56	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1276	1549	1.5e-61	PFAM
low complexity region	1578	1589	N/A	INTRINSIC
Pfam:Ion_trans	1611	1863	2.1e-56	PFAM
Pfam:PKD_channel	1703	1858	3.4e-9	PFAM
low complexity region	2289	2320	N/A	INTRINSIC
low complexity region	2339	2355	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152811

Predicted Effect

probably benign
Transcript: ENSMUST00000107790
AA Change: S703R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000103419
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	6e-55	PFAM
SCOP:d1g2qa_	1522	1566	6e-3	SMART
Pfam:Ion_trans	1623	1828	6.1e-49	PFAM
Pfam:PKD_channel	1681	1835	1.4e-10	PFAM
low complexity region	2173	2204	N/A	INTRINSIC
low complexity region	2223	2239	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107791
AA Change: S703R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000103420
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	6e-55	PFAM
coiled coil region	1519	1558	N/A	INTRINSIC
Pfam:Ion_trans	1612	1817	6e-49	PFAM
Pfam:PKD_channel	1670	1824	1.4e-10	PFAM
low complexity region	2162	2193	N/A	INTRINSIC
low complexity region	2212	2228	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107792
AA Change: S703R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000103421
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.8e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1040	1051	N/A	INTRINSIC
low complexity region	1063	1090	N/A	INTRINSIC
low complexity region	1111	1127	N/A	INTRINSIC
low complexity region	1193	1204	N/A	INTRINSIC
Pfam:Ion_trans	1290	1514	5.9e-55	PFAM
coiled coil region	1519	1551	N/A	INTRINSIC
Pfam:Ion_trans	1605	1810	6e-49	PFAM
Pfam:PKD_channel	1663	1817	1.4e-10	PFAM
low complexity region	2155	2186	N/A	INTRINSIC
low complexity region	2205	2221	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000107793
AA Change: S703R

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000103422
Gene: ENSMUSG00000020866
AA Change: S703R

Domain	Start	End	E-Value	Type
low complexity region	2	12	N/A	INTRINSIC
Pfam:Ion_trans	119	394	1.9e-65	PFAM
low complexity region	483	507	N/A	INTRINSIC
low complexity region	535	547	N/A	INTRINSIC
Pfam:Ion_trans	777	963	1.6e-45	PFAM
low complexity region	1063	1074	N/A	INTRINSIC
low complexity region	1086	1113	N/A	INTRINSIC
low complexity region	1134	1150	N/A	INTRINSIC
low complexity region	1216	1227	N/A	INTRINSIC
Pfam:Ion_trans	1313	1537	6e-55	PFAM
coiled coil region	1542	1581	N/A	INTRINSIC
Pfam:Ion_trans	1635	1840	6.1e-49	PFAM
Pfam:PKD_channel	1693	1847	1.4e-10	PFAM
low complexity region	2185	2216	N/A	INTRINSIC
low complexity region	2235	2251	N/A	INTRINSIC

Meta Mutation Damage Score

0.0726

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 95.8%

Validation Efficiency

100% (63/63)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Voltage-sensitive calcium channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division, and cell death. This gene encodes a T-type, low-voltage activated calcium channel. The T-type channels generate currents that are both transient, owing to fast inactivation, and tiny, owing to small conductance. T-type channels are thought to be involved in pacemaker activity, low-threshold calcium spikes, neuronal oscillations and resonance, and rebound burst firing. Many alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Homozygote null mice display disrupted sleeping patterns, altered amounts of activity, abnormal action potentials in the brain, prolonged electrical conductance in the heart, and resistance to diet-induced obesity. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933409G03Rik	A	G	2: 68,432,144 (GRCm39)	D95G	unknown	Het
Arl10	T	A	13: 54,726,768 (GRCm39)	V182E	probably damaging	Het
Ces2a	C	A	8: 105,465,956 (GRCm39)	T363N	probably benign	Het
Col14a1	G	A	15: 55,300,372 (GRCm39)	V967I	unknown	Het
Col22a1	A	T	15: 71,881,340 (GRCm39)	F4I	probably benign	Het
D630045J12Rik	A	G	6: 38,173,302 (GRCm39)	W289R	possibly damaging	Het
Disc1	A	G	8: 125,977,763 (GRCm39)	Q793R	probably damaging	Het
Dnhd1	C	T	7: 105,369,388 (GRCm39)	T4337I	probably benign	Het
Eif2b5	T	A	16: 20,321,536 (GRCm39)	V363D	possibly damaging	Het
Emsy	T	C	7: 98,242,699 (GRCm39)	T1147A	probably benign	Het
Epg5	T	A	18: 78,064,066 (GRCm39)	Y2048N	probably damaging	Het
Fchsd1	C	T	18: 38,092,926 (GRCm39)		probably benign	Het
Foxred1	A	G	9: 35,121,492 (GRCm39)		probably benign	Het
Gpaa1	T	C	15: 76,216,471 (GRCm39)		probably benign	Het
Gria4	A	C	9: 4,432,832 (GRCm39)	L784V	probably damaging	Het
Homer3	A	G	8: 70,738,956 (GRCm39)	Y105C	probably benign	Het
Hpgd	T	C	8: 56,772,106 (GRCm39)	S193P	probably benign	Het
Irf1	T	A	11: 53,666,762 (GRCm39)	W247R	probably benign	Het
Lpgat1	A	G	1: 191,508,494 (GRCm39)	Q344R	possibly damaging	Het
Luzp1	T	C	4: 136,267,993 (GRCm39)	V72A	probably damaging	Het
Malrd1	G	A	2: 15,531,464 (GRCm39)	V8M	probably benign	Het
Msmo1	T	C	8: 65,172,144 (GRCm39)	H253R	probably damaging	Het
Nom1	A	G	5: 29,640,124 (GRCm39)	K150R	possibly damaging	Het
Onecut1	A	G	9: 74,770,042 (GRCm39)	E155G	probably benign	Het
Or12k7	A	G	2: 36,959,001 (GRCm39)	H228R	probably benign	Het
Or14c46	T	A	7: 85,918,728 (GRCm39)	M90L	probably benign	Het
Or5p59	T	C	7: 107,703,005 (GRCm39)	V163A	possibly damaging	Het
Osbpl10	G	A	9: 114,890,944 (GRCm39)	V111M	probably damaging	Het
Pcdhgb6	T	C	18: 37,877,457 (GRCm39)	S722P	probably benign	Het
Plin4	T	A	17: 56,414,064 (GRCm39)	D187V	probably damaging	Het
Pmf1	T	C	3: 88,303,294 (GRCm39)	E89G	possibly damaging	Het
Polr1d	A	T	5: 147,014,408 (GRCm39)		probably benign	Het
Ppp1r9a	T	C	6: 5,158,200 (GRCm39)	Y1006H	probably damaging	Het
Ppp2r5c	A	G	12: 110,537,134 (GRCm39)	K420E	probably benign	Het
Prdx1	T	C	4: 116,551,006 (GRCm39)	L159P	probably damaging	Het
Psmd6	G	T	14: 14,119,990 (GRCm38)	D39E	probably benign	Het
Rffl	T	C	11: 82,709,244 (GRCm39)	K60E	probably damaging	Het
Rspo2	A	T	15: 42,939,283 (GRCm39)	L169Q	probably damaging	Het
Sbf1	T	A	15: 89,172,837 (GRCm39)	D1892V	probably damaging	Het
Sbno2	G	A	10: 79,902,424 (GRCm39)	S475F	possibly damaging	Het
Serpina3i	T	A	12: 104,231,474 (GRCm39)	V37E	probably benign	Het
Siglecf	G	A	7: 43,001,137 (GRCm39)	C35Y	probably damaging	Het
Slc22a27	G	T	19: 7,903,767 (GRCm39)	D123E	probably damaging	Het
Smim23	T	A	11: 32,770,592 (GRCm39)	K105*	probably null	Het
Soat1	C	T	1: 156,265,318 (GRCm39)	S348N	probably benign	Het
Sp2	C	T	11: 96,851,811 (GRCm39)		probably benign	Het
St14	T	A	9: 31,002,803 (GRCm39)	H700L	probably damaging	Het
St3gal1	T	A	15: 66,985,634 (GRCm39)	K7*	probably null	Het
Tgm1	T	C	14: 55,943,011 (GRCm39)	K610E	probably benign	Het
Tob1	T	A	11: 94,104,583 (GRCm39)	Y40N	probably damaging	Het
Tob1	T	A	11: 94,104,585 (GRCm39)	Y40*	probably null	Het
Trpc4ap	A	G	2: 155,477,130 (GRCm39)		probably benign	Het
Ttc9	C	T	12: 81,678,450 (GRCm39)	P91L	probably benign	Het
Zbtb18	T	C	1: 177,275,446 (GRCm39)	S269P	probably damaging	Het
Zfp703	C	T	8: 27,469,233 (GRCm39)	P299L	probably damaging	Het
Zfyve16	A	T	13: 92,650,410 (GRCm39)	V858E	probably damaging	Het

Other mutations in Cacna1g

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01300:Cacna1g	APN	11	94,324,738 (GRCm39)	missense	probably benign	0.16
IGL01382:Cacna1g	APN	11	94,356,684 (GRCm39)	missense	probably damaging	1.00
IGL01694:Cacna1g	APN	11	94,319,938 (GRCm39)	missense	probably damaging	1.00
IGL01866:Cacna1g	APN	11	94,347,937 (GRCm39)	missense	probably damaging	0.99
IGL02341:Cacna1g	APN	11	94,352,978 (GRCm39)	missense	probably damaging	1.00
IGL02506:Cacna1g	APN	11	94,319,955 (GRCm39)	missense	probably damaging	0.99
IGL02706:Cacna1g	APN	11	94,347,818 (GRCm39)	missense	probably damaging	1.00
IGL02879:Cacna1g	APN	11	94,300,431 (GRCm39)	missense	probably benign	0.12
IGL03366:Cacna1g	APN	11	94,347,977 (GRCm39)	missense	probably damaging	1.00
ANU23:Cacna1g	UTSW	11	94,357,054 (GRCm39)	critical splice acceptor site	probably benign
IGL03134:Cacna1g	UTSW	11	94,350,651 (GRCm39)	missense	probably damaging	1.00
R0092:Cacna1g	UTSW	11	94,348,090 (GRCm39)	missense	probably damaging	1.00
R0123:Cacna1g	UTSW	11	94,300,302 (GRCm39)	missense	probably damaging	1.00
R0178:Cacna1g	UTSW	11	94,354,309 (GRCm39)	missense	probably damaging	1.00
R0375:Cacna1g	UTSW	11	94,301,880 (GRCm39)	missense	possibly damaging	0.79
R0389:Cacna1g	UTSW	11	94,350,523 (GRCm39)	missense	probably damaging	1.00
R0433:Cacna1g	UTSW	11	94,350,033 (GRCm39)	missense	probably benign	0.16
R0458:Cacna1g	UTSW	11	94,300,266 (GRCm39)	missense	probably damaging	0.96
R0498:Cacna1g	UTSW	11	94,350,685 (GRCm39)	missense	probably damaging	1.00
R0629:Cacna1g	UTSW	11	94,300,369 (GRCm39)	missense	possibly damaging	0.93
R0800:Cacna1g	UTSW	11	94,317,265 (GRCm39)	missense	probably damaging	1.00
R1341:Cacna1g	UTSW	11	94,324,582 (GRCm39)	missense	probably damaging	0.99
R1457:Cacna1g	UTSW	11	94,350,381 (GRCm39)	missense	possibly damaging	0.76
R1523:Cacna1g	UTSW	11	94,333,555 (GRCm39)	critical splice donor site	probably null
R1532:Cacna1g	UTSW	11	94,334,157 (GRCm39)	missense	probably damaging	1.00
R1540:Cacna1g	UTSW	11	94,347,865 (GRCm39)	missense	probably damaging	1.00
R1652:Cacna1g	UTSW	11	94,318,230 (GRCm39)	missense	probably damaging	1.00
R1688:Cacna1g	UTSW	11	94,316,779 (GRCm39)	missense	possibly damaging	0.70
R1750:Cacna1g	UTSW	11	94,334,118 (GRCm39)	missense	probably damaging	1.00
R1751:Cacna1g	UTSW	11	94,350,628 (GRCm39)	missense	probably benign	0.35
R1767:Cacna1g	UTSW	11	94,350,628 (GRCm39)	missense	probably benign	0.35
R1847:Cacna1g	UTSW	11	94,357,007 (GRCm39)	missense	probably damaging	1.00
R1924:Cacna1g	UTSW	11	94,334,880 (GRCm39)	missense	possibly damaging	0.93
R1973:Cacna1g	UTSW	11	94,350,603 (GRCm39)	missense	possibly damaging	0.86
R2050:Cacna1g	UTSW	11	94,300,300 (GRCm39)	missense	probably damaging	1.00
R2261:Cacna1g	UTSW	11	94,347,961 (GRCm39)	missense	probably benign	0.42
R2273:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2274:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2275:Cacna1g	UTSW	11	94,306,762 (GRCm39)	missense	probably damaging	0.98
R2376:Cacna1g	UTSW	11	94,356,734 (GRCm39)	missense	probably damaging	0.99
R3686:Cacna1g	UTSW	11	94,349,916 (GRCm39)	splice site	probably null
R3809:Cacna1g	UTSW	11	94,306,922 (GRCm39)	missense	probably damaging	1.00
R3875:Cacna1g	UTSW	11	94,328,749 (GRCm39)	missense	probably damaging	0.98
R4133:Cacna1g	UTSW	11	94,323,370 (GRCm39)	missense	probably damaging	1.00
R4642:Cacna1g	UTSW	11	94,308,920 (GRCm39)	missense	probably damaging	1.00
R4732:Cacna1g	UTSW	11	94,334,041 (GRCm39)	missense	probably damaging	1.00
R4733:Cacna1g	UTSW	11	94,334,041 (GRCm39)	missense	probably damaging	1.00
R4773:Cacna1g	UTSW	11	94,302,298 (GRCm39)	missense	possibly damaging	0.78
R4798:Cacna1g	UTSW	11	94,324,673 (GRCm39)	missense	probably damaging	1.00
R4839:Cacna1g	UTSW	11	94,350,433 (GRCm39)	missense	probably benign	0.16
R4900:Cacna1g	UTSW	11	94,350,177 (GRCm39)	missense	possibly damaging	0.78
R4927:Cacna1g	UTSW	11	94,319,973 (GRCm39)	missense	probably damaging	1.00
R4930:Cacna1g	UTSW	11	94,334,899 (GRCm39)	missense	probably damaging	1.00
R5050:Cacna1g	UTSW	11	94,350,541 (GRCm39)	missense	probably damaging	1.00
R5117:Cacna1g	UTSW	11	94,323,329 (GRCm39)	missense	probably damaging	1.00
R5186:Cacna1g	UTSW	11	94,333,674 (GRCm39)	missense	probably damaging	1.00
R5364:Cacna1g	UTSW	11	94,307,684 (GRCm39)	missense	probably benign	0.29
R5512:Cacna1g	UTSW	11	94,334,968 (GRCm39)	missense	probably damaging	1.00
R5564:Cacna1g	UTSW	11	94,321,312 (GRCm39)	missense	probably damaging	1.00
R5603:Cacna1g	UTSW	11	94,330,578 (GRCm39)	missense	possibly damaging	0.76
R5682:Cacna1g	UTSW	11	94,349,940 (GRCm39)	missense	probably damaging	1.00
R5818:Cacna1g	UTSW	11	94,308,946 (GRCm39)	missense	probably damaging	1.00
R5882:Cacna1g	UTSW	11	94,350,645 (GRCm39)	missense	probably damaging	1.00
R5884:Cacna1g	UTSW	11	94,328,693 (GRCm39)	missense	probably damaging	1.00
R6075:Cacna1g	UTSW	11	94,307,491 (GRCm39)	missense	probably damaging	1.00
R6112:Cacna1g	UTSW	11	94,300,072 (GRCm39)	missense	probably damaging	0.98
R6122:Cacna1g	UTSW	11	94,320,997 (GRCm39)	missense	probably benign	0.11
R6145:Cacna1g	UTSW	11	94,353,087 (GRCm39)	missense	probably damaging	1.00
R6362:Cacna1g	UTSW	11	94,330,533 (GRCm39)	critical splice donor site	probably null
R6415:Cacna1g	UTSW	11	94,354,243 (GRCm39)	missense	probably damaging	1.00
R6468:Cacna1g	UTSW	11	94,330,548 (GRCm39)	missense	probably damaging	1.00
R6648:Cacna1g	UTSW	11	94,323,395 (GRCm39)	missense	probably damaging	1.00
R6746:Cacna1g	UTSW	11	94,300,253 (GRCm39)	nonsense	probably null
R6764:Cacna1g	UTSW	11	94,304,014 (GRCm39)	missense	possibly damaging	0.95
R6782:Cacna1g	UTSW	11	94,350,376 (GRCm39)	missense	probably damaging	1.00
R6888:Cacna1g	UTSW	11	94,350,033 (GRCm39)	missense	probably benign	0.16
R7148:Cacna1g	UTSW	11	94,356,756 (GRCm39)	missense	probably benign	0.32
R7181:Cacna1g	UTSW	11	94,306,691 (GRCm39)	missense	probably benign	0.21
R7183:Cacna1g	UTSW	11	94,330,563 (GRCm39)	missense	probably benign	0.04
R7193:Cacna1g	UTSW	11	94,300,057 (GRCm39)	missense	possibly damaging	0.60
R7237:Cacna1g	UTSW	11	94,328,705 (GRCm39)	missense	probably benign	0.21
R7254:Cacna1g	UTSW	11	94,323,393 (GRCm39)	nonsense	probably null
R7312:Cacna1g	UTSW	11	94,323,383 (GRCm39)	missense	probably damaging	1.00
R7451:Cacna1g	UTSW	11	94,319,901 (GRCm39)	missense	probably damaging	1.00
R7470:Cacna1g	UTSW	11	94,352,765 (GRCm39)	missense	possibly damaging	0.76
R7534:Cacna1g	UTSW	11	94,301,904 (GRCm39)	missense	probably benign	0.00
R7585:Cacna1g	UTSW	11	94,364,368 (GRCm39)	missense	probably benign	0.39
R7706:Cacna1g	UTSW	11	94,305,867 (GRCm39)	missense	probably benign	0.06
R7812:Cacna1g	UTSW	11	94,334,880 (GRCm39)	missense	probably benign	0.03
R7918:Cacna1g	UTSW	11	94,334,856 (GRCm39)	missense	probably benign	0.03
R7947:Cacna1g	UTSW	11	94,348,001 (GRCm39)	missense	probably benign	0.01
R8013:Cacna1g	UTSW	11	94,347,796 (GRCm39)	missense	probably damaging	0.99
R8016:Cacna1g	UTSW	11	94,334,007 (GRCm39)	missense	probably benign	0.36
R8029:Cacna1g	UTSW	11	94,300,564 (GRCm39)	missense	probably benign	0.01
R8098:Cacna1g	UTSW	11	94,307,338 (GRCm39)	missense	probably benign	0.20
R8264:Cacna1g	UTSW	11	94,364,392 (GRCm39)	missense	probably benign	0.21
R8478:Cacna1g	UTSW	11	94,317,278 (GRCm39)	missense	probably damaging	1.00
R8679:Cacna1g	UTSW	11	94,319,962 (GRCm39)	missense	probably damaging	1.00
R8697:Cacna1g	UTSW	11	94,307,524 (GRCm39)	missense	probably benign	0.09
R8772:Cacna1g	UTSW	11	94,356,713 (GRCm39)	missense	probably benign	0.03
R9011:Cacna1g	UTSW	11	94,306,663 (GRCm39)	missense	probably benign	0.21
R9085:Cacna1g	UTSW	11	94,334,046 (GRCm39)	missense	probably benign	0.01
R9155:Cacna1g	UTSW	11	94,350,423 (GRCm39)	missense
R9243:Cacna1g	UTSW	11	94,347,893 (GRCm39)	missense	possibly damaging	0.84
R9288:Cacna1g	UTSW	11	94,308,897 (GRCm39)	nonsense	probably null
R9408:Cacna1g	UTSW	11	94,321,050 (GRCm39)	missense	probably damaging	1.00
R9496:Cacna1g	UTSW	11	94,356,711 (GRCm39)	missense	probably benign	0.03
R9607:Cacna1g	UTSW	11	94,356,714 (GRCm39)	missense	probably benign	0.03
R9720:Cacna1g	UTSW	11	94,302,297 (GRCm39)	missense	probably benign	0.01
X0001:Cacna1g	UTSW	11	94,300,471 (GRCm39)	missense	possibly damaging	0.90
X0019:Cacna1g	UTSW	11	94,350,079 (GRCm39)	missense	probably damaging	0.97
X0065:Cacna1g	UTSW	11	94,353,251 (GRCm39)	missense	probably damaging	1.00
Z1176:Cacna1g	UTSW	11	94,328,937 (GRCm39)	missense	probably benign	0.31
Z1177:Cacna1g	UTSW	11	94,364,416 (GRCm39)	missense	probably benign	0.21
Z1177:Cacna1g	UTSW	11	94,350,422 (GRCm39)	missense	probably benign	0.04

Predicted Primers

PCR Primer
(F):5'- AAGCCATTGTCCAGAGCCAG -3'
(R):5'- TAGCTCCTGCAAAATCTCCAG -3'

Sequencing Primer
(F):5'- TACTCGATGCCCATGCTGAG -3'
(R):5'- TGCAAAATCTCCAGCCCTTG -3'

Posted On

2016-12-20