Mutagenetix > Incidental Mutation

Incidental Mutation 'R5844:Or5g25'

450574

Institutional Source

Beutler Lab

Gene Symbol

Or5g25

Ensembl Gene

ENSMUSG00000075214

Gene Name

olfactory receptor family 5 subfamily G member 25

Synonyms

MOR175-2, Olfr1002, GA_x6K02T2Q125-47127746-47126790

MMRRC Submission

044062-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.059) question?

Stock #

R5844 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

85477707-85478663 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 85478239 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 142 (V142A)

Ref Sequence

ENSEMBL: ENSMUSP00000150405 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099920] [ENSMUST00000215548]

AlphaFold

Q8VFK2

Predicted Effect

probably benign
Transcript: ENSMUST00000099920
AA Change: V142A

PolyPhen 2 Score 0.361 (Sensitivity: 0.90; Specificity: 0.89)

SMART Domains

Protein: ENSMUSP00000097504
Gene: ENSMUSG00000075214
AA Change: V142A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	8.7e-50	PFAM
Pfam:7tm_1	41	290	2.5e-17	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000215548
AA Change: V142A

PolyPhen 2 Score 0.361 (Sensitivity: 0.90; Specificity: 0.89)

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.9%
3x: 99.7%
10x: 98.5%
20x: 95.9%

Validation Efficiency

98% (55/56)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 50 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930407I10Rik	A	T	15: 81,950,065 (GRCm39)	M1321L	probably benign	Het
Adra1a	C	T	14: 66,965,183 (GRCm39)	T391I	probably benign	Het
Atf7ip	C	A	6: 136,583,812 (GRCm39)	A1281D	probably damaging	Het
BC005624	T	C	2: 30,866,023 (GRCm39)	N141S	probably benign	Het
Catsperg2	A	T	7: 29,397,257 (GRCm39)	L1082Q	possibly damaging	Het
Cavin2	C	T	1: 51,328,998 (GRCm39)	R152C	probably damaging	Het
Ccdc33	C	T	9: 57,940,489 (GRCm39)		probably benign	Het
Cfap43	T	C	19: 47,784,135 (GRCm39)	D466G	probably benign	Het
Cfap46	C	A	7: 139,230,858 (GRCm39)	M923I	probably damaging	Het
Chd1l	T	A	3: 97,479,883 (GRCm39)	K621N	probably benign	Het
Cnksr1	A	G	4: 133,955,575 (GRCm39)		probably benign	Het
Cym	T	C	3: 107,127,080 (GRCm39)	H25R	probably benign	Het
Dagla	T	A	19: 10,248,489 (GRCm39)	D57V	probably damaging	Het
Dnah3	TTCCTC	TTC	7: 119,550,244 (GRCm39)		probably benign	Het
Dse	T	A	10: 34,029,038 (GRCm39)	D684V	probably damaging	Het
Eml2	G	A	7: 18,935,088 (GRCm39)	V432I	probably damaging	Het
Fbxo10	A	T	4: 45,058,760 (GRCm39)	S326T	probably benign	Het
Galntl5	G	T	5: 25,391,091 (GRCm39)		probably benign	Het
Grm5	A	G	7: 87,453,232 (GRCm39)	R290G	possibly damaging	Het
Gtpbp3	A	G	8: 71,945,199 (GRCm39)	T425A	probably benign	Het
Hepacam2	A	G	6: 3,476,073 (GRCm39)	I284T	probably damaging	Het
Ifi205	A	C	1: 173,854,258 (GRCm39)		probably null	Het
Irs3	T	C	5: 137,642,548 (GRCm39)	T297A	probably benign	Het
Kmt2d	G	A	15: 98,749,990 (GRCm39)		probably benign	Het
Map4k4	T	A	1: 40,039,036 (GRCm39)		probably benign	Het
Mfsd6	T	C	1: 52,697,542 (GRCm39)	S782G	probably benign	Het
Mis18a	G	A	16: 90,523,969 (GRCm39)		silent	Het
Mtpn	C	T	6: 35,489,225 (GRCm39)	D100N	probably benign	Het
Myom2	G	A	8: 15,181,182 (GRCm39)		probably null	Het
Ntaq1	A	G	15: 58,017,056 (GRCm39)	N157S	probably benign	Het
Or13a1	A	T	6: 116,470,900 (GRCm39)	E110V	probably damaging	Het
Pde3b	C	T	7: 114,108,106 (GRCm39)	T568I	probably benign	Het
Pip4p1	T	C	14: 51,166,499 (GRCm39)	T160A	probably benign	Het
Pkhd1	G	T	1: 20,451,685 (GRCm39)	D2203E	probably benign	Het
Ppp1r36	A	G	12: 76,473,566 (GRCm39)	K66E	possibly damaging	Het
Rfc1	C	A	5: 65,451,130 (GRCm39)	M319I	probably benign	Het
Rnd2	C	T	11: 101,359,825 (GRCm39)	L57F	probably damaging	Het
Runx1t1	T	C	4: 13,881,068 (GRCm39)	V456A	probably damaging	Het
Rxfp2	A	G	5: 149,966,589 (GRCm39)	K109R	probably benign	Het
Sgo2a	T	G	1: 58,055,556 (GRCm39)	V580G	probably damaging	Het
Skint9	T	C	4: 112,271,080 (GRCm39)	Q110R	probably benign	Het
Slc38a9	A	G	13: 112,868,035 (GRCm39)	Y507C	probably damaging	Het
Smarca4	T	A	9: 21,589,238 (GRCm39)		probably benign	Het
Tmem88	C	G	11: 69,288,504 (GRCm39)	Q138H	probably benign	Het
Tns2	C	T	15: 102,017,369 (GRCm39)	R281C	probably damaging	Het
Tns3	A	C	11: 8,384,580 (GRCm39)	F1413V	probably damaging	Het
Trpm8	T	C	1: 88,312,433 (GRCm39)	*1105Q	probably null	Het
Zfp853	C	T	5: 143,274,424 (GRCm39)	V399M	unknown	Het
Zim1	T	C	7: 6,681,115 (GRCm39)	R183G	probably benign	Het
Zmiz1	T	C	14: 25,657,354 (GRCm39)	S871P	probably damaging	Het

Other mutations in Or5g25

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02591:Or5g25	APN	2	85,478,487 (GRCm39)	missense	probably damaging	0.98
IGL02873:Or5g25	APN	2	85,478,096 (GRCm39)	missense	possibly damaging	0.50
PIT4362001:Or5g25	UTSW	2	85,478,068 (GRCm39)	missense	probably damaging	1.00
R1241:Or5g25	UTSW	2	85,477,904 (GRCm39)	missense	probably damaging	1.00
R1261:Or5g25	UTSW	2	85,478,143 (GRCm39)	missense	probably damaging	1.00
R1666:Or5g25	UTSW	2	85,478,157 (GRCm39)	nonsense	probably null
R1902:Or5g25	UTSW	2	85,478,201 (GRCm39)	missense	possibly damaging	0.81
R1965:Or5g25	UTSW	2	85,478,090 (GRCm39)	missense	possibly damaging	0.94
R2096:Or5g25	UTSW	2	85,478,434 (GRCm39)	missense	probably benign	0.20
R4239:Or5g25	UTSW	2	85,478,647 (GRCm39)	missense	probably damaging	0.98
R4730:Or5g25	UTSW	2	85,478,336 (GRCm39)	missense	probably benign	0.39
R4948:Or5g25	UTSW	2	85,477,916 (GRCm39)	missense	probably benign	0.30
R5627:Or5g25	UTSW	2	85,477,991 (GRCm39)	missense	probably damaging	1.00
R6809:Or5g25	UTSW	2	85,478,317 (GRCm39)	missense	probably damaging	1.00
R7399:Or5g25	UTSW	2	85,477,768 (GRCm39)	missense	possibly damaging	0.89
R7476:Or5g25	UTSW	2	85,478,512 (GRCm39)	missense	not run
R7805:Or5g25	UTSW	2	85,477,794 (GRCm39)	nonsense	probably null
R7960:Or5g25	UTSW	2	85,478,417 (GRCm39)	missense	possibly damaging	0.82
R8015:Or5g25	UTSW	2	85,478,136 (GRCm39)	missense	probably damaging	0.99
R8355:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8455:Or5g25	UTSW	2	85,478,485 (GRCm39)	missense	probably damaging	1.00
R8479:Or5g25	UTSW	2	85,478,447 (GRCm39)	missense	probably damaging	1.00
R8683:Or5g25	UTSW	2	85,478,410 (GRCm39)	missense	probably benign	0.35
R8699:Or5g25	UTSW	2	85,478,330 (GRCm39)	missense	possibly damaging	0.87
R8762:Or5g25	UTSW	2	85,478,034 (GRCm39)	missense	probably damaging	1.00
R8897:Or5g25	UTSW	2	85,478,187 (GRCm39)	missense	possibly damaging	0.92
R9280:Or5g25	UTSW	2	85,478,504 (GRCm39)	nonsense	probably null
R9674:Or5g25	UTSW	2	85,478,593 (GRCm39)	missense	possibly damaging	0.94

Predicted Primers

PCR Primer
(F):5'- TCCCAGCCATGATGAAAAGC -3'
(R):5'- TTCACCAGGACACTCGACTC -3'

Sequencing Primer
(F):5'- TCCCAGCCATGATGAAAAGCAAATG -3'
(R):5'- CTGTCATAAGTCCTAAGATGTTGTC -3'

Posted On

2016-12-20