Mutagenetix > Incidental Mutation

Incidental Mutation 'R5701:Gpr158'

450876

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

MMRRC Submission

043181-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5701 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21372378-21835355 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 21751520 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Cysteine at position 480 (R480C)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

probably damaging
Transcript: ENSMUST00000055946
AA Change: R480C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: R480C

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155760

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Armc8	A	G	9: 99,378,202 (GRCm39)		probably null	Het
Asxl1	A	G	2: 153,241,409 (GRCm39)	E653G	probably damaging	Het
AW551984	G	A	9: 39,504,118 (GRCm39)	A616V	probably benign	Het
Birc6	T	C	17: 75,004,420 (GRCm39)	M4763T	possibly damaging	Het
Bltp1	T	A	3: 36,975,509 (GRCm39)	M727K	probably benign	Het
Cand2	T	G	6: 115,774,893 (GRCm39)	F1034V	probably damaging	Het
Ccdc80	T	C	16: 44,936,741 (GRCm39)	V722A	possibly damaging	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Chrnd	G	A	1: 87,125,380 (GRCm39)	V340I	possibly damaging	Het
Clip1	T	C	5: 123,751,366 (GRCm39)		probably benign	Het
Csmd3	T	C	15: 47,513,617 (GRCm39)	E1920G	probably damaging	Het
Csmd3	A	T	15: 48,403,729 (GRCm39)	F194I	probably damaging	Het
D930048N14Rik	T	A	11: 51,544,556 (GRCm39)		probably null	Het
Defa28	T	C	8: 22,073,863 (GRCm39)	Y89H	probably benign	Het
Dnah1	C	T	14: 30,996,001 (GRCm39)	G2705E	probably damaging	Het
Eci2	A	T	13: 35,174,250 (GRCm39)	S104T	possibly damaging	Het
Ep300	T	C	15: 81,485,696 (GRCm39)	S228P	unknown	Het
Gabrb2	T	A	11: 42,378,201 (GRCm39)	M139K	probably damaging	Het
Gbp4	T	A	5: 105,266,265 (GRCm39)	N592I	possibly damaging	Het
Gps1	A	G	11: 120,676,008 (GRCm39)	D71G	probably benign	Het
Hoxc9	T	C	15: 102,890,313 (GRCm39)	Y77H	possibly damaging	Het
Il9r	T	A	11: 32,143,263 (GRCm39)	Y231F	probably benign	Het
Klhl1	A	G	14: 96,438,816 (GRCm39)	M494T	probably benign	Het
Kmt2c	A	G	5: 25,519,015 (GRCm39)	V2365A	probably benign	Het
Kri1	T	A	9: 21,192,425 (GRCm39)	K241M	possibly damaging	Het
Macf1	A	T	4: 123,397,018 (GRCm39)	C928S	probably damaging	Het
Mcm2	T	C	6: 88,870,073 (GRCm39)	D32G	probably damaging	Het
Mex3d	T	C	10: 80,217,379 (GRCm39)	T613A	probably benign	Het
Mptx2	G	A	1: 173,102,414 (GRCm39)	L92F	probably benign	Het
Myh9	T	C	15: 77,675,964 (GRCm39)	I241V	probably benign	Het
Myo6	C	T	9: 80,165,809 (GRCm39)	T398I	probably damaging	Het
Nckap1l	G	A	15: 103,381,195 (GRCm39)	A366T	probably benign	Het
Or5m11	A	G	2: 85,782,168 (GRCm39)	T254A	possibly damaging	Het
Plscr5	A	T	9: 92,087,564 (GRCm39)	K178*	probably null	Het
Rapgef6	T	C	11: 54,567,220 (GRCm39)	W857R	possibly damaging	Het
Raxos1	G	A	18: 66,071,059 (GRCm39)		probably benign	Het
Rnf145	G	A	11: 44,422,120 (GRCm39)	V68M	possibly damaging	Het
Slc66a2	T	A	18: 80,315,693 (GRCm39)	D118E	possibly damaging	Het
Synpo2	C	G	3: 122,873,879 (GRCm39)	G1029A	probably damaging	Het
Tbc1d5	TTGCTGCTGCTGCTGCTGCTGGTGCTGCTGCTGCTG	TTGCTGCTGCTGCTGCTGGTGCTGCTGCTGCTG	17: 51,106,983 (GRCm39)		probably benign	Het
Vmn2r14	T	C	5: 109,367,816 (GRCm39)	Y392C	probably damaging	Het
Vmn2r55	A	G	7: 12,404,492 (GRCm39)	F304L	probably benign	Het
Zfp316	C	T	5: 143,240,132 (GRCm39)	G629D	probably benign	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21,373,494 (GRCm39)	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21,751,606 (GRCm39)	splice site	probably benign
IGL00706:Gpr158	APN	2	21,751,584 (GRCm39)	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21,831,629 (GRCm39)	nonsense	probably null
IGL00885:Gpr158	APN	2	21,653,832 (GRCm39)	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21,373,842 (GRCm39)	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21,831,909 (GRCm39)	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21,788,101 (GRCm39)	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21,373,511 (GRCm39)	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21,831,407 (GRCm39)	missense	probably benign
IGL02681:Gpr158	APN	2	21,820,441 (GRCm39)	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21,831,638 (GRCm39)	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21,831,890 (GRCm39)	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21,787,972 (GRCm39)	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21,831,057 (GRCm39)	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21,831,682 (GRCm39)	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21,815,479 (GRCm39)	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21,831,528 (GRCm39)	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21,830,019 (GRCm39)	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21,820,480 (GRCm39)	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21,831,125 (GRCm39)	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21,820,505 (GRCm39)	splice site	probably null
R1609:Gpr158	UTSW	2	21,788,104 (GRCm39)	missense	possibly damaging	0.61
R1741:Gpr158	UTSW	2	21,832,359 (GRCm39)	missense	probably benign	0.00
R1827:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R1854:Gpr158	UTSW	2	21,373,935 (GRCm39)	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21,820,426 (GRCm39)	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21,832,325 (GRCm39)	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R2275:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R3004:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21,581,771 (GRCm39)	missense	possibly damaging	0.68
R3943:Gpr158	UTSW	2	21,373,370 (GRCm39)	missense	possibly damaging	0.65
R4238:Gpr158	UTSW	2	21,373,362 (GRCm39)	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21,830,025 (GRCm39)	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21,832,403 (GRCm39)	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4506:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21,373,811 (GRCm39)	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21,831,864 (GRCm39)	missense	probably benign
R4798:Gpr158	UTSW	2	21,787,993 (GRCm39)	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21,830,059 (GRCm39)	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21,831,968 (GRCm39)	missense	probably damaging	1.00
R5334:Gpr158	UTSW	2	21,832,316 (GRCm39)	missense	probably benign	0.01
R5560:Gpr158	UTSW	2	21,831,101 (GRCm39)	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21,832,046 (GRCm39)	missense	probably benign
R5637:Gpr158	UTSW	2	21,788,083 (GRCm39)	missense	probably benign	0.00
R5744:Gpr158	UTSW	2	21,373,331 (GRCm39)	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21,373,932 (GRCm39)	missense	possibly damaging	0.95
R5991:Gpr158	UTSW	2	21,373,319 (GRCm39)	missense	probably damaging	0.99
R6200:Gpr158	UTSW	2	21,404,227 (GRCm39)	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21,820,422 (GRCm39)	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21,815,365 (GRCm39)	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21,831,099 (GRCm39)	missense	probably damaging	1.00
R6698:Gpr158	UTSW	2	21,831,921 (GRCm39)	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21,653,802 (GRCm39)	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21,831,386 (GRCm39)	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21,373,113 (GRCm39)	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21,815,412 (GRCm39)	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21,581,750 (GRCm39)	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R7515:Gpr158	UTSW	2	21,373,092 (GRCm39)	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21,831,158 (GRCm39)	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R8311:Gpr158	UTSW	2	21,373,701 (GRCm39)	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21,581,693 (GRCm39)	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21,404,149 (GRCm39)	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21,558,137 (GRCm39)	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21,581,751 (GRCm39)	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21,581,760 (GRCm39)	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21,830,078 (GRCm39)	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21,831,251 (GRCm39)	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9317:Gpr158	UTSW	2	21,832,037 (GRCm39)	missense	probably benign
R9379:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9428:Gpr158	UTSW	2	21,787,972 (GRCm39)	missense	probably benign
R9497:Gpr158	UTSW	2	21,831,825 (GRCm39)	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21,830,054 (GRCm39)	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21,831,315 (GRCm39)	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21,831,180 (GRCm39)	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21,815,501 (GRCm39)	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21,832,083 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- ACTACAGACTTGGAAACGTGATC -3'
(R):5'- ACATTGTTGAATAATGGCCAGC -3'

Sequencing Primer
(F):5'- GAGTGATCCTGTAGTTCCTCAGC -3'
(R):5'- TGGCCAGCTCAGTTTACAAC -3'

Posted On

2017-01-03