Mutagenetix > Incidental Mutation

Incidental Mutation 'R5701:Vmn2r14'

450884

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r14

Ensembl Gene

ENSMUSG00000091059

Gene Name

vomeronasal 2, receptor 14

Synonyms

EG231591

MMRRC Submission

043181-MU

Accession Numbers

Essential gene?

Possibly non essential (E-score: 0.469) question?

Stock #

R5701 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

109362822-109372488 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 109367816 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Cysteine at position 392 (Y392C)

Ref Sequence

ENSEMBL: ENSMUSP00000128015 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170341]

AlphaFold

E9Q759

Predicted Effect

probably damaging
Transcript: ENSMUST00000170341
AA Change: Y392C

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000128015
Gene: ENSMUSG00000091059
AA Change: Y392C

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
Pfam:ANF_receptor	76	466	8.3e-31	PFAM
Pfam:NCD3G	507	561	1.1e-17	PFAM
Pfam:7tm_3	594	829	1.2e-55	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.1%
20x: 94.9%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Armc8	A	G	9: 99,378,202 (GRCm39)		probably null	Het
Asxl1	A	G	2: 153,241,409 (GRCm39)	E653G	probably damaging	Het
AW551984	G	A	9: 39,504,118 (GRCm39)	A616V	probably benign	Het
Birc6	T	C	17: 75,004,420 (GRCm39)	M4763T	possibly damaging	Het
Bltp1	T	A	3: 36,975,509 (GRCm39)	M727K	probably benign	Het
Cand2	T	G	6: 115,774,893 (GRCm39)	F1034V	probably damaging	Het
Ccdc80	T	C	16: 44,936,741 (GRCm39)	V722A	possibly damaging	Het
Cd109	CATTTATTTATTTATTTATTTATTTATTTATTTAT	CATTTATTTATTTATTTATTTATTTATTTATTTATTTAT	9: 78,619,782 (GRCm39)		probably benign	Het
Chrnd	G	A	1: 87,125,380 (GRCm39)	V340I	possibly damaging	Het
Clip1	T	C	5: 123,751,366 (GRCm39)		probably benign	Het
Csmd3	T	C	15: 47,513,617 (GRCm39)	E1920G	probably damaging	Het
Csmd3	A	T	15: 48,403,729 (GRCm39)	F194I	probably damaging	Het
D930048N14Rik	T	A	11: 51,544,556 (GRCm39)		probably null	Het
Defa28	T	C	8: 22,073,863 (GRCm39)	Y89H	probably benign	Het
Dnah1	C	T	14: 30,996,001 (GRCm39)	G2705E	probably damaging	Het
Eci2	A	T	13: 35,174,250 (GRCm39)	S104T	possibly damaging	Het
Ep300	T	C	15: 81,485,696 (GRCm39)	S228P	unknown	Het
Gabrb2	T	A	11: 42,378,201 (GRCm39)	M139K	probably damaging	Het
Gbp4	T	A	5: 105,266,265 (GRCm39)	N592I	possibly damaging	Het
Gpr158	C	T	2: 21,751,520 (GRCm39)	R480C	probably damaging	Het
Gps1	A	G	11: 120,676,008 (GRCm39)	D71G	probably benign	Het
Hoxc9	T	C	15: 102,890,313 (GRCm39)	Y77H	possibly damaging	Het
Il9r	T	A	11: 32,143,263 (GRCm39)	Y231F	probably benign	Het
Klhl1	A	G	14: 96,438,816 (GRCm39)	M494T	probably benign	Het
Kmt2c	A	G	5: 25,519,015 (GRCm39)	V2365A	probably benign	Het
Kri1	T	A	9: 21,192,425 (GRCm39)	K241M	possibly damaging	Het
Macf1	A	T	4: 123,397,018 (GRCm39)	C928S	probably damaging	Het
Mcm2	T	C	6: 88,870,073 (GRCm39)	D32G	probably damaging	Het
Mex3d	T	C	10: 80,217,379 (GRCm39)	T613A	probably benign	Het
Mptx2	G	A	1: 173,102,414 (GRCm39)	L92F	probably benign	Het
Myh9	T	C	15: 77,675,964 (GRCm39)	I241V	probably benign	Het
Myo6	C	T	9: 80,165,809 (GRCm39)	T398I	probably damaging	Het
Nckap1l	G	A	15: 103,381,195 (GRCm39)	A366T	probably benign	Het
Or5m11	A	G	2: 85,782,168 (GRCm39)	T254A	possibly damaging	Het
Plscr5	A	T	9: 92,087,564 (GRCm39)	K178*	probably null	Het
Rapgef6	T	C	11: 54,567,220 (GRCm39)	W857R	possibly damaging	Het
Raxos1	G	A	18: 66,071,059 (GRCm39)		probably benign	Het
Rnf145	G	A	11: 44,422,120 (GRCm39)	V68M	possibly damaging	Het
Slc66a2	T	A	18: 80,315,693 (GRCm39)	D118E	possibly damaging	Het
Synpo2	C	G	3: 122,873,879 (GRCm39)	G1029A	probably damaging	Het
Tbc1d5	TTGCTGCTGCTGCTGCTGCTGGTGCTGCTGCTGCTG	TTGCTGCTGCTGCTGCTGGTGCTGCTGCTGCTG	17: 51,106,983 (GRCm39)		probably benign	Het
Vmn2r55	A	G	7: 12,404,492 (GRCm39)	F304L	probably benign	Het
Zfp316	C	T	5: 143,240,132 (GRCm39)	G629D	probably benign	Het

Other mutations in Vmn2r14

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01472:Vmn2r14	APN	5	109,364,180 (GRCm39)	nonsense	probably null
IGL01504:Vmn2r14	APN	5	109,369,285 (GRCm39)	missense	probably benign	0.01
IGL01828:Vmn2r14	APN	5	109,372,443 (GRCm39)	missense	possibly damaging	0.71
IGL02093:Vmn2r14	APN	5	109,368,275 (GRCm39)	missense	possibly damaging	0.94
IGL02103:Vmn2r14	APN	5	109,372,349 (GRCm39)	missense	probably damaging	0.96
IGL02123:Vmn2r14	APN	5	109,367,933 (GRCm39)	missense	probably damaging	1.00
IGL02145:Vmn2r14	APN	5	109,368,454 (GRCm39)	nonsense	probably null
IGL02676:Vmn2r14	APN	5	109,367,882 (GRCm39)	missense	probably benign	0.03
IGL02720:Vmn2r14	APN	5	109,369,305 (GRCm39)	missense	probably damaging	1.00
IGL02877:Vmn2r14	APN	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
IGL02974:Vmn2r14	APN	5	109,369,292 (GRCm39)	missense	possibly damaging	0.55
IGL03151:Vmn2r14	APN	5	109,364,260 (GRCm39)	missense	probably damaging	1.00
IGL03297:Vmn2r14	APN	5	109,363,973 (GRCm39)	missense	probably damaging	1.00
IGL03386:Vmn2r14	APN	5	109,368,350 (GRCm39)	missense	possibly damaging	0.90
IGL03394:Vmn2r14	APN	5	109,367,702 (GRCm39)	missense	probably null	0.83
ANU74:Vmn2r14	UTSW	5	109,366,910 (GRCm39)	missense	probably benign	0.00
R0316:Vmn2r14	UTSW	5	109,366,762 (GRCm39)	missense	probably benign	0.07
R0755:Vmn2r14	UTSW	5	109,364,226 (GRCm39)	missense	possibly damaging	0.81
R1219:Vmn2r14	UTSW	5	109,372,440 (GRCm39)	missense	probably benign	0.17
R1321:Vmn2r14	UTSW	5	109,364,117 (GRCm39)	missense	probably benign	0.08
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1465:Vmn2r14	UTSW	5	109,368,195 (GRCm39)	missense	possibly damaging	0.47
R1509:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R1551:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	probably damaging	1.00
R1628:Vmn2r14	UTSW	5	109,367,838 (GRCm39)	missense	probably benign	0.00
R1668:Vmn2r14	UTSW	5	109,366,913 (GRCm39)	nonsense	probably null
R2013:Vmn2r14	UTSW	5	109,369,109 (GRCm39)	missense	probably benign	0.00
R2201:Vmn2r14	UTSW	5	109,366,698 (GRCm39)	splice site	probably null
R2417:Vmn2r14	UTSW	5	109,372,329 (GRCm39)	missense	probably benign	0.00
R3029:Vmn2r14	UTSW	5	109,363,776 (GRCm39)	missense	probably damaging	1.00
R3120:Vmn2r14	UTSW	5	109,372,431 (GRCm39)	missense	probably null	0.00
R3729:Vmn2r14	UTSW	5	109,364,095 (GRCm39)	missense	probably damaging	1.00
R3762:Vmn2r14	UTSW	5	109,368,033 (GRCm39)	missense	probably benign	0.02
R3943:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R3944:Vmn2r14	UTSW	5	109,363,930 (GRCm39)	missense	probably damaging	1.00
R4222:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4224:Vmn2r14	UTSW	5	109,364,149 (GRCm39)	missense	probably benign	0.00
R4239:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4240:Vmn2r14	UTSW	5	109,364,277 (GRCm39)	critical splice acceptor site	probably null
R4782:Vmn2r14	UTSW	5	109,369,370 (GRCm39)	missense	probably benign	0.01
R4832:Vmn2r14	UTSW	5	109,363,976 (GRCm39)	missense	probably damaging	1.00
R4884:Vmn2r14	UTSW	5	109,369,384 (GRCm39)	splice site	probably null
R4896:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5004:Vmn2r14	UTSW	5	109,368,246 (GRCm39)	missense	probably benign	0.19
R5117:Vmn2r14	UTSW	5	109,363,961 (GRCm39)	missense	probably benign	0.16
R5285:Vmn2r14	UTSW	5	109,365,442 (GRCm39)	missense	probably damaging	0.98
R5413:Vmn2r14	UTSW	5	109,369,154 (GRCm39)	missense	probably benign	0.29
R5569:Vmn2r14	UTSW	5	109,368,261 (GRCm39)	missense	probably benign	0.44
R5726:Vmn2r14	UTSW	5	109,365,486 (GRCm39)	missense	possibly damaging	0.95
R5763:Vmn2r14	UTSW	5	109,363,724 (GRCm39)	missense	possibly damaging	0.49
R5872:Vmn2r14	UTSW	5	109,369,222 (GRCm39)	missense	probably benign
R5985:Vmn2r14	UTSW	5	109,368,082 (GRCm39)	missense	possibly damaging	0.89
R6268:Vmn2r14	UTSW	5	109,369,283 (GRCm39)	missense	possibly damaging	0.87
R6273:Vmn2r14	UTSW	5	109,369,133 (GRCm39)	missense	probably benign	0.44
R6409:Vmn2r14	UTSW	5	109,364,096 (GRCm39)	missense	probably benign	0.09
R6944:Vmn2r14	UTSW	5	109,364,140 (GRCm39)	missense	probably benign	0.06
R6944:Vmn2r14	UTSW	5	109,363,925 (GRCm39)	missense	probably benign	0.22
R7608:Vmn2r14	UTSW	5	109,369,276 (GRCm39)	missense	probably benign	0.03
R7740:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7768:Vmn2r14	UTSW	5	109,368,086 (GRCm39)	missense	probably benign	0.01
R7804:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R7872:Vmn2r14	UTSW	5	109,369,219 (GRCm39)	missense	probably benign	0.02
R7993:Vmn2r14	UTSW	5	109,363,862 (GRCm39)	missense	probably benign	0.00
R8006:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8007:Vmn2r14	UTSW	5	109,368,324 (GRCm39)	missense	probably benign	0.41
R8187:Vmn2r14	UTSW	5	109,368,420 (GRCm39)	missense	probably benign	0.03
R8369:Vmn2r14	UTSW	5	109,369,342 (GRCm39)	missense	probably damaging	1.00
R8463:Vmn2r14	UTSW	5	109,369,340 (GRCm39)	missense	probably benign	0.30
R8968:Vmn2r14	UTSW	5	109,365,533 (GRCm39)	missense	probably benign	0.01
R9008:Vmn2r14	UTSW	5	109,367,893 (GRCm39)	missense	probably benign	0.00
R9030:Vmn2r14	UTSW	5	109,368,054 (GRCm39)	missense	probably damaging	0.99
R9039:Vmn2r14	UTSW	5	109,367,902 (GRCm39)	nonsense	probably null
R9150:Vmn2r14	UTSW	5	109,367,783 (GRCm39)	missense	probably damaging	1.00
R9164:Vmn2r14	UTSW	5	109,364,087 (GRCm39)	missense	probably damaging	1.00
R9216:Vmn2r14	UTSW	5	109,369,112 (GRCm39)	missense	probably benign	0.01
R9225:Vmn2r14	UTSW	5	109,369,288 (GRCm39)	missense	probably damaging	1.00
R9245:Vmn2r14	UTSW	5	109,368,176 (GRCm39)	missense	possibly damaging	0.89
R9342:Vmn2r14	UTSW	5	109,368,428 (GRCm39)	missense	probably damaging	1.00
R9472:Vmn2r14	UTSW	5	109,367,962 (GRCm39)	missense	probably benign	0.00
R9678:Vmn2r14	UTSW	5	109,364,041 (GRCm39)	missense	probably damaging	1.00
R9774:Vmn2r14	UTSW	5	109,369,126 (GRCm39)	missense	probably benign	0.07
Z1177:Vmn2r14	UTSW	5	109,367,741 (GRCm39)	missense	probably benign	0.33

Predicted Primers

PCR Primer
(F):5'- TCTCTGGGTCCATTTAAAAGATTCC -3'
(R):5'- GGACTGTCACTTTTGAATACCACC -3'

Sequencing Primer
(F):5'- GCTGACAGTCAGTGAATGT -3'
(R):5'- GTCACTTTTGAATACCACCACAGTG -3'

Posted On

2017-01-03