Mutagenetix > Incidental Mutation

Incidental Mutation 'R5714:Zfp97'

451070

Institutional Source

Beutler Lab

Gene Symbol

Zfp97

Ensembl Gene

ENSMUSG00000095990

Gene Name

zinc finger protein 97

Synonyms

MMRRC Submission

043186-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.152) question?

Stock #

R5714 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

17341677-17367093 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 17365871 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 457 (T457A)

Ref Sequence

ENSEMBL: ENSMUSP00000114301 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000147630]

AlphaFold

E9PZP6

Predicted Effect

possibly damaging
Transcript: ENSMUST00000147630
AA Change: T457A

PolyPhen 2 Score 0.861 (Sensitivity: 0.83; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000114301
Gene: ENSMUSG00000095990
AA Change: T457A

Domain	Start	End	E-Value	Type
KRAB	13	82	4.42e-16	SMART
ZnF_C2H2	158	180	6.88e-4	SMART
ZnF_C2H2	186	208	4.4e-2	SMART
ZnF_C2H2	236	260	4.57e0	SMART
ZnF_C2H2	266	288	6.32e-3	SMART
ZnF_C2H2	294	316	8.94e-3	SMART
ZnF_C2H2	322	344	2.53e-2	SMART
ZnF_C2H2	350	372	9.73e-4	SMART
ZnF_C2H2	378	400	3.89e-3	SMART
ZnF_C2H2	406	428	8.94e-3	SMART
ZnF_C2H2	434	456	1.18e-2	SMART
ZnF_C2H2	462	484	7.9e-4	SMART
ZnF_C2H2	490	512	7.78e-3	SMART
ZnF_C2H2	518	540	3.95e-4	SMART

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alpk2	G	T	18: 65,438,532 (GRCm39)	Q1421K	possibly damaging	Het
Ankub1	G	A	3: 57,580,258 (GRCm39)	T133M	probably benign	Het
Bcar3	G	A	3: 122,248,736 (GRCm39)	V112M	possibly damaging	Het
Cacna1s	A	G	1: 136,039,804 (GRCm39)	K1476R	probably benign	Het
Cdc42ep1	C	A	15: 78,733,977 (GRCm39)	A359E	possibly damaging	Het
Cemip	T	A	7: 83,624,387 (GRCm39)	D483V	probably damaging	Het
Cyp4f39	G	A	17: 32,700,799 (GRCm39)	R156H	probably damaging	Het
Dpys	T	A	15: 39,720,553 (GRCm39)	H69L	probably damaging	Het
Fbxl21	A	G	13: 56,674,885 (GRCm39)	I79V	probably benign	Het
Fgf9	T	C	14: 58,347,022 (GRCm39)	L205P	probably damaging	Het
Fkbp14	A	G	6: 54,562,835 (GRCm39)	F144L	probably damaging	Het
Flnb	A	C	14: 7,929,073 (GRCm38)	D1934A	probably damaging	Het
Garre1	G	A	7: 33,939,941 (GRCm39)	Q717*	probably null	Het
Glul	T	C	1: 153,782,243 (GRCm39)		probably benign	Het
Helz	T	A	11: 107,517,347 (GRCm39)		probably null	Het
Kap	A	G	6: 133,828,956 (GRCm39)	Y42H	probably benign	Het
Kcnh8	T	C	17: 53,285,150 (GRCm39)	V1040A	probably benign	Het
Kptn	A	G	7: 15,854,683 (GRCm39)		probably null	Het
Lypd3	A	G	7: 24,338,494 (GRCm39)	T182A	possibly damaging	Het
Mcm5	A	C	8: 75,847,538 (GRCm39)	D445A	probably damaging	Het
Mios	T	A	6: 8,215,434 (GRCm39)	I210K	probably damaging	Het
Nat8f2	A	G	6: 85,844,891 (GRCm39)	V157A	probably benign	Het
Or1e16	T	A	11: 73,286,187 (GRCm39)		probably null	Het
Pard3b	C	A	1: 62,677,075 (GRCm39)	A1140E	probably null	Het
Pcnt	A	T	10: 76,256,325 (GRCm39)	D638E	probably damaging	Het
Pdgfra	T	C	5: 75,346,673 (GRCm39)	I834T	probably damaging	Het
Phyhd1	T	A	2: 30,169,994 (GRCm39)	L162H	probably damaging	Het
Polg	C	A	7: 79,101,739 (GRCm39)	A1026S	possibly damaging	Het
Prl7c1	A	T	13: 27,962,949 (GRCm39)	L18*	probably null	Het
Rars2	T	A	4: 34,645,779 (GRCm39)	M232K	probably benign	Het
Rtl1	A	G	12: 109,560,114 (GRCm39)	V575A	probably damaging	Het
Slc18b1	A	T	10: 23,674,664 (GRCm39)	T40S	probably benign	Het
Slc22a19	T	C	19: 7,688,387 (GRCm39)	T58A	probably damaging	Het
Slc27a6	A	T	18: 58,731,625 (GRCm39)	E325V	probably damaging	Het
Slc35e2	C	T	4: 155,694,483 (GRCm39)	P10L	probably benign	Het
Srd5a3	T	A	5: 76,301,413 (GRCm39)	F214Y	probably benign	Het
Tgfbr2	A	T	9: 116,004,092 (GRCm39)	L5Q	probably damaging	Het
Tmem94	C	A	11: 115,684,016 (GRCm39)	Q779K	probably benign	Het
Ttn	G	A	2: 76,652,837 (GRCm39)	P216S	probably benign	Het
Ush2a	A	T	1: 188,132,454 (GRCm39)	Q892L	probably benign	Het
Vgll4	A	T	6: 114,867,737 (GRCm39)	M38K	possibly damaging	Het
Vmn2r109	A	G	17: 20,773,121 (GRCm39)	I500T	probably damaging	Het
Vps33a	T	C	5: 123,707,563 (GRCm39)	I135V	probably benign	Het
Wscd1	T	C	11: 71,675,261 (GRCm39)		probably null	Het
Zfp457	C	A	13: 67,444,490 (GRCm39)	M4I	possibly damaging	Het
Zfp532	G	A	18: 65,756,606 (GRCm39)	G180R	possibly damaging	Het

Other mutations in Zfp97

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
R0812:Zfp97	UTSW	17	17,365,552 (GRCm39)	missense	possibly damaging	0.77
R1550:Zfp97	UTSW	17	17,365,468 (GRCm39)	nonsense	probably null
R1920:Zfp97	UTSW	17	17,365,265 (GRCm39)	missense	probably benign	0.00
R2058:Zfp97	UTSW	17	17,365,018 (GRCm39)	missense	possibly damaging	0.57
R4896:Zfp97	UTSW	17	17,365,038 (GRCm39)	missense	probably benign	0.09
R4967:Zfp97	UTSW	17	17,364,938 (GRCm39)	nonsense	probably null
R4967:Zfp97	UTSW	17	17,365,393 (GRCm39)	missense	probably damaging	1.00
R5341:Zfp97	UTSW	17	17,365,472 (GRCm39)	missense	probably damaging	1.00
R5866:Zfp97	UTSW	17	17,365,087 (GRCm39)	missense	possibly damaging	0.79
R6861:Zfp97	UTSW	17	17,365,437 (GRCm39)	missense	probably damaging	1.00
R7506:Zfp97	UTSW	17	17,365,542 (GRCm39)	missense	probably damaging	1.00
R9136:Zfp97	UTSW	17	17,365,215 (GRCm39)	missense	probably benign	0.00
R9516:Zfp97	UTSW	17	17,365,930 (GRCm39)	missense	possibly damaging	0.90
R9580:Zfp97	UTSW	17	17,365,243 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- CAAAGCCTTTGCATGTCCCAG -3'
(R):5'- TAAAAGCTTTGCTACACTGGTTAA -3'

Sequencing Primer
(F):5'- TGCATGTCCCAGATACCTTCAAATC -3'
(R):5'- GCCACACTGGTTACATTCATAGGG -3'

Posted On

2017-01-03