Mutagenetix > Incidental Mutation

Incidental Mutation 'R5714:Vmn2r109'

451071

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

043186-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R5714 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20760779-20785018 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20773121 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Threonine at position 500 (I500T)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably damaging
Transcript: ENSMUST00000167093
AA Change: I500T

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: I500T

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Alpk2	G	T	18: 65,438,532 (GRCm39)	Q1421K	possibly damaging	Het
Ankub1	G	A	3: 57,580,258 (GRCm39)	T133M	probably benign	Het
Bcar3	G	A	3: 122,248,736 (GRCm39)	V112M	possibly damaging	Het
Cacna1s	A	G	1: 136,039,804 (GRCm39)	K1476R	probably benign	Het
Cdc42ep1	C	A	15: 78,733,977 (GRCm39)	A359E	possibly damaging	Het
Cemip	T	A	7: 83,624,387 (GRCm39)	D483V	probably damaging	Het
Cyp4f39	G	A	17: 32,700,799 (GRCm39)	R156H	probably damaging	Het
Dpys	T	A	15: 39,720,553 (GRCm39)	H69L	probably damaging	Het
Fbxl21	A	G	13: 56,674,885 (GRCm39)	I79V	probably benign	Het
Fgf9	T	C	14: 58,347,022 (GRCm39)	L205P	probably damaging	Het
Fkbp14	A	G	6: 54,562,835 (GRCm39)	F144L	probably damaging	Het
Flnb	A	C	14: 7,929,073 (GRCm38)	D1934A	probably damaging	Het
Garre1	G	A	7: 33,939,941 (GRCm39)	Q717*	probably null	Het
Glul	T	C	1: 153,782,243 (GRCm39)		probably benign	Het
Helz	T	A	11: 107,517,347 (GRCm39)		probably null	Het
Kap	A	G	6: 133,828,956 (GRCm39)	Y42H	probably benign	Het
Kcnh8	T	C	17: 53,285,150 (GRCm39)	V1040A	probably benign	Het
Kptn	A	G	7: 15,854,683 (GRCm39)		probably null	Het
Lypd3	A	G	7: 24,338,494 (GRCm39)	T182A	possibly damaging	Het
Mcm5	A	C	8: 75,847,538 (GRCm39)	D445A	probably damaging	Het
Mios	T	A	6: 8,215,434 (GRCm39)	I210K	probably damaging	Het
Nat8f2	A	G	6: 85,844,891 (GRCm39)	V157A	probably benign	Het
Or1e16	T	A	11: 73,286,187 (GRCm39)		probably null	Het
Pard3b	C	A	1: 62,677,075 (GRCm39)	A1140E	probably null	Het
Pcnt	A	T	10: 76,256,325 (GRCm39)	D638E	probably damaging	Het
Pdgfra	T	C	5: 75,346,673 (GRCm39)	I834T	probably damaging	Het
Phyhd1	T	A	2: 30,169,994 (GRCm39)	L162H	probably damaging	Het
Polg	C	A	7: 79,101,739 (GRCm39)	A1026S	possibly damaging	Het
Prl7c1	A	T	13: 27,962,949 (GRCm39)	L18*	probably null	Het
Rars2	T	A	4: 34,645,779 (GRCm39)	M232K	probably benign	Het
Rtl1	A	G	12: 109,560,114 (GRCm39)	V575A	probably damaging	Het
Slc18b1	A	T	10: 23,674,664 (GRCm39)	T40S	probably benign	Het
Slc22a19	T	C	19: 7,688,387 (GRCm39)	T58A	probably damaging	Het
Slc27a6	A	T	18: 58,731,625 (GRCm39)	E325V	probably damaging	Het
Slc35e2	C	T	4: 155,694,483 (GRCm39)	P10L	probably benign	Het
Srd5a3	T	A	5: 76,301,413 (GRCm39)	F214Y	probably benign	Het
Tgfbr2	A	T	9: 116,004,092 (GRCm39)	L5Q	probably damaging	Het
Tmem94	C	A	11: 115,684,016 (GRCm39)	Q779K	probably benign	Het
Ttn	G	A	2: 76,652,837 (GRCm39)	P216S	probably benign	Het
Ush2a	A	T	1: 188,132,454 (GRCm39)	Q892L	probably benign	Het
Vgll4	A	T	6: 114,867,737 (GRCm39)	M38K	possibly damaging	Het
Vps33a	T	C	5: 123,707,563 (GRCm39)	I135V	probably benign	Het
Wscd1	T	C	11: 71,675,261 (GRCm39)		probably null	Het
Zfp457	C	A	13: 67,444,490 (GRCm39)	M4I	possibly damaging	Het
Zfp532	G	A	18: 65,756,606 (GRCm39)	G180R	possibly damaging	Het
Zfp97	A	G	17: 17,365,871 (GRCm39)	T457A	possibly damaging	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20,770,419 (GRCm39)	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20,761,383 (GRCm39)	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20,761,671 (GRCm39)	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20,774,654 (GRCm39)	missense	probably benign
IGL01864:Vmn2r109	APN	17	20,761,396 (GRCm39)	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20,761,342 (GRCm39)	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20,774,603 (GRCm39)	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20,774,422 (GRCm39)	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20,761,150 (GRCm39)	missense	probably benign
IGL02490:Vmn2r109	APN	17	20,761,246 (GRCm39)	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20,760,963 (GRCm39)	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20,774,518 (GRCm39)	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20,774,062 (GRCm39)	missense	probably benign
IGL02745:Vmn2r109	APN	17	20,761,512 (GRCm39)	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20,774,839 (GRCm39)	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20,773,148 (GRCm39)	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20,760,937 (GRCm39)	missense	probably damaging	0.99
R0855:Vmn2r109	UTSW	17	20,761,670 (GRCm39)	nonsense	probably null
R0882:Vmn2r109	UTSW	17	20,774,842 (GRCm39)	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20,775,503 (GRCm39)	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20,761,002 (GRCm39)	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20,774,072 (GRCm39)	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20,774,185 (GRCm39)	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20,761,448 (GRCm39)	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20,784,974 (GRCm39)	missense	probably benign	0.00
R2436:Vmn2r109	UTSW	17	20,774,798 (GRCm39)	missense	probably damaging	0.99
R3123:Vmn2r109	UTSW	17	20,761,248 (GRCm39)	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20,774,704 (GRCm39)	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20,774,074 (GRCm39)	missense	probably benign
R4428:Vmn2r109	UTSW	17	20,773,286 (GRCm39)	missense	probably benign
R4584:Vmn2r109	UTSW	17	20,774,820 (GRCm39)	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20,761,656 (GRCm39)	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20,761,605 (GRCm39)	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20,774,153 (GRCm39)	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20,761,494 (GRCm39)	missense	probably benign	0.01
R4907:Vmn2r109	UTSW	17	20,770,348 (GRCm39)	missense	probably damaging	1.00
R5011:Vmn2r109	UTSW	17	20,775,451 (GRCm39)	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20,774,603 (GRCm39)	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20,761,189 (GRCm39)	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20,760,933 (GRCm39)	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20,760,781 (GRCm39)	makesense	probably null
R5702:Vmn2r109	UTSW	17	20,774,407 (GRCm39)	missense	probably benign	0.42
R5706:Vmn2r109	UTSW	17	20,774,567 (GRCm39)	missense	probably benign	0.16
R5832:Vmn2r109	UTSW	17	20,761,318 (GRCm39)	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20,760,981 (GRCm39)	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20,761,440 (GRCm39)	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20,784,796 (GRCm39)	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20,774,785 (GRCm39)	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20,760,932 (GRCm39)	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20,760,973 (GRCm39)	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20,785,006 (GRCm39)	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20,761,225 (GRCm39)	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20,760,945 (GRCm39)	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20,761,700 (GRCm39)	missense	probably benign	0.05
R7354:Vmn2r109	UTSW	17	20,761,043 (GRCm39)	missense	probably damaging	1.00
R7357:Vmn2r109	UTSW	17	20,761,536 (GRCm39)	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20,774,665 (GRCm39)	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20,760,942 (GRCm39)	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20,773,117 (GRCm39)	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20,761,436 (GRCm39)	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20,760,782 (GRCm39)	missense	probably benign	0.08
R8113:Vmn2r109	UTSW	17	20,774,729 (GRCm39)	missense	probably benign	0.01
R8153:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20,774,531 (GRCm39)	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20,775,332 (GRCm39)	missense
Z1176:Vmn2r109	UTSW	17	20,773,256 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- ACTGCAAGCCTTATTTCACAC -3'
(R):5'- TCTGCTTCTTGGACACAGTG -3'

Sequencing Primer
(F):5'- CTTCCTCTAATCTGAATGAAGGAAC -3'
(R):5'- TTTCCTGAAGGACATTGATGAGAG -3'

Posted On

2017-01-03