Mutagenetix > Incidental Mutation

Incidental Mutation 'R5716:Muc21'

451197

Institutional Source

Beutler Lab

Gene Symbol

Muc21

Ensembl Gene

ENSMUSG00000090588

Gene Name

mucin 21

Synonyms

epiglycanin, Gm9573

MMRRC Submission

043187-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.097) question?

Stock #

R5716 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

35928815-35937529 bp(-) (GRCm39)

Type of Mutation

intron

DNA Base Change (assembly)

A to C at 35931675 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000134221 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000164502] [ENSMUST00000174521]

AlphaFold

F7C950

Predicted Effect

unknown
Transcript: ENSMUST00000164502
AA Change: L837R

SMART Domains

Protein: ENSMUSP00000130987
Gene: ENSMUSG00000090588
AA Change: L837R

Domain	Start	End	E-Value	Type
signal peptide	1	23	N/A	INTRINSIC
low complexity region	26	39	N/A	INTRINSIC
low complexity region	42	59	N/A	INTRINSIC
low complexity region	76	144	N/A	INTRINSIC
low complexity region	149	578	N/A	INTRINSIC
low complexity region	580	653	N/A	INTRINSIC
low complexity region	655	1179	N/A	INTRINSIC
low complexity region	1183	1373	N/A	INTRINSIC
low complexity region	1383	1436	N/A	INTRINSIC
low complexity region	1438	1479	N/A	INTRINSIC
Pfam:Epiglycanin_C	1518	1605	3.8e-22	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000173759

Predicted Effect

probably benign
Transcript: ENSMUST00000174521

SMART Domains

Protein: ENSMUSP00000134221
Gene: ENSMUSG00000090509

Domain	Start	End	E-Value	Type
Pfam:SFTA2	80	117	9.2e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174534

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.2%
20x: 95.2%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 40 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1810065E05Rik	T	C	11: 58,312,594 (GRCm39)	V24A	possibly damaging	Het
Abca14	G	A	7: 119,846,217 (GRCm39)		probably null	Het
Acad12	A	G	5: 121,748,046 (GRCm39)	V124A	probably benign	Het
Alg1	A	T	16: 5,057,820 (GRCm39)	D238V	probably damaging	Het
Bcar3	A	G	3: 122,306,564 (GRCm39)	E179G	probably damaging	Het
Brf2	A	G	8: 27,616,074 (GRCm39)	S104P	probably benign	Het
Coq8a	A	T	1: 180,006,825 (GRCm39)	Y21N	possibly damaging	Het
Cramp1	A	G	17: 25,193,709 (GRCm39)	F924L	probably damaging	Het
Dpyd	G	T	3: 118,692,828 (GRCm39)	C324F	probably damaging	Het
Eif4b	T	C	15: 101,990,494 (GRCm39)	Y33H	probably benign	Het
Fry	C	T	5: 150,293,686 (GRCm39)	Q460*	probably null	Het
Fryl	T	C	5: 73,257,808 (GRCm39)	I665V	probably benign	Het
Gpank1	A	G	17: 35,342,229 (GRCm39)	K90E	probably damaging	Het
Hexd	A	G	11: 121,112,388 (GRCm39)	I482V	probably benign	Het
Hmcn2	G	A	2: 31,226,579 (GRCm39)	E185K	probably damaging	Het
Hmcn2	A	G	2: 31,348,750 (GRCm39)	E4922G	possibly damaging	Het
Ino80d	A	T	1: 63,097,856 (GRCm39)	D679E	probably benign	Het
Kalrn	A	T	16: 33,807,546 (GRCm39)	C2608S	probably benign	Het
Kars1	T	A	8: 112,730,074 (GRCm39)		probably null	Het
Lcn2	A	G	2: 32,275,825 (GRCm39)	V211A	possibly damaging	Het
Lsmem1	A	T	12: 40,230,692 (GRCm39)	V70E	possibly damaging	Het
Med12l	T	C	3: 59,208,798 (GRCm39)		probably null	Het
Megf11	T	C	9: 64,413,392 (GRCm39)	F60L	possibly damaging	Het
Neb	T	A	2: 52,100,596 (GRCm39)	H4438L	probably benign	Het
Nuf2	C	A	1: 169,349,958 (GRCm39)	V107F	probably benign	Het
Or14j6	A	T	17: 38,214,719 (GRCm39)	Y94F	probably benign	Het
Or6c8	T	A	10: 128,915,424 (GRCm39)	N136I	probably benign	Het
Or9i2	C	T	19: 13,816,003 (GRCm39)	C178Y	probably damaging	Het
Pabpn1l	A	G	8: 123,347,160 (GRCm39)	V215A	probably damaging	Het
Pnn	A	G	12: 59,118,658 (GRCm39)	I414V	probably benign	Het
Rab11fip3	A	G	17: 26,255,638 (GRCm39)	Y539H	probably damaging	Het
Rassf4	A	G	6: 116,638,828 (GRCm39)	V13A	probably benign	Het
Sephs1	T	C	2: 4,889,389 (GRCm39)	F56L	probably benign	Het
Sh3rf3	C	T	10: 58,967,105 (GRCm39)	P816S	probably benign	Het
Skint10	A	G	4: 112,568,844 (GRCm39)	L291P	probably damaging	Het
Thsd7a	T	A	6: 12,343,147 (GRCm39)	I1157L	probably benign	Het
Tmem184c	T	C	8: 78,333,036 (GRCm39)	H85R	possibly damaging	Het
Tmem94	T	C	11: 115,683,254 (GRCm39)	V679A	probably benign	Het
Tpcn2	A	C	7: 144,811,550 (GRCm39)	F566V	possibly damaging	Het
Uqcrc1	A	G	9: 108,776,473 (GRCm39)	N298D	probably benign	Het

Other mutations in Muc21

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
BB009:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
FR4304:Muc21	UTSW	17	35,933,013 (GRCm39)	intron	probably benign
R0334:Muc21	UTSW	17	35,933,614 (GRCm39)	intron	probably benign
R0946:Muc21	UTSW	17	35,929,105 (GRCm39)	missense	probably benign	0.32
R1117:Muc21	UTSW	17	35,930,920 (GRCm39)	intron	probably benign
R1345:Muc21	UTSW	17	35,932,489 (GRCm39)	intron	probably benign
R1697:Muc21	UTSW	17	35,931,540 (GRCm39)	intron	probably benign
R1750:Muc21	UTSW	17	35,931,940 (GRCm39)	intron	probably benign
R1756:Muc21	UTSW	17	35,930,131 (GRCm39)	intron	probably benign
R1946:Muc21	UTSW	17	35,933,416 (GRCm39)	intron	probably benign
R1978:Muc21	UTSW	17	35,933,857 (GRCm39)	intron	probably benign
R1991:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R1992:Muc21	UTSW	17	35,929,600 (GRCm39)	missense	probably benign	0.32
R2063:Muc21	UTSW	17	35,932,297 (GRCm39)	intron	probably benign
R2356:Muc21	UTSW	17	35,932,563 (GRCm39)	intron	probably benign
R2866:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R3826:Muc21	UTSW	17	35,932,504 (GRCm39)	intron	probably benign
R4020:Muc21	UTSW	17	35,930,953 (GRCm39)	intron	probably benign
R4474:Muc21	UTSW	17	35,931,496 (GRCm39)	intron	probably benign
R4677:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R4786:Muc21	UTSW	17	35,930,221 (GRCm39)	intron	probably benign
R5071:Muc21	UTSW	17	35,931,444 (GRCm39)	intron	probably benign
R5173:Muc21	UTSW	17	35,931,633 (GRCm39)	intron	probably benign
R5283:Muc21	UTSW	17	35,932,224 (GRCm39)	intron	probably benign
R5446:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5542:Muc21	UTSW	17	35,933,395 (GRCm39)	intron	probably benign
R5913:Muc21	UTSW	17	35,934,123 (GRCm39)	intron	probably benign
R6011:Muc21	UTSW	17	35,933,074 (GRCm39)	intron	probably benign
R6198:Muc21	UTSW	17	35,931,808 (GRCm39)	intron	probably benign
R6394:Muc21	UTSW	17	35,931,058 (GRCm39)	intron	probably benign
R6786:Muc21	UTSW	17	35,934,057 (GRCm39)	intron	probably benign
R6940:Muc21	UTSW	17	35,934,118 (GRCm39)	intron	probably benign
R7082:Muc21	UTSW	17	35,932,093 (GRCm39)	missense	unknown
R7103:Muc21	UTSW	17	35,932,432 (GRCm39)	missense	unknown
R7110:Muc21	UTSW	17	35,933,510 (GRCm39)	intron	probably benign
R7139:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7165:Muc21	UTSW	17	35,932,870 (GRCm39)	missense	unknown
R7200:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R7204:Muc21	UTSW	17	35,932,105 (GRCm39)	intron	probably benign
R7289:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7290:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7295:Muc21	UTSW	17	35,929,761 (GRCm39)	missense	unknown
R7319:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R7462:Muc21	UTSW	17	35,931,568 (GRCm39)	missense	unknown
R7529:Muc21	UTSW	17	35,930,123 (GRCm39)	missense	unknown
R7718:Muc21	UTSW	17	35,933,728 (GRCm39)	missense	unknown
R7762:Muc21	UTSW	17	35,932,977 (GRCm39)	missense	unknown
R7788:Muc21	UTSW	17	35,929,798 (GRCm39)	missense	unknown
R7798:Muc21	UTSW	17	35,932,146 (GRCm39)	missense	unknown
R7831:Muc21	UTSW	17	35,929,651 (GRCm39)	missense	unknown
R7896:Muc21	UTSW	17	35,930,917 (GRCm39)	missense	unknown
R7899:Muc21	UTSW	17	35,931,493 (GRCm39)	intron	probably benign
R7932:Muc21	UTSW	17	35,933,525 (GRCm39)	intron	probably benign
R8025:Muc21	UTSW	17	35,931,879 (GRCm39)	intron	probably benign
R8077:Muc21	UTSW	17	35,930,628 (GRCm39)	intron	probably benign
R8090:Muc21	UTSW	17	35,932,617 (GRCm39)	missense	unknown
R8169:Muc21	UTSW	17	35,932,072 (GRCm39)	missense	unknown
R8184:Muc21	UTSW	17	35,933,722 (GRCm39)	missense	unknown
R8209:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8226:Muc21	UTSW	17	35,930,599 (GRCm39)	intron	probably benign
R8464:Muc21	UTSW	17	35,933,098 (GRCm39)	intron	probably benign
R8670:Muc21	UTSW	17	35,932,540 (GRCm39)	missense	unknown
R8783:Muc21	UTSW	17	35,930,875 (GRCm39)	missense	unknown
R8856:Muc21	UTSW	17	35,931,865 (GRCm39)	missense	unknown
R9155:Muc21	UTSW	17	35,932,131 (GRCm39)	missense	unknown
R9214:Muc21	UTSW	17	35,931,838 (GRCm39)	missense	unknown
R9353:Muc21	UTSW	17	35,930,545 (GRCm39)	missense	unknown
R9618:Muc21	UTSW	17	35,932,935 (GRCm39)	intron	probably benign
R9621:Muc21	UTSW	17	35,932,720 (GRCm39)	missense	unknown
R9679:Muc21	UTSW	17	35,930,491 (GRCm39)	missense	unknown
RF025:Muc21	UTSW	17	35,933,771 (GRCm39)	intron	probably benign
Z1176:Muc21	UTSW	17	35,932,137 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,951 (GRCm39)	missense	unknown
Z1177:Muc21	UTSW	17	35,931,817 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TAGATCCTGAGGCAGAGCTG -3'
(R):5'- TGAATCCAGCACTGCCTCAG -3'

Sequencing Primer
(F):5'- CCTGAGGCAGTGCTGGATTC -3'
(R):5'- TCAGGCTCTACACCTACCCGG -3'

Posted On

2017-01-03