Mutagenetix > Incidental Mutation

Incidental Mutation 'R5719:Zranb3'

451326

Institutional Source

Beutler Lab

Gene Symbol

Zranb3

Ensembl Gene

ENSMUSG00000036086

Gene Name

zinc finger, RAN-binding domain containing 3

Synonyms

4933425L19Rik

MMRRC Submission

043339-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.146) question?

Stock #

R5719 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

127881921-128030784 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 127891613 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Glycine at position 788 (S788G)

Ref Sequence

ENSEMBL: ENSMUSP00000108157 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086614] [ENSMUST00000112538]

AlphaFold

Q6NZP1

Predicted Effect

probably benign
Transcript: ENSMUST00000086614
AA Change: S788G

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000083806
Gene: ENSMUSG00000036086
AA Change: S788G

Domain	Start	End	E-Value	Type
DEXDc	33	214	3.37e-19	SMART
HELICc	352	435	3.79e-13	SMART
ZnF_RBZ	619	643	6.93e-5	SMART
HNHc	985	1036	5.64e-3	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000112538
AA Change: S788G

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000108157
Gene: ENSMUSG00000036086
AA Change: S788G

Domain	Start	End	E-Value	Type
Pfam:SNF2_N	40	98	6.6e-12	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000143389

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000186230

Meta Mutation Damage Score

0.0599

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.3%

Validation Efficiency

98% (79/81)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
A130051J06Rik	C	T	15: 95,688,641 (GRCm39)		probably benign	Het
A630001G21Rik	T	A	1: 85,651,106 (GRCm39)	R110W	probably benign	Het
Abca4	T	C	3: 121,928,915 (GRCm39)		probably null	Het
Abcc3	T	C	11: 94,241,894 (GRCm39)	N1379S	probably damaging	Het
Actrt3	A	C	3: 30,652,276 (GRCm39)	F273V	probably benign	Het
Adam22	T	C	5: 8,417,217 (GRCm39)	D75G	probably benign	Het
Ash1l	C	A	3: 88,961,805 (GRCm39)	D2392E	possibly damaging	Het
Ash1l	T	C	3: 88,965,933 (GRCm39)	I2445T	probably damaging	Het
Bltp2	T	A	11: 78,164,071 (GRCm39)	H1137Q	probably damaging	Het
Cacna2d2	A	G	9: 107,401,851 (GRCm39)	I762V	probably benign	Het
Ccdc127	T	A	13: 74,505,187 (GRCm39)		probably benign	Het
Ccdc91	C	G	6: 147,477,001 (GRCm39)	L230V	unknown	Het
Cdk13	A	G	13: 17,894,240 (GRCm39)	I1129T	probably damaging	Het
Cnot1	C	T	8: 96,470,924 (GRCm39)	R1308H	possibly damaging	Het
Crhr2	T	C	6: 55,080,207 (GRCm39)	H144R	probably damaging	Het
Dnmt1	T	C	9: 20,823,891 (GRCm39)	N993S	possibly damaging	Het
Eif4g1	T	A	16: 20,507,761 (GRCm39)	V1182D	probably damaging	Het
Eml2	G	A	7: 18,935,088 (GRCm39)	V432I	probably damaging	Het
Fam234a	T	A	17: 26,433,627 (GRCm39)	Q399L	possibly damaging	Het
Fyb1	A	T	15: 6,610,350 (GRCm39)	K308*	probably null	Het
Gfral	C	T	9: 76,104,328 (GRCm39)	R228Q	probably benign	Het
Gm10309	A	T	17: 86,806,421 (GRCm39)		probably benign	Het
Gm11595	G	A	11: 99,663,381 (GRCm39)	R100C	unknown	Het
Gm5501	G	A	18: 9,917,417 (GRCm39)		noncoding transcript	Het
Gm6309	A	T	5: 146,104,992 (GRCm39)	V307D	probably benign	Het
Gm9871	T	A	6: 101,773,148 (GRCm39)		noncoding transcript	Het
Greb1l	G	A	18: 10,542,427 (GRCm39)	E1341K	probably damaging	Het
Herc3	T	A	6: 58,871,528 (GRCm39)	V70E	possibly damaging	Het
Hes7	A	G	11: 69,012,415 (GRCm39)	E41G	probably damaging	Het
Ifi27l2b	T	C	12: 103,422,046 (GRCm39)	D106G	unknown	Het
Igfbp6	A	T	15: 102,056,616 (GRCm39)	Y184F	probably damaging	Het
Isyna1	A	G	8: 71,047,352 (GRCm39)	Y25C	probably damaging	Het
Kcng3	G	T	17: 83,938,563 (GRCm39)	T162K	possibly damaging	Het
Krt36	T	C	11: 99,994,987 (GRCm39)	D195G	possibly damaging	Het
Lrwd1	A	T	5: 136,161,093 (GRCm39)		probably null	Het
Lsg1	C	T	16: 30,380,593 (GRCm39)	A615T	probably benign	Het
Myo5a	A	T	9: 75,059,213 (GRCm39)	E480D	probably damaging	Het
Myrf	T	A	19: 10,194,087 (GRCm39)	D690V	probably damaging	Het
N4bp1	T	C	8: 87,578,312 (GRCm39)	I684M	probably damaging	Het
Nlrc3	C	T	16: 3,781,589 (GRCm39)	A607T	probably damaging	Het
Nuak1	A	T	10: 84,245,584 (GRCm39)	I87N	probably damaging	Het
Odad2	G	T	18: 7,211,496 (GRCm39)	Q793K	probably benign	Het
Or5p66	T	G	7: 107,885,599 (GRCm39)	T245P	probably damaging	Het
Or5w11	T	C	2: 87,459,475 (GRCm39)		probably null	Het
Or8b4	A	T	9: 37,830,647 (GRCm39)	E236D	probably damaging	Het
Osbpl9	T	A	4: 108,919,763 (GRCm39)	R689*	probably null	Het
Ppargc1b	T	A	18: 61,440,639 (GRCm39)	M744L	probably benign	Het
Prss40	A	T	1: 34,591,598 (GRCm39)		probably benign	Het
Ptprd	T	A	4: 75,972,839 (GRCm39)		probably null	Het
Rft1	T	C	14: 30,385,183 (GRCm39)		probably benign	Het
Rftn2	C	T	1: 55,253,445 (GRCm39)	V53I	probably damaging	Het
Rnaset2a	T	C	17: 8,350,879 (GRCm39)	Y167C	probably damaging	Het
Schip1	T	C	3: 68,315,560 (GRCm39)		probably benign	Het
Scn5a	A	C	9: 119,359,118 (GRCm39)	L643R	possibly damaging	Het
Shroom3	T	A	5: 93,090,877 (GRCm39)	M1128K	probably benign	Het
Skint8	C	A	4: 111,807,390 (GRCm39)	L359M	probably damaging	Het
Slc14a2	A	G	18: 78,252,257 (GRCm39)	L18P	probably benign	Het
Slc22a3	C	T	17: 12,642,691 (GRCm39)	V509M	probably damaging	Het
Slc7a5	A	C	8: 122,610,381 (GRCm39)	F478V	probably benign	Het
Smc1b	T	A	15: 84,980,859 (GRCm39)	N803I	probably benign	Het
Snf8	T	A	11: 95,932,551 (GRCm39)	N115K	probably damaging	Het
Stox2	T	A	8: 47,866,172 (GRCm39)	K57*	probably null	Het
Tmem248	T	A	5: 130,258,429 (GRCm39)	F41I	probably damaging	Het
Tmprss7	T	C	16: 45,506,793 (GRCm39)	S90G	probably damaging	Het
Top3b	T	C	16: 16,703,700 (GRCm39)	V285A	probably damaging	Het
Tsen15	T	C	1: 152,247,534 (GRCm39)	T153A	probably damaging	Het
Usp34	T	G	11: 23,304,846 (GRCm39)	S360A	probably benign	Het
Wdr24	T	C	17: 26,047,314 (GRCm39)		probably null	Het
Zbtb2	G	A	10: 4,319,456 (GRCm39)	T190I	probably benign	Het

Other mutations in Zranb3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00565:Zranb3	APN	1	127,943,877 (GRCm39)	missense	probably benign	0.01
IGL00818:Zranb3	APN	1	127,960,604 (GRCm39)	missense	probably damaging	1.00
IGL01360:Zranb3	APN	1	127,887,622 (GRCm39)	nonsense	probably null
IGL01704:Zranb3	APN	1	127,895,676 (GRCm39)	missense	possibly damaging	0.93
IGL02131:Zranb3	APN	1	127,920,688 (GRCm39)	missense	probably damaging	1.00
IGL02466:Zranb3	APN	1	127,943,829 (GRCm39)	missense	probably benign	0.08
IGL02825:Zranb3	APN	1	127,887,489 (GRCm39)	missense	probably benign	0.13
IGL02836:Zranb3	APN	1	127,888,562 (GRCm39)	missense	probably benign	0.00
R0088:Zranb3	UTSW	1	127,904,199 (GRCm39)	missense	probably benign
R0279:Zranb3	UTSW	1	127,891,510 (GRCm39)	missense	probably benign	0.01
R0423:Zranb3	UTSW	1	128,019,607 (GRCm39)	missense	probably damaging	1.00
R0499:Zranb3	UTSW	1	127,882,817 (GRCm39)	splice site	probably null
R0562:Zranb3	UTSW	1	127,964,295 (GRCm39)	missense	probably benign	0.04
R0972:Zranb3	UTSW	1	127,884,383 (GRCm39)	missense	probably damaging	1.00
R1480:Zranb3	UTSW	1	128,019,599 (GRCm39)	missense	probably damaging	1.00
R1552:Zranb3	UTSW	1	127,888,488 (GRCm39)	splice site	probably benign
R1704:Zranb3	UTSW	1	128,019,740 (GRCm39)	start codon destroyed	probably null	0.22
R1817:Zranb3	UTSW	1	127,945,293 (GRCm39)	critical splice donor site	probably null
R1818:Zranb3	UTSW	1	127,945,293 (GRCm39)	critical splice donor site	probably null
R1819:Zranb3	UTSW	1	127,945,293 (GRCm39)	critical splice donor site	probably null
R1951:Zranb3	UTSW	1	127,927,136 (GRCm39)	missense	probably damaging	1.00
R1953:Zranb3	UTSW	1	127,927,136 (GRCm39)	missense	probably damaging	1.00
R1988:Zranb3	UTSW	1	127,887,480 (GRCm39)	missense	probably benign
R2011:Zranb3	UTSW	1	128,019,638 (GRCm39)	missense	probably benign	0.00
R3159:Zranb3	UTSW	1	127,900,686 (GRCm39)	missense	probably benign
R4179:Zranb3	UTSW	1	127,888,601 (GRCm39)	missense	possibly damaging	0.88
R4281:Zranb3	UTSW	1	127,891,614 (GRCm39)	missense	possibly damaging	0.69
R4400:Zranb3	UTSW	1	127,884,392 (GRCm39)	missense	possibly damaging	0.87
R5236:Zranb3	UTSW	1	127,968,726 (GRCm39)	missense	probably damaging	1.00
R5330:Zranb3	UTSW	1	127,887,457 (GRCm39)	missense	probably damaging	0.99
R6125:Zranb3	UTSW	1	127,887,482 (GRCm39)	missense	probably benign
R6220:Zranb3	UTSW	1	127,927,141 (GRCm39)	missense	probably benign	0.44
R6414:Zranb3	UTSW	1	127,968,694 (GRCm39)	missense	probably benign	0.08
R6751:Zranb3	UTSW	1	127,887,556 (GRCm39)	missense	probably benign
R7229:Zranb3	UTSW	1	127,968,630 (GRCm39)	missense	probably benign	0.00
R7419:Zranb3	UTSW	1	127,891,588 (GRCm39)	missense	possibly damaging	0.86
R7537:Zranb3	UTSW	1	127,960,584 (GRCm39)	critical splice donor site	probably null
R7771:Zranb3	UTSW	1	127,960,605 (GRCm39)	missense	probably damaging	1.00
R7980:Zranb3	UTSW	1	128,030,671 (GRCm39)	unclassified	probably benign
R8152:Zranb3	UTSW	1	127,882,732 (GRCm39)	missense	probably damaging	1.00
R8370:Zranb3	UTSW	1	127,895,670 (GRCm39)	missense	probably benign	0.00
R8458:Zranb3	UTSW	1	127,920,647 (GRCm39)	missense	probably damaging	1.00
R8816:Zranb3	UTSW	1	127,964,347 (GRCm39)	missense	possibly damaging	0.95
R8969:Zranb3	UTSW	1	127,888,588 (GRCm39)	missense	possibly damaging	0.80
R9369:Zranb3	UTSW	1	127,887,828 (GRCm39)	missense	probably benign	0.00
R9468:Zranb3	UTSW	1	127,891,496 (GRCm39)	critical splice donor site	probably null
Z1176:Zranb3	UTSW	1	127,964,218 (GRCm39)	missense	probably benign	0.25
Z1176:Zranb3	UTSW	1	127,892,885 (GRCm39)	missense	possibly damaging	0.55

Predicted Primers

PCR Primer
(F):5'- GCCTCAGATCTATAGAAACGGC -3'
(R):5'- GCACTCTGGATTGAGATGGAC -3'

Sequencing Primer
(F):5'- TCAGATCTATAGAAACGGCCCTGG -3'
(R):5'- CACTCTGGATTGAGATGGACATAGG -3'

Posted On

2017-01-03