Mutagenetix > Incidental Mutation

Incidental Mutation 'R5733:Mucl3'

451558

Institutional Source

Beutler Lab

Gene Symbol

Mucl3

Ensembl Gene

ENSMUSG00000073408

Gene Name

mucin like 3

Synonyms

LOC268949, Dpcr1

MMRRC Submission

043193-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.052) question?

Stock #

R5733 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

35946647-35954587 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 35949102 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Valine at position 166 (M166V)

Ref Sequence

ENSEMBL: ENSMUSP00000093120 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000095467] [ENSMUST00000174521]

AlphaFold

Q3TNW5

Predicted Effect

probably benign
Transcript: ENSMUST00000095467
AA Change: M166V

PolyPhen 2 Score 0.004 (Sensitivity: 0.98; Specificity: 0.59)

SMART Domains

Protein: ENSMUSP00000093120
Gene: ENSMUSG00000073408
AA Change: M166V

Domain	Start	End	E-Value	Type
signal peptide	1	27	N/A	INTRINSIC
transmembrane domain	428	450	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000174521

SMART Domains

Protein: ENSMUSP00000134221
Gene: ENSMUSG00000090509

Domain	Start	End	E-Value	Type
Pfam:SFTA2	80	117	9.2e-18	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000174534

Coding Region Coverage

1x: 99.1%
3x: 98.4%
10x: 97.0%
20x: 95.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 43 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acox3	T	A	5: 35,762,543 (GRCm39)		probably null	Het
Ahnak2	A	T	12: 112,742,100 (GRCm39)	Y657*	probably null	Het
Anxa3	T	A	5: 96,968,331 (GRCm39)	I128N	probably damaging	Het
Bsnd	T	C	4: 106,345,198 (GRCm39)	T83A	probably benign	Het
Capn10	A	G	1: 92,871,635 (GRCm39)	Y411C	probably benign	Het
Capn3	G	A	2: 120,315,075 (GRCm39)	W201*	probably null	Het
Crtap	T	C	9: 114,207,164 (GRCm39)	T365A	probably benign	Het
Daam1	A	G	12: 71,992,272 (GRCm39)	D329G	unknown	Het
Dmxl2	A	T	9: 54,283,550 (GRCm39)	L2761Q	possibly damaging	Het
Fcho2	A	C	13: 98,926,310 (GRCm39)	V91G	probably damaging	Het
Fen1	A	T	19: 10,178,022 (GRCm39)	C141S	possibly damaging	Het
Fkbp15	G	C	4: 62,225,166 (GRCm39)	A831G	probably benign	Het
Frmd4a	A	T	2: 4,305,768 (GRCm39)	R14S	possibly damaging	Het
Fzr1	A	G	10: 81,206,160 (GRCm39)	F176L	possibly damaging	Het
Garem2	A	G	5: 30,321,336 (GRCm39)	D565G	probably damaging	Het
Garre1	A	T	7: 33,944,505 (GRCm39)	S76T	probably damaging	Het
Iqca1	G	T	1: 89,998,257 (GRCm39)	T549K	probably damaging	Het
Itgax	T	A	7: 127,739,647 (GRCm39)	S686R	probably damaging	Het
Knop1	C	A	7: 118,445,305 (GRCm39)	G220C	probably damaging	Het
Lyzl1	T	A	18: 4,169,142 (GRCm39)	C49S	probably damaging	Het
Mpzl1	A	T	1: 165,433,180 (GRCm39)	I157K	probably benign	Het
Mrgprb2	T	C	7: 48,202,261 (GRCm39)	I155V	probably benign	Het
Mvb12b	T	C	2: 33,717,728 (GRCm39)	T167A	probably benign	Het
Myh3	A	G	11: 66,979,445 (GRCm39)	N491S	probably benign	Het
Myo5b	A	G	18: 74,787,128 (GRCm39)	D511G	possibly damaging	Het
Or10ak8	C	T	4: 118,774,035 (GRCm39)	V210I	probably benign	Het
Or11h4	T	C	14: 50,974,509 (GRCm39)	T37A	probably benign	Het
Or6b1	C	T	6: 42,815,180 (GRCm39)	R122C	probably damaging	Het
Or8k21	T	G	2: 86,145,558 (GRCm39)	Q24P	probably damaging	Het
Ptcd1	A	G	5: 145,091,671 (GRCm39)	M476T	probably damaging	Het
Pum3	A	G	19: 27,398,695 (GRCm39)		probably null	Het
Ranbp2	G	A	10: 58,321,658 (GRCm39)	D2652N	probably damaging	Het
Rassf1	T	C	9: 107,435,213 (GRCm39)	V166A	probably damaging	Het
Rictor	A	G	15: 6,812,585 (GRCm39)	H907R	probably benign	Het
Rorb	T	C	19: 18,965,471 (GRCm39)	E6G	probably damaging	Het
Serpina3f	A	T	12: 104,183,182 (GRCm39)	T15S	possibly damaging	Het
Sorbs2	T	C	8: 46,212,226 (GRCm39)	L100P	probably damaging	Het
Sprr2k	T	C	3: 92,340,655 (GRCm39)		probably benign	Het
Srrm2	T	A	17: 24,040,360 (GRCm39)	S2431T	probably damaging	Het
Stox2	T	A	8: 47,866,172 (GRCm39)	K57*	probably null	Het
Ttc21a	G	A	9: 119,770,327 (GRCm39)	V133I	probably benign	Het
Vasn	T	C	16: 4,468,026 (GRCm39)	Y658H	possibly damaging	Het
Zfp251	A	G	15: 76,754,527 (GRCm39)	Y35H	probably damaging	Het

Other mutations in Mucl3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00476:Mucl3	APN	17	35,948,994 (GRCm39)	missense	possibly damaging	0.46
IGL01321:Mucl3	APN	17	35,947,758 (GRCm39)	missense	probably damaging	0.98
IGL01805:Mucl3	APN	17	35,948,535 (GRCm39)	missense	possibly damaging	0.62
IGL02320:Mucl3	APN	17	35,948,332 (GRCm39)	missense	probably benign	0.00
R1869:Mucl3	UTSW	17	35,949,305 (GRCm39)	missense	possibly damaging	0.82
R4570:Mucl3	UTSW	17	35,948,883 (GRCm39)	missense	possibly damaging	0.66
R4858:Mucl3	UTSW	17	35,948,468 (GRCm39)	missense	possibly damaging	0.74
R5592:Mucl3	UTSW	17	35,954,535 (GRCm39)	missense	probably damaging	0.99
R7422:Mucl3	UTSW	17	35,949,312 (GRCm39)	missense	probably benign	0.00
R7689:Mucl3	UTSW	17	35,948,969 (GRCm39)	missense	possibly damaging	0.83
R7780:Mucl3	UTSW	17	35,947,874 (GRCm39)	missense	possibly damaging	0.95
R8079:Mucl3	UTSW	17	35,949,084 (GRCm39)	missense	unknown
R8320:Mucl3	UTSW	17	35,954,530 (GRCm39)	missense	probably benign	0.22
R8337:Mucl3	UTSW	17	35,948,486 (GRCm39)	missense	possibly damaging	0.95
R8867:Mucl3	UTSW	17	35,948,872 (GRCm39)	missense	probably benign	0.27
R9734:Mucl3	UTSW	17	35,949,233 (GRCm39)	missense	probably benign	0.07

Predicted Primers

PCR Primer
(F):5'- GCAAACGGAATTGTGTGATGTTC -3'
(R):5'- CACTGTCCGTCATGAAATGCC -3'

Sequencing Primer
(F):5'- AAACGGAATTGTGTGATGTTCAGGTC -3'
(R):5'- CTCCGGCTTCAGAGAAGGATC -3'

Posted On

2017-01-03