Mutagenetix > Incidental Mutation

Incidental Mutation 'R5735:Or8h10'

451630

Institutional Source

Beutler Lab

Gene Symbol

Or8h10

Ensembl Gene

ENSMUSG00000070875

Gene Name

olfactory receptor family 8 subfamily H member 10

Synonyms

Olfr1100, GA_x6K02T2Q125-48465387-48464422, MOR206-4

MMRRC Submission

043349-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.101) question?

Stock #

R5735 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86808173-86809138 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 86809044 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 32 (V32A)

Ref Sequence

ENSEMBL: ENSMUSP00000151088 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094913] [ENSMUST00000117211]

AlphaFold

Q8VFM1

Predicted Effect

probably benign
Transcript: ENSMUST00000094913
AA Change: V32A

PolyPhen 2 Score 0.075 (Sensitivity: 0.93; Specificity: 0.85)

SMART Domains

Protein: ENSMUSP00000092517
Gene: ENSMUSG00000070875
AA Change: V32A

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	1.7e-51	PFAM
Pfam:7tm_1	41	314	2.3e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000117211
AA Change: V32A

PolyPhen 2 Score 0.075 (Sensitivity: 0.93; Specificity: 0.85)

Coding Region Coverage

1x: 99.0%
3x: 98.3%
10x: 96.8%
20x: 94.4%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 67 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts16	C	A	13: 70,984,337 (GRCm39)	D109Y	possibly damaging	Het
Armc8	T	A	9: 99,379,447 (GRCm39)		probably null	Het
Atg4b	G	T	1: 93,701,519 (GRCm39)	G71V	probably damaging	Het
Atp10b	A	T	11: 43,042,601 (GRCm39)	M54L	probably benign	Het
Baiap2l1	A	G	5: 144,223,112 (GRCm39)	L75P	probably damaging	Het
Bnc2	A	C	4: 84,210,908 (GRCm39)	L487R	probably damaging	Het
Cacng7	T	C	7: 3,387,539 (GRCm39)	S141P	probably benign	Het
Carmil2	G	A	8: 106,424,663 (GRCm39)	G1361D	probably damaging	Het
Cenpf	T	C	1: 189,386,560 (GRCm39)	I1907V	probably benign	Het
Cep192	T	C	18: 68,013,866 (GRCm39)	V2422A	probably benign	Het
Cfap73	T	A	5: 120,770,671 (GRCm39)	Q33L	probably benign	Het
Cip2a	T	A	16: 48,837,856 (GRCm39)		probably null	Het
Cmtm2a	A	T	8: 105,019,418 (GRCm39)	I57N	probably damaging	Het
Col6a2	T	A	10: 76,435,727 (GRCm39)	D836V	probably benign	Het
Dnah2	A	G	11: 69,321,643 (GRCm39)	V3839A	possibly damaging	Het
Enpp1	A	T	10: 24,530,817 (GRCm39)	F546L	possibly damaging	Het
Eps8l2	A	G	7: 140,940,290 (GRCm39)	T507A	probably damaging	Het
Erg28	C	T	12: 85,862,926 (GRCm39)	E129K	probably benign	Het
Erlec1	A	T	11: 30,900,591 (GRCm39)	N153K	probably benign	Het
Fam234a	T	C	17: 26,432,679 (GRCm39)	E490G	probably damaging	Het
Fat4	C	T	3: 39,003,725 (GRCm39)	R1815C	probably damaging	Het
Galnt1	T	G	18: 24,397,577 (GRCm39)	I226S	possibly damaging	Het
Ifnl2	T	A	7: 28,209,614 (GRCm39)	I58F	possibly damaging	Het
Itih5	A	G	2: 10,245,572 (GRCm39)	N554D	probably benign	Het
Kcna10	A	G	3: 107,102,394 (GRCm39)	I342V	probably benign	Het
Kif6	G	A	17: 50,139,210 (GRCm39)	E561K	probably damaging	Het
Kl	A	G	5: 150,915,003 (GRCm39)	N910S	possibly damaging	Het
Lpar2	G	T	8: 70,276,385 (GRCm39)	R58L	probably damaging	Het
Macrod2	A	G	2: 140,260,809 (GRCm39)	T27A	possibly damaging	Het
Mfsd2a	A	T	4: 122,843,120 (GRCm39)	V387D	probably damaging	Het
Npas3	A	T	12: 54,050,262 (GRCm39)	T231S	probably benign	Het
Or4a70	A	T	2: 89,323,812 (GRCm39)	N281K	probably damaging	Het
Or52n5	A	T	7: 104,587,966 (GRCm39)	T78S	probably benign	Het
Or5aq1b	A	T	2: 86,901,756 (GRCm39)	C241S	probably damaging	Het
Otx1	C	A	11: 21,947,037 (GRCm39)	A91S	probably damaging	Het
Pde10a	A	T	17: 9,160,024 (GRCm39)	I432F	probably damaging	Het
Phka2	ACC	AC	X: 159,342,862 (GRCm39)		probably null	Het
Pomt1	G	A	2: 32,133,517 (GRCm39)	G218R	probably damaging	Het
Potefam1	A	T	2: 111,055,837 (GRCm39)	L183*	probably null	Het
Pramel22	A	T	4: 143,381,205 (GRCm39)	C273S	probably damaging	Het
Prdm5	C	T	6: 65,904,974 (GRCm39)	T157I	possibly damaging	Het
Psen2	T	A	1: 180,068,491 (GRCm39)	E54V	probably benign	Het
Ptpn13	C	A	5: 103,702,686 (GRCm39)	H1217Q	probably benign	Het
Ptprt	A	G	2: 161,376,484 (GRCm39)	S1306P	probably damaging	Het
Ptpru	G	T	4: 131,565,401 (GRCm39)	P23T	probably benign	Het
Rtn3	T	C	19: 7,434,057 (GRCm39)	E626G	probably damaging	Het
Scn3a	A	T	2: 65,312,622 (GRCm39)	M1191K	probably damaging	Het
Scn3a	T	A	2: 65,314,803 (GRCm39)	N1086I	probably benign	Het
Sgms2	A	T	3: 131,129,866 (GRCm39)	M174K	probably damaging	Het
Skor1	T	A	9: 63,053,346 (GRCm39)	I180F	probably damaging	Het
Slit2	T	G	5: 48,416,958 (GRCm39)	C1003W	probably damaging	Het
Tbc1d2b	T	A	9: 90,104,462 (GRCm39)	Q560L	possibly damaging	Het
Themis	A	G	10: 28,598,530 (GRCm39)	I51V	probably benign	Het
Tmem203	T	C	2: 25,146,085 (GRCm39)	V135A	probably benign	Het
Tns1	T	C	1: 73,967,138 (GRCm39)	T1212A	probably benign	Het
Trgv5	T	G	13: 19,376,695 (GRCm39)	H47Q	probably benign	Het
Trim2	G	A	3: 84,075,029 (GRCm39)	A697V	probably damaging	Het
Ubxn4	T	C	1: 128,186,677 (GRCm39)	S37P	possibly damaging	Het
Vmn2r17	A	G	5: 109,600,716 (GRCm39)	I671M	possibly damaging	Het
Vmn2r94	G	T	17: 18,464,066 (GRCm39)	S741R	probably damaging	Het
Vwce	C	A	19: 10,624,431 (GRCm39)	D414E	probably benign	Het
Zfp397	T	C	18: 24,093,249 (GRCm39)	S245P	possibly damaging	Het
Zfp747l1	G	T	7: 126,984,579 (GRCm39)	H174Q	possibly damaging	Het
Zfp809	T	A	9: 22,150,227 (GRCm39)	Y241*	probably null	Het
Zfp995	C	T	17: 22,101,010 (GRCm39)	C29Y	probably benign	Het
Zfta	A	G	19: 7,400,161 (GRCm39)	E209G	probably benign	Het
Zfyve26	T	C	12: 79,320,147 (GRCm39)	D1066G	probably damaging	Het

Other mutations in Or8h10

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02387:Or8h10	APN	2	86,808,193 (GRCm39)	nonsense	probably null
R0102:Or8h10	UTSW	2	86,808,549 (GRCm39)	missense	possibly damaging	0.90
R0477:Or8h10	UTSW	2	86,808,567 (GRCm39)	missense	probably damaging	0.99
R0586:Or8h10	UTSW	2	86,809,126 (GRCm39)	missense	probably damaging	0.99
R0743:Or8h10	UTSW	2	86,808,843 (GRCm39)	missense	probably benign	0.12
R1163:Or8h10	UTSW	2	86,809,020 (GRCm39)	missense	probably damaging	1.00
R1640:Or8h10	UTSW	2	86,808,963 (GRCm39)	missense	probably damaging	1.00
R2865:Or8h10	UTSW	2	86,808,805 (GRCm39)	missense	possibly damaging	0.56
R4478:Or8h10	UTSW	2	86,808,562 (GRCm39)	missense	probably benign
R4548:Or8h10	UTSW	2	86,809,014 (GRCm39)	missense	probably damaging	0.98
R4618:Or8h10	UTSW	2	86,808,618 (GRCm39)	missense	possibly damaging	0.87
R4829:Or8h10	UTSW	2	86,808,918 (GRCm39)	missense	probably damaging	0.99
R4858:Or8h10	UTSW	2	86,808,693 (GRCm39)	missense	probably damaging	1.00
R5071:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5072:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5073:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5074:Or8h10	UTSW	2	86,808,666 (GRCm39)	missense	possibly damaging	0.88
R5574:Or8h10	UTSW	2	86,808,867 (GRCm39)	missense	probably benign	0.00
R5874:Or8h10	UTSW	2	86,808,786 (GRCm39)	missense	probably damaging	0.99
R6228:Or8h10	UTSW	2	86,809,035 (GRCm39)	missense	probably damaging	1.00
R6689:Or8h10	UTSW	2	86,808,498 (GRCm39)	missense	probably benign	0.36
R6979:Or8h10	UTSW	2	86,808,577 (GRCm39)	missense	probably damaging	1.00
R7006:Or8h10	UTSW	2	86,808,303 (GRCm39)	missense	probably damaging	0.99
R7274:Or8h10	UTSW	2	86,808,867 (GRCm39)	missense	probably benign	0.00
R7399:Or8h10	UTSW	2	86,808,501 (GRCm39)	missense	probably benign	0.19
R7560:Or8h10	UTSW	2	86,809,122 (GRCm39)	missense	probably benign	0.00
R7765:Or8h10	UTSW	2	86,808,538 (GRCm39)	missense	probably damaging	0.98
R8381:Or8h10	UTSW	2	86,808,373 (GRCm39)	missense	probably benign	0.36
R8390:Or8h10	UTSW	2	86,808,501 (GRCm39)	missense	probably benign	0.19
R8694:Or8h10	UTSW	2	86,808,591 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TGGAAGTCAGCAGATTCTGTAAAG -3'
(R):5'- GGCATGTGTGTCAGTAGTACC -3'

Sequencing Primer
(F):5'- CAGCAGATTCTGTAAAGTTTTAGGTG -3'
(R):5'- CATGTGTGTCAGTAGTACCTATAGAC -3'

Posted On

2017-01-03