Mutagenetix > Incidental Mutation

Incidental Mutation 'R5706:Vmn2r109'

451994

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r109

Ensembl Gene

ENSMUSG00000090572

Gene Name

vomeronasal 2, receptor 109

Synonyms

EG627814

MMRRC Submission

043331-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.069) question?

Stock #

R5706 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

20760779-20785018 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 20774567 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 263 (W263R)

Ref Sequence

ENSEMBL: ENSMUSP00000132641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167093]

AlphaFold

K7N747

Predicted Effect

probably benign
Transcript: ENSMUST00000167093
AA Change: W263R

PolyPhen 2 Score 0.160 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000132641
Gene: ENSMUSG00000090572
AA Change: W263R

Domain	Start	End	E-Value	Type
signal peptide	1	19	N/A	INTRINSIC
Pfam:ANF_receptor	83	467	1.4e-35	PFAM
Pfam:NCD3G	510	563	3.1e-21	PFAM
Pfam:7tm_3	596	831	7.4e-52	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 95.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 41 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca4	T	A	3: 121,847,910 (GRCm39)	M77K	probably benign	Het
Amy1	A	G	3: 113,349,769 (GRCm39)	V467A	probably damaging	Het
Atf4	T	C	15: 80,140,531 (GRCm39)	V11A	possibly damaging	Het
B4galt1	T	C	4: 40,807,268 (GRCm39)	N373D	probably damaging	Het
Bpifa3	A	G	2: 153,977,498 (GRCm39)	K112R	probably damaging	Het
Chd2	T	A	7: 73,141,105 (GRCm39)	Y596F	possibly damaging	Het
Clspn	T	C	4: 126,472,211 (GRCm39)	S962P	probably damaging	Het
Cyp2c68	T	C	19: 39,722,762 (GRCm39)	D262G	possibly damaging	Het
Dcaf11	T	C	14: 55,803,152 (GRCm39)	I282T	probably damaging	Het
Dmxl1	T	C	18: 50,090,462 (GRCm39)		probably null	Het
Dnah11	T	G	12: 117,987,670 (GRCm39)	K2411Q	probably damaging	Het
Elf3	A	G	1: 135,184,220 (GRCm39)	V196A	probably benign	Het
Fhad1	T	G	4: 141,681,427 (GRCm39)	T538P	probably damaging	Het
Fkbp10	A	G	11: 100,311,849 (GRCm39)	D174G	probably damaging	Het
Gas6	A	G	8: 13,527,098 (GRCm39)	S217P	probably damaging	Het
Heatr5b	C	T	17: 79,074,304 (GRCm39)		probably null	Het
Iqgap3	A	G	3: 88,023,215 (GRCm39)	E502G	probably benign	Het
Klc1	T	C	12: 111,762,061 (GRCm39)	V577A	possibly damaging	Het
Lck	T	C	4: 129,445,431 (GRCm39)		probably null	Het
Mc3r	C	A	2: 172,091,610 (GRCm39)	Y277*	probably null	Het
Mlxipl	T	C	5: 135,162,458 (GRCm39)	V640A	probably benign	Het
Mrc2	A	T	11: 105,223,169 (GRCm39)	N471Y	probably damaging	Het
Ncan	T	A	8: 70,554,667 (GRCm39)	H1050L	probably damaging	Het
Nrl	C	A	14: 55,759,889 (GRCm39)	V13F	probably damaging	Het
Obscn	T	A	11: 58,967,082 (GRCm39)	Y546F	probably damaging	Het
Or52u1	T	G	7: 104,237,097 (GRCm39)	S46A	probably benign	Het
Or6c209	A	G	10: 129,483,960 (GRCm39)		probably null	Het
Or6d14	A	G	6: 116,534,074 (GRCm39)	I229M	probably damaging	Het
Pde3b	G	A	7: 114,120,927 (GRCm39)	G684D	probably damaging	Het
Prkaa1	C	T	15: 5,203,823 (GRCm39)	T244I	probably benign	Het
Scfd2	A	T	5: 74,367,059 (GRCm39)		probably null	Het
Slc22a30	G	A	19: 8,321,757 (GRCm39)	Q436*	probably null	Het
Smg1	A	T	7: 117,744,813 (GRCm39)	V3113D	possibly damaging	Het
Sorbs1	T	C	19: 40,365,325 (GRCm39)	T153A	probably benign	Het
Spag16	A	G	1: 69,909,448 (GRCm39)	T182A	probably benign	Het
Spopfm1	T	A	3: 94,173,959 (GRCm39)	H322Q	possibly damaging	Het
Svs5	T	C	2: 164,079,589 (GRCm39)	K106R	possibly damaging	Het
Tenm1	A	G	X: 42,163,572 (GRCm39)	V107A	possibly damaging	Het
Topaz1	A	T	9: 122,628,550 (GRCm39)	I1546F	possibly damaging	Het
Tubgcp2	C	A	7: 139,612,138 (GRCm39)	E78*	probably null	Het
Vars1	A	G	17: 35,224,457 (GRCm39)		probably null	Het

Other mutations in Vmn2r109

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01313:Vmn2r109	APN	17	20,770,419 (GRCm39)	missense	probably damaging	1.00
IGL01383:Vmn2r109	APN	17	20,761,383 (GRCm39)	missense	possibly damaging	0.89
IGL01469:Vmn2r109	APN	17	20,761,671 (GRCm39)	missense	probably damaging	1.00
IGL01762:Vmn2r109	APN	17	20,774,654 (GRCm39)	missense	probably benign
IGL01864:Vmn2r109	APN	17	20,761,396 (GRCm39)	missense	probably benign	0.28
IGL02028:Vmn2r109	APN	17	20,761,342 (GRCm39)	missense	probably benign	0.28
IGL02074:Vmn2r109	APN	17	20,774,603 (GRCm39)	missense	probably benign	0.05
IGL02162:Vmn2r109	APN	17	20,774,422 (GRCm39)	missense	probably benign	0.01
IGL02474:Vmn2r109	APN	17	20,761,150 (GRCm39)	missense	probably benign
IGL02490:Vmn2r109	APN	17	20,761,246 (GRCm39)	missense	possibly damaging	0.78
IGL02604:Vmn2r109	APN	17	20,760,963 (GRCm39)	missense	probably damaging	1.00
IGL02669:Vmn2r109	APN	17	20,774,518 (GRCm39)	missense	possibly damaging	0.64
IGL02705:Vmn2r109	APN	17	20,774,062 (GRCm39)	missense	probably benign
IGL02745:Vmn2r109	APN	17	20,761,512 (GRCm39)	missense	probably damaging	0.99
PIT4142001:Vmn2r109	UTSW	17	20,774,839 (GRCm39)	critical splice acceptor site	probably null
R0389:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R0470:Vmn2r109	UTSW	17	20,773,148 (GRCm39)	missense	probably benign	0.06
R0570:Vmn2r109	UTSW	17	20,760,937 (GRCm39)	missense	probably damaging	0.99
R0855:Vmn2r109	UTSW	17	20,761,670 (GRCm39)	nonsense	probably null
R0882:Vmn2r109	UTSW	17	20,774,842 (GRCm39)	splice site	probably benign
R1241:Vmn2r109	UTSW	17	20,775,503 (GRCm39)	missense	possibly damaging	0.86
R1587:Vmn2r109	UTSW	17	20,761,002 (GRCm39)	missense	probably damaging	1.00
R1931:Vmn2r109	UTSW	17	20,774,072 (GRCm39)	nonsense	probably null
R1957:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R1962:Vmn2r109	UTSW	17	20,774,185 (GRCm39)	missense	probably damaging	0.99
R2020:Vmn2r109	UTSW	17	20,761,448 (GRCm39)	nonsense	probably null
R2073:Vmn2r109	UTSW	17	20,784,974 (GRCm39)	missense	probably benign	0.00
R2436:Vmn2r109	UTSW	17	20,774,798 (GRCm39)	missense	probably damaging	0.99
R3123:Vmn2r109	UTSW	17	20,761,248 (GRCm39)	missense	probably damaging	1.00
R3839:Vmn2r109	UTSW	17	20,774,704 (GRCm39)	missense	probably damaging	1.00
R4019:Vmn2r109	UTSW	17	20,774,074 (GRCm39)	missense	probably benign
R4428:Vmn2r109	UTSW	17	20,773,286 (GRCm39)	missense	probably benign
R4584:Vmn2r109	UTSW	17	20,774,820 (GRCm39)	nonsense	probably null
R4652:Vmn2r109	UTSW	17	20,761,656 (GRCm39)	missense	probably damaging	1.00
R4708:Vmn2r109	UTSW	17	20,761,605 (GRCm39)	missense	probably damaging	0.97
R4823:Vmn2r109	UTSW	17	20,774,153 (GRCm39)	missense	probably damaging	1.00
R4831:Vmn2r109	UTSW	17	20,761,494 (GRCm39)	missense	probably benign	0.01
R4907:Vmn2r109	UTSW	17	20,770,348 (GRCm39)	missense	probably damaging	1.00
R5011:Vmn2r109	UTSW	17	20,775,451 (GRCm39)	missense	probably damaging	1.00
R5296:Vmn2r109	UTSW	17	20,774,603 (GRCm39)	missense	possibly damaging	0.90
R5600:Vmn2r109	UTSW	17	20,761,189 (GRCm39)	missense	probably damaging	1.00
R5602:Vmn2r109	UTSW	17	20,760,933 (GRCm39)	missense	possibly damaging	0.94
R5652:Vmn2r109	UTSW	17	20,760,781 (GRCm39)	makesense	probably null
R5702:Vmn2r109	UTSW	17	20,774,407 (GRCm39)	missense	probably benign	0.42
R5714:Vmn2r109	UTSW	17	20,773,121 (GRCm39)	missense	probably damaging	1.00
R5832:Vmn2r109	UTSW	17	20,761,318 (GRCm39)	missense	probably benign	0.10
R6008:Vmn2r109	UTSW	17	20,760,981 (GRCm39)	missense	probably damaging	1.00
R6334:Vmn2r109	UTSW	17	20,761,440 (GRCm39)	missense	probably benign	0.18
R6377:Vmn2r109	UTSW	17	20,784,796 (GRCm39)	critical splice donor site	probably null
R6738:Vmn2r109	UTSW	17	20,774,785 (GRCm39)	missense	possibly damaging	0.52
R6857:Vmn2r109	UTSW	17	20,760,932 (GRCm39)	missense	probably benign	0.45
R6953:Vmn2r109	UTSW	17	20,760,973 (GRCm39)	missense	possibly damaging	0.95
R7108:Vmn2r109	UTSW	17	20,785,006 (GRCm39)	missense	probably benign	0.03
R7229:Vmn2r109	UTSW	17	20,761,225 (GRCm39)	missense	possibly damaging	0.80
R7238:Vmn2r109	UTSW	17	20,761,336 (GRCm39)	missense	probably damaging	1.00
R7244:Vmn2r109	UTSW	17	20,760,945 (GRCm39)	missense	possibly damaging	0.70
R7292:Vmn2r109	UTSW	17	20,761,700 (GRCm39)	missense	probably benign	0.05
R7354:Vmn2r109	UTSW	17	20,761,043 (GRCm39)	missense	probably damaging	1.00
R7357:Vmn2r109	UTSW	17	20,761,536 (GRCm39)	missense	probably damaging	1.00
R7522:Vmn2r109	UTSW	17	20,774,665 (GRCm39)	missense	probably benign	0.11
R7596:Vmn2r109	UTSW	17	20,760,942 (GRCm39)	missense	probably damaging	0.98
R7728:Vmn2r109	UTSW	17	20,773,117 (GRCm39)	missense	probably damaging	0.99
R7859:Vmn2r109	UTSW	17	20,761,436 (GRCm39)	missense	probably damaging	1.00
R7871:Vmn2r109	UTSW	17	20,760,782 (GRCm39)	missense	probably benign	0.08
R8113:Vmn2r109	UTSW	17	20,774,729 (GRCm39)	missense	probably benign	0.01
R8153:Vmn2r109	UTSW	17	20,784,969 (GRCm39)	missense	probably benign	0.11
R8977:Vmn2r109	UTSW	17	20,774,531 (GRCm39)	missense	possibly damaging	0.96
R9687:Vmn2r109	UTSW	17	20,775,332 (GRCm39)	missense
Z1176:Vmn2r109	UTSW	17	20,773,256 (GRCm39)	missense	probably benign	0.00

Predicted Primers

PCR Primer
(F):5'- GCTACCATGGAATGAGTCAAACATG -3'
(R):5'- ATTCTTCGCTCTACCAGATGG -3'

Sequencing Primer
(F):5'- CAGACTTTCCCTGTCAACA -3'
(R):5'- CAAGTACACATTAATTCCACTTGGC -3'

Posted On

2017-01-03