Incidental Mutation 'R5730:Trak2'
ID 452696
Institutional Source Beutler Lab
Gene Symbol Trak2
Ensembl Gene ENSMUSG00000026028
Gene Name trafficking protein, kinesin binding 2
Synonyms 2900022D04Rik, GRIF-1, CALS-C, OIP98, GRIF1, Als2cr3, 4733401O11Rik
MMRRC Submission 043191-MU
Accession Numbers
Essential gene? Non essential (E-score: 0.000) question?
Stock # R5730 (G1)
Quality Score 225
Status Not validated
Chromosome 1
Chromosomal Location 58939608-59012589 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 58960966 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Aspartic acid to Glycine at position 188 (D188G)
Ref Sequence ENSEMBL: ENSMUSP00000027186 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000027186] [ENSMUST00000173590] [ENSMUST00000174120]
AlphaFold Q6P9N8
Predicted Effect probably damaging
Transcript: ENSMUST00000027186
AA Change: D188G

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)
SMART Domains Protein: ENSMUSP00000027186
Gene: ENSMUSG00000026028
AA Change: D188G

DomainStartEndE-ValueType
Pfam:HAP1_N 48 353 2.5e-135 PFAM
Pfam:Milton 426 565 3e-26 PFAM
low complexity region 663 673 N/A INTRINSIC
low complexity region 693 714 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000173590
SMART Domains Protein: ENSMUSP00000134499
Gene: ENSMUSG00000026028

DomainStartEndE-ValueType
Pfam:HAP1_N 2 52 9.4e-10 PFAM
Predicted Effect probably damaging
Transcript: ENSMUST00000174120
AA Change: D188G

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)
SMART Domains Protein: ENSMUSP00000134253
Gene: ENSMUSG00000026028
AA Change: D188G

DomainStartEndE-ValueType
Pfam:HAP1_N 47 354 1.3e-129 PFAM
Pfam:Milton 411 565 1.1e-41 PFAM
Predicted Effect noncoding transcript
Transcript: ENSMUST00000174359
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 97.1%
  • 20x: 95.1%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 42 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Ahnak T C 19: 8,987,617 (GRCm39) V2967A possibly damaging Het
Apaf1 A T 10: 90,856,633 (GRCm39) I858K possibly damaging Het
Baiap3 T C 17: 25,466,498 (GRCm39) T466A probably benign Het
Brca2 T A 5: 150,492,470 (GRCm39) S3162T possibly damaging Het
Brd1 T G 15: 88,601,248 (GRCm39) N462T probably benign Het
Carm1 G A 9: 21,491,636 (GRCm39) R235H probably benign Het
Cd80 T C 16: 38,303,097 (GRCm39) probably null Het
Clip4 A G 17: 72,117,954 (GRCm39) Y333C probably damaging Het
Col7a1 T C 9: 108,801,310 (GRCm39) probably null Het
Csmd1 G A 8: 16,235,206 (GRCm39) Q1206* probably null Het
Cyp17a1 A G 19: 46,661,095 (GRCm39) I63T possibly damaging Het
Diaph1 A G 18: 38,036,829 (GRCm39) Y119H unknown Het
Dst T G 1: 34,156,607 (GRCm39) probably null Het
Fgfr1 A G 8: 26,063,827 (GRCm39) T785A probably damaging Het
Gm1553 A G 10: 82,323,945 (GRCm39) F94S unknown Het
Gpbar1 A G 1: 74,318,195 (GRCm39) N146S probably damaging Het
Gpc5 A G 14: 116,025,726 (GRCm39) T588A possibly damaging Het
Gramd2a A G 9: 59,618,489 (GRCm39) H9R probably damaging Het
Klk1b11 G A 7: 43,424,199 (GRCm39) S6N probably benign Het
Lrp1 A G 10: 127,419,703 (GRCm39) S969P probably benign Het
Lyz2 G T 10: 117,114,587 (GRCm39) A114E probably damaging Het
Madd T C 2: 90,988,454 (GRCm39) D1193G probably damaging Het
Mrpl39 C T 16: 84,529,322 (GRCm39) G107R probably damaging Het
Mthfd2 C T 6: 83,294,441 (GRCm39) R24H probably benign Het
Or2q1 C T 6: 42,795,094 (GRCm39) R230* probably null Het
Or4a68 T C 2: 89,269,780 (GRCm39) N281S probably damaging Het
Ovch2 A G 7: 107,392,606 (GRCm39) C246R probably damaging Het
Phf14 A T 6: 11,953,319 (GRCm39) I353F possibly damaging Het
Ppp2r5a C T 1: 191,104,732 (GRCm39) V105I probably benign Het
Prr14l T C 5: 32,950,947 (GRCm39) T1949A probably damaging Het
Prss48 T G 3: 85,904,563 (GRCm39) M212L possibly damaging Het
Pstk A T 7: 130,975,503 (GRCm39) D152V probably damaging Het
Scn2a C A 2: 65,512,882 (GRCm39) S214* probably null Het
Scn3a T G 2: 65,325,604 (GRCm39) N971T probably benign Het
Syne3 T C 12: 104,927,713 (GRCm39) I250V probably benign Het
Synpo2 T C 3: 122,907,768 (GRCm39) D516G probably benign Het
Tcea3 T C 4: 135,992,204 (GRCm39) V209A probably benign Het
Tnr A G 1: 159,715,892 (GRCm39) S885G probably benign Het
Tubb1 A G 2: 174,299,562 (GRCm39) I415V probably benign Het
Virma T A 4: 11,542,154 (GRCm39) M1580K probably benign Het
Vmn1r120 A T 7: 20,786,934 (GRCm39) I259N possibly damaging Het
Vmn1r64 G A 7: 5,887,522 (GRCm39) T7I probably benign Het
Other mutations in Trak2
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01410:Trak2 APN 1 58,962,766 (GRCm39) missense probably damaging 1.00
IGL01982:Trak2 APN 1 58,965,814 (GRCm39) missense possibly damaging 0.94
IGL02154:Trak2 APN 1 58,947,888 (GRCm39) missense probably damaging 1.00
IGL02399:Trak2 APN 1 58,949,204 (GRCm39) missense probably benign 0.00
IGL02732:Trak2 APN 1 58,949,222 (GRCm39) missense probably benign 0.19
IGL02734:Trak2 APN 1 58,949,222 (GRCm39) missense probably benign 0.19
IGL03147:Trak2 UTSW 1 58,949,222 (GRCm39) missense probably benign 0.19
P0041:Trak2 UTSW 1 58,949,123 (GRCm39) missense probably damaging 1.00
R0079:Trak2 UTSW 1 58,965,883 (GRCm39) missense probably damaging 1.00
R0791:Trak2 UTSW 1 58,942,820 (GRCm39) missense probably benign
R0792:Trak2 UTSW 1 58,942,820 (GRCm39) missense probably benign
R1099:Trak2 UTSW 1 58,961,000 (GRCm39) missense probably benign 0.05
R1899:Trak2 UTSW 1 58,985,495 (GRCm39) start codon destroyed probably null 0.98
R1903:Trak2 UTSW 1 58,958,014 (GRCm39) splice site probably null
R2292:Trak2 UTSW 1 58,974,916 (GRCm39) missense probably damaging 0.99
R2312:Trak2 UTSW 1 58,974,941 (GRCm39) missense probably damaging 1.00
R3720:Trak2 UTSW 1 58,985,404 (GRCm39) critical splice donor site probably null
R4966:Trak2 UTSW 1 58,958,480 (GRCm39) missense probably damaging 1.00
R5088:Trak2 UTSW 1 58,974,967 (GRCm39) missense probably benign 0.06
R5840:Trak2 UTSW 1 58,958,432 (GRCm39) missense probably damaging 1.00
R5981:Trak2 UTSW 1 58,947,849 (GRCm39) missense probably benign 0.01
R6000:Trak2 UTSW 1 58,950,971 (GRCm39) missense possibly damaging 0.71
R6053:Trak2 UTSW 1 58,943,228 (GRCm39) missense possibly damaging 0.62
R6894:Trak2 UTSW 1 58,950,892 (GRCm39) missense probably damaging 1.00
R6916:Trak2 UTSW 1 58,949,184 (GRCm39) missense probably benign 0.06
R7096:Trak2 UTSW 1 58,942,749 (GRCm39) missense probably damaging 1.00
R7544:Trak2 UTSW 1 58,960,227 (GRCm39) splice site probably null
R7847:Trak2 UTSW 1 58,974,977 (GRCm39) missense possibly damaging 0.88
R7889:Trak2 UTSW 1 58,957,983 (GRCm39) missense probably damaging 1.00
R8039:Trak2 UTSW 1 58,985,447 (GRCm39) missense probably benign 0.16
R8313:Trak2 UTSW 1 58,960,306 (GRCm39) nonsense probably null
R8728:Trak2 UTSW 1 58,974,934 (GRCm39) missense probably benign 0.01
R8930:Trak2 UTSW 1 58,974,967 (GRCm39) missense probably benign
R8932:Trak2 UTSW 1 58,974,967 (GRCm39) missense probably benign
R9263:Trak2 UTSW 1 58,985,481 (GRCm39) missense probably benign 0.01
R9291:Trak2 UTSW 1 58,943,058 (GRCm39) missense probably damaging 1.00
R9404:Trak2 UTSW 1 58,960,296 (GRCm39) missense possibly damaging 0.53
R9670:Trak2 UTSW 1 58,985,463 (GRCm39) nonsense probably null
X0067:Trak2 UTSW 1 58,947,691 (GRCm39) missense probably benign 0.18
Predicted Primers PCR Primer
(F):5'- TCTCCTACGCACTCAGCAAG -3'
(R):5'- TAGCATCTGATGGAAAACCAACTG -3'

Sequencing Primer
(F):5'- GATGCTATGCTGGGTCTCACC -3'
(R):5'- CCAACTGTTCCGTGAATTAGGAGC -3'
Posted On 2017-01-03