Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL03054:Itgb4'

453032

Institutional Source

Beutler Lab

Gene Symbol

Itgb4

Ensembl Gene

ENSMUSG00000020758

Gene Name

integrin beta 4

Synonyms

CD104

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

IGL03054 (G1)

Quality Score

Status

Validated

Chromosome

Chromosomal Location

115865556-115899238 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

C to A at 115891166 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tyrosine to Stop codon at position 1190 (Y1190*)

Ref Sequence

ENSEMBL: ENSMUSP00000127604 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000021107] [ENSMUST00000068981] [ENSMUST00000106458] [ENSMUST00000106460] [ENSMUST00000106461] [ENSMUST00000169928]

AlphaFold

A2A863

Predicted Effect

probably null
Transcript: ENSMUST00000021107
AA Change: Y1190*

SMART Domains

Protein: ENSMUSP00000021107
Gene: ENSMUSG00000020758
AA Change: Y1190*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000068981
AA Change: Y1194*

SMART Domains

Protein: ENSMUSP00000070811
Gene: ENSMUSG00000020758
AA Change: Y1194*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1459	1540	1.79e-12	SMART
FN3	1572	1656	1.7e-13	SMART
low complexity region	1689	1702	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106458
AA Change: Y1194*

SMART Domains

Protein: ENSMUSP00000102066
Gene: ENSMUSG00000020758
AA Change: Y1194*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106460
AA Change: Y1194*

SMART Domains

Protein: ENSMUSP00000102068
Gene: ENSMUSG00000020758
AA Change: Y1194*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
FN3	1512	1593	1.79e-12	SMART
FN3	1625	1709	1.7e-13	SMART
low complexity region	1742	1755	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000106461
AA Change: Y1194*

SMART Domains

Protein: ENSMUSP00000102069
Gene: ENSMUSG00000020758
AA Change: Y1194*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	713	7.06e-29	SMART
low complexity region	716	733	N/A	INTRINSIC
transmembrane domain	738	757	N/A	INTRINSIC
Calx_beta	981	1086	3.13e-35	SMART
FN3	1129	1207	3.15e-8	SMART
FN3	1222	1309	6.29e-8	SMART
low complexity region	1328	1336	N/A	INTRINSIC
low complexity region	1413	1425	N/A	INTRINSIC
FN3	1524	1605	1.79e-12	SMART
FN3	1637	1721	1.7e-13	SMART
low complexity region	1754	1767	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000169928
AA Change: Y1190*

SMART Domains

Protein: ENSMUSP00000127604
Gene: ENSMUSG00000020758
AA Change: Y1190*

Domain	Start	End	E-Value	Type
signal peptide	1	28	N/A	INTRINSIC
PSI	30	74	3.89e-1	SMART
INB	38	457	4.23e-213	SMART
VWA	130	361	8.29e-1	SMART
EGF_like	544	576	3.23e1	SMART
Integrin_B_tail	628	712	1.32e-28	SMART
low complexity region	715	732	N/A	INTRINSIC
transmembrane domain	737	756	N/A	INTRINSIC
Calx_beta	980	1085	3.13e-35	SMART
FN3	1125	1203	3.15e-8	SMART
FN3	1218	1305	6.29e-8	SMART
low complexity region	1324	1332	N/A	INTRINSIC
FN3	1508	1589	1.79e-12	SMART
FN3	1621	1705	1.7e-13	SMART
low complexity region	1738	1751	N/A	INTRINSIC

Meta Mutation Damage Score

0.9755

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

98% (43/44)

MGI Phenotype

FUNCTION: Integrins are heterodimers comprised of alpha and beta subunits, that are noncovalently associated transmembrane glycoprotein receptors. Different combinations of alpha and beta polypeptides form complexes that vary in their ligand-binding specificities. Integrins mediate cell-matrix or cell-cell adhesion, and transduced signals that regulate gene expression and cell growth. This gene encodes the integrin beta 4 subunit, a receptor for the laminins. This subunit tends to associate with alpha 6 subunit and is likely to play a pivotal role in the biology of invasive carcinoma. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygotes for targeted null mutations die shortly after birth with extensive detachment of the epidermis and other squamus epithelia. Stratified tissues lack hemidesmosomes and simple epithelia are also defective in adherence. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 35 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4933416I08Rik	TCC	TCCC	X: 52,692,862 (GRCm39)		noncoding transcript	Het
Acsbg3	A	G	17: 57,193,528 (GRCm39)	T625A	possibly damaging	Het
Aloxe3	T	C	11: 69,020,433 (GRCm39)	V159A	possibly damaging	Het
Atp13a2	T	A	4: 140,734,279 (GRCm39)	C1134S	possibly damaging	Het
Ccdc40	G	A	11: 119,154,027 (GRCm39)	E1100K	possibly damaging	Het
Ceacam5	A	T	7: 17,493,379 (GRCm39)	T801S	possibly damaging	Het
Cilp	TGGG	TGG	9: 65,187,412 (GRCm39)		probably null	Het
Col4a2	T	C	8: 11,498,270 (GRCm39)	I1693T	probably damaging	Het
Crb1	CG	C	1: 139,164,824 (GRCm39)		probably null	Het
Dab2	G	T	15: 6,447,707 (GRCm39)		probably benign	Het
Dao	T	C	5: 114,162,963 (GRCm39)	L345P	probably damaging	Het
Dis3l	A	G	9: 64,217,722 (GRCm39)		probably null	Het
Egr3	C	T	14: 70,316,561 (GRCm39)	T124M	probably damaging	Het
Gng7	G	T	10: 80,787,485 (GRCm39)	F59L	probably damaging	Het
Lacc1	T	C	14: 77,268,355 (GRCm39)	M319V	possibly damaging	Het
Mapkbp1	A	G	2: 119,845,881 (GRCm39)	T417A	probably damaging	Het
Mier3	C	T	13: 111,822,848 (GRCm39)		probably benign	Het
Mlxipl	A	G	5: 135,162,110 (GRCm39)	D569G	possibly damaging	Het
Mmp1a	TG	TGG	9: 7,465,083 (GRCm38)		probably null	Het
Mrpl15	A	G	1: 4,855,794 (GRCm39)		probably null	Het
Neb	T	C	2: 52,161,334 (GRCm39)	N2153D	probably damaging	Het
Nlrp9c	A	G	7: 26,081,701 (GRCm39)		probably null	Het
Npepps	C	T	11: 97,132,614 (GRCm39)		probably benign	Het
Or13a17	T	G	7: 140,271,623 (GRCm39)	S268R	probably benign	Het
Or1j10	A	T	2: 36,266,944 (GRCm39)	H52L	possibly damaging	Het
Or52z12	C	A	7: 103,234,047 (GRCm39)	H273N	probably benign	Het
Psmc4	T	C	7: 27,746,605 (GRCm39)	Y160C	probably damaging	Het
Rims1	T	C	1: 22,360,333 (GRCm39)	Y131C	probably damaging	Het
Riok1	G	A	13: 38,231,291 (GRCm39)	G183D	probably damaging	Het
Samd9l	T	A	6: 3,376,023 (GRCm39)	I413F	probably damaging	Het
Tnfrsf22	A	G	7: 143,194,532 (GRCm39)	Y132H	probably damaging	Het
Ttn	T	A	2: 76,726,104 (GRCm39)		probably benign	Het
Tulp1	A	G	17: 28,578,287 (GRCm39)		probably benign	Het
Usp15	C	A	10: 122,961,836 (GRCm39)		probably benign	Het
Wdr7	G	T	18: 63,958,192 (GRCm39)		probably benign	Het

Other mutations in Itgb4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01360:Itgb4	APN	11	115,881,766 (GRCm39)	missense	probably damaging	1.00
IGL01391:Itgb4	APN	11	115,881,746 (GRCm39)	missense	probably damaging	1.00
IGL01431:Itgb4	APN	11	115,897,283 (GRCm39)	splice site	probably benign
IGL01750:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01752:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01756:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01766:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL01769:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02188:Itgb4	APN	11	115,894,213 (GRCm39)	missense	probably benign	0.08
IGL02262:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02293:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02318:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02319:Itgb4	APN	11	115,879,752 (GRCm39)	missense	probably damaging	0.99
IGL02338:Itgb4	APN	11	115,898,795 (GRCm39)	missense	probably damaging	1.00
IGL02734:Itgb4	APN	11	115,896,792 (GRCm39)	missense	probably benign
IGL02879:Itgb4	APN	11	115,885,178 (GRCm39)	missense	probably benign	0.05
IGL02889:Itgb4	APN	11	115,879,731 (GRCm39)	missense	probably damaging	1.00
IGL03183:Itgb4	APN	11	115,879,550 (GRCm39)	missense	probably damaging	1.00
R0021:Itgb4	UTSW	11	115,870,453 (GRCm39)	missense	possibly damaging	0.95
R0092:Itgb4	UTSW	11	115,869,950 (GRCm39)	missense	probably damaging	1.00
R0305:Itgb4	UTSW	11	115,870,238 (GRCm39)	missense	probably damaging	1.00
R0408:Itgb4	UTSW	11	115,898,428 (GRCm39)	missense	probably damaging	0.99
R0465:Itgb4	UTSW	11	115,870,582 (GRCm39)	missense	probably damaging	1.00
R0499:Itgb4	UTSW	11	115,870,521 (GRCm39)	missense	probably benign	0.00
R0535:Itgb4	UTSW	11	115,881,835 (GRCm39)	missense	possibly damaging	0.86
R0571:Itgb4	UTSW	11	115,870,594 (GRCm39)	missense	possibly damaging	0.94
R0613:Itgb4	UTSW	11	115,884,168 (GRCm39)	missense	probably damaging	0.98
R0838:Itgb4	UTSW	11	115,888,988 (GRCm39)	intron	probably benign
R1381:Itgb4	UTSW	11	115,885,163 (GRCm39)	missense	probably benign	0.00
R1451:Itgb4	UTSW	11	115,881,710 (GRCm39)	missense	probably damaging	1.00
R1459:Itgb4	UTSW	11	115,869,937 (GRCm39)	missense	probably benign	0.42
R1460:Itgb4	UTSW	11	115,874,990 (GRCm39)	missense	probably damaging	0.96
R1473:Itgb4	UTSW	11	115,874,873 (GRCm39)	missense	probably benign	0.01
R1484:Itgb4	UTSW	11	115,890,625 (GRCm39)	missense	probably benign	0.01
R1593:Itgb4	UTSW	11	115,871,817 (GRCm39)	missense	probably damaging	1.00
R1623:Itgb4	UTSW	11	115,882,142 (GRCm39)	nonsense	probably null
R1633:Itgb4	UTSW	11	115,898,586 (GRCm39)	missense	probably damaging	1.00
R1642:Itgb4	UTSW	11	115,898,183 (GRCm39)	missense	probably damaging	1.00
R1669:Itgb4	UTSW	11	115,882,156 (GRCm39)	missense	probably benign	0.07
R1713:Itgb4	UTSW	11	115,894,315 (GRCm39)	missense	probably damaging	1.00
R1732:Itgb4	UTSW	11	115,879,744 (GRCm39)	missense	probably damaging	1.00
R1791:Itgb4	UTSW	11	115,879,346 (GRCm39)	missense	probably damaging	1.00
R1847:Itgb4	UTSW	11	115,874,590 (GRCm39)	missense	probably benign	0.31
R1902:Itgb4	UTSW	11	115,871,564 (GRCm39)	missense	probably damaging	0.98
R1945:Itgb4	UTSW	11	115,884,279 (GRCm39)	nonsense	probably null
R2102:Itgb4	UTSW	11	115,896,561 (GRCm39)	missense	probably benign	0.23
R2184:Itgb4	UTSW	11	115,870,450 (GRCm39)	missense	probably damaging	0.96
R2334:Itgb4	UTSW	11	115,884,261 (GRCm39)	missense	probably damaging	1.00
R2401:Itgb4	UTSW	11	115,897,389 (GRCm39)	missense	possibly damaging	0.67
R3743:Itgb4	UTSW	11	115,894,496 (GRCm39)	missense	probably damaging	1.00
R3938:Itgb4	UTSW	11	115,896,752 (GRCm39)	missense	possibly damaging	0.92
R4134:Itgb4	UTSW	11	115,897,296 (GRCm39)	missense	probably benign	0.03
R4280:Itgb4	UTSW	11	115,881,761 (GRCm39)	missense	probably damaging	1.00
R4342:Itgb4	UTSW	11	115,879,555 (GRCm39)	missense	probably benign	0.01
R4434:Itgb4	UTSW	11	115,890,640 (GRCm39)	missense	probably benign	0.10
R4505:Itgb4	UTSW	11	115,874,087 (GRCm39)	splice site	silent
R4585:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4586:Itgb4	UTSW	11	115,884,151 (GRCm39)	missense	probably damaging	1.00
R4601:Itgb4	UTSW	11	115,896,548 (GRCm39)	missense	probably damaging	1.00
R4921:Itgb4	UTSW	11	115,897,431 (GRCm39)	missense	probably benign	0.12
R4962:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5027:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5029:Itgb4	UTSW	11	115,879,417 (GRCm39)	intron	probably benign
R5084:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5085:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5124:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5125:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5150:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5175:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5176:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5179:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5207:Itgb4	UTSW	11	115,897,365 (GRCm39)	missense	probably damaging	1.00
R5263:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5264:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5334:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5337:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5344:Itgb4	UTSW	11	115,880,575 (GRCm39)	missense	probably null	0.92
R5391:Itgb4	UTSW	11	115,875,894 (GRCm39)	missense	probably benign	0.05
R5437:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5440:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5653:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5654:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5655:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5772:Itgb4	UTSW	11	115,879,258 (GRCm39)	intron	probably benign
R5812:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5813:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5814:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5863:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5864:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5865:Itgb4	UTSW	11	115,881,748 (GRCm39)	missense	probably damaging	1.00
R5951:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5954:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R5982:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6043:Itgb4	UTSW	11	115,870,212 (GRCm39)	missense	probably benign	0.30
R6133:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6134:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6135:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6169:Itgb4	UTSW	11	115,885,102 (GRCm39)	missense	probably damaging	0.98
R6172:Itgb4	UTSW	11	115,891,237 (GRCm39)	missense	probably benign	0.23
R6255:Itgb4	UTSW	11	115,888,963 (GRCm39)	missense	possibly damaging	0.83
R6258:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6259:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6260:Itgb4	UTSW	11	115,874,983 (GRCm39)	missense	probably benign	0.00
R6612:Itgb4	UTSW	11	115,874,897 (GRCm39)	missense	probably benign	0.00
R7037:Itgb4	UTSW	11	115,896,391 (GRCm39)	nonsense	probably null
R7371:Itgb4	UTSW	11	115,888,906 (GRCm39)	missense	probably benign	0.29
R7605:Itgb4	UTSW	11	115,897,302 (GRCm39)	missense	probably benign	0.01
R7659:Itgb4	UTSW	11	115,870,557 (GRCm39)	missense	probably damaging	1.00
R7759:Itgb4	UTSW	11	115,894,536 (GRCm39)	missense	possibly damaging	0.92
R7804:Itgb4	UTSW	11	115,894,510 (GRCm39)	missense	probably damaging	1.00
R7832:Itgb4	UTSW	11	115,891,087 (GRCm39)	missense	probably damaging	1.00
R7842:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.18
R7923:Itgb4	UTSW	11	115,873,525 (GRCm39)	critical splice acceptor site	probably null
R8004:Itgb4	UTSW	11	115,873,531 (GRCm39)	missense	probably benign	0.00
R8143:Itgb4	UTSW	11	115,884,255 (GRCm39)	missense	probably damaging	1.00
R8427:Itgb4	UTSW	11	115,882,544 (GRCm39)	critical splice donor site	probably null
R8857:Itgb4	UTSW	11	115,871,853 (GRCm39)	missense	probably benign	0.04
R8863:Itgb4	UTSW	11	115,875,898 (GRCm39)	nonsense	probably null
R8932:Itgb4	UTSW	11	115,879,295 (GRCm39)	missense	probably benign	0.01
R9153:Itgb4	UTSW	11	115,874,879 (GRCm39)	missense	probably benign	0.00
R9207:Itgb4	UTSW	11	115,897,923 (GRCm39)	missense	probably damaging	1.00
R9239:Itgb4	UTSW	11	115,898,130 (GRCm39)	missense	probably damaging	1.00
R9267:Itgb4	UTSW	11	115,870,465 (GRCm39)	missense	probably benign
R9289:Itgb4	UTSW	11	115,885,187 (GRCm39)	missense	probably benign	0.01
R9328:Itgb4	UTSW	11	115,880,625 (GRCm39)	missense	probably benign	0.00
R9435:Itgb4	UTSW	11	115,895,855 (GRCm39)	missense	probably benign	0.01
R9450:Itgb4	UTSW	11	115,874,097 (GRCm39)	missense	probably damaging	1.00
R9649:Itgb4	UTSW	11	115,885,171 (GRCm39)	missense	possibly damaging	0.78
R9779:Itgb4	UTSW	11	115,882,485 (GRCm39)	missense	probably damaging	1.00
X0062:Itgb4	UTSW	11	115,884,278 (GRCm39)	missense	probably damaging	1.00
Z1176:Itgb4	UTSW	11	115,897,346 (GRCm39)	missense	probably damaging	1.00
Z1177:Itgb4	UTSW	11	115,888,884 (GRCm39)	missense	probably benign	0.00
Z1177:Itgb4	UTSW	11	115,877,637 (GRCm39)	missense	probably damaging	0.99

Predicted Primers

PCR Primer
(F):5'- AGCCACCAATGTCACTTGTAC -3'
(R):5'- ATTGAAAGCCAGCCTCCCTG -3'

Sequencing Primer
(F):5'- TCACTTGTACAAAGGGAGTCCTAGC -3'
(R):5'- GCAGAAAGGGCCCTATTACAG -3'

Posted On

2017-01-24