Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL02984:Foxi2'

453272

Institutional Source

Beutler Lab

Gene Symbol

Foxi2

Ensembl Gene

ENSMUSG00000048377

Gene Name

forkhead box I2

Synonyms

B130055A05Rik

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

IGL02984 (G1)

Quality Score

109

Status

Validated

Chromosome

Chromosomal Location

135012096-135015351 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 135012127 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Methionine at position 5 (T5M)

Ref Sequence

ENSEMBL: ENSMUSP00000053641 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000060356]

AlphaFold

Q3I5G5

Predicted Effect

possibly damaging
Transcript: ENSMUST00000060356
AA Change: T5M

PolyPhen 2 Score 0.956 (Sensitivity: 0.79; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000053641
Gene: ENSMUSG00000048377
AA Change: T5M

Domain	Start	End	E-Value	Type
low complexity region	64	82	N/A	INTRINSIC
low complexity region	87	108	N/A	INTRINSIC
FH	115	205	1.29e-58	SMART
low complexity region	207	213	N/A	INTRINSIC
low complexity region	237	249	N/A	INTRINSIC
low complexity region	262	278	N/A	INTRINSIC

Meta Mutation Damage Score

0.1795

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

100% (43/43)

MGI Phenotype

PHENOTYPE: Mice homozygous for a null allele are viable and fertile with no gross abnormalities. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 36 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2310003L06Rik	T	A	5: 88,120,662 (GRCm39)	I473N	probably damaging	Het
4933416I08Rik	TCC	TCCC	X: 52,692,862 (GRCm39)		noncoding transcript	Het
A530064D06Rik	T	C	17: 48,470,448 (GRCm39)	I178V	probably benign	Het
Acvr1b	A	G	15: 101,100,959 (GRCm39)	R374G	probably damaging	Het
Aldh1l2	G	A	10: 83,363,199 (GRCm39)	P55S	probably damaging	Het
Bglap3	T	C	3: 88,276,098 (GRCm39)	T85A	possibly damaging	Het
Cilp	TGGG	TGG	9: 65,187,412 (GRCm39)		probably null	Het
Crb1	CG	C	1: 139,164,824 (GRCm39)		probably null	Het
Csrnp3	A	G	2: 65,852,553 (GRCm39)	D315G	probably benign	Het
Dclk3	T	C	9: 111,317,643 (GRCm39)	Y760H	probably damaging	Het
Eef1akmt2	A	G	7: 132,438,935 (GRCm39)	*52R	probably null	Het
Epc2	A	G	2: 49,418,866 (GRCm39)	K225E	probably damaging	Het
Fcna	G	C	2: 25,520,693 (GRCm39)		probably benign	Het
Frmd4b	T	A	6: 97,273,221 (GRCm39)	T670S	probably damaging	Het
Gm14137	G	T	2: 119,005,961 (GRCm39)	E173D	probably damaging	Het
Kif12	GGGGC	GGGGCCTCCACCCGGCGGGC	4: 63,089,660 (GRCm39)		probably benign	Het
Mfsd4b3-ps	G	A	10: 39,823,184 (GRCm39)		probably benign	Het
Mlst8	A	G	17: 24,695,127 (GRCm39)	F252S	probably damaging	Het
Mmp1a	TG	TGG	9: 7,465,083 (GRCm38)		probably null	Het
Mogs	A	G	6: 83,094,296 (GRCm39)	K371R	probably benign	Het
Nsun2	T	C	13: 69,691,727 (GRCm39)		probably benign	Het
Otog	T	A	7: 45,954,932 (GRCm39)	C2702S	probably damaging	Het
Plekhg4	A	G	8: 106,107,020 (GRCm39)	E905G	probably damaging	Het
Rtn4rl1	T	C	11: 75,156,087 (GRCm39)	V173A	probably benign	Het
Sall3	T	C	18: 81,016,665 (GRCm39)	E421G	probably benign	Het
Setd6	G	A	8: 96,442,903 (GRCm39)		probably null	Het
Sh3tc1	T	C	5: 35,871,403 (GRCm39)		probably null	Het
Slc35f5	T	A	1: 125,490,250 (GRCm39)	Y71N	probably benign	Het
Snx1	A	G	9: 65,996,390 (GRCm39)		probably benign	Het
Speer4c1	A	C	5: 15,919,214 (GRCm39)		probably benign	Het
Sspo	T	C	6: 48,472,089 (GRCm39)	V792A	probably benign	Het
Sufu	C	A	19: 46,462,038 (GRCm39)	D350E	probably benign	Het
Trav18	C	A	14: 54,069,026 (GRCm39)	Q23K	probably damaging	Het
Ugt1a1	AT	A	1: 88,140,093 (GRCm39)		probably null	Het
Usp17le	T	A	7: 104,418,311 (GRCm39)	H277L	probably benign	Het
Wdsub1	A	G	2: 59,707,173 (GRCm39)	S20P	probably damaging	Het

Other mutations in Foxi2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01101:Foxi2	APN	7	135,013,736 (GRCm39)	missense	probably benign	0.07
R0030:Foxi2	UTSW	7	135,013,345 (GRCm39)	missense	probably damaging	0.99
R0030:Foxi2	UTSW	7	135,013,345 (GRCm39)	missense	probably damaging	0.99
R0121:Foxi2	UTSW	7	135,013,640 (GRCm39)	missense	probably benign
R0830:Foxi2	UTSW	7	135,013,459 (GRCm39)	missense	probably benign
R2059:Foxi2	UTSW	7	135,012,406 (GRCm39)	missense	probably damaging	1.00
R3615:Foxi2	UTSW	7	135,012,180 (GRCm39)	missense	possibly damaging	0.88
R3616:Foxi2	UTSW	7	135,012,180 (GRCm39)	missense	possibly damaging	0.88
R4021:Foxi2	UTSW	7	135,012,259 (GRCm39)	missense	probably damaging	1.00
R4764:Foxi2	UTSW	7	135,012,396 (GRCm39)	missense	probably damaging	1.00
R5048:Foxi2	UTSW	7	135,013,364 (GRCm39)	missense	probably damaging	1.00
R5257:Foxi2	UTSW	7	135,012,256 (GRCm39)	missense	probably benign	0.19
R5258:Foxi2	UTSW	7	135,012,256 (GRCm39)	missense	probably benign	0.19
R5561:Foxi2	UTSW	7	135,013,376 (GRCm39)	missense	probably damaging	1.00
R5611:Foxi2	UTSW	7	135,013,433 (GRCm39)	missense	probably benign	0.00
R5667:Foxi2	UTSW	7	135,012,668 (GRCm39)	splice site	probably null
R7492:Foxi2	UTSW	7	135,012,301 (GRCm39)	missense	probably damaging	0.99
R8726:Foxi2	UTSW	7	135,012,133 (GRCm39)	missense	probably damaging	0.98
R9205:Foxi2	UTSW	7	135,013,525 (GRCm39)	missense	probably benign
R9644:Foxi2	UTSW	7	135,013,727 (GRCm39)	missense	possibly damaging	0.95
Z1177:Foxi2	UTSW	7	135,013,687 (GRCm39)	missense	probably benign	0.32
Z1177:Foxi2	UTSW	7	135,012,144 (GRCm39)	missense	probably benign	0.40

Predicted Primers

PCR Primer
(F):5'- TCGGAGAGTTCCAAGACTGTTTG -3'
(R):5'- ATGGCGATCAGTGCTGAGTAG -3'

Sequencing Primer
(F):5'- GACTGTTTGTCTGGCTGTCCC -3'
(R):5'- GCTCAGCCAAGCGAGATCAG -3'

Posted On

2017-02-01