Mutagenetix > Incidental Mutation

Incidental Mutation 'IGL03055:Nol4l'

453372

Institutional Source

Beutler Lab

Gene Symbol

Nol4l

Ensembl Gene

ENSMUSG00000061411

Gene Name

nucleolar protein 4-like

Synonyms

8430427H17Rik, LOC381396

Accession Numbers

Essential gene?

Probably essential (E-score: 0.901) question?

Stock #

IGL03055 (G1)

Quality Score

199

Status

Validated

Chromosome

Chromosomal Location

153249381-153371869 bp(-) (GRCm39)

Type of Mutation

synonymous

DNA Base Change (assembly)

G to C at 153278190 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000105407 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000035346] [ENSMUST00000109784]

AlphaFold

no structure available at present

Predicted Effect

silent
Transcript: ENSMUST00000035346

SMART Domains

Protein: ENSMUSP00000036571
Gene: ENSMUSG00000061411

Domain	Start	End	E-Value	Type
low complexity region	58	71	N/A	INTRINSIC
low complexity region	277	305	N/A	INTRINSIC
low complexity region	405	422	N/A	INTRINSIC
low complexity region	619	639	N/A	INTRINSIC

Predicted Effect

silent
Transcript: ENSMUST00000109784

SMART Domains

Protein: ENSMUSP00000105407
Gene: ENSMUSG00000061411

Domain	Start	End	E-Value	Type
low complexity region	33	61	N/A	INTRINSIC
SCOP:d1sig__	161	246	1e-2	SMART
low complexity region	375	395	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000132770

Coding Region Coverage

1x: 0.0%
3x: 0.0%
10x: 0.0%
20x: 0.0%

Validation Efficiency

98% (58/59)

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
2900041M22Rik	T	A	11: 117,503,072 (GRCm39)		noncoding transcript	Het
4933416I08Rik	TCC	TCCC	X: 52,692,862 (GRCm39)		noncoding transcript	Het
A430005L14Rik	T	A	4: 154,045,092 (GRCm39)	S40R	probably benign	Het
Aire	A	G	10: 77,878,903 (GRCm39)	L48P	probably damaging	Het
Alkbh8	T	C	9: 3,345,882 (GRCm39)		probably benign	Het
Aqp7	A	G	4: 41,045,326 (GRCm39)	M18T	probably benign	Het
Cchcr1	T	A	17: 35,837,516 (GRCm39)	M406K	probably benign	Het
Cdhr1	A	T	14: 36,817,054 (GRCm39)	D102E	probably benign	Het
Cilp	TGGG	TGG	9: 65,187,412 (GRCm39)		probably null	Het
Clec16a	T	A	16: 10,559,645 (GRCm39)	S973T	probably damaging	Het
Crb1	CG	C	1: 139,164,824 (GRCm39)		probably null	Het
Csmd1	A	G	8: 16,145,515 (GRCm39)	Y1471H	probably damaging	Het
Dnah12	A	G	14: 26,594,697 (GRCm39)	Y3489C	probably damaging	Het
Ect2	T	C	3: 27,191,211 (GRCm39)	E464G	probably damaging	Het
Enpp2	C	A	15: 54,729,481 (GRCm39)		probably null	Het
Erich2	G	T	2: 70,339,529 (GRCm39)	C28F	possibly damaging	Het
Fam135b	A	T	15: 71,493,883 (GRCm39)	H15Q	possibly damaging	Het
Fcna	G	C	2: 25,520,693 (GRCm39)		probably benign	Het
Gfral	T	A	9: 76,115,831 (GRCm39)	T48S	probably benign	Het
Itga10	A	G	3: 96,557,836 (GRCm39)	E293G	probably damaging	Het
Lrp2	A	T	2: 69,288,792 (GRCm39)	N3691K	probably damaging	Het
Mmp1a	TG	TGG	9: 7,465,083 (GRCm38)		probably null	Het
Nefm	T	C	14: 68,360,358 (GRCm39)	T371A	probably damaging	Het
Nkain3	C	A	4: 20,778,308 (GRCm39)	C3F	probably benign	Het
Nprl3	C	A	11: 32,198,230 (GRCm39)		probably benign	Het
Or52p1	A	T	7: 104,267,413 (GRCm39)	I176F	probably damaging	Het
Pate2	T	C	9: 35,523,069 (GRCm39)		probably benign	Het
Pde4d	A	G	13: 110,071,879 (GRCm39)	K128R	probably damaging	Het
Ptprt	A	G	2: 161,375,533 (GRCm39)	L1329P	probably damaging	Het
Rapgef3	T	C	15: 97,647,370 (GRCm39)		probably benign	Het
Rassf5	T	C	1: 131,172,732 (GRCm39)	I46V	probably benign	Het
Rin1	C	T	19: 5,103,187 (GRCm39)	T481I	probably benign	Het
Samd4b	A	G	7: 28,104,971 (GRCm39)	L524P	possibly damaging	Het
Speer4c1	A	C	5: 15,919,214 (GRCm39)		probably benign	Het
Srebf1	T	C	11: 60,097,902 (GRCm39)		silent	Het
Ssh2	C	T	11: 77,299,021 (GRCm39)	Q123*	probably null	Het
St18	G	T	1: 6,872,959 (GRCm39)	L231F	probably damaging	Het
Stk11	A	C	10: 79,963,920 (GRCm39)	D96A	probably damaging	Het
Svil	A	G	18: 5,108,615 (GRCm39)	T1918A	probably damaging	Het
Thrap3	G	A	4: 126,059,335 (GRCm39)		probably null	Het
Tlr1	C	A	5: 65,083,939 (GRCm39)	V213F	probably benign	Het
Trappc10	C	T	10: 78,050,520 (GRCm39)	R307Q	probably damaging	Het
Ugt1a1	AT	A	1: 88,140,093 (GRCm39)		probably null	Het
Wnt6	G	A	1: 74,822,013 (GRCm39)	R198H	probably damaging	Het
Yipf2	A	T	9: 21,501,019 (GRCm39)	V98E	probably benign	Het
Zc3h15	A	G	2: 83,491,515 (GRCm39)	T248A	possibly damaging	Het
Zc3hav1	C	T	6: 38,293,251 (GRCm39)		probably null	Het
Zfp697	T	G	3: 98,332,810 (GRCm39)	C79G	possibly damaging	Het

Other mutations in Nol4l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00933:Nol4l	APN	2	153,319,856 (GRCm39)	missense	probably damaging	0.96
IGL01325:Nol4l	APN	2	153,278,271 (GRCm39)	splice site	probably benign
IGL02608:Nol4l	APN	2	153,278,213 (GRCm39)	missense	possibly damaging	0.50
IGL02886:Nol4l	APN	2	153,371,457 (GRCm39)	missense	probably benign	0.27
IGL03210:Nol4l	APN	2	153,371,378 (GRCm39)	missense	probably benign	0.03
R0285:Nol4l	UTSW	2	153,325,773 (GRCm39)	splice site	probably benign
R0345:Nol4l	UTSW	2	153,253,672 (GRCm39)	missense	probably benign	0.00
R0555:Nol4l	UTSW	2	153,259,604 (GRCm39)	splice site	probably null
R1966:Nol4l	UTSW	2	153,371,375 (GRCm39)	missense	probably benign	0.01
R2044:Nol4l	UTSW	2	153,371,441 (GRCm39)	missense	possibly damaging	0.66
R2368:Nol4l	UTSW	2	153,259,959 (GRCm39)	missense	probably damaging	1.00
R4855:Nol4l	UTSW	2	153,253,726 (GRCm39)	missense	probably benign	0.06
R5696:Nol4l	UTSW	2	153,260,026 (GRCm39)	missense	probably damaging	0.99
R5776:Nol4l	UTSW	2	153,259,741 (GRCm39)	missense	probably damaging	1.00
R6807:Nol4l	UTSW	2	153,325,746 (GRCm39)	nonsense	probably null
R6845:Nol4l	UTSW	2	153,258,582 (GRCm39)	missense	probably benign	0.00
R6872:Nol4l	UTSW	2	153,325,737 (GRCm39)	missense	probably damaging	0.98
R6940:Nol4l	UTSW	2	153,253,684 (GRCm39)	missense	probably benign	0.00
R8165:Nol4l	UTSW	2	153,262,473 (GRCm39)	nonsense	probably null
R8263:Nol4l	UTSW	2	153,259,337 (GRCm39)	missense	probably damaging	0.99
R8500:Nol4l	UTSW	2	153,278,266 (GRCm39)	missense	probably damaging	0.99
R8938:Nol4l	UTSW	2	153,262,651 (GRCm39)	missense	probably damaging	1.00
R9097:Nol4l	UTSW	2	153,312,630 (GRCm39)	missense	probably damaging	0.96
R9098:Nol4l	UTSW	2	153,312,630 (GRCm39)	missense	probably damaging	0.96
R9099:Nol4l	UTSW	2	153,312,630 (GRCm39)	missense	probably damaging	0.96
R9115:Nol4l	UTSW	2	153,253,638 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACCAGCTTTCAAGTACAACAGG -3'
(R):5'- GGAGAGTACCATTCAGCACTC -3'

Sequencing Primer
(F):5'- GAACCACAAGTGTCACCTGGG -3'
(R):5'- ACGTTCTTGGGGCTCCCTG -3'

Posted On

2017-02-03