Incidental Mutation 'IGL03050:Ugt8a'
| ID |
453426 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Ugt8a
|
| Ensembl Gene |
ENSMUSG00000032854 |
| Gene Name |
UDP galactosyltransferase 8A |
| Synonyms |
Ugt8, Cgt, mCerGT |
| Accession Numbers |
|
| Essential gene? |
Possibly essential
(E-score: 0.719)
|
| Stock # |
IGL03050 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
3 |
| Chromosomal Location |
125658920-125732268 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
T to C
at 125669139 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Arginine to Glycine
at position 322
(R322G)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000143605
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000057944]
[ENSMUST00000198610]
|
| AlphaFold |
Q64676 |
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000057944
AA Change: R322G
PolyPhen 2
Score 0.625 (Sensitivity: 0.87; Specificity: 0.91)
|
| SMART Domains |
Protein: ENSMUSP00000050852
Gene: ENSMUSG00000032854
AA Change: R322G
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:UDPGT
|
21 |
510 |
4.2e-121 |
PFAM |
|
Pfam:Glyco_tran_28_C
|
326 |
437 |
6.4e-9 |
PFAM |
|
| Predicted Effect |
possibly damaging
Transcript: ENSMUST00000198610
AA Change: R322G
PolyPhen 2
Score 0.625 (Sensitivity: 0.87; Specificity: 0.91)
|
| SMART Domains |
Protein: ENSMUSP00000143605
Gene: ENSMUSG00000032854
AA Change: R322G
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:UDPGT
|
21 |
510 |
4.2e-121 |
PFAM |
|
Pfam:Glyco_tran_28_C
|
326 |
437 |
6.4e-9 |
PFAM |
|
| Meta Mutation Damage Score |
0.8691 |
| Coding Region Coverage |
- 1x: 0.0%
- 3x: 0.0%
- 10x: 0.0%
- 20x: 0.0%
|
| Validation Efficiency |
95% (41/43) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene belongs to the UDP-glycosyltransferase family. It catalyzes the transfer of galactose to ceramide, a key enzymatic step in the biosynthesis of galactocerebrosides, which are abundant sphingolipids of the myelin membrane of the central and peripheral nervous systems. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2011]
PHENOTYPE: Mutants fail to make galactolipid galactocerebroside and its sulfated derivative that are normal myelin constituents. Mutants have tremors, ataxia, progressive hindlimb paralysis and vacuole formation in ventral spinal cord due to abnormal myelin sheath. [provided by MGI curators]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 37 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| 4933416I08Rik |
TCC |
TCCC |
X: 52,692,862 (GRCm39) |
|
noncoding transcript |
Het |
| Ankrd2 |
G |
A |
19: 42,028,533 (GRCm39) |
R63H |
probably damaging |
Het |
| Bbs2 |
C |
A |
8: 94,801,041 (GRCm39) |
|
probably benign |
Het |
| Btbd7 |
G |
T |
12: 102,779,065 (GRCm39) |
D400E |
probably benign |
Het |
| Cilp |
TGGG |
TGG |
9: 65,187,412 (GRCm39) |
|
probably null |
Het |
| Col17a1 |
C |
T |
19: 47,636,537 (GRCm39) |
|
probably null |
Het |
| Col3a1 |
A |
G |
1: 45,368,085 (GRCm39) |
|
probably null |
Het |
| Crb1 |
CG |
C |
1: 139,164,824 (GRCm39) |
|
probably null |
Het |
| Dpp8 |
C |
G |
9: 64,962,118 (GRCm39) |
S386C |
probably benign |
Het |
| Dscaml1 |
A |
C |
9: 45,654,297 (GRCm39) |
D1443A |
probably damaging |
Het |
| Dsp |
T |
C |
13: 38,372,421 (GRCm39) |
|
probably benign |
Het |
| Elf2 |
C |
T |
3: 51,165,038 (GRCm39) |
R262Q |
probably benign |
Het |
| Fat3 |
G |
A |
9: 15,907,896 (GRCm39) |
S2702F |
probably benign |
Het |
| G530012D18Rik |
CAGAGAGA |
CAGAGAGAGA |
1: 85,504,945 (GRCm39) |
|
probably null |
Het |
| Gabrb1 |
T |
A |
5: 72,279,497 (GRCm39) |
S347R |
probably benign |
Het |
| Hivep1 |
T |
C |
13: 42,309,604 (GRCm39) |
S615P |
probably benign |
Het |
| Kcnq3 |
T |
C |
15: 65,897,027 (GRCm39) |
D291G |
possibly damaging |
Het |
| Lyl1 |
C |
T |
8: 85,429,300 (GRCm39) |
P3L |
possibly damaging |
Het |
| Mirt1 |
A |
G |
19: 53,433,710 (GRCm39) |
|
noncoding transcript |
Het |
| Mmp1a |
TG |
TGG |
9: 7,465,083 (GRCm38) |
|
probably null |
Het |
| Mug1 |
T |
A |
6: 121,857,530 (GRCm39) |
S1085T |
possibly damaging |
Het |
| Myo18a |
A |
T |
11: 77,709,596 (GRCm39) |
T190S |
probably benign |
Het |
| Myo5a |
G |
A |
9: 75,054,191 (GRCm39) |
|
probably null |
Het |
| Or1ak2 |
T |
A |
2: 36,827,635 (GRCm39) |
F168Y |
probably damaging |
Het |
| Or1l8 |
A |
T |
2: 36,817,820 (GRCm39) |
M102K |
probably damaging |
Het |
| Or1m1 |
A |
T |
9: 18,666,750 (GRCm39) |
Y60* |
probably null |
Het |
| Or5an1 |
G |
T |
19: 12,260,876 (GRCm39) |
V155L |
probably benign |
Het |
| Or9g3 |
A |
G |
2: 85,589,785 (GRCm39) |
*312Q |
probably null |
Het |
| Relch |
T |
A |
1: 105,654,106 (GRCm39) |
V825E |
probably damaging |
Het |
| Rgma |
G |
A |
7: 73,067,263 (GRCm39) |
V173M |
probably damaging |
Het |
| Rgsl1 |
G |
A |
1: 153,701,422 (GRCm39) |
S379F |
possibly damaging |
Het |
| Sec16a |
T |
A |
2: 26,305,759 (GRCm39) |
D2215V |
probably damaging |
Het |
| Speer4c1 |
A |
C |
5: 15,919,214 (GRCm39) |
|
probably benign |
Het |
| Thrap3 |
G |
A |
4: 126,059,335 (GRCm39) |
|
probably null |
Het |
| Ttyh2 |
T |
A |
11: 114,599,680 (GRCm39) |
L370Q |
probably damaging |
Het |
| Vmn2r97 |
T |
C |
17: 19,167,900 (GRCm39) |
M718T |
possibly damaging |
Het |
| Zhx2 |
T |
C |
15: 57,686,229 (GRCm39) |
F533L |
possibly damaging |
Het |
|
| Other mutations in Ugt8a |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00264:Ugt8a
|
APN |
3 |
125,708,285 (GRCm39) |
critical splice donor site |
probably null |
|
|
IGL01934:Ugt8a
|
APN |
3 |
125,708,424 (GRCm39) |
missense |
probably benign |
0.18 |
|
IGL02435:Ugt8a
|
APN |
3 |
125,660,969 (GRCm39) |
missense |
probably benign |
0.00 |
|
R0041:Ugt8a
|
UTSW |
3 |
125,708,739 (GRCm39) |
missense |
probably benign |
0.00 |
|
R0453:Ugt8a
|
UTSW |
3 |
125,708,606 (GRCm39) |
missense |
probably benign |
0.03 |
|
R1314:Ugt8a
|
UTSW |
3 |
125,665,397 (GRCm39) |
missense |
probably benign |
0.00 |
|
R1544:Ugt8a
|
UTSW |
3 |
125,709,098 (GRCm39) |
missense |
probably benign |
0.06 |
|
R1566:Ugt8a
|
UTSW |
3 |
125,669,207 (GRCm39) |
missense |
probably damaging |
0.96 |
|
R1770:Ugt8a
|
UTSW |
3 |
125,667,852 (GRCm39) |
missense |
probably benign |
0.11 |
|
R2126:Ugt8a
|
UTSW |
3 |
125,669,195 (GRCm39) |
missense |
probably damaging |
0.98 |
|
R2972:Ugt8a
|
UTSW |
3 |
125,708,957 (GRCm39) |
missense |
probably benign |
|
|
R2973:Ugt8a
|
UTSW |
3 |
125,708,957 (GRCm39) |
missense |
probably benign |
|
|
R3547:Ugt8a
|
UTSW |
3 |
125,661,031 (GRCm39) |
nonsense |
probably null |
|
|
R3906:Ugt8a
|
UTSW |
3 |
125,708,631 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R3907:Ugt8a
|
UTSW |
3 |
125,708,631 (GRCm39) |
missense |
possibly damaging |
0.95 |
|
R4032:Ugt8a
|
UTSW |
3 |
125,667,807 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5235:Ugt8a
|
UTSW |
3 |
125,661,129 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5890:Ugt8a
|
UTSW |
3 |
125,669,202 (GRCm39) |
missense |
probably benign |
0.01 |
|
R6790:Ugt8a
|
UTSW |
3 |
125,665,340 (GRCm39) |
missense |
possibly damaging |
0.93 |
|
R6937:Ugt8a
|
UTSW |
3 |
125,709,250 (GRCm39) |
start gained |
probably benign |
|
|
R7298:Ugt8a
|
UTSW |
3 |
125,709,065 (GRCm39) |
missense |
probably benign |
0.30 |
|
R8730:Ugt8a
|
UTSW |
3 |
125,732,105 (GRCm39) |
start gained |
probably benign |
|
|
R9211:Ugt8a
|
UTSW |
3 |
125,661,130 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9385:Ugt8a
|
UTSW |
3 |
125,665,263 (GRCm39) |
missense |
probably benign |
|
|
R9649:Ugt8a
|
UTSW |
3 |
125,708,338 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R9666:Ugt8a
|
UTSW |
3 |
125,708,957 (GRCm39) |
missense |
probably benign |
|
|
R9762:Ugt8a
|
UTSW |
3 |
125,708,900 (GRCm39) |
missense |
probably damaging |
0.98 |
|
| Predicted Primers |
PCR Primer
(F):5'- GCTGGAGGAAACATAAAACTGACTC -3'
(R):5'- GCACTGACTTGTATTGGTCTTC -3'
Sequencing Primer
(F):5'- TACAGGTATCTTATCAACTACAGACC -3'
(R):5'- AACATGGCTTTGTCCTGG -3'
|
| Posted On |
2017-02-08 |
Incidental Mutation