Mutagenetix > Incidental Mutation

Incidental Mutation 'R5864:Gm5174'

454073

Institutional Source

Beutler Lab

Gene Symbol

Gm5174

Ensembl Gene

ENSMUSG00000090308

Gene Name

predicted gene 5174

Synonyms

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.108) question?

Stock #

R5864 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

86655939-86657381 bp(+) (GRCm38)

Type of Mutation

unclassified

DNA Base Change (assembly)

T to A at 86657181 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s):

AlphaFold

no structure available at present

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000171131

SMART Domains

Protein: ENSMUSP00000129952
Gene: ENSMUSG00000090308

Domain	Start	End	E-Value	Type
S_TKc	14	262	5.59e-86	SMART
UBA	276	313	1.28e-3	SMART
low complexity region	447	462	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219608

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 56 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Acot4	A	T	12: 84,043,404	I292F	probably benign	Het
Adamdec1	A	G	14: 68,570,102	S370P	probably damaging	Het
Ankar	T	C	1: 72,659,165	K692R	probably benign	Het
Ano6	T	C	15: 95,920,380		probably null	Het
Apopt1	G	A	12: 111,751,218	V171I	probably benign	Het
Asns	G	A	6: 7,675,443	Q520*	probably null	Het
Bbs12	C	T	3: 37,319,490	T144I	probably damaging	Het
BC030867	G	A	11: 102,255,146	E83K	probably benign	Het
C8g	C	T	2: 25,498,943	G186D	probably damaging	Het
Clptm1l	T	C	13: 73,606,284	F109S	probably damaging	Het
Col4a1	G	A	8: 11,202,973		probably benign	Het
Cpn2	T	C	16: 30,259,683	D400G	probably damaging	Het
Dgke	G	C	11: 89,050,462	Y298*	probably null	Het
Dnah5	G	A	15: 28,297,013	R1451Q	possibly damaging	Het
Dock8	A	G	19: 25,061,220	D90G	probably damaging	Het
Dok7	A	C	5: 35,066,546	D143A	probably damaging	Het
Elk3	G	A	10: 93,284,791	A62V	probably damaging	Het
Epha2	T	A	4: 141,308,427	M58K	probably damaging	Het
Erp27	G	T	6: 136,908,100	D233E	probably benign	Het
Gxylt2	A	G	6: 100,783,146	D214G	probably damaging	Het
Ifi35	A	T	11: 101,458,243	I238F	probably damaging	Het
Ighmbp2	T	C	19: 3,261,467	T983A	probably benign	Het
Itgb4	C	T	11: 115,990,922	R766W	probably damaging	Het
Lrp1	C	A	10: 127,567,505	K2066N	possibly damaging	Het
Mansc1	T	G	6: 134,610,853		probably null	Het
Mapre3	T	C	5: 30,863,238	F101S	probably damaging	Het
Mettl8	T	C	2: 70,982,013	T58A	probably benign	Het
Mical1	G	T	10: 41,486,068	R857L	possibly damaging	Het
Nacad	T	C	11: 6,600,581	D870G	probably benign	Het
Nectin1	A	G	9: 43,791,310	D118G	probably damaging	Het
Nlrp2	A	T	7: 5,322,381	L26Q	probably damaging	Het
Olfr512	A	G	7: 108,713,464	D25G	probably benign	Het
Pamr1	T	C	2: 102,634,348	S281P	possibly damaging	Het
Pcdhgc5	T	A	18: 37,821,761	V696E	probably damaging	Het
Pde4d	G	T	13: 109,938,048	A396S	probably benign	Het
Pecam1	G	T	11: 106,684,250	C510*	probably null	Het
Pga5	C	T	19: 10,675,149	G76S	probably damaging	Het
Phldb3	A	T	7: 24,624,146	H435L	possibly damaging	Het
Piezo1	T	C	8: 122,486,373	R1884G	possibly damaging	Het
Ripk4	T	C	16: 97,763,582	H43R	probably damaging	Het
Rtn3	A	G	19: 7,435,111	V785A	probably damaging	Het
Safb2	A	G	17: 56,566,491		probably benign	Het
Sephs1	T	C	2: 4,905,582	F288L	probably damaging	Het
Sez6l	A	G	5: 112,438,400		probably null	Het
Siglece	A	G	7: 43,659,317	L204P	probably damaging	Het
Sis	T	C	3: 72,949,818	D380G	probably damaging	Het
Slamf9	G	T	1: 172,476,466	R126L	probably benign	Het
Sorl1	A	G	9: 42,092,373	L209P	probably damaging	Het
Sptbn1	A	C	11: 30,145,925	I310S	probably damaging	Het
Tex52	A	G	6: 128,379,682	T113A	probably benign	Het
Trav6-3	T	A	14: 53,430,171	Y33*	probably null	Het
Vmn2r102	A	G	17: 19,694,681	E836G	possibly damaging	Het
Wdsub1	C	T	2: 59,878,475	C18Y	probably damaging	Het
Zfp341	C	A	2: 154,643,554	H637N	possibly damaging	Het
Zfp445	C	T	9: 122,853,487	S463N	probably benign	Het
Zfp612	G	A	8: 110,089,726	D522N	probably damaging	Het

Other mutations in Gm5174

Allele	Source	Chr	Coord	Type	Predicted Effect
Laco	UTSW	10	86656108	unclassified	noncoding transcript
R1083:Gm5174	UTSW	10	86656108	unclassified	noncoding transcript
R1199:Gm5174	UTSW	10	86657325	unclassified	noncoding transcript
R1296:Gm5174	UTSW	10	86657002	unclassified	noncoding transcript
R1715:Gm5174	UTSW	10	86656912	unclassified	noncoding transcript
R1957:Gm5174	UTSW	10	86656753	unclassified	noncoding transcript
R2221:Gm5174	UTSW	10	86656508	unclassified	noncoding transcript
R2223:Gm5174	UTSW	10	86656508	unclassified	noncoding transcript
R3104:Gm5174	UTSW	10	86656655	unclassified	noncoding transcript
R4165:Gm5174	UTSW	10	86656933	unclassified	noncoding transcript
R4166:Gm5174	UTSW	10	86656933	unclassified	noncoding transcript
R4243:Gm5174	UTSW	10	86656280	unclassified	noncoding transcript
R4244:Gm5174	UTSW	10	86656280	unclassified	noncoding transcript
R5024:Gm5174	UTSW	10	86656587	unclassified	noncoding transcript
R5292:Gm5174	UTSW	10	86656698	unclassified	noncoding transcript
R5586:Gm5174	UTSW	10	86656545	unclassified	noncoding transcript

Predicted Primers

PCR Primer
(F):5'- TCTTCCCCAAAAGAGAAGGGC -3'
(R):5'- CCACTTTCTTGGAGGATCTACGC -3'

Sequencing Primer
(F):5'- GGCCAGTGAACCCAACTTTG -3'
(R):5'- ATCTACGCTGGGTGTTTGTCTC -3'

Posted On

2017-02-10