Incidental Mutation 'R5855:Klra7'
ID454866
Institutional Source Beutler Lab
Gene Symbol Klra7
Ensembl Gene ENSMUSG00000067599
Gene Namekiller cell lectin-like receptor, subfamily A, member 7
SynonymsLy-49G.1, Ly-49G.2, Ly-49G.3, Ly49g2, Ly49g1, LGL-1, Ly49g
MMRRC Submission 043229-MU
Accession Numbers
Is this an essential gene? Non essential (E-score: 0.000) question?
Stock #R5855 (G1)
Quality Score225
Status Not validated
Chromosome6
Chromosomal Location130218605-130233322 bp(-) (GRCm38)
Type of Mutationmissense
DNA Base Change (assembly) T to C at 130218958 bp
ZygosityHeterozygous
Amino Acid Change Aspartic acid to Glycine at position 262 (D262G)
Ref Sequence ENSEMBL: ENSMUSP00000107644 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000032286] [ENSMUST00000049304] [ENSMUST00000088011] [ENSMUST00000112013]
Predicted Effect possibly damaging
Transcript: ENSMUST00000032286
AA Change: D262G

PolyPhen 2 Score 0.750 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000032286
Gene: ENSMUSG00000067599
AA Change: D262G

DomainStartEndE-ValueType
low complexity region 56 68 N/A INTRINSIC
Blast:CLECT 73 123 9e-22 BLAST
CLECT 157 272 3.09e-16 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000049304
AA Change: D249G

PolyPhen 2 Score 0.375 (Sensitivity: 0.90; Specificity: 0.89)
SMART Domains Protein: ENSMUSP00000037917
Gene: ENSMUSG00000067599
AA Change: D249G

DomainStartEndE-ValueType
low complexity region 56 68 N/A INTRINSIC
Blast:CLECT 73 123 7e-22 BLAST
CLECT 144 259 3.09e-16 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000088011
AA Change: D249G

PolyPhen 2 Score 0.227 (Sensitivity: 0.91; Specificity: 0.88)
SMART Domains Protein: ENSMUSP00000085326
Gene: ENSMUSG00000067599
AA Change: D249G

DomainStartEndE-ValueType
low complexity region 56 68 N/A INTRINSIC
Blast:CLECT 73 123 7e-22 BLAST
CLECT 144 259 3.09e-16 SMART
Predicted Effect possibly damaging
Transcript: ENSMUST00000112013
AA Change: D262G

PolyPhen 2 Score 0.750 (Sensitivity: 0.85; Specificity: 0.92)
SMART Domains Protein: ENSMUSP00000107644
Gene: ENSMUSG00000067599
AA Change: D262G

DomainStartEndE-ValueType
low complexity region 56 68 N/A INTRINSIC
Blast:CLECT 73 123 9e-22 BLAST
CLECT 157 272 3.09e-16 SMART
Coding Region Coverage
  • 1x: 99.8%
  • 3x: 99.4%
  • 10x: 96.8%
  • 20x: 89.3%
Validation Efficiency
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Acadsb T G 7: 131,424,599 L57R probably damaging Het
Bmpr1b G A 3: 141,871,385 T55M possibly damaging Het
Cep350 G A 1: 155,953,762 T132I probably benign Het
Cops4 A T 5: 100,547,414 M400L probably benign Het
Cul1 G A 6: 47,523,213 D653N probably benign Het
Cyp3a13 C G 5: 137,919,056 L36F probably damaging Het
Dcaf10 T C 4: 45,342,558 F131L probably benign Het
Dgkh T C 14: 78,624,504 probably null Het
Igll1 C T 16: 16,861,057 V130M probably damaging Het
Kif2c C T 4: 117,182,542 probably benign Het
Lrrc27 T C 7: 139,218,335 probably benign Het
Maf A G 8: 115,705,792 S358P probably benign Het
Map1a A G 2: 121,303,674 D1419G possibly damaging Het
Map3k1 G A 13: 111,755,979 A914V probably benign Het
Naa25 T G 5: 121,423,692 L436R possibly damaging Het
Ndc1 T C 4: 107,383,707 I294T probably damaging Het
Nek1 A C 8: 61,016,272 D121A probably damaging Het
Nfil3 C T 13: 52,968,710 G53R probably benign Het
Olfr63 T C 17: 33,269,336 V204A possibly damaging Het
Parp14 A T 16: 35,840,927 Y1550* probably null Het
Patl1 A G 19: 11,921,516 I192V probably damaging Het
Pax3 G A 1: 78,121,651 T367I probably damaging Het
Pla2g4a C T 1: 149,880,063 V208M probably damaging Het
Prdm10 A T 9: 31,337,323 K347M probably damaging Het
Prkd1 A T 12: 50,392,916 M376K probably benign Het
Prkg1 C T 19: 30,894,694 V219I possibly damaging Het
Rictor G A 15: 6,794,006 E1555K probably benign Het
Scn3a T C 2: 65,464,730 I1550V possibly damaging Het
Skint8 C A 4: 111,950,193 L359M probably damaging Het
Sox4 T C 13: 28,952,996 E9G probably damaging Het
Spon1 T A 7: 114,029,072 D354E probably damaging Het
Stat2 T A 10: 128,283,494 L450H probably damaging Het
Tek T A 4: 94,853,553 M849K probably damaging Het
Tmem63c G A 12: 87,075,726 D433N probably damaging Het
Tnpo3 G A 6: 29,589,033 T106I probably damaging Het
Tns1 G T 1: 73,918,033 A1674D possibly damaging Het
Trim8 T C 19: 46,515,410 V467A possibly damaging Het
Trmo T A 4: 46,382,568 H183L probably benign Het
Trpm1 C A 7: 64,268,962 C683* probably null Het
Vsig10 T G 5: 117,338,270 L263R probably damaging Het
Zfp874a A T 13: 67,442,693 Y291N probably benign Het
Other mutations in Klra7
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01369:Klra7 APN 6 130226535 nonsense probably null
IGL02708:Klra7 APN 6 130226500 missense probably damaging 1.00
R0890:Klra7 UTSW 6 130218953 missense probably benign 0.00
R1566:Klra7 UTSW 6 130231601 missense probably damaging 0.98
R1815:Klra7 UTSW 6 130224107 missense probably benign 0.23
R1843:Klra7 UTSW 6 130229994 missense possibly damaging 0.79
R2216:Klra7 UTSW 6 130228586 missense probably benign 0.18
R2313:Klra7 UTSW 6 130228542 missense probably benign 0.04
R4067:Klra7 UTSW 6 130231649 splice site probably null
R6651:Klra7 UTSW 6 130229945 missense probably benign 0.40
R7508:Klra7 UTSW 6 130230091 splice site probably null
R8445:Klra7 UTSW 6 130227115 missense possibly damaging 0.86
R8458:Klra7 UTSW 6 130224146 missense probably damaging 1.00
Predicted Primers PCR Primer
(F):5'- GCACTGCAGTAAGTCCAATGG -3'
(R):5'- TGTGTGACAGAGATCGTTTCA -3'

Sequencing Primer
(F):5'- TGCAGTAAGTCCAATGGTCAAAAC -3'
(R):5'- GTGACAGAGATCGTTTCATTTTAATG -3'
Posted On2017-02-10