Incidental Mutation 'R5855:Zfp874a'
| ID |
454880 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Zfp874a
|
| Ensembl Gene |
ENSMUSG00000069206 |
| Gene Name |
zinc finger protein 874a |
| Synonyms |
C330011K17Rik, Zfp874, Rslcan15 |
| MMRRC Submission |
043229-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably non essential
(E-score: 0.078)
|
| Stock # |
R5855 (G1)
|
| Quality Score |
225 |
|
Status
|
Not validated
|
| Chromosome |
13 |
| Chromosomal Location |
67588550-67599743 bp(-) (GRCm39) |
| Type of Mutation |
missense |
| DNA Base Change (assembly) |
A to T
at 67590812 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Asparagine
at position 291
(Y291N)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000074735
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000057241]
[ENSMUST00000075255]
[ENSMUST00000180580]
[ENSMUST00000223682]
[ENSMUST00000225479]
|
| AlphaFold |
Q8BX23 |
| Predicted Effect |
probably benign
Transcript: ENSMUST00000057241
|
| SMART Domains |
Protein: ENSMUSP00000077307
Gene: ENSMUSG00000069206
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
1.73e-27 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000075255
AA Change: Y291N
PolyPhen 2
Score 0.028 (Sensitivity: 0.95; Specificity: 0.81)
|
| SMART Domains |
Protein: ENSMUSP00000074735
Gene: ENSMUSG00000069206
AA Change: Y291N
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
1.73e-27 |
SMART |
|
ZnF_C2H2
|
137 |
159 |
9.58e-3 |
SMART |
|
ZnF_C2H2
|
165 |
187 |
5.99e-4 |
SMART |
|
ZnF_C2H2
|
193 |
215 |
7.26e-3 |
SMART |
|
ZnF_C2H2
|
221 |
243 |
2.4e-3 |
SMART |
|
ZnF_C2H2
|
249 |
269 |
1.88e2 |
SMART |
|
ZnF_C2H2
|
277 |
299 |
5.21e-4 |
SMART |
|
ZnF_C2H2
|
305 |
327 |
2.12e-4 |
SMART |
|
ZnF_C2H2
|
333 |
355 |
2.12e-4 |
SMART |
|
ZnF_C2H2
|
361 |
383 |
8.47e-4 |
SMART |
|
ZnF_C2H2
|
389 |
411 |
1.79e-2 |
SMART |
|
ZnF_C2H2
|
417 |
438 |
2.86e-1 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000180580
|
| SMART Domains |
Protein: ENSMUSP00000137953
Gene: ENSMUSG00000069206
| Domain | Start | End | E-Value | Type |
|---|
|
KRAB
|
5 |
65 |
1.73e-27 |
SMART |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000223682
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000224239
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000225479
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000225778
|
| Coding Region Coverage |
- 1x: 99.8%
- 3x: 99.4%
- 10x: 96.8%
- 20x: 89.3%
|
| Validation Efficiency |
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 41 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Acadsb |
T |
G |
7: 131,026,328 (GRCm39) |
L57R |
probably damaging |
Het |
| Bmpr1b |
G |
A |
3: 141,577,146 (GRCm39) |
T55M |
possibly damaging |
Het |
| Cep350 |
G |
A |
1: 155,829,508 (GRCm39) |
T132I |
probably benign |
Het |
| Cops4 |
A |
T |
5: 100,695,280 (GRCm39) |
M400L |
probably benign |
Het |
| Cul1 |
G |
A |
6: 47,500,147 (GRCm39) |
D653N |
probably benign |
Het |
| Cyp3a13 |
C |
G |
5: 137,917,318 (GRCm39) |
L36F |
probably damaging |
Het |
| Dcaf10 |
T |
C |
4: 45,342,558 (GRCm39) |
F131L |
probably benign |
Het |
| Dgkh |
T |
C |
14: 78,861,944 (GRCm39) |
|
probably null |
Het |
| Igll1 |
C |
T |
16: 16,678,921 (GRCm39) |
V130M |
probably damaging |
Het |
| Kif2c |
C |
T |
4: 117,039,739 (GRCm39) |
|
probably benign |
Het |
| Klra7 |
T |
C |
6: 130,195,921 (GRCm39) |
D262G |
possibly damaging |
Het |
| Lrrc27 |
T |
C |
7: 138,798,251 (GRCm39) |
|
probably benign |
Het |
| Maf |
A |
G |
8: 116,432,531 (GRCm39) |
S358P |
probably benign |
Het |
| Map1a |
A |
G |
2: 121,134,155 (GRCm39) |
D1419G |
possibly damaging |
Het |
| Map3k1 |
G |
A |
13: 111,892,513 (GRCm39) |
A914V |
probably benign |
Het |
| Naa25 |
T |
G |
5: 121,561,755 (GRCm39) |
L436R |
possibly damaging |
Het |
| Ndc1 |
T |
C |
4: 107,240,904 (GRCm39) |
I294T |
probably damaging |
Het |
| Nek1 |
A |
C |
8: 61,469,306 (GRCm39) |
D121A |
probably damaging |
Het |
| Nfil3 |
C |
T |
13: 53,122,746 (GRCm39) |
G53R |
probably benign |
Het |
| Or10h28 |
T |
C |
17: 33,488,310 (GRCm39) |
V204A |
possibly damaging |
Het |
| Parp14 |
A |
T |
16: 35,661,297 (GRCm39) |
Y1550* |
probably null |
Het |
| Patl1 |
A |
G |
19: 11,898,880 (GRCm39) |
I192V |
probably damaging |
Het |
| Pax3 |
G |
A |
1: 78,098,288 (GRCm39) |
T367I |
probably damaging |
Het |
| Pla2g4a |
C |
T |
1: 149,755,814 (GRCm39) |
V208M |
probably damaging |
Het |
| Prdm10 |
A |
T |
9: 31,248,619 (GRCm39) |
K347M |
probably damaging |
Het |
| Prkd1 |
A |
T |
12: 50,439,699 (GRCm39) |
M376K |
probably benign |
Het |
| Prkg1 |
C |
T |
19: 30,872,094 (GRCm39) |
V219I |
possibly damaging |
Het |
| Rictor |
G |
A |
15: 6,823,487 (GRCm39) |
E1555K |
probably benign |
Het |
| Scn3a |
T |
C |
2: 65,295,074 (GRCm39) |
I1550V |
possibly damaging |
Het |
| Skint8 |
C |
A |
4: 111,807,390 (GRCm39) |
L359M |
probably damaging |
Het |
| Sox4 |
T |
C |
13: 29,136,979 (GRCm39) |
E9G |
probably damaging |
Het |
| Spon1 |
T |
A |
7: 113,628,307 (GRCm39) |
D354E |
probably damaging |
Het |
| Stat2 |
T |
A |
10: 128,119,363 (GRCm39) |
L450H |
probably damaging |
Het |
| Tek |
T |
A |
4: 94,741,790 (GRCm39) |
M849K |
probably damaging |
Het |
| Tmem63c |
G |
A |
12: 87,122,500 (GRCm39) |
D433N |
probably damaging |
Het |
| Tnpo3 |
G |
A |
6: 29,589,032 (GRCm39) |
T106I |
probably damaging |
Het |
| Tns1 |
G |
T |
1: 73,957,192 (GRCm39) |
A1674D |
possibly damaging |
Het |
| Trim8 |
T |
C |
19: 46,503,849 (GRCm39) |
V467A |
possibly damaging |
Het |
| Trmo |
T |
A |
4: 46,382,568 (GRCm39) |
H183L |
probably benign |
Het |
| Trpm1 |
C |
A |
7: 63,918,710 (GRCm39) |
C683* |
probably null |
Het |
| Vsig10 |
T |
G |
5: 117,476,335 (GRCm39) |
L263R |
probably damaging |
Het |
|
| Other mutations in Zfp874a |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL02490:Zfp874a
|
APN |
13 |
67,590,819 (GRCm39) |
nonsense |
probably null |
|
|
R1052:Zfp874a
|
UTSW |
13 |
67,590,539 (GRCm39) |
missense |
possibly damaging |
0.89 |
|
R1991:Zfp874a
|
UTSW |
13 |
67,590,623 (GRCm39) |
missense |
probably benign |
0.03 |
|
R2085:Zfp874a
|
UTSW |
13 |
67,590,967 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R2103:Zfp874a
|
UTSW |
13 |
67,590,623 (GRCm39) |
missense |
probably benign |
0.03 |
|
R5129:Zfp874a
|
UTSW |
13 |
67,591,100 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5336:Zfp874a
|
UTSW |
13 |
67,590,799 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R5423:Zfp874a
|
UTSW |
13 |
67,590,473 (GRCm39) |
missense |
possibly damaging |
0.75 |
|
R5713:Zfp874a
|
UTSW |
13 |
67,597,476 (GRCm39) |
missense |
probably benign |
0.16 |
|
R6496:Zfp874a
|
UTSW |
13 |
67,590,694 (GRCm39) |
missense |
possibly damaging |
0.62 |
|
R7046:Zfp874a
|
UTSW |
13 |
67,590,418 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R7065:Zfp874a
|
UTSW |
13 |
67,590,401 (GRCm39) |
missense |
probably damaging |
0.97 |
|
R7233:Zfp874a
|
UTSW |
13 |
67,590,776 (GRCm39) |
missense |
possibly damaging |
0.83 |
|
R7271:Zfp874a
|
UTSW |
13 |
67,591,415 (GRCm39) |
missense |
probably benign |
0.00 |
|
R7465:Zfp874a
|
UTSW |
13 |
67,590,376 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R7468:Zfp874a
|
UTSW |
13 |
67,573,723 (GRCm39) |
splice site |
probably null |
|
|
R8108:Zfp874a
|
UTSW |
13 |
67,591,353 (GRCm39) |
nonsense |
probably null |
|
|
R8109:Zfp874a
|
UTSW |
13 |
67,597,563 (GRCm39) |
missense |
possibly damaging |
0.46 |
|
R8843:Zfp874a
|
UTSW |
13 |
67,590,764 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8859:Zfp874a
|
UTSW |
13 |
67,590,647 (GRCm39) |
missense |
probably benign |
0.17 |
|
R9137:Zfp874a
|
UTSW |
13 |
67,590,841 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R9673:Zfp874a
|
UTSW |
13 |
67,591,156 (GRCm39) |
missense |
probably benign |
0.03 |
|
X0063:Zfp874a
|
UTSW |
13 |
67,591,106 (GRCm39) |
missense |
probably damaging |
1.00 |
|
Z1177:Zfp874a
|
UTSW |
13 |
67,590,781 (GRCm39) |
missense |
probably damaging |
1.00 |
|
| Predicted Primers |
PCR Primer
(F):5'- AATGGATTTGATGTCTTTTCAGGGAT -3'
(R):5'- CTCTTAGTAGTCGCTCATCACTT -3'
Sequencing Primer
(F):5'- GCATTCTACACACTCATGGGG -3'
(R):5'- GAGTGTGACAAAGCCTTTCTTC -3'
|
| Posted On |
2017-02-10 |
Incidental Mutation