Mutagenetix > Incidental Mutations

Incidental Mutation 'R0555:Asap1'

45513

Institutional Source

Beutler Lab

Gene Symbol

Asap1

Ensembl Gene

ENSMUSG00000022377

Gene Name

ArfGAP with SH3 domain, ankyrin repeat and PH domain1

Synonyms

Ddef1, mKIAA1249

MMRRC Submission

038747-MU

Accession Numbers

Genbank: NM_010026; MGI: 1342335

Is this an essential gene?

Probably non essential (E-score: 0.133) question?

Stock #

R0555 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64086857-64382919 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 64094364 bp

Zygosity

Heterozygous

Amino Acid Change

Leucine to Proline at position 941 (L941P)

Ref Sequence

ENSEMBL: ENSMUSP00000135643 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000023008] [ENSMUST00000110114] [ENSMUST00000110115] [ENSMUST00000175793] [ENSMUST00000175799] [ENSMUST00000176014] [ENSMUST00000176384] [ENSMUST00000177035] [ENSMUST00000177083] [ENSMUST00000177371] [ENSMUST00000177374]

Predicted Effect

probably damaging
Transcript: ENSMUST00000023008
AA Change: L964P

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000023008
Gene: ENSMUSG00000022377
AA Change: L964P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
PH	340	433	4.12e-15	SMART
ArfGap	454	577	2.18e-34	SMART
ANK	615	647	1.17e-1	SMART
ANK	651	680	3.46e-4	SMART
low complexity region	727	738	N/A	INTRINSIC
low complexity region	792	803	N/A	INTRINSIC
low complexity region	814	847	N/A	INTRINSIC
low complexity region	856	865	N/A	INTRINSIC
low complexity region	892	903	N/A	INTRINSIC
low complexity region	971	984	N/A	INTRINSIC
low complexity region	1065	1077	N/A	INTRINSIC
SH3	1088	1146	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000110114
AA Change: L907P

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000105741
Gene: ENSMUSG00000022377
AA Change: L907P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
PH	340	433	4.12e-15	SMART
ArfGap	454	577	2.18e-34	SMART
ANK	615	647	1.17e-1	SMART
ANK	651	680	3.46e-4	SMART
low complexity region	727	738	N/A	INTRINSIC
low complexity region	792	803	N/A	INTRINSIC
low complexity region	835	846	N/A	INTRINSIC
low complexity region	914	927	N/A	INTRINSIC
low complexity region	1008	1020	N/A	INTRINSIC
SH3	1031	1089	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000110115
AA Change: L949P

PolyPhen 2 Score 0.981 (Sensitivity: 0.75; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000105742
Gene: ENSMUSG00000022377
AA Change: L949P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
PH	325	418	4.12e-15	SMART
ArfGap	439	562	2.18e-34	SMART
ANK	600	632	1.17e-1	SMART
ANK	636	665	3.46e-4	SMART
low complexity region	712	723	N/A	INTRINSIC
low complexity region	777	788	N/A	INTRINSIC
low complexity region	799	832	N/A	INTRINSIC
low complexity region	841	850	N/A	INTRINSIC
low complexity region	877	888	N/A	INTRINSIC
low complexity region	956	969	N/A	INTRINSIC
low complexity region	1050	1062	N/A	INTRINSIC
SH3	1073	1131	3.29e-17	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000175793
AA Change: L952P

PolyPhen 2 Score 0.660 (Sensitivity: 0.86; Specificity: 0.91)

SMART Domains

Protein: ENSMUSP00000135718
Gene: ENSMUSG00000022377
AA Change: L952P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
PH	328	421	4.12e-15	SMART
ArfGap	442	565	2.18e-34	SMART
ANK	603	635	1.17e-1	SMART
ANK	639	668	3.46e-4	SMART
low complexity region	715	726	N/A	INTRINSIC
low complexity region	780	791	N/A	INTRINSIC
low complexity region	802	835	N/A	INTRINSIC
low complexity region	844	853	N/A	INTRINSIC
low complexity region	880	891	N/A	INTRINSIC
low complexity region	959	972	N/A	INTRINSIC
low complexity region	1053	1065	N/A	INTRINSIC
SH3	1076	1134	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000175799
AA Change: L904P

PolyPhen 2 Score 0.990 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000135359
Gene: ENSMUSG00000022377
AA Change: L904P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
PH	337	430	4.12e-15	SMART
ArfGap	451	574	2.18e-34	SMART
ANK	612	644	1.17e-1	SMART
ANK	648	677	3.46e-4	SMART
low complexity region	724	735	N/A	INTRINSIC
low complexity region	789	800	N/A	INTRINSIC
low complexity region	832	843	N/A	INTRINSIC
low complexity region	911	924	N/A	INTRINSIC
low complexity region	1005	1017	N/A	INTRINSIC
SH3	1028	1086	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000176014
AA Change: L961P

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000135172
Gene: ENSMUSG00000022377
AA Change: L961P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
PH	337	430	4.12e-15	SMART
ArfGap	451	574	2.18e-34	SMART
ANK	612	644	1.17e-1	SMART
ANK	648	677	3.46e-4	SMART
low complexity region	724	735	N/A	INTRINSIC
low complexity region	789	800	N/A	INTRINSIC
low complexity region	811	844	N/A	INTRINSIC
low complexity region	853	862	N/A	INTRINSIC
low complexity region	889	900	N/A	INTRINSIC
low complexity region	968	981	N/A	INTRINSIC
low complexity region	1062	1074	N/A	INTRINSIC
SH3	1085	1143	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000176384
AA Change: L907P

PolyPhen 2 Score 0.984 (Sensitivity: 0.74; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000135190
Gene: ENSMUSG00000022377
AA Change: L907P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
low complexity region	310	328	N/A	INTRINSIC
PH	340	433	4.12e-15	SMART
ArfGap	454	577	2.18e-34	SMART
ANK	615	647	1.17e-1	SMART
ANK	651	680	3.46e-4	SMART
low complexity region	727	738	N/A	INTRINSIC
low complexity region	792	803	N/A	INTRINSIC
low complexity region	835	846	N/A	INTRINSIC
low complexity region	914	927	N/A	INTRINSIC
low complexity region	1008	1020	N/A	INTRINSIC
SH3	1031	1089	3.29e-17	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176762

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176909

Predicted Effect

probably damaging
Transcript: ENSMUST00000177035
AA Change: L892P

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000135346
Gene: ENSMUSG00000022377
AA Change: L892P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
low complexity region	122	136	N/A	INTRINSIC
low complexity region	147	164	N/A	INTRINSIC
PH	325	418	4.12e-15	SMART
ArfGap	439	562	2.18e-34	SMART
ANK	600	632	1.17e-1	SMART
ANK	636	665	3.46e-4	SMART
low complexity region	712	723	N/A	INTRINSIC
low complexity region	777	788	N/A	INTRINSIC
low complexity region	820	831	N/A	INTRINSIC
low complexity region	899	912	N/A	INTRINSIC
low complexity region	993	1005	N/A	INTRINSIC
SH3	1016	1074	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000177083
AA Change: L929P

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000134877
Gene: ENSMUSG00000022377
AA Change: L929P

Domain	Start	End	E-Value	Type
low complexity region	102	116	N/A	INTRINSIC
low complexity region	127	144	N/A	INTRINSIC
PH	305	398	4.12e-15	SMART
ArfGap	419	542	2.18e-34	SMART
ANK	580	612	1.17e-1	SMART
ANK	616	645	3.46e-4	SMART
low complexity region	692	703	N/A	INTRINSIC
low complexity region	757	768	N/A	INTRINSIC
low complexity region	779	812	N/A	INTRINSIC
low complexity region	821	830	N/A	INTRINSIC
low complexity region	857	868	N/A	INTRINSIC
low complexity region	936	949	N/A	INTRINSIC
low complexity region	1030	1042	N/A	INTRINSIC
SH3	1053	1111	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000177371
AA Change: L941P

PolyPhen 2 Score 0.997 (Sensitivity: 0.41; Specificity: 0.98)

SMART Domains

Protein: ENSMUSP00000135643
Gene: ENSMUSG00000022377
AA Change: L941P

Domain	Start	End	E-Value	Type
low complexity region	102	116	N/A	INTRINSIC
low complexity region	127	144	N/A	INTRINSIC
PH	317	410	4.12e-15	SMART
ArfGap	431	554	2.18e-34	SMART
ANK	592	624	1.17e-1	SMART
ANK	628	657	3.46e-4	SMART
low complexity region	704	715	N/A	INTRINSIC
low complexity region	769	780	N/A	INTRINSIC
low complexity region	791	824	N/A	INTRINSIC
low complexity region	833	842	N/A	INTRINSIC
low complexity region	869	880	N/A	INTRINSIC
low complexity region	948	961	N/A	INTRINSIC
low complexity region	1042	1054	N/A	INTRINSIC
SH3	1065	1123	3.29e-17	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000177374
AA Change: L964P

PolyPhen 2 Score 0.994 (Sensitivity: 0.69; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000134825
Gene: ENSMUSG00000022377
AA Change: L964P

Domain	Start	End	E-Value	Type
low complexity region	2	16	N/A	INTRINSIC
Pfam:BAR	18	267	1.8e-11	PFAM
Pfam:BAR_3	52	286	1.2e-29	PFAM
low complexity region	310	328	N/A	INTRINSIC
PH	340	433	4.12e-15	SMART
ArfGap	454	577	2.18e-34	SMART
ANK	615	647	1.17e-1	SMART
ANK	651	680	3.46e-4	SMART
low complexity region	727	738	N/A	INTRINSIC
low complexity region	792	803	N/A	INTRINSIC
low complexity region	814	847	N/A	INTRINSIC
low complexity region	856	865	N/A	INTRINSIC
low complexity region	892	903	N/A	INTRINSIC
low complexity region	971	984	N/A	INTRINSIC
low complexity region	1065	1077	N/A	INTRINSIC
SH3	1088	1146	3.29e-17	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000228817

Meta Mutation Damage Score

0.1247

Coding Region Coverage

1x: 99.6%
3x: 98.8%
10x: 96.9%
20x: 93.8%

Validation Efficiency

100% (98/98)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes an ADP-ribosylation factor (ARF) GTPase-activating protein. The GTPase-activating activity is stimulated by phosphatidylinositol 4,5-biphosphate (PIP2), and is greater towards ARF1 and ARF5, and lesser for ARF6. This gene maybe involved in regulation of membrane trafficking and cytoskeleton remodeling. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Allele List at MGI

All alleles(13) : Gene trapped(13)

Other mutations in this stock

Total: 96 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam10	T	A	9: 70,754,234	I363N	probably damaging	Het
Ahcyl2	T	C	6: 29,890,671		probably benign	Het
Aurka	G	A	2: 172,367,147	R23C	probably benign	Het
Cacna1c	C	T	6: 118,612,625	R1446H	probably damaging	Het
Casp8ap2	T	A	4: 32,640,381	H478Q	probably damaging	Het
Clcn4	C	T	7: 7,290,504	A418T	possibly damaging	Het
Cpxm2	A	T	7: 132,044,043	Y715*	probably null	Het
Csmd1	T	C	8: 16,185,273	M1179V	probably benign	Het
Ddx21	A	T	10: 62,587,528	F632I	probably damaging	Het
Dnaic1	C	A	4: 41,625,335	T433K	possibly damaging	Het
Dpyd	G	A	3: 119,431,542	G988D	probably damaging	Het
Dync1i2	AAAAGAAGAGGAAAGAAGAGGAAAG	AAAAGAAGAGGAAAG	2: 71,214,518		probably null	Het
Dync1li2	A	T	8: 104,420,665	S466T	probably benign	Het
Ears2	T	C	7: 122,048,444	T206A	probably benign	Het
Elmod1	A	T	9: 53,931,592		probably benign	Het
Eps8l3	C	A	3: 107,892,345	D590E	probably benign	Het
Etfdh	A	T	3: 79,605,805	H370Q	probably benign	Het
Fam83g	C	A	11: 61,707,663	A792E	probably benign	Het
Ffar3	G	T	7: 30,855,537	Y119*	probably null	Het
Fosb	G	T	7: 19,307,213	S118R	possibly damaging	Het
Foxn4	G	A	5: 114,263,114	L3F	probably damaging	Het
Foxo4	A	G	X: 101,255,178	K65E	probably damaging	Het
Frem2	A	G	3: 53,516,860	L3052P	probably damaging	Het
Fubp3	G	A	2: 31,608,137	R101H	probably damaging	Het
Gba2	C	A	4: 43,569,927	G429C	probably damaging	Het
Gimap1	T	C	6: 48,741,429		probably benign	Het
Gm17657	A	T	17: 29,519,571	F74I	probably benign	Het
Gnas	A	G	2: 174,298,511	T158A	possibly damaging	Het
Gpc5	T	C	14: 115,552,328	V538A	probably damaging	Het
Greb1l	T	C	18: 10,458,781		probably benign	Het
H2-M10.5	G	A	17: 36,774,728	G260R	probably damaging	Het
Hbs1l	A	C	10: 21,349,323	Q412H	probably benign	Het
Hecw1	G	T	13: 14,236,941	T1058N	probably damaging	Het
Heph	A	T	X: 96,558,084	T1027S	probably damaging	Het
Hoga1	A	C	19: 42,046,075	E53A	possibly damaging	Het
Insrr	T	G	3: 87,814,437		probably benign	Het
Ipo11	A	T	13: 106,892,461	V328D	probably damaging	Het
Jakmip1	T	C	5: 37,118,873	V509A	probably damaging	Het
Jmjd1c	T	C	10: 67,225,789	V1307A	probably benign	Het
Kmt2a	T	A	9: 44,847,571	S1027C	probably damaging	Het
Kprp	G	C	3: 92,824,357	P462R	unknown	Het
Lman1l	T	C	9: 57,614,101	T193A	probably benign	Het
Lrit3	A	T	3: 129,791,296	V271D	probably damaging	Het
Map4	T	A	9: 109,979,103		probably benign	Het
Mark4	A	C	7: 19,448,673		probably benign	Het
Mfsd14b	A	G	13: 65,078,445	V142A	probably benign	Het
Mis18bp1	A	T	12: 65,161,453	I162N	possibly damaging	Het
Mrpl43	A	T	19: 45,005,952		probably benign	Het
Mrpl47	A	G	3: 32,736,693	F16S	probably benign	Het
Myh2	G	T	11: 67,178,967	G380C	probably damaging	Het
Myo15	T	C	11: 60,521,638	Y3284H	probably damaging	Het
Nectin2	A	G	7: 19,733,223		probably benign	Het
Neu3	A	G	7: 99,814,183	V111A	probably damaging	Het
Nol4l	T	C	2: 153,417,684		probably null	Het
Nphp3	C	A	9: 104,023,434	H510Q	probably damaging	Het
Nprl3	T	A	11: 32,233,118		probably null	Het
Olfr292	A	G	7: 86,695,308	N284S	probably damaging	Het
Olfr48	T	C	2: 89,844,443	T177A	probably benign	Het
Olfr598	T	C	7: 103,328,963	V159A	probably benign	Het
Olfr791	T	C	10: 129,526,896	I223T	possibly damaging	Het
Pex1	A	G	5: 3,606,130	E319G	possibly damaging	Het
Phtf1	C	A	3: 104,004,469	T709K	probably damaging	Het
Plek2	A	T	12: 78,892,172	L271Q	probably damaging	Het
Plekhg5	T	A	4: 152,107,469	C421*	probably null	Het
Polk	A	C	13: 96,484,179	C525W	probably damaging	Het
Ppfibp2	T	C	7: 107,729,174	S471P	probably damaging	Het
Prickle2	A	T	6: 92,458,565	F74L	probably benign	Het
Prl7d1	A	T	13: 27,712,055	V113D	probably benign	Het
Prr14	C	T	7: 127,472,095		probably benign	Het
Ptpro	T	A	6: 137,443,594	V1007D	probably damaging	Het
Ret	A	G	6: 118,178,610	V375A	probably damaging	Het
Rora	T	C	9: 69,361,746	F41S	probably damaging	Het
Sall1	T	C	8: 89,031,758	T573A	probably benign	Het
Shb	A	G	4: 45,458,321	V281A	possibly damaging	Het
Slc25a26	A	T	6: 94,592,410		probably null	Het
Sltm	T	C	9: 70,586,081	F769L	probably damaging	Het
Snx9	T	A	17: 5,918,413	M328K	probably damaging	Het
Stk25	G	T	1: 93,624,591	Q356K	probably benign	Het
Svep1	T	A	4: 58,128,858	Y613F	possibly damaging	Het
Syne4	G	A	7: 30,316,744	A195T	probably damaging	Het
Tmem8	T	C	17: 26,117,114	L130S	probably benign	Het
Trcg1	C	T	9: 57,242,333	T396M	probably damaging	Het
Trim30b	A	G	7: 104,357,298	V117A	possibly damaging	Het
Trpc4	T	C	3: 54,302,090		probably benign	Het
Ttll4	A	G	1: 74,688,280	H827R	probably damaging	Het
Urgcp	T	C	11: 5,717,477	E287G	probably damaging	Het
Usp2	G	T	9: 44,092,784	L319F	probably damaging	Het
Vmn1r167	A	T	7: 23,505,087	V168D	probably damaging	Het
Vmn2r100	C	A	17: 19,522,120	P252Q	possibly damaging	Het
Vmn2r61	A	T	7: 42,266,018	I130F	probably benign	Het
Vmn2r63	A	T	7: 42,928,528	Y195*	probably null	Het
Vmn2r81	T	C	10: 79,293,449	S725P	probably damaging	Het
Wnt10b	A	G	15: 98,772,937		probably benign	Het
Zcchc6	A	G	13: 59,800,317	V328A	probably benign	Het
Zfp292	T	C	4: 34,807,194	E1950G	probably damaging	Het
Zfyve16	A	G	13: 92,516,520		probably benign	Het

Other mutations in Asap1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00428:Asap1	APN	15	64119954	splice site	probably benign
IGL00473:Asap1	APN	15	64173215	splice site	probably benign
IGL00519:Asap1	APN	15	64110942	missense	probably damaging	1.00
IGL01304:Asap1	APN	15	64312449	missense	probably damaging	1.00
IGL01510:Asap1	APN	15	64158928	missense	probably damaging	1.00
IGL02208:Asap1	APN	15	64122033	missense	probably damaging	1.00
IGL02338:Asap1	APN	15	64123670	critical splice donor site	probably null
IGL02429:Asap1	APN	15	64167740	missense	probably damaging	1.00
IGL02565:Asap1	APN	15	64129165	splice site	probably benign
IGL02644:Asap1	APN	15	64111062	missense	probably damaging	1.00
IGL02684:Asap1	APN	15	64094169	missense	probably benign
IGL02707:Asap1	APN	15	64129274	missense	probably damaging	1.00
IGL03052:Asap1	APN	15	64153834	splice site	probably benign
IGL03153:Asap1	APN	15	64160274	missense	probably damaging	1.00
A4554:Asap1	UTSW	15	64124711	splice site	probably benign
PIT4378001:Asap1	UTSW	15	64135848	missense	probably damaging	0.99
R0081:Asap1	UTSW	15	64099564	missense	probably damaging	1.00
R1414:Asap1	UTSW	15	64158884	missense	possibly damaging	0.92
R1437:Asap1	UTSW	15	64120107	missense	probably damaging	0.96
R1474:Asap1	UTSW	15	64120020	missense	probably benign	0.01
R1489:Asap1	UTSW	15	64172730	missense	probably damaging	1.00
R1553:Asap1	UTSW	15	64152852	missense	probably benign	0.31
R1603:Asap1	UTSW	15	64129257	missense	probably damaging	1.00
R1636:Asap1	UTSW	15	64123912	missense	probably damaging	1.00
R1645:Asap1	UTSW	15	64089475	missense	probably damaging	0.99
R1861:Asap1	UTSW	15	64135798	splice site	probably benign
R2136:Asap1	UTSW	15	64110959	missense	probably damaging	1.00
R2351:Asap1	UTSW	15	64135804	critical splice donor site	probably null
R4436:Asap1	UTSW	15	64349843	missense	probably benign	0.03
R4618:Asap1	UTSW	15	64152895	missense	probably damaging	1.00
R4868:Asap1	UTSW	15	64094181	missense	probably benign
R5077:Asap1	UTSW	15	64127423	missense	probably damaging	1.00
R5333:Asap1	UTSW	15	64127414	missense	possibly damaging	0.79
R5391:Asap1	UTSW	15	64094052	missense	possibly damaging	0.57
R5493:Asap1	UTSW	15	64130151	missense	possibly damaging	0.85
R5665:Asap1	UTSW	15	64312453	missense	probably damaging	1.00
R5756:Asap1	UTSW	15	64167707	missense	probably damaging	1.00
R5790:Asap1	UTSW	15	64094265	missense	probably damaging	1.00
R6139:Asap1	UTSW	15	64166539	missense	possibly damaging	0.87
R6194:Asap1	UTSW	15	64129209	missense	probably damaging	1.00
R6361:Asap1	UTSW	15	64349823	splice site	probably null
R6751:Asap1	UTSW	15	64094412	missense	possibly damaging	0.86
R7143:Asap1	UTSW	15	64191528	missense	probably damaging	1.00
R7218:Asap1	UTSW	15	64130250	missense	probably damaging	1.00
R7225:Asap1	UTSW	15	64130250	missense	probably damaging	1.00
R7305:Asap1	UTSW	15	64130250	missense	probably damaging	1.00
R7310:Asap1	UTSW	15	64099530	critical splice donor site	probably null
R7439:Asap1	UTSW	15	64130256	missense	probably damaging	1.00
R7441:Asap1	UTSW	15	64130256	missense	probably damaging	1.00
R7488:Asap1	UTSW	15	64120125	missense	probably benign	0.29
R7597:Asap1	UTSW	15	64312455	missense	probably benign	0.37
R7708:Asap1	UTSW	15	64152872	missense	probably damaging	1.00
R7871:Asap1	UTSW	15	64092076	missense	probably damaging	1.00
R7990:Asap1	UTSW	15	64172737	splice site	probably null
R8163:Asap1	UTSW	15	64092050	missense	probably damaging	1.00
R8171:Asap1	UTSW	15	64110966	missense	probably damaging	1.00
R8416:Asap1	UTSW	15	64130223	missense	possibly damaging	0.83

Predicted Primers

PCR Primer
(F):5'- TGCTTCTGGATGGCATCTCTGGAC -3'
(R):5'- TCCCACAGTGGCACTAAGGAAGAC -3'

Sequencing Primer
(F):5'- GACTGTACATTTGGAGACAGATCCC -3'
(R):5'- GACGGAGACCAGCCATC -3'

Posted On

2013-06-11