Mutagenetix > Incidental Mutation

Incidental Mutation 'R5870:Or7e176'

455132

Institutional Source

Beutler Lab

Gene Symbol

Or7e176

Ensembl Gene

ENSMUSG00000066897

Gene Name

olfactory receptor family 7 subfamily E member 176

Synonyms

Olfr872, GA_x6K02T2PVTD-13999915-14000844, MOR145-3

MMRRC Submission

044078-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.050) question?

Stock #

R5870 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

20148458-20172209 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 20171874 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Aspartic acid to Glycine at position 246 (D246G)

Ref Sequence

ENSEMBL: ENSMUSP00000083665 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000086474]

AlphaFold

E9PVW2

Predicted Effect

probably benign
Transcript: ENSMUST00000086474
AA Change: D246G

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000083665
Gene: ENSMUSG00000066897
AA Change: D246G

Domain	Start	End	E-Value	Type
Pfam:7tm_4	45	321	3.8e-45	PFAM
Pfam:7TM_GPCR_Srsx	49	310	1.4e-9	PFAM
Pfam:7tm_1	55	304	4.2e-21	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212245

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000212574

Meta Mutation Damage Score

0.0845

Coding Region Coverage

1x: 99.9%
3x: 99.4%
10x: 97.2%
20x: 91.1%

Validation Efficiency

93% (84/90)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 71 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adcy9	A	G	16: 4,236,232 (GRCm39)	V156A	probably damaging	Het
Ak6	C	A	13: 100,791,932 (GRCm39)	P125Q	probably damaging	Het
Aqp4	A	C	18: 15,532,946 (GRCm39)	V49G	probably damaging	Het
Arfgef1	A	G	1: 10,251,163 (GRCm39)	I874T	probably damaging	Het
Arid1a	T	C	4: 133,408,387 (GRCm39)	D2040G	unknown	Het
Atp1a3	T	G	7: 24,697,003 (GRCm39)	D220A	probably benign	Het
C2cd4c	A	T	10: 79,448,043 (GRCm39)	I368N	possibly damaging	Het
Ccnt1	G	A	15: 98,441,394 (GRCm39)	Q625*	probably null	Het
Cd177	T	A	7: 24,455,757 (GRCm39)	H255L	probably benign	Het
Cdipt	G	A	7: 126,578,094 (GRCm39)	V114M	probably benign	Het
Coro1b	T	A	19: 4,199,384 (GRCm39)	H14Q	probably damaging	Het
Ctdp1	T	A	18: 80,451,901 (GRCm39)	D158V	unknown	Het
Cts7	A	T	13: 61,503,545 (GRCm39)	S140T	probably damaging	Het
Dlgap3	T	A	4: 127,089,502 (GRCm39)	L366*	probably null	Het
Dnah9	C	T	11: 65,976,036 (GRCm39)	A1338T	probably benign	Het
Dock7	T	C	4: 98,952,199 (GRCm39)	I424V	probably benign	Het
Dock8	G	T	19: 25,109,490 (GRCm39)	A891S	probably benign	Het
Dync2i1	A	G	12: 116,219,865 (GRCm39)	S26P	possibly damaging	Het
Elmod3	A	G	6: 72,571,721 (GRCm39)		probably null	Het
Eps15	G	A	4: 109,218,507 (GRCm39)	E107K	probably damaging	Het
Esco1	A	T	18: 10,593,744 (GRCm39)		probably null	Het
Fuz	A	G	7: 44,549,742 (GRCm39)	T407A	probably damaging	Het
Galr1	A	T	18: 82,424,197 (GRCm39)	F27I	probably benign	Het
Glt1d1	A	G	5: 127,754,344 (GRCm39)	Y182C	probably damaging	Het
Gm37240	A	T	3: 84,597,828 (GRCm39)		probably benign	Het
Gm37610	A	G	6: 41,061,848 (GRCm39)		noncoding transcript	Het
Gm6658	G	T	8: 91,635,020 (GRCm39)		probably benign	Het
Gm9376	A	G	14: 118,504,789 (GRCm39)	T74A	possibly damaging	Het
Hadha	G	A	5: 30,349,284 (GRCm39)	S109F	possibly damaging	Het
Herc3	A	T	6: 58,893,435 (GRCm39)	Q899L	probably benign	Het
Ift172	C	T	5: 31,434,284 (GRCm39)	E485K	probably benign	Het
Lrrc8e	A	G	8: 4,285,725 (GRCm39)	K650R	possibly damaging	Het
Ly6d	A	T	15: 74,635,381 (GRCm39)	V10D	possibly damaging	Het
Med27	A	G	2: 29,279,823 (GRCm39)		probably null	Het
Med29	A	T	7: 28,091,922 (GRCm39)	V56E	probably damaging	Het
Mobp	A	G	9: 119,996,919 (GRCm39)	K17E	probably damaging	Het
Mrpl37	G	A	4: 106,923,919 (GRCm39)	T25I	probably benign	Het
Myh1	A	G	11: 67,092,805 (GRCm39)	D33G	possibly damaging	Het
Nrg3	T	A	14: 39,194,586 (GRCm39)	I58F	possibly damaging	Het
Or52e2	A	G	7: 102,804,948 (GRCm39)	I2T	probably benign	Het
Padi1	T	A	4: 140,553,892 (GRCm39)	D359V	probably benign	Het
Pcdh7	T	C	5: 57,877,753 (GRCm39)	V436A	possibly damaging	Het
Pgm3	C	A	9: 86,452,414 (GRCm39)	K15N	probably damaging	Het
Phip	A	T	9: 82,790,730 (GRCm39)		probably benign	Het
Pot1a	G	A	6: 25,778,950 (GRCm39)	T48I	possibly damaging	Het
Ppic	T	C	18: 53,542,333 (GRCm39)	K125R	probably benign	Het
Ppm1j	T	C	3: 104,692,811 (GRCm39)	V440A	possibly damaging	Het
Prg4	T	A	1: 150,331,300 (GRCm39)	K458*	probably null	Het
Rd3	A	T	1: 191,717,261 (GRCm39)	M244L	probably benign	Het
Rflnb	A	G	11: 75,912,864 (GRCm39)	Y175H	probably benign	Het
Rnf157	T	A	11: 116,237,900 (GRCm39)	S574C	probably benign	Het
Sardh	A	G	2: 27,110,653 (GRCm39)		probably null	Het
Senp3	C	T	11: 69,569,048 (GRCm39)		probably null	Het
Siglec1	G	A	2: 130,914,767 (GRCm39)	R1450C	probably damaging	Het
Sim2	A	G	16: 93,924,193 (GRCm39)	H446R	probably damaging	Het
Spon1	T	C	7: 113,631,021 (GRCm39)	I444T	probably damaging	Het
Srebf1	T	A	11: 60,094,410 (GRCm39)	Q568H	possibly damaging	Het
Stxbp4	A	T	11: 90,428,782 (GRCm39)	I441N	possibly damaging	Het
Sugt1	G	A	14: 79,846,451 (GRCm39)	V163I	probably benign	Het
Surf1	G	T	2: 26,806,271 (GRCm39)		probably benign	Het
Synj2	A	G	17: 6,088,128 (GRCm39)	E1348G	probably benign	Het
Tc2n	A	T	12: 101,619,111 (GRCm39)	V349D	probably damaging	Het
Ten1	A	G	11: 116,105,751 (GRCm39)	R112G	possibly damaging	Het
Tm9sf4	A	G	2: 153,036,201 (GRCm39)	D321G	probably damaging	Het
Ttll12	A	T	15: 83,461,237 (GRCm39)	M594K	probably damaging	Het
Ttn	T	A	2: 76,703,058 (GRCm39)		probably benign	Het
Usp28	C	T	9: 48,937,285 (GRCm39)	Q185*	probably null	Het
Vmn2r112	A	G	17: 22,838,004 (GRCm39)	I822V	probably benign	Het
Zc3hc1	A	T	6: 30,382,682 (GRCm39)	L88*	probably null	Het
Zfr	T	A	15: 12,160,701 (GRCm39)	V758D	probably damaging	Het
Zfyve27	T	G	19: 42,160,110 (GRCm39)	L42R	probably benign	Het

Other mutations in Or7e176

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01904:Or7e176	APN	9	20,171,586 (GRCm39)	missense	probably damaging	0.99
IGL02048:Or7e176	APN	9	20,171,784 (GRCm39)	missense	possibly damaging	0.79
IGL02232:Or7e176	APN	9	20,171,511 (GRCm39)	missense	probably damaging	1.00
IGL02314:Or7e176	APN	9	20,171,774 (GRCm39)	missense	probably benign	0.03
IGL03290:Or7e176	APN	9	20,171,556 (GRCm39)	missense	probably damaging	1.00
R0410:Or7e176	UTSW	9	20,171,797 (GRCm39)	missense	probably benign	0.03
R1482:Or7e176	UTSW	9	20,172,020 (GRCm39)	missense	possibly damaging	0.89
R1521:Or7e176	UTSW	9	20,171,728 (GRCm39)	missense	possibly damaging	0.91
R4930:Or7e176	UTSW	9	20,171,313 (GRCm39)	missense	probably damaging	1.00
R5457:Or7e176	UTSW	9	20,171,574 (GRCm39)	missense	probably damaging	1.00
R6141:Or7e176	UTSW	9	20,171,754 (GRCm39)	missense	probably benign	0.00
R7283:Or7e176	UTSW	9	20,171,555 (GRCm39)	missense	probably damaging	0.98
R8691:Or7e176	UTSW	9	20,171,747 (GRCm39)	missense	probably benign	0.36
R8882:Or7e176	UTSW	9	20,171,256 (GRCm39)	missense	probably benign	0.06
R9556:Or7e176	UTSW	9	20,171,651 (GRCm39)	missense	probably benign	0.00
R9618:Or7e176	UTSW	9	20,171,639 (GRCm39)	missense	possibly damaging	0.80
R9752:Or7e176	UTSW	9	20,171,204 (GRCm39)	missense	probably benign	0.10
X0025:Or7e176	UTSW	9	20,171,782 (GRCm39)	missense	probably benign	0.01

Predicted Primers

PCR Primer
(F):5'- ACTCTCAGGTGCACAATTTGATTG -3'
(R):5'- CTCAGGCTGTAGATGAAGGG -3'

Sequencing Primer
(F):5'- TCAATGACATGGAAATCTCTAACTTC -3'
(R):5'- GATTAAGCATAGGTGTGACCACTGTG -3'

Posted On

2017-02-10