Mutagenetix > Incidental Mutation

Incidental Mutation 'R5876:Olfr8'

455543

Institutional Source

Beutler Lab

Gene Symbol

Olfr8

Ensembl Gene

ENSMUSG00000094080

Gene Name

olfactory receptor 8

Synonyms

MOR139-5P, GA_x6K02T2QGN0-2857086-2856154

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.136) question?

Stock #

R5876 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

78950636-78958378 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

A to T at 78955357 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Phenylalanine at position 51 (I51F)

Ref Sequence

ENSEMBL: ENSMUSP00000148856 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000081571] [ENSMUST00000203851] [ENSMUST00000214952]

AlphaFold

Q60892

Predicted Effect

probably benign
Transcript: ENSMUST00000081571
AA Change: I51F

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000080282
Gene: ENSMUSG00000094080
AA Change: I51F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	309	1.3e-47	PFAM
Pfam:7tm_1	42	291	3e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000203851
AA Change: I51F

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

SMART Domains

Protein: ENSMUSP00000144916
Gene: ENSMUSG00000094080
AA Change: I51F

Domain	Start	End	E-Value	Type
Pfam:7tm_4	32	309	1.3e-47	PFAM
Pfam:7tm_1	42	291	3e-20	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000214952
AA Change: I51F

PolyPhen 2 Score 0.147 (Sensitivity: 0.92; Specificity: 0.87)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000216819

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.6%

Validation Efficiency

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1700034E13Rik	A	G	18: 52,663,582 (GRCm38)	D64G	possibly damaging	Het
Ano2	T	A	6: 126,039,279 (GRCm38)	M925K	possibly damaging	Het
Arnt2	T	C	7: 84,347,512 (GRCm38)	T69A	probably damaging	Het
Asap2	T	A	12: 21,212,809 (GRCm38)	N229K	possibly damaging	Het
Atp13a3	T	A	16: 30,362,734 (GRCm38)	N23Y	probably benign	Het
Cbr4	G	A	8: 61,490,593 (GRCm38)	G91R	possibly damaging	Het
Cdc27	A	T	11: 104,515,418 (GRCm38)	C624S	probably benign	Het
Cdc42bpg	T	A	19: 6,310,815 (GRCm38)	I201N	probably damaging	Het
Cdh5	A	G	8: 104,142,577 (GRCm38)	Y645C	probably damaging	Het
Celsr2	C	T	3: 108,413,943 (GRCm38)	V518I	probably damaging	Het
Clca4b	T	C	3: 144,912,060 (GRCm38)	T761A	possibly damaging	Het
Cpne4	C	A	9: 104,925,770 (GRCm38)	S204R	probably damaging	Het
Dcaf1	T	C	9: 106,863,650 (GRCm38)	W1279R	probably damaging	Het
Dennd4a	C	T	9: 64,911,755 (GRCm38)	P1731S	probably damaging	Het
Dmxl1	T	A	18: 49,870,984 (GRCm38)	V892D	possibly damaging	Het
Fam131b	A	G	6: 42,321,248 (GRCm38)		probably null	Het
Fam83b	T	A	9: 76,491,850 (GRCm38)	D657V	possibly damaging	Het
Fam83e	T	A	7: 45,722,363 (GRCm38)		probably null	Het
Fbxo4	A	T	15: 3,977,819 (GRCm38)	I121N	probably damaging	Het
Gabrg2	A	G	11: 41,968,820 (GRCm38)	S202P	probably damaging	Het
Gm10471	T	C	5: 26,084,718 (GRCm38)	E237G	probably damaging	Het
Grid2	T	A	6: 64,663,162 (GRCm38)	I788N	probably damaging	Het
Grik4	T	A	9: 42,688,023 (GRCm38)	N53Y	probably damaging	Het
Hipk2	A	C	6: 38,730,867 (GRCm38)		probably null	Het
Hps5	T	C	7: 46,789,196 (GRCm38)	T38A	probably damaging	Het
Hs1bp3	A	G	12: 8,341,843 (GRCm38)	D315G	possibly damaging	Het
Kdm4a	C	T	4: 118,138,876 (GRCm38)	M985I	probably damaging	Het
Kdm5d	A	G	Y: 900,525 (GRCm38)	Y190C	probably damaging	Het
Krt86	T	C	15: 101,476,610 (GRCm38)	S295P	probably damaging	Het
Matr3	T	A	18: 35,587,738 (GRCm38)	D413E	probably benign	Het
Mbd5	T	A	2: 49,274,645 (GRCm38)	F319L	probably damaging	Het
Mcoln1	T	A	8: 3,510,910 (GRCm38)	Y411N	probably damaging	Het
Mpdz	C	A	4: 81,285,474 (GRCm38)	E1863*	probably null	Het
Mrps9	A	G	1: 42,895,378 (GRCm38)	E173G	probably damaging	Het
Osmr	T	C	15: 6,821,047 (GRCm38)	T692A	probably benign	Het
Pkhd1l1	A	T	15: 44,578,588 (GRCm38)	H3641L	possibly damaging	Het
Pml	T	C	9: 58,233,182 (GRCm38)	T421A	possibly damaging	Het
Podxl	A	G	6: 31,528,456 (GRCm38)		probably null	Het
Ppargc1b	C	G	18: 61,309,093 (GRCm38)	D591H	probably damaging	Het
Prkag3	A	G	1: 74,748,816 (GRCm38)		probably benign	Het
Proz	A	G	8: 13,073,448 (GRCm38)	R240G	probably benign	Het
Ptpn13	C	A	5: 103,476,960 (GRCm38)	D43E	probably damaging	Het
Rbm5	T	G	9: 107,760,326 (GRCm38)	K135N	probably damaging	Het
Ska1	G	A	18: 74,197,528 (GRCm38)	T201M	probably damaging	Het
Slc35f5	A	C	1: 125,587,363 (GRCm38)		probably null	Het
Slc9a3	T	C	13: 74,161,723 (GRCm38)	L510P	probably damaging	Het
Svil	A	G	18: 5,082,828 (GRCm38)	K1231E	probably damaging	Het
Tanc2	T	C	11: 105,922,613 (GRCm38)	S1628P	possibly damaging	Het
Tm9sf3	T	A	19: 41,240,584 (GRCm38)	D260V	probably damaging	Het
Ttc9	G	T	12: 81,631,622 (GRCm38)	R73L	probably damaging	Het
Vps13b	A	G	15: 35,917,061 (GRCm38)	I3684V	probably damaging	Het
Vps8	T	C	16: 21,461,439 (GRCm38)		probably null	Het
Vwa3b	T	A	1: 37,076,439 (GRCm38)	I328N	probably damaging	Het
Wdr1	C	T	5: 38,530,023 (GRCm38)	V222M	probably benign	Het
Xpnpep1	T	A	19: 52,997,008 (GRCm38)	T530S	probably damaging	Het
Zc3h6	C	T	2: 128,993,277 (GRCm38)	S111F	probably benign	Het
Zfp768	G	A	7: 127,344,546 (GRCm38)	P137S	probably benign	Het

Other mutations in Olfr8

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01022:Olfr8	APN	10	78,955,354 (GRCm38)	missense	possibly damaging	0.48
IGL01480:Olfr8	APN	10	78,956,144 (GRCm38)	utr 3 prime	probably benign
IGL02505:Olfr8	APN	10	78,955,933 (GRCm38)	missense	probably benign	0.02
IGL02543:Olfr8	APN	10	78,955,939 (GRCm38)	missense	probably damaging	1.00
IGL03323:Olfr8	APN	10	78,955,600 (GRCm38)	missense	probably benign
PIT4466001:Olfr8	UTSW	10	78,955,842 (GRCm38)	missense	probably benign	0.00
R1496:Olfr8	UTSW	10	78,955,848 (GRCm38)	missense	probably benign	0.41
R1754:Olfr8	UTSW	10	78,955,697 (GRCm38)	missense	probably damaging	0.99
R1878:Olfr8	UTSW	10	78,955,805 (GRCm38)	missense	possibly damaging	0.62
R2760:Olfr8	UTSW	10	78,956,042 (GRCm38)	missense	probably damaging	0.99
R4202:Olfr8	UTSW	10	78,955,295 (GRCm38)	missense	probably benign
R4206:Olfr8	UTSW	10	78,955,283 (GRCm38)	missense	probably benign	0.00
R4517:Olfr8	UTSW	10	78,956,043 (GRCm38)	nonsense	probably null
R4613:Olfr8	UTSW	10	78,956,065 (GRCm38)	missense	probably damaging	1.00
R4799:Olfr8	UTSW	10	78,956,097 (GRCm38)	missense	probably null	0.92
R4979:Olfr8	UTSW	10	78,955,932 (GRCm38)	nonsense	probably null
R5008:Olfr8	UTSW	10	78,956,071 (GRCm38)	missense	probably damaging	1.00
R5700:Olfr8	UTSW	10	78,955,484 (GRCm38)	missense	probably damaging	1.00
R6439:Olfr8	UTSW	10	78,955,984 (GRCm38)	missense	probably damaging	1.00
R6930:Olfr8	UTSW	10	78,955,781 (GRCm38)	missense	possibly damaging	0.84
R7110:Olfr8	UTSW	10	78,955,450 (GRCm38)	missense	possibly damaging	0.83
R7405:Olfr8	UTSW	10	78,955,697 (GRCm38)	missense	probably benign	0.14
R7524:Olfr8	UTSW	10	78,955,491 (GRCm38)	nonsense	probably null
R8198:Olfr8	UTSW	10	78,955,724 (GRCm38)	missense	probably damaging	0.97
R9227:Olfr8	UTSW	10	78,956,095 (GRCm38)	missense	possibly damaging	0.92
R9230:Olfr8	UTSW	10	78,956,095 (GRCm38)	missense	possibly damaging	0.92
Z1176:Olfr8	UTSW	10	78,955,219 (GRCm38)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TCTCATAGACACATGTGACTGATG -3'
(R):5'- TAGCCACATATCGGTCATAGGCC -3'

Sequencing Primer
(F):5'- CATAGACACATGTGACTGATGGAATC -3'
(R):5'- CCAGGAGAAAGTTGTCCAATTCTGC -3'

Posted On

2017-02-10