Mutagenetix > Incidental Mutation

Incidental Mutation 'R5877:Wdfy3'

455591

Institutional Source

Beutler Lab

Gene Symbol

Wdfy3

Ensembl Gene

ENSMUSG00000043940

Gene Name

WD repeat and FYVE domain containing 3

Synonyms

D5Ertd66e, Bwf1, Bchs, 2610509D04Rik, Ggtb3

MMRRC Submission

044083-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.951) question?

Stock #

R5877 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

101832956-102069921 bp(-) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 101869989 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Valine at position 2562 (I2562V)

Ref Sequence

ENSEMBL: ENSMUSP00000148521 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000053177] [ENSMUST00000174598] [ENSMUST00000212024]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000053177
AA Change: I2558V

PolyPhen 2 Score 0.991 (Sensitivity: 0.71; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000052607
Gene: ENSMUSG00000043940
AA Change: I2558V

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
low complexity region	1408	1417	N/A	INTRINSIC
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2517	2638	3.1e-17	PFAM
Beach	2677	2958	2.54e-217	SMART
WD40	3054	3088	1.28e1	SMART
WD40	3098	3137	7.73e-6	SMART
WD40	3140	3178	8.29e-1	SMART
WD40	3183	3227	3.09e-1	SMART
low complexity region	3253	3274	N/A	INTRINSIC
low complexity region	3307	3318	N/A	INTRINSIC
WD40	3381	3420	1.33e1	SMART
FYVE	3428	3497	3.18e-27	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000174598
AA Change: I2576V

PolyPhen 2 Score 0.989 (Sensitivity: 0.72; Specificity: 0.97)

SMART Domains

Protein: ENSMUSP00000134244
Gene: ENSMUSG00000043940
AA Change: I2576V

Domain	Start	End	E-Value	Type
low complexity region	463	481	N/A	INTRINSIC
Pfam:DUF4704	1392	1597	6.6e-11	PFAM
low complexity region	1629	1644	N/A	INTRINSIC
Pfam:PH_BEACH	2588	2656	1.8e-14	PFAM
Beach	2695	2976	2.54e-217	SMART
WD40	3072	3106	1.28e1	SMART
WD40	3116	3155	7.73e-6	SMART
WD40	3158	3196	8.29e-1	SMART
WD40	3201	3245	3.09e-1	SMART
low complexity region	3271	3292	N/A	INTRINSIC
low complexity region	3325	3336	N/A	INTRINSIC
WD40	3399	3438	1.33e1	SMART
FYVE	3446	3515	3.18e-27	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000212024
AA Change: I2562V

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

Meta Mutation Damage Score

0.1604

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.7%

Validation Efficiency

97% (68/70)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a phosphatidylinositol 3-phosphate-binding protein that functions as a master conductor for aggregate clearance by autophagy. This protein shuttles from the nuclear membrane to colocalize with aggregated proteins, where it complexes with other autophagic components to achieve macroautophagy-mediated clearance of these aggregated proteins. However, it is not necessary for starvation-induced macroautophagy. [provided by RefSeq, May 2010]
PHENOTYPE: Mice homozygous for hypomorphic mutations of this gene exhibit perinatal lethality, altered neural progenitor divisions and neuronal migration, a regionally enlarged cerebral cortex, and focal cortical dysplasias. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 63 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
8030462N17Rik	G	T	18: 77,643,206 (GRCm38)		probably null	Het
Adam6b	A	T	12: 113,490,202 (GRCm38)	H213L	probably damaging	Het
Alk	A	G	17: 71,967,526 (GRCm38)	W597R	probably damaging	Het
Ash1l	G	A	3: 88,981,584 (GRCm38)	V257I	probably benign	Het
Atp9b	C	T	18: 80,752,789 (GRCm38)	V957I	probably benign	Het
Birc3	A	C	9: 7,849,346 (GRCm38)	C576G	probably damaging	Het
Brca2	T	C	5: 150,543,221 (GRCm38)	M2150T	possibly damaging	Het
C3ar1	T	G	6: 122,850,622 (GRCm38)	D212A	probably benign	Het
Cacna1s	T	C	1: 136,100,667 (GRCm38)	F914S	probably damaging	Het
Camk1d	T	C	2: 5,565,665 (GRCm38)	I62V	probably benign	Het
Caskin1	T	C	17: 24,505,265 (GRCm38)	M1009T	possibly damaging	Het
Casp2	A	G	6: 42,276,637 (GRCm38)		probably benign	Het
Cd2bp2	T	A	7: 127,194,799 (GRCm38)	Y106F	probably damaging	Het
Celsr3	G	A	9: 108,845,727 (GRCm38)	C2763Y	probably damaging	Het
Cyp2g1	T	G	7: 26,816,640 (GRCm38)	L310V	possibly damaging	Het
Dusp22	G	A	13: 30,707,961 (GRCm38)	V99M	probably damaging	Het
Fam131b	G	A	6: 42,320,979 (GRCm38)	S92L	probably benign	Het
Fbxw10	A	T	11: 62,857,716 (GRCm38)	N390Y	probably damaging	Het
Flg2	A	T	3: 93,203,449 (GRCm38)	H928L	unknown	Het
Gapdhs	T	C	7: 30,732,347 (GRCm38)	T280A	probably damaging	Het
Hdac11	A	G	6: 91,157,542 (GRCm38)	K50E	probably damaging	Het
Hif3a	G	T	7: 17,051,146 (GRCm38)	A181E	probably damaging	Het
Ikbkap	A	T	4: 56,787,807 (GRCm38)	W375R	probably damaging	Het
Itgb8	A	G	12: 119,202,536 (GRCm38)	S87P	probably benign	Het
Klhl12	T	A	1: 134,483,820 (GRCm38)	Y302*	probably null	Het
Lhx1	C	A	11: 84,522,239 (GRCm38)	D30Y	probably damaging	Het
Mrpl40	A	G	16: 18,872,385 (GRCm38)	Y148H	probably damaging	Het
Myh13	A	T	11: 67,353,658 (GRCm38)	H1007L	possibly damaging	Het
Nhlrc2	T	C	19: 56,570,584 (GRCm38)	I167T	probably damaging	Het
Nxpe3	A	T	16: 55,866,201 (GRCm38)	I148K	probably damaging	Het
Odf2l	A	G	3: 145,129,010 (GRCm38)		probably null	Het
Otud7b	C	T	3: 96,151,960 (GRCm38)	R347*	probably null	Het
P3h4	G	T	11: 100,414,017 (GRCm38)	H181Q	probably benign	Het
P4htm	T	A	9: 108,583,733 (GRCm38)	Y180F	possibly damaging	Het
Pcna-ps2	A	G	19: 9,284,099 (GRCm38)	I241V	probably benign	Het
Pcnx2	T	C	8: 125,753,728 (GRCm38)	S1947G	probably damaging	Het
Piezo2	T	C	18: 63,113,934 (GRCm38)	I336V	probably benign	Het
Plcd1	T	C	9: 119,076,172 (GRCm38)	D157G	probably damaging	Het
Plekhm2	A	C	4: 141,639,693 (GRCm38)	I212S	probably damaging	Het
Pmvk	G	T	3: 89,464,369 (GRCm38)	C57F	probably benign	Het
Pole	T	C	5: 110,332,463 (GRCm38)	S91P	probably benign	Het
Ppargc1b	C	G	18: 61,309,093 (GRCm38)	D591H	probably damaging	Het
Ppp4r3b	A	T	11: 29,209,356 (GRCm38)	D570V	probably damaging	Het
Pxdn	T	A	12: 30,003,046 (GRCm38)	I1074N	probably damaging	Het
Ralgapa2	A	T	2: 146,388,569 (GRCm38)	D1025E	probably damaging	Het
Ralgps1	A	G	2: 33,243,628 (GRCm38)		probably benign	Het
Rasal1	C	T	5: 120,679,070 (GRCm38)		probably benign	Het
Rps2	A	T	17: 24,720,916 (GRCm38)		probably benign	Het
Scn7a	G	C	2: 66,699,873 (GRCm38)	Y709*	probably null	Het
Skint1	T	A	4: 112,021,523 (GRCm38)	C217*	probably null	Het
Slc50a1	T	C	3: 89,269,153 (GRCm38)	Y82C	probably damaging	Het
Slc9a4	T	C	1: 40,612,263 (GRCm38)	V567A	probably benign	Het
Snx27	A	T	3: 94,502,963 (GRCm38)	W469R	probably damaging	Het
Sos2	A	G	12: 69,596,795 (GRCm38)	L937P	probably damaging	Het
Ssh1	T	C	5: 113,943,120 (GRCm38)	T728A	probably benign	Het
Tnfrsf8	G	A	4: 145,292,687 (GRCm38)	R193C	probably benign	Het
Usp33	G	A	3: 152,379,596 (GRCm38)	C623Y	probably damaging	Het
Usp33	T	A	3: 152,379,476 (GRCm38)	M583K	possibly damaging	Het
Wnk2	T	C	13: 49,067,306 (GRCm38)	D1175G	probably damaging	Het
Zcchc11	T	C	4: 108,512,923 (GRCm38)	V673A	probably damaging	Het
Zfp341	G	A	2: 154,632,289 (GRCm38)	E338K	probably damaging	Het
Zfp398	A	G	6: 47,840,704 (GRCm38)		probably benign	Het
Zp2	T	A	7: 120,133,339 (GRCm38)	K661N	probably null	Het

Other mutations in Wdfy3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00332:Wdfy3	APN	5	101,915,338 (GRCm38)	critical splice donor site	probably null
IGL00567:Wdfy3	APN	5	101,912,030 (GRCm38)	splice site	probably benign
IGL01288:Wdfy3	APN	5	101,901,991 (GRCm38)	splice site	probably null
IGL01323:Wdfy3	APN	5	101,895,064 (GRCm38)	missense	probably damaging	1.00
IGL01352:Wdfy3	APN	5	101,944,120 (GRCm38)	missense	probably damaging	1.00
IGL01553:Wdfy3	APN	5	101,900,031 (GRCm38)	missense	probably benign
IGL01560:Wdfy3	APN	5	101,957,486 (GRCm38)	nonsense	probably null
IGL01566:Wdfy3	APN	5	101,896,588 (GRCm38)	splice site	probably benign
IGL01616:Wdfy3	APN	5	101,913,260 (GRCm38)	missense	probably damaging	0.97
IGL01630:Wdfy3	APN	5	101,907,488 (GRCm38)	missense	probably benign
IGL01791:Wdfy3	APN	5	101,937,412 (GRCm38)	missense	probably damaging	1.00
IGL01820:Wdfy3	APN	5	101,924,081 (GRCm38)	missense	probably benign	0.11
IGL01953:Wdfy3	APN	5	101,895,028 (GRCm38)	nonsense	probably null
IGL02121:Wdfy3	APN	5	101,898,510 (GRCm38)	missense	possibly damaging	0.85
IGL02167:Wdfy3	APN	5	101,961,157 (GRCm38)	missense	probably damaging	0.98
IGL02321:Wdfy3	APN	5	101,922,609 (GRCm38)	missense	probably damaging	0.99
IGL02327:Wdfy3	APN	5	101,888,192 (GRCm38)	missense	probably damaging	1.00
IGL02651:Wdfy3	APN	5	101,896,475 (GRCm38)	missense	probably benign	0.37
IGL02801:Wdfy3	APN	5	101,907,587 (GRCm38)	missense	probably damaging	1.00
IGL02839:Wdfy3	APN	5	101,968,920 (GRCm38)	missense	probably damaging	1.00
IGL02870:Wdfy3	APN	5	101,855,471 (GRCm38)	missense	probably damaging	1.00
IGL02997:Wdfy3	APN	5	101,894,912 (GRCm38)	missense	probably null	1.00
IGL03064:Wdfy3	APN	5	101,935,997 (GRCm38)	missense	probably damaging	0.99
IGL03090:Wdfy3	APN	5	101,866,276 (GRCm38)	missense	probably damaging	1.00
IGL03211:Wdfy3	APN	5	101,844,912 (GRCm38)	splice site	probably benign
IGL03237:Wdfy3	APN	5	101,844,599 (GRCm38)	missense	probably damaging	1.00
IGL03264:Wdfy3	APN	5	101,900,150 (GRCm38)	missense	probably damaging	1.00
Esurient	UTSW	5	101,944,103 (GRCm38)	missense	probably damaging	1.00
IGL02988:Wdfy3	UTSW	5	101,929,981 (GRCm38)	missense	probably damaging	0.99
PIT4382001:Wdfy3	UTSW	5	101,882,961 (GRCm38)	frame shift	probably null
R0010:Wdfy3	UTSW	5	101,848,349 (GRCm38)	missense	probably damaging	1.00
R0010:Wdfy3	UTSW	5	101,848,349 (GRCm38)	missense	probably damaging	1.00
R0025:Wdfy3	UTSW	5	101,845,046 (GRCm38)	missense	probably damaging	0.98
R0031:Wdfy3	UTSW	5	101,889,295 (GRCm38)	missense	probably damaging	0.97
R0047:Wdfy3	UTSW	5	101,944,033 (GRCm38)	missense	probably damaging	1.00
R0047:Wdfy3	UTSW	5	101,944,033 (GRCm38)	missense	probably damaging	1.00
R0053:Wdfy3	UTSW	5	101,844,614 (GRCm38)	missense	probably damaging	0.97
R0078:Wdfy3	UTSW	5	101,888,105 (GRCm38)	missense	possibly damaging	0.57
R0147:Wdfy3	UTSW	5	101,917,411 (GRCm38)	missense	probably benign	0.05
R0148:Wdfy3	UTSW	5	101,917,411 (GRCm38)	missense	probably benign	0.05
R0279:Wdfy3	UTSW	5	101,868,092 (GRCm38)	missense	probably damaging	1.00
R0380:Wdfy3	UTSW	5	101,948,966 (GRCm38)	missense	probably damaging	0.99
R0472:Wdfy3	UTSW	5	101,957,443 (GRCm38)	missense	probably benign	0.13
R0513:Wdfy3	UTSW	5	101,890,789 (GRCm38)	missense	probably damaging	0.96
R0594:Wdfy3	UTSW	5	101,906,185 (GRCm38)	missense	possibly damaging	0.94
R0601:Wdfy3	UTSW	5	101,836,172 (GRCm38)	missense	probably benign
R0787:Wdfy3	UTSW	5	101,957,388 (GRCm38)	missense	probably damaging	1.00
R0825:Wdfy3	UTSW	5	101,870,051 (GRCm38)	missense	probably damaging	1.00
R1122:Wdfy3	UTSW	5	101,882,966 (GRCm38)	missense	possibly damaging	0.94
R1167:Wdfy3	UTSW	5	101,875,931 (GRCm38)	missense	probably benign
R1350:Wdfy3	UTSW	5	101,898,552 (GRCm38)	missense	probably damaging	1.00
R1422:Wdfy3	UTSW	5	101,884,214 (GRCm38)	splice site	probably benign
R1446:Wdfy3	UTSW	5	101,851,310 (GRCm38)	missense	possibly damaging	0.68
R1452:Wdfy3	UTSW	5	101,937,738 (GRCm38)	missense	possibly damaging	0.91
R1457:Wdfy3	UTSW	5	101,917,579 (GRCm38)	missense	possibly damaging	0.57
R1543:Wdfy3	UTSW	5	101,844,081 (GRCm38)	missense	probably benign
R1633:Wdfy3	UTSW	5	101,981,548 (GRCm38)	missense	probably damaging	1.00
R1643:Wdfy3	UTSW	5	101,875,915 (GRCm38)	missense	possibly damaging	0.62
R1656:Wdfy3	UTSW	5	101,941,447 (GRCm38)	missense	probably damaging	1.00
R1720:Wdfy3	UTSW	5	101,926,525 (GRCm38)	frame shift	probably null
R1743:Wdfy3	UTSW	5	101,844,065 (GRCm38)	missense	probably benign	0.12
R1745:Wdfy3	UTSW	5	101,948,929 (GRCm38)	missense	probably damaging	0.96
R1850:Wdfy3	UTSW	5	101,894,999 (GRCm38)	missense	probably damaging	1.00
R1852:Wdfy3	UTSW	5	101,915,376 (GRCm38)	missense	probably benign	0.00
R1854:Wdfy3	UTSW	5	101,888,186 (GRCm38)	missense	probably benign	0.05
R1880:Wdfy3	UTSW	5	101,917,435 (GRCm38)	missense	probably benign	0.05
R1930:Wdfy3	UTSW	5	101,941,492 (GRCm38)	missense	probably damaging	1.00
R1931:Wdfy3	UTSW	5	101,941,492 (GRCm38)	missense	probably damaging	1.00
R1956:Wdfy3	UTSW	5	101,919,409 (GRCm38)	missense	probably benign	0.30
R1965:Wdfy3	UTSW	5	101,951,312 (GRCm38)	missense	probably damaging	1.00
R1997:Wdfy3	UTSW	5	101,968,946 (GRCm38)	missense	probably damaging	1.00
R2015:Wdfy3	UTSW	5	101,860,486 (GRCm38)	missense	probably null	1.00
R2087:Wdfy3	UTSW	5	101,895,060 (GRCm38)	missense	probably damaging	1.00
R2156:Wdfy3	UTSW	5	101,898,425 (GRCm38)	critical splice donor site	probably null
R2192:Wdfy3	UTSW	5	101,907,542 (GRCm38)	missense	possibly damaging	0.55
R2313:Wdfy3	UTSW	5	101,889,284 (GRCm38)	missense	probably damaging	1.00
R2332:Wdfy3	UTSW	5	101,888,323 (GRCm38)	splice site	probably benign
R2406:Wdfy3	UTSW	5	101,888,259 (GRCm38)	missense	probably damaging	1.00
R2679:Wdfy3	UTSW	5	101,870,036 (GRCm38)	missense	probably damaging	1.00
R2857:Wdfy3	UTSW	5	101,875,930 (GRCm38)	missense	probably benign	0.04
R2937:Wdfy3	UTSW	5	101,944,122 (GRCm38)	missense	probably benign	0.07
R3765:Wdfy3	UTSW	5	101,861,400 (GRCm38)	missense	probably damaging	1.00
R3795:Wdfy3	UTSW	5	101,937,600 (GRCm38)	missense	probably damaging	1.00
R3937:Wdfy3	UTSW	5	101,944,239 (GRCm38)	nonsense	probably null
R3947:Wdfy3	UTSW	5	101,870,036 (GRCm38)	missense	probably damaging	1.00
R4024:Wdfy3	UTSW	5	101,924,095 (GRCm38)	splice site	probably benign
R4065:Wdfy3	UTSW	5	101,922,447 (GRCm38)	missense	probably benign	0.08
R4066:Wdfy3	UTSW	5	101,922,447 (GRCm38)	missense	probably benign	0.08
R4110:Wdfy3	UTSW	5	101,900,058 (GRCm38)	critical splice donor site	probably null
R4235:Wdfy3	UTSW	5	101,922,634 (GRCm38)	critical splice acceptor site	probably null
R4420:Wdfy3	UTSW	5	101,910,984 (GRCm38)	missense	probably damaging	0.97
R4620:Wdfy3	UTSW	5	101,906,145 (GRCm38)	missense	probably damaging	0.99
R4624:Wdfy3	UTSW	5	101,884,083 (GRCm38)	missense	possibly damaging	0.52
R4626:Wdfy3	UTSW	5	101,943,934 (GRCm38)	missense	probably damaging	1.00
R4727:Wdfy3	UTSW	5	101,930,028 (GRCm38)	missense	probably damaging	0.99
R4794:Wdfy3	UTSW	5	101,943,943 (GRCm38)	missense	probably damaging	1.00
R4869:Wdfy3	UTSW	5	101,894,921 (GRCm38)	missense	probably damaging	0.98
R4971:Wdfy3	UTSW	5	101,948,972 (GRCm38)	nonsense	probably null
R4973:Wdfy3	UTSW	5	101,943,119 (GRCm38)	missense	probably benign	0.00
R4976:Wdfy3	UTSW	5	101,943,119 (GRCm38)	missense	probably benign	0.00
R4984:Wdfy3	UTSW	5	101,943,119 (GRCm38)	missense	probably benign	0.00
R4986:Wdfy3	UTSW	5	101,943,119 (GRCm38)	missense	probably benign	0.00
R5068:Wdfy3	UTSW	5	101,894,937 (GRCm38)	missense	probably benign	0.15
R5105:Wdfy3	UTSW	5	101,855,549 (GRCm38)	missense	probably damaging	1.00
R5120:Wdfy3	UTSW	5	101,868,106 (GRCm38)	missense	possibly damaging	0.85
R5134:Wdfy3	UTSW	5	101,944,103 (GRCm38)	missense	probably damaging	1.00
R5139:Wdfy3	UTSW	5	101,849,267 (GRCm38)	critical splice donor site	probably null
R5235:Wdfy3	UTSW	5	101,847,106 (GRCm38)	missense	probably null	0.03
R5303:Wdfy3	UTSW	5	101,952,983 (GRCm38)	missense	probably damaging	1.00
R5368:Wdfy3	UTSW	5	101,872,858 (GRCm38)	missense	probably damaging	1.00
R5426:Wdfy3	UTSW	5	101,919,446 (GRCm38)	missense	probably damaging	0.97
R5442:Wdfy3	UTSW	5	101,896,559 (GRCm38)	missense	probably benign	0.04
R5487:Wdfy3	UTSW	5	101,836,274 (GRCm38)	missense	probably damaging	1.00
R5509:Wdfy3	UTSW	5	101,861,448 (GRCm38)	missense	possibly damaging	0.69
R5988:Wdfy3	UTSW	5	101,884,138 (GRCm38)	missense	probably benign	0.00
R6017:Wdfy3	UTSW	5	101,851,359 (GRCm38)	missense	probably benign	0.01
R6019:Wdfy3	UTSW	5	101,849,423 (GRCm38)	missense	probably damaging	1.00
R6199:Wdfy3	UTSW	5	101,872,965 (GRCm38)	missense	possibly damaging	0.93
R6228:Wdfy3	UTSW	5	101,898,429 (GRCm38)	missense	possibly damaging	0.67
R6258:Wdfy3	UTSW	5	101,872,965 (GRCm38)	missense	possibly damaging	0.93
R6259:Wdfy3	UTSW	5	101,872,965 (GRCm38)	missense	possibly damaging	0.93
R6298:Wdfy3	UTSW	5	101,968,946 (GRCm38)	missense	probably damaging	1.00
R6479:Wdfy3	UTSW	5	101,913,179 (GRCm38)	missense	probably damaging	1.00
R6550:Wdfy3	UTSW	5	101,953,166 (GRCm38)	missense	probably benign	0.19
R6776:Wdfy3	UTSW	5	101,884,045 (GRCm38)	missense	possibly damaging	0.57
R6793:Wdfy3	UTSW	5	101,917,431 (GRCm38)	nonsense	probably null
R6809:Wdfy3	UTSW	5	101,923,947 (GRCm38)	missense	possibly damaging	0.63
R6836:Wdfy3	UTSW	5	101,952,999 (GRCm38)	missense	probably damaging	1.00
R6897:Wdfy3	UTSW	5	101,844,066 (GRCm38)	missense	probably benign	0.10
R7014:Wdfy3	UTSW	5	101,894,909 (GRCm38)	critical splice donor site	probably null
R7034:Wdfy3	UTSW	5	101,907,518 (GRCm38)	missense	probably damaging	1.00
R7035:Wdfy3	UTSW	5	101,855,549 (GRCm38)	missense	probably damaging	1.00
R7135:Wdfy3	UTSW	5	101,915,437 (GRCm38)	missense	probably damaging	1.00
R7182:Wdfy3	UTSW	5	101,943,892 (GRCm38)	missense	possibly damaging	0.51
R7217:Wdfy3	UTSW	5	101,901,919 (GRCm38)	missense	probably damaging	1.00
R7236:Wdfy3	UTSW	5	101,836,208 (GRCm38)	missense	probably damaging	0.99
R7264:Wdfy3	UTSW	5	101,855,523 (GRCm38)	missense	probably benign	0.02
R7418:Wdfy3	UTSW	5	101,957,500 (GRCm38)	missense	probably benign	0.08
R7533:Wdfy3	UTSW	5	101,882,488 (GRCm38)	missense	probably benign	0.27
R7543:Wdfy3	UTSW	5	101,936,059 (GRCm38)	missense	probably benign	0.00
R7625:Wdfy3	UTSW	5	101,855,386 (GRCm38)	splice site	probably null
R7788:Wdfy3	UTSW	5	101,848,357 (GRCm38)	missense	probably damaging	0.99
R7810:Wdfy3	UTSW	5	101,951,399 (GRCm38)	nonsense	probably null
R7810:Wdfy3	UTSW	5	101,895,074 (GRCm38)	missense	probably benign	0.01
R8204:Wdfy3	UTSW	5	101,852,585 (GRCm38)	missense	probably benign	0.00
R8268:Wdfy3	UTSW	5	101,941,610 (GRCm38)	missense	probably damaging	1.00
R8286:Wdfy3	UTSW	5	101,937,421 (GRCm38)	missense	probably benign
R8507:Wdfy3	UTSW	5	101,872,901 (GRCm38)	missense	probably benign	0.05
R8514:Wdfy3	UTSW	5	101,851,353 (GRCm38)	missense	possibly damaging	0.92
R8536:Wdfy3	UTSW	5	101,885,198 (GRCm38)	missense	probably benign
R8710:Wdfy3	UTSW	5	101,882,483 (GRCm38)	missense	probably damaging	1.00
R8735:Wdfy3	UTSW	5	101,930,085 (GRCm38)	missense	probably benign	0.00
R8749:Wdfy3	UTSW	5	101,882,580 (GRCm38)	missense	probably damaging	1.00
R8931:Wdfy3	UTSW	5	101,917,555 (GRCm38)	missense	probably benign	0.11
R8943:Wdfy3	UTSW	5	101,845,365 (GRCm38)	intron	probably benign
R8968:Wdfy3	UTSW	5	101,864,117 (GRCm38)	missense	probably benign	0.05
R8979:Wdfy3	UTSW	5	101,948,898 (GRCm38)	missense	probably damaging	1.00
R8998:Wdfy3	UTSW	5	101,845,192 (GRCm38)	missense	probably benign	0.05
R9045:Wdfy3	UTSW	5	101,847,174 (GRCm38)	missense	probably damaging	1.00
R9068:Wdfy3	UTSW	5	101,852,585 (GRCm38)	missense	probably benign	0.34
R9105:Wdfy3	UTSW	5	101,882,646 (GRCm38)	missense	probably benign	0.05
R9122:Wdfy3	UTSW	5	101,943,965 (GRCm38)	missense	probably damaging	1.00
R9209:Wdfy3	UTSW	5	101,930,964 (GRCm38)	missense	probably benign	0.01
R9249:Wdfy3	UTSW	5	101,848,493 (GRCm38)	missense	possibly damaging	0.82
R9348:Wdfy3	UTSW	5	101,941,492 (GRCm38)	missense	probably damaging	1.00
R9481:Wdfy3	UTSW	5	101,852,612 (GRCm38)	missense	probably benign	0.19
R9490:Wdfy3	UTSW	5	101,930,850 (GRCm38)	missense	probably benign	0.29
R9524:Wdfy3	UTSW	5	101,907,467 (GRCm38)	missense	probably benign	0.03
R9545:Wdfy3	UTSW	5	101,953,091 (GRCm38)	missense
R9548:Wdfy3	UTSW	5	101,885,193 (GRCm38)	missense	probably damaging	0.99
R9636:Wdfy3	UTSW	5	101,900,033 (GRCm38)	missense	probably benign
R9750:Wdfy3	UTSW	5	101,930,094 (GRCm38)	missense	probably benign	0.00
R9766:Wdfy3	UTSW	5	101,895,000 (GRCm38)	missense	possibly damaging	0.90
R9771:Wdfy3	UTSW	5	101,852,329 (GRCm38)	missense	probably damaging	1.00
Z1177:Wdfy3	UTSW	5	101,900,241 (GRCm38)	missense	probably benign	0.39

Predicted Primers

PCR Primer
(F):5'- ACTGGGAGGTGACTTAGGAC -3'
(R):5'- TGAACCCGGGTTTAACATGC -3'

Sequencing Primer
(F):5'- ACTTAGGACATGGCAGTGTC -3'
(R):5'- GAACCCGGGTTTAACATGCTTAGC -3'

Posted On

2017-02-10