Mutagenetix > Incidental Mutation

Incidental Mutation 'R5119:Spag9'

455760

Institutional Source

Beutler Lab

Gene Symbol

Spag9

Ensembl Gene

ENSMUSG00000020859

Gene Name

sperm associated antigen 9

Synonyms

syd1, JIP4, Mapk8ip4, 4733401I23Rik, JLP, 3110018C07Rik, 4831406C20Rik

MMRRC Submission

042707-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.784) question?

Stock #

R5119 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

93996091-94126085 bp(+) (GRCm38)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 94122722 bp (GRCm38)

Zygosity

Heterozygous

Amino Acid Change

Glycine to Aspartic acid at position 1127 (G1127D)

Ref Sequence

ENSEMBL: ENSMUSP00000099457 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000024979] [ENSMUST00000041956] [ENSMUST00000075695] [ENSMUST00000092777] [ENSMUST00000103168] [ENSMUST00000153076]

AlphaFold

no structure available at present

Predicted Effect

probably damaging
Transcript: ENSMUST00000024979
AA Change: G1132D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000024979
Gene: ENSMUSG00000020859
AA Change: G1132D

Domain	Start	End	E-Value	Type
transmembrane domain	7	29	N/A	INTRINSIC
PDB:2W83\|D	253	305	1e-25	PDB
low complexity region	306	339	N/A	INTRINSIC
coiled coil region	572	606	N/A	INTRINSIC
low complexity region	735	751	N/A	INTRINSIC
SCOP:d1kb0a2	823	969	3e-5	SMART
Blast:WD40	924	964	8e-18	BLAST
low complexity region	1132	1150	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000041956
AA Change: G1270D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000042271
Gene: ENSMUSG00000020859
AA Change: G1270D

Domain	Start	End	E-Value	Type
Pfam:Jnk-SapK_ap_N	24	179	2e-61	PFAM
Pfam:JIP_LZII	390	460	5.3e-32	PFAM
coiled coil region	710	744	N/A	INTRINSIC
low complexity region	873	889	N/A	INTRINSIC
SCOP:d1kb0a2	961	1107	1e-5	SMART
Blast:WD40	1062	1102	1e-17	BLAST
low complexity region	1270	1288	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000075695
AA Change: G1131D

PolyPhen 2 Score 0.999 (Sensitivity: 0.14; Specificity: 0.99)

SMART Domains

Protein: ENSMUSP00000075115
Gene: ENSMUSG00000020859
AA Change: G1131D

Domain	Start	End	E-Value	Type
transmembrane domain	7	29	N/A	INTRINSIC
PDB:2W83\|D	253	305	1e-25	PDB
low complexity region	306	339	N/A	INTRINSIC
coiled coil region	571	605	N/A	INTRINSIC
low complexity region	734	750	N/A	INTRINSIC
SCOP:d1kb0a2	822	968	3e-5	SMART
Blast:WD40	923	963	7e-18	BLAST
low complexity region	1131	1149	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000092777
AA Change: G1132D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000090452
Gene: ENSMUSG00000020859
AA Change: G1132D

Domain	Start	End	E-Value	Type
transmembrane domain	7	29	N/A	INTRINSIC
PDB:2W83\|D	254	306	1e-25	PDB
low complexity region	307	340	N/A	INTRINSIC
coiled coil region	572	606	N/A	INTRINSIC
low complexity region	735	751	N/A	INTRINSIC
SCOP:d1kb0a2	823	969	3e-5	SMART
Blast:WD40	924	964	7e-18	BLAST
low complexity region	1132	1150	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000103168
AA Change: G1127D

PolyPhen 2 Score 1.000 (Sensitivity: 0.00; Specificity: 1.00)

SMART Domains

Protein: ENSMUSP00000099457
Gene: ENSMUSG00000020859
AA Change: G1127D

Domain	Start	End	E-Value	Type
transmembrane domain	7	29	N/A	INTRINSIC
PDB:2W83\|D	249	301	1e-25	PDB
low complexity region	302	335	N/A	INTRINSIC
coiled coil region	567	601	N/A	INTRINSIC
low complexity region	730	746	N/A	INTRINSIC
SCOP:d1kb0a2	818	964	3e-5	SMART
Blast:WD40	919	959	8e-18	BLAST
low complexity region	1127	1145	N/A	INTRINSIC

Predicted Effect

probably damaging
Transcript: ENSMUST00000153076
AA Change: G864D

PolyPhen 2 Score 0.977 (Sensitivity: 0.76; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000117502
Gene: ENSMUSG00000020859
AA Change: G864D

Domain	Start	End	E-Value	Type
PDB:2W83\|D	1	25	4e-8	PDB
low complexity region	26	59	N/A	INTRINSIC
coiled coil region	291	325	N/A	INTRINSIC
low complexity region	454	470	N/A	INTRINSIC
SCOP:d1kb0a2	542	688	3e-5	SMART
Blast:WD40	643	683	1e-17	BLAST
low complexity region	864	882	N/A	INTRINSIC

Meta Mutation Damage Score

0.1431

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.1%

Validation Efficiency

100% (127/127)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a member of the cancer testis antigen gene family. The encoded protein functions as a scaffold protein that structurally organizes mitogen-activated protein kinases and mediates c-Jun-terminal kinase signaling. This protein also binds to kinesin-1 and may be involved in microtubule-based membrane transport. This protein may play a role in tumor growth and development. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2011]
PHENOTYPE: Male mice homozygous for a null mutation display reduced fertility with oligoasthenozoospermia. [provided by MGI curators]

Allele List at MGI

All alleles(5) : Targeted, knock-out(1) Gene trapped(4)

Other mutations in this stock

Total: 120 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4930470P17Rik	C	A	2: 170,579,585 (GRCm38)	G125V	unknown	Het
Actr6	A	T	10: 89,725,855 (GRCm38)	L143Q	probably damaging	Het
Adamts18	A	G	8: 113,699,010 (GRCm38)	Y1207H	probably benign	Het
Adamts5	T	A	16: 85,899,578 (GRCm38)	R230S	probably benign	Het
Adat2	A	T	10: 13,556,906 (GRCm38)	N51Y	probably damaging	Het
Adgrv1	T	C	13: 81,419,427 (GRCm38)	Y5209C	possibly damaging	Het
Ahnak	G	T	19: 9,013,644 (GRCm38)	M4097I	probably benign	Het
Akap13	A	G	7: 75,687,252 (GRCm38)	T820A	probably damaging	Het
Als2cl	C	T	9: 110,890,819 (GRCm38)	R492C	probably damaging	Het
Aox3	G	A	1: 58,188,524 (GRCm38)		probably null	Het
Arid4b	G	A	13: 14,164,281 (GRCm38)	V446M	probably benign	Het
Armc2	A	G	10: 41,922,148 (GRCm38)	L794P	probably damaging	Het
Atp6v0a1	T	A	11: 101,020,515 (GRCm38)	M80K	probably benign	Het
Aup1	T	C	6: 83,055,134 (GRCm38)	V94A	probably damaging	Het
Bank1	A	T	3: 136,234,682 (GRCm38)	I180K	possibly damaging	Het
Becn1	A	G	11: 101,291,395 (GRCm38)	L116P	probably damaging	Het
Bsg	A	G	10: 79,710,223 (GRCm38)		probably benign	Het
Camk2a	A	T	18: 60,943,136 (GRCm38)		probably benign	Het
Ccdc180	A	G	4: 45,914,603 (GRCm38)	E706G	possibly damaging	Het
Cdk8	A	T	5: 146,283,627 (GRCm38)		probably null	Het
Cpne4	A	G	9: 104,901,521 (GRCm38)		probably null	Het
Cspp1	T	A	1: 10,126,463 (GRCm38)	N900K	probably damaging	Het
Cyp2c38	C	A	19: 39,460,621 (GRCm38)	G96V	probably damaging	Het
Dhx40	A	G	11: 86,776,636 (GRCm38)	I261T	probably damaging	Het
Dnah10	T	C	5: 124,779,258 (GRCm38)	F2038L	probably damaging	Het
Dnah7a	A	T	1: 53,698,692 (GRCm38)	D27E	probably benign	Het
Dock10	A	T	1: 80,567,994 (GRCm38)		probably null	Het
Dst	A	T	1: 34,195,969 (GRCm38)	K3710*	probably null	Het
Dtl	G	T	1: 191,541,506 (GRCm38)	A430D	probably damaging	Het
Ece2	T	C	16: 20,618,631 (GRCm38)	L241P	probably damaging	Het
Ecel1	T	A	1: 87,151,139 (GRCm38)	Y526F	probably benign	Het
Enpp2	T	A	15: 54,870,305 (GRCm38)	R420*	probably null	Het
Epb41	A	C	4: 131,937,436 (GRCm38)		probably benign	Het
Eppin	T	C	2: 164,589,451 (GRCm38)	Y85C	probably damaging	Het
Fam171a2	G	A	11: 102,438,733 (GRCm38)	A400V	probably damaging	Het
Fam71b	G	A	11: 46,407,036 (GRCm38)	G389D	probably damaging	Het
Fem1c	A	C	18: 46,506,369 (GRCm38)	C189G	probably damaging	Het
Frmd4b	A	G	6: 97,300,314 (GRCm38)	V560A	probably benign	Het
Fsip2	A	T	2: 82,988,191 (GRCm38)	D4756V	probably damaging	Het
Gabbr1	T	C	17: 37,048,438 (GRCm38)	S102P	probably damaging	Het
Gm28042	G	A	2: 120,034,643 (GRCm38)	A250T	probably damaging	Het
Gm42877	T	C	14: 54,075,521 (GRCm38)		probably benign	Het
Gm9762	A	T	3: 78,966,400 (GRCm38)		noncoding transcript	Het
Gpr183	G	T	14: 121,954,863 (GRCm38)	T82N	possibly damaging	Het
Gps2	A	G	11: 69,914,791 (GRCm38)	K45R	probably benign	Het
Gramd2	A	G	9: 59,714,320 (GRCm38)		probably benign	Het
Grap2	G	A	15: 80,646,144 (GRCm38)	R155Q	possibly damaging	Het
Hsdl1	T	A	8: 119,565,867 (GRCm38)	Y203F	possibly damaging	Het
Ifi204	A	T	1: 173,755,668 (GRCm38)	I328N	probably damaging	Het
Igsf3	T	A	3: 101,439,361 (GRCm38)		probably null	Het
Il1rap	T	C	16: 26,624,199 (GRCm38)	I15T	probably benign	Het
Il23r	A	T	6: 67,466,316 (GRCm38)	C268S	probably damaging	Het
Irx4	A	G	13: 73,268,921 (GRCm38)	T479A	probably benign	Het
Kcnk7	G	A	19: 5,706,324 (GRCm38)	V193I	probably benign	Het
Kcnt1	G	T	2: 25,909,322 (GRCm38)		probably benign	Het
Kif13b	T	G	14: 64,757,453 (GRCm38)	C885G	probably benign	Het
Kif21b	C	A	1: 136,163,100 (GRCm38)	D1215E	probably benign	Het
Klhdc2	A	G	12: 69,296,962 (GRCm38)		probably benign	Het
Kmt2d	G	A	15: 98,847,194 (GRCm38)		probably benign	Het
Lama4	A	G	10: 39,048,054 (GRCm38)	N486S	probably benign	Het
Ldah	G	A	12: 8,227,237 (GRCm38)	A58T	probably benign	Het
Lrrc24	T	C	15: 76,716,000 (GRCm38)	Q313R	probably benign	Het
Luzp1	A	G	4: 136,543,397 (GRCm38)	D977G	possibly damaging	Het
Mrgpra4	A	G	7: 47,981,718 (GRCm38)	L45P	probably damaging	Het
Mrps28	C	T	3: 8,923,696 (GRCm38)	G34D	possibly damaging	Het
Myh8	A	G	11: 67,298,358 (GRCm38)	E1120G	probably damaging	Het
Myt1l	A	G	12: 29,832,303 (GRCm38)	E499G	unknown	Het
Ncan	C	T	8: 70,115,025 (GRCm38)	E146K	probably damaging	Het
Nlgn1	A	T	3: 25,433,794 (GRCm38)	D763E	probably damaging	Het
Olfr1259	A	T	2: 89,943,803 (GRCm38)	I104N	possibly damaging	Het
Olfr1474	C	T	19: 13,471,546 (GRCm38)	T192I	probably benign	Het
Olfr157	G	T	4: 43,836,433 (GRCm38)	A19D	probably benign	Het
Olfr23	A	T	11: 73,940,552 (GRCm38)	Y102F	possibly damaging	Het
Olfr742	T	C	14: 50,515,509 (GRCm38)	F102L	probably benign	Het
Olfr765	T	A	10: 129,046,842 (GRCm38)	I74F	possibly damaging	Het
Pak6	A	T	2: 118,694,548 (GRCm38)	I552F	probably damaging	Het
Pcbp1	G	A	6: 86,524,915 (GRCm38)	A334V	probably damaging	Het
Pclaf	T	C	9: 65,890,780 (GRCm38)	V32A	probably benign	Het
Pga5	T	A	19: 10,676,689 (GRCm38)	H50L	probably benign	Het
Phyhipl	A	T	10: 70,569,074 (GRCm38)	D56E	probably damaging	Het
Pik3ap1	C	T	19: 41,281,976 (GRCm38)	R758H	probably benign	Het
Plekha1	A	G	7: 130,905,364 (GRCm38)		probably benign	Het
Plekhm1	A	T	11: 103,387,315 (GRCm38)	N318K	possibly damaging	Het
Ppargc1b	G	A	18: 61,307,654 (GRCm38)	A731V	probably benign	Het
Pptc7	T	C	5: 122,313,781 (GRCm38)	V100A	possibly damaging	Het
Prl7a1	A	T	13: 27,633,581 (GRCm38)	H233Q	possibly damaging	Het
Prpf40a	A	G	2: 53,144,849 (GRCm38)	F776L	probably damaging	Het
Prrc2c	T	C	1: 162,705,440 (GRCm38)		probably benign	Het
Psmb1	T	C	17: 15,498,262 (GRCm38)	M1V	probably null	Het
Ptpn3	A	G	4: 57,218,513 (GRCm38)	F650S	possibly damaging	Het
Ranbp17	A	T	11: 33,404,181 (GRCm38)	*577R	probably null	Het
Reln	T	A	5: 21,971,870 (GRCm38)	N1933Y	probably benign	Het
Rgl2	T	A	17: 33,937,120 (GRCm38)	H727Q	probably benign	Het
Rhpn2	A	G	7: 35,371,124 (GRCm38)	T160A	probably damaging	Het
Rlf	G	A	4: 121,147,455 (GRCm38)	H1443Y	probably damaging	Het
Rpgrip1l	T	C	8: 91,280,816 (GRCm38)	E382G	probably damaging	Het
Rxrb	T	C	17: 34,033,588 (GRCm38)	S50P	probably benign	Het
Scn4b	A	T	9: 45,147,758 (GRCm38)	E109V	probably damaging	Het
Scrib	C	T	15: 76,051,753 (GRCm38)		probably null	Het
Slc22a29	A	T	19: 8,217,830 (GRCm38)	V147D	probably damaging	Het
Slc4a4	A	T	5: 88,954,862 (GRCm38)	E53V	probably null	Het
Slx4	A	G	16: 4,001,199 (GRCm38)	S37P	possibly damaging	Het
Srek1	T	A	13: 103,752,556 (GRCm38)		probably benign	Het
Supt5	G	T	7: 28,316,370 (GRCm38)	P849Q	probably damaging	Het
Tex14	T	C	11: 87,433,813 (GRCm38)	S2P	probably damaging	Het
Them7	A	G	2: 105,378,808 (GRCm38)	T158A	probably benign	Het
Tldc1	A	C	8: 119,768,143 (GRCm38)	L292R	probably damaging	Het
Tll2	A	G	19: 41,130,509 (GRCm38)	V260A	possibly damaging	Het
Tlr6	A	G	5: 64,954,301 (GRCm38)	V421A	probably benign	Het
Tmc3	T	G	7: 83,615,010 (GRCm38)	C649G	probably damaging	Het
Tnn	A	G	1: 160,120,552 (GRCm38)	W864R	probably damaging	Het
Tsc2	A	C	17: 24,603,280 (GRCm38)	V1095G	probably benign	Het
Vmn1r124	C	T	7: 21,260,247 (GRCm38)	G124D	probably damaging	Het
Vmn2r116	T	C	17: 23,387,164 (GRCm38)	V350A	probably benign	Het
Vps13d	A	G	4: 145,105,898 (GRCm38)	S2813P	possibly damaging	Het
Wrap53	T	A	11: 69,563,932 (GRCm38)	M204L	possibly damaging	Het
Zfp277	A	G	12: 40,328,688 (GRCm38)	V390A	possibly damaging	Het
Zfp687	A	T	3: 95,011,676 (GRCm38)	S262T	probably benign	Het
Zfp831	T	A	2: 174,705,310 (GRCm38)	S1429T	probably benign	Het
Znfx1	G	A	2: 167,065,387 (GRCm38)		probably benign	Het

Other mutations in Spag9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00422:Spag9	APN	11	94,097,866 (GRCm38)	missense	probably benign	0.02
IGL01776:Spag9	APN	11	94,116,727 (GRCm38)	splice site	probably benign
IGL02095:Spag9	APN	11	94,108,582 (GRCm38)	missense	probably damaging	1.00
IGL02307:Spag9	APN	11	94,102,160 (GRCm38)	critical splice donor site	probably null
IGL02417:Spag9	APN	11	94,116,741 (GRCm38)	missense	probably benign	0.27
IGL02480:Spag9	APN	11	94,108,587 (GRCm38)	nonsense	probably null
IGL02864:Spag9	APN	11	94,106,661 (GRCm38)	missense	probably damaging	1.00
IGL02976:Spag9	APN	11	94,083,953 (GRCm38)	missense	probably benign	0.30
IGL02979:Spag9	APN	11	94,097,364 (GRCm38)	missense	probably benign
IGL03349:Spag9	APN	11	94,093,509 (GRCm38)	missense	possibly damaging	0.51
dazzle	UTSW	11	94,093,624 (GRCm38)	nonsense	probably null
R0128:Spag9	UTSW	11	94,093,539 (GRCm38)	missense	probably damaging	1.00
R0418:Spag9	UTSW	11	94,091,753 (GRCm38)	splice site	probably benign
R1463:Spag9	UTSW	11	94,116,837 (GRCm38)	missense	probably damaging	1.00
R1593:Spag9	UTSW	11	94,097,233 (GRCm38)	missense	probably damaging	1.00
R1605:Spag9	UTSW	11	94,048,539 (GRCm38)	missense	probably damaging	0.99
R1649:Spag9	UTSW	11	94,108,452 (GRCm38)	splice site	probably null
R1697:Spag9	UTSW	11	93,996,565 (GRCm38)	missense	probably benign	0.00
R1952:Spag9	UTSW	11	94,097,358 (GRCm38)	missense	possibly damaging	0.77
R2011:Spag9	UTSW	11	94,092,375 (GRCm38)	nonsense	probably null
R2012:Spag9	UTSW	11	94,092,375 (GRCm38)	nonsense	probably null
R2351:Spag9	UTSW	11	94,092,900 (GRCm38)	missense	probably damaging	1.00
R2367:Spag9	UTSW	11	94,116,757 (GRCm38)	missense	probably damaging	1.00
R3027:Spag9	UTSW	11	94,086,377 (GRCm38)	missense	probably null	1.00
R3766:Spag9	UTSW	11	94,060,283 (GRCm38)	intron	probably benign
R3777:Spag9	UTSW	11	94,099,026 (GRCm38)	critical splice acceptor site	probably null
R3937:Spag9	UTSW	11	94,044,479 (GRCm38)	missense	possibly damaging	0.92
R3937:Spag9	UTSW	11	94,044,417 (GRCm38)	missense	possibly damaging	0.94
R4417:Spag9	UTSW	11	94,060,346 (GRCm38)	intron	probably benign
R4445:Spag9	UTSW	11	94,097,253 (GRCm38)	missense	possibly damaging	0.95
R4711:Spag9	UTSW	11	94,114,351 (GRCm38)	critical splice donor site	probably null
R4799:Spag9	UTSW	11	94,048,517 (GRCm38)	missense	probably damaging	0.96
R4799:Spag9	UTSW	11	94,048,516 (GRCm38)	missense	possibly damaging	0.87
R4816:Spag9	UTSW	11	94,048,599 (GRCm38)	intron	probably benign
R4843:Spag9	UTSW	11	94,097,818 (GRCm38)	missense	probably damaging	1.00
R5020:Spag9	UTSW	11	94,097,786 (GRCm38)	missense	probably benign	0.08
R5298:Spag9	UTSW	11	94,100,135 (GRCm38)	missense	probably damaging	1.00
R5304:Spag9	UTSW	11	94,069,012 (GRCm38)	missense	probably damaging	1.00
R5305:Spag9	UTSW	11	94,069,012 (GRCm38)	missense	probably damaging	1.00
R5395:Spag9	UTSW	11	94,091,751 (GRCm38)	splice site	probably null
R5636:Spag9	UTSW	11	94,069,012 (GRCm38)	missense	probably damaging	1.00
R5638:Spag9	UTSW	11	94,069,012 (GRCm38)	missense	probably damaging	1.00
R5654:Spag9	UTSW	11	94,090,712 (GRCm38)	missense	probably damaging	1.00
R5779:Spag9	UTSW	11	94,114,253 (GRCm38)	missense	probably benign	0.20
R5814:Spag9	UTSW	11	94,082,828 (GRCm38)	missense	possibly damaging	0.94
R5912:Spag9	UTSW	11	94,044,425 (GRCm38)	missense	probably damaging	0.98
R6038:Spag9	UTSW	11	94,112,092 (GRCm38)	missense	probably damaging	1.00
R6038:Spag9	UTSW	11	94,112,092 (GRCm38)	missense	probably damaging	1.00
R6269:Spag9	UTSW	11	94,044,507 (GRCm38)	missense	probably benign	0.05
R6294:Spag9	UTSW	11	94,093,485 (GRCm38)	critical splice acceptor site	probably null
R6389:Spag9	UTSW	11	94,086,311 (GRCm38)	missense	probably damaging	1.00
R6420:Spag9	UTSW	11	94,086,302 (GRCm38)	missense	probably damaging	1.00
R6460:Spag9	UTSW	11	94,068,975 (GRCm38)	missense	probably damaging	1.00
R6482:Spag9	UTSW	11	94,093,502 (GRCm38)	missense	possibly damaging	0.94
R6860:Spag9	UTSW	11	94,081,370 (GRCm38)	missense	probably benign	0.25
R7086:Spag9	UTSW	11	94,097,864 (GRCm38)	missense	probably benign
R7179:Spag9	UTSW	11	94,089,432 (GRCm38)	splice site	probably null
R7225:Spag9	UTSW	11	94,097,358 (GRCm38)	missense	probably damaging	0.98
R7351:Spag9	UTSW	11	94,092,976 (GRCm38)	missense	probably benign	0.00
R7366:Spag9	UTSW	11	94,108,521 (GRCm38)	missense	possibly damaging	0.56
R7378:Spag9	UTSW	11	94,114,351 (GRCm38)	critical splice donor site	probably null
R7401:Spag9	UTSW	11	94,097,689 (GRCm38)	missense	probably benign
R7506:Spag9	UTSW	11	94,108,464 (GRCm38)	missense	probably damaging	1.00
R7507:Spag9	UTSW	11	94,068,080 (GRCm38)	missense	probably benign	0.00
R7513:Spag9	UTSW	11	94,112,083 (GRCm38)	missense	probably damaging	1.00
R7655:Spag9	UTSW	11	93,996,563 (GRCm38)	missense	possibly damaging	0.56
R7656:Spag9	UTSW	11	93,996,563 (GRCm38)	missense	possibly damaging	0.56
R7664:Spag9	UTSW	11	94,102,160 (GRCm38)	critical splice donor site	probably null
R7665:Spag9	UTSW	11	94,013,654 (GRCm38)	missense	probably damaging	0.98
R7862:Spag9	UTSW	11	94,112,066 (GRCm38)	missense	possibly damaging	0.69
R8074:Spag9	UTSW	11	94,112,051 (GRCm38)	missense	probably damaging	1.00
R8085:Spag9	UTSW	11	94,099,044 (GRCm38)	missense	probably benign
R8469:Spag9	UTSW	11	94,091,801 (GRCm38)	missense	probably damaging	1.00
R8547:Spag9	UTSW	11	94,122,821 (GRCm38)	missense	possibly damaging	0.84
R8709:Spag9	UTSW	11	94,068,090 (GRCm38)	missense	probably benign	0.02
R8732:Spag9	UTSW	11	94,071,688 (GRCm38)	critical splice donor site	probably null
R8899:Spag9	UTSW	11	94,092,869 (GRCm38)	missense	probably damaging	1.00
R8983:Spag9	UTSW	11	94,067,989 (GRCm38)	missense	probably benign
R9043:Spag9	UTSW	11	94,060,259 (GRCm38)	missense
R9050:Spag9	UTSW	11	94,044,468 (GRCm38)	missense	probably damaging	0.97
R9502:Spag9	UTSW	11	94,068,966 (GRCm38)	missense	probably damaging	1.00
R9575:Spag9	UTSW	11	94,071,583 (GRCm38)	missense	probably damaging	0.99
R9667:Spag9	UTSW	11	93,996,293 (GRCm38)	missense	possibly damaging	0.83
R9683:Spag9	UTSW	11	94,097,742 (GRCm38)	missense	probably damaging	1.00
R9774:Spag9	UTSW	11	94,114,236 (GRCm38)	missense	probably damaging	0.96

Predicted Primers

PCR Primer
(F):5'- TCCAGCATACTATTGTGAACTAGC -3'
(R):5'- CAGTAAACTAGCTCGCTCCTC -3'

Sequencing Primer
(F):5'- ATGGCTATGAGCTGCCTAAC -3'
(R):5'- GCTCCTCTGTATTGTTACCATAGAAC -3'

Posted On

2017-02-13