Mutagenetix > Incidental Mutation

Incidental Mutation 'R5264:Ppp1r35'

455974

Institutional Source

Beutler Lab

Gene Symbol

Ppp1r35

Ensembl Gene

ENSMUSG00000029725

Gene Name

protein phosphatase 1, regulatory subunit 35

Synonyms

2010007H12Rik, 2010011D20Rik

MMRRC Submission

042832-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R5264 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

137777138-137778372 bp(+) (GRCm39)

Type of Mutation

unclassified

DNA Base Change (assembly)

G to A at 137778286 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000143201 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000031739] [ENSMUST00000031740] [ENSMUST00000196022] [ENSMUST00000198929]

AlphaFold

Q9D8C8

Predicted Effect

silent
Transcript: ENSMUST00000031739

SMART Domains

Protein: ENSMUSP00000031739
Gene: ENSMUSG00000029725

Domain	Start	End	E-Value	Type
low complexity region	13	54	N/A	INTRINSIC
low complexity region	57	76	N/A	INTRINSIC
Pfam:PPP1R35_C	107	255	1.5e-48	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000031740

SMART Domains

Protein: ENSMUSP00000031740
Gene: ENSMUSG00000029726

Domain	Start	End	E-Value	Type
low complexity region	14	19	N/A	INTRINSIC
low complexity region	49	74	N/A	INTRINSIC
low complexity region	115	121	N/A	INTRINSIC
low complexity region	130	136	N/A	INTRINSIC
low complexity region	234	254	N/A	INTRINSIC
low complexity region	307	352	N/A	INTRINSIC
low complexity region	355	376	N/A	INTRINSIC
Pfam:Methyltransf_23	398	623	2.7e-14	PFAM
Pfam:PrmA	408	489	6.9e-6	PFAM
Pfam:Methyltransf_31	419	480	9.3e-9	PFAM
Pfam:Methyltransf_18	420	595	1.8e-13	PFAM
Pfam:Bin3	552	660	4.2e-45	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000126126

SMART Domains

Protein: ENSMUSP00000114480
Gene: ENSMUSG00000029725

Domain	Start	End	E-Value	Type
Pfam:PPP1R35_C	48	144	2.5e-32	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000132726

SMART Domains

Protein: ENSMUSP00000118688
Gene: ENSMUSG00000029726

Domain	Start	End	E-Value	Type
Pfam:Methyltransf_11	33	99	8e-6	PFAM
Pfam:Bin3	59	167	8.3e-47	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000140336

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000152298

Predicted Effect

probably benign
Transcript: ENSMUST00000196022

Predicted Effect

unknown
Transcript: ENSMUST00000198929
AA Change: V94I

SMART Domains

Protein: ENSMUSP00000143182
Gene: ENSMUSG00000029725
AA Change: V94I

Domain	Start	End	E-Value	Type
low complexity region	66	77	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000196936

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 97.0%
20x: 94.8%

Validation Efficiency

98% (54/55)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ckap2l	A	T	2: 129,127,299 (GRCm39)	M293K	probably benign	Het
Col4a4	C	T	1: 82,471,312 (GRCm39)	G681E	unknown	Het
Dnai7	A	G	6: 145,127,502 (GRCm39)	V469A	probably benign	Het
Efcab7	G	A	4: 99,735,372 (GRCm39)	R132H	probably benign	Het
Elovl4	T	C	9: 83,662,817 (GRCm39)	T239A	probably benign	Het
Fank1	A	G	7: 133,481,621 (GRCm39)	D240G	probably damaging	Het
Fbln5	T	A	12: 101,723,703 (GRCm39)	M346L	possibly damaging	Het
Fbxl21	T	C	13: 56,680,136 (GRCm39)	F174L	probably benign	Het
Gns	A	G	10: 121,216,090 (GRCm39)	D279G	probably benign	Het
Hmcn1	A	G	1: 150,555,265 (GRCm39)	V2502A	probably benign	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Large2	A	G	2: 92,205,088 (GRCm39)		probably benign	Het
Lrrc8b	A	T	5: 105,628,118 (GRCm39)	I155F	probably damaging	Het
Morc2b	T	C	17: 33,357,353 (GRCm39)	I140V	probably benign	Het
Mrgprb5	G	A	7: 47,817,796 (GRCm39)	S313L	probably benign	Het
Nectin4	A	T	1: 171,211,273 (GRCm39)	T266S	probably benign	Het
Nsd1	C	T	13: 55,395,159 (GRCm39)	A1023V	possibly damaging	Het
Or51a39	T	C	7: 102,363,558 (GRCm39)	T21A	probably benign	Het
Paqr8	A	G	1: 21,005,332 (GRCm39)	H162R	possibly damaging	Het
Pclo	G	A	5: 14,726,937 (GRCm39)		probably benign	Het
Phactr4	T	C	4: 132,098,293 (GRCm39)	D325G	probably damaging	Het
Plcg2	A	T	8: 118,361,532 (GRCm39)	E1255V	possibly damaging	Het
Polr3a	T	C	14: 24,505,009 (GRCm39)	I1084V	possibly damaging	Het
Polr3b	A	T	10: 84,503,280 (GRCm39)	Q399L	probably benign	Het
Psd3	A	T	8: 68,166,377 (GRCm39)	D919E	probably benign	Het
Ptgs2	A	G	1: 149,978,481 (GRCm39)	T198A	possibly damaging	Het
Ptpn14	T	C	1: 189,564,997 (GRCm39)		probably null	Het
Ptprk	A	G	10: 28,461,582 (GRCm39)	Y39C	probably damaging	Het
R3hdm4	A	G	10: 79,749,175 (GRCm39)	Y75H	probably benign	Het
Rsph4a	A	G	10: 33,785,379 (GRCm39)	Y430C	probably damaging	Het
Samd12	C	T	15: 53,723,669 (GRCm39)	C8Y	probably damaging	Het
Sema3e	A	C	5: 14,276,662 (GRCm39)	L314F	probably damaging	Het
Sis	A	G	3: 72,857,089 (GRCm39)	F401L	probably damaging	Het
Smoc1	T	A	12: 81,151,474 (GRCm39)	S64T	probably damaging	Het
Socs5	T	A	17: 87,441,769 (GRCm39)	H236Q	probably damaging	Het
Spaca1	C	A	4: 34,049,863 (GRCm39)	R45L	possibly damaging	Het
Spag6	A	G	2: 18,750,324 (GRCm39)	K457E	probably benign	Het
Stat2	T	A	10: 128,116,934 (GRCm39)		probably null	Het
Tcp11l2	A	G	10: 84,449,524 (GRCm39)	I496M	probably damaging	Het
Ttll4	A	G	1: 74,725,535 (GRCm39)	I648V	possibly damaging	Het
Vmn2r67	T	C	7: 84,801,453 (GRCm39)	Y161C	probably damaging	Het
Wnt5b	A	T	6: 119,410,813 (GRCm39)	V171E	probably damaging	Het
Zfp236	T	C	18: 82,648,219 (GRCm39)	K933E	probably damaging	Het
Zfp236	T	C	18: 82,676,198 (GRCm39)	E373G	probably damaging	Het
Zfp617	A	G	8: 72,686,885 (GRCm39)	Y405C	probably damaging	Het
Zranb1	CTGATGATGATG	CTGATGATGATGATG	7: 132,584,556 (GRCm39)		probably benign	Het

Other mutations in Ppp1r35

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00325:Ppp1r35	APN	5	137,777,799 (GRCm39)	missense	probably damaging	0.98
IGL02015:Ppp1r35	APN	5	137,778,293 (GRCm39)	unclassified	probably benign
R2091:Ppp1r35	UTSW	5	137,778,156 (GRCm39)	missense	possibly damaging	0.61
R2155:Ppp1r35	UTSW	5	137,778,267 (GRCm39)	missense	probably benign	0.01
R5249:Ppp1r35	UTSW	5	137,777,406 (GRCm39)	unclassified	probably benign
R7436:Ppp1r35	UTSW	5	137,778,279 (GRCm39)	nonsense	probably null
R8206:Ppp1r35	UTSW	5	137,778,296 (GRCm39)	missense	unknown
R8803:Ppp1r35	UTSW	5	137,777,731 (GRCm39)	missense	possibly damaging	0.93
R9389:Ppp1r35	UTSW	5	137,777,577 (GRCm39)	missense	probably damaging	1.00
R9524:Ppp1r35	UTSW	5	137,777,304 (GRCm39)	missense	unknown
Z1177:Ppp1r35	UTSW	5	137,777,205 (GRCm39)	unclassified	probably benign

Predicted Primers

Posted On

2017-02-14